Purpose: This study aims to investigate the predictive value of blood calcium in the prognosis of traumatic fracture. Methods: A retrospective experimental design was employed, 112 cases (52 non-fracture and 60 trauma...Purpose: This study aims to investigate the predictive value of blood calcium in the prognosis of traumatic fracture. Methods: A retrospective experimental design was employed, 112 cases (52 non-fracture and 60 traumatic fracture) were randomly selected. The type of fracture complies with WHO-recommended (2019) diagnostic criteria for osteoporosis combined with fracture. The blood pressure (BP) was measured by OMRON’s HEM-7136 model electronic blood pressure monitor. Blood calcium (Ca<sup>2+</sup>), and blood phosphorus (P) values were measured using Colorimetric Roche kits on a Roche/Hitachi fully automated biochemical analyzer. Data collection and analysis followed. Results: Higher levels of age, systolic and diastolic blood pressures were found in the traumatic fracture group compared to the control group, whereas weight, height, and blood phosphorus did not differ significantly (P adjusting for age, systolic blood pressure, diastolic blood pressure, and blood phosphorus, binary logistic regression analysis revealed that blood calcium was a protective factor against traumatic fracture (β = -26.85, OR = 0.00, 95% CI = 0.00 -0.02, P = 0.022). Conclusion: The findings suggest that high and low blood calcium levels may serve as useful indicators in predicting the prognosis of fractures resulting from trauma.展开更多
Objective To investigate gene expression of transforming growth factor-β(TGF-β)in local bony callus in tracumatic brain in jury combined with extremity long bone fracture in rats.Methods Eighty male SD rats were ran...Objective To investigate gene expression of transforming growth factor-β(TGF-β)in local bony callus in tracumatic brain in jury combined with extremity long bone fracture in rats.Methods Eighty male SD rats were randomized into 2 even展开更多
BACKGROUND Stroke is the leading cause of adult lifelong disability worldwide.A stroke is an acute cerebrovascular disease with a variety of causes and corresponding clinical symptoms.Around 75%of surviving stroke pat...BACKGROUND Stroke is the leading cause of adult lifelong disability worldwide.A stroke is an acute cerebrovascular disease with a variety of causes and corresponding clinical symptoms.Around 75%of surviving stroke patients experience impaired nerve function,and some suffer from traumatic fractures,which can lead to special care needs.AIM To determine the effect of timing theory continuous care,with resistance training,on the rehabilitation and mental health of caregivers and stroke patients with traumatic fractures.METHODS Between January 2017 to March 2021,we selected 100 hospital admissions with post-stroke hemiplegia complicated with a traumatic fracture.Two participant groups were created:(1)Control group:given resistance training;and(2)Observation group:given timing theory continuous care combined with resistance training.The degree of satisfaction and differences in bone and phosphorus metabolism indexes between the two groups were compared.The self-perceived burden scale(SPBS)and caregiver burden questionnaire were used to evaluate the psychological health of patients and caregivers.The Harris hip function score,ability of daily living(ADL)scale,and global quality of life questionnaire(GQOL-74)were used to evaluate hip function,ability of daily living,and quality of life.RESULTS Data were collected prior to and after intervention.Alkaline phosphatase(ALP),osteocalcin,and vitamin D3 in the observation group and control group increased after intervention(P<0.05),and carboxy-terminal peptide of type I collagenβSpecial sequence(β-CTX)decreased(P<0.05).ALP and osteocalcin in the observation group were higher than in the control group(P<0.05).There was no significant difference inβ-CTX and vitamin D3 between the two groups(P>0.05).The SPBS score of the observation group was lower and the ADL score was higher than the control group.The burden score was lower and the Harris hip function and GQOL-74 scores were higher than that of the control group(P<0.05).The observation group’s satisfaction rating was 94.00%,which was higher than the rating from the control group(P<0.05).CONCLUSION Timing theory continuous nursing with resistance training can reduce hip dysfunction in stroke patients with a traumatic fracture and enhance quality of life and mental health of patients and caregivers.展开更多
Objective:To observe the effect of nuclear transcription factor- κB(NF- κB) on cerebral edema in rats with traumatic brain injury(TBI).Methods:Male SD rau with fluid percussion injury(FPI) were selected.After separa...Objective:To observe the effect of nuclear transcription factor- κB(NF- κB) on cerebral edema in rats with traumatic brain injury(TBI).Methods:Male SD rau with fluid percussion injury(FPI) were selected.After separation and culture,rats' astrocytes all suffered FPL The expression of NF-κB and the water content were detected at the animal and cellular levels,while the activity of NOX was evaluated at the cellular level.Results:According to the results,the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI.while NF-κB inhibitor BAY 11-7082 could significantly reduce the effect of TBI.1 and 3h after FPI of astrocytes,the activation of NFkB was incrcased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes.After the injury of astrocytes,the activity of NOX was also increased,while BAY11-7082 could reduce the activity of NOX.Conclusions:The results show that the activation of NF- κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.展开更多
BACKGROUND Computed tomography(CT)has become a routine preoperative examination for tibial plateau fractures(TPFs).Assessing the location of the fragment and intercondylar eminence fracture can provide clinicians with...BACKGROUND Computed tomography(CT)has become a routine preoperative examination for tibial plateau fractures(TPFs).Assessing the location of the fragment and intercondylar eminence fracture can provide clinicians with valuable information;however,the evaluation of traumatic meniscal lesion(TML)and arthroscopic management are controversial.AIM To predict TML by three-dimensional skeletal anatomy changes in unilateral TPF and bilateral TPF on preoperative thin layer CT.METHODS Acute fracture of tibial plateau patients undergoing arthroscopic surgery between December 2017 and December 2019 were included in this retrospective study.The type,zone,and location of TMLs were diagnosed based on the operation records and/or arthroscopic videos.Measurement of three-dimensional fracture morphology included the following:Frontal fragment width of plateau,sagittal fragment subsiding distance(FSD),sagittal fracture line distance,sagittal posterior tibial slope,and transversal area ratio of fragment area)on preoperative CT three-dimensional plane.The correlation of TML with skeletal values was calculated according to unicondylar TPFs and bicondylar TPFs.RESULTS A total of 67 patients were enrolled in this study,among which 30 patients had TMLs,lateral/medial(23/7).FSD was a particularly positive factor to predict TML,with odds ratio of 2.31(1.26-5.63).On sagittal view of CT,FSD degree of 8 mm and posterior tibial slope exceeding 11.74°implied enhanced risk of TML in bicondylar TPFs.On coronal view,once fragment width of plateau surpassed 3 cm,incidence of TML reached 100%.On transverse view,area ratio of fragment as enhanced risk of 5.5%and FSD>4.3 mm for predicting TML were observed in unicondylar TPFs.CONCLUSION TML can be predicted by different parameters on preoperative CT views according to unicondylar fractures and bicondylar TPFs.展开更多
In this study, our objective was to evaluate effects of leptin on fracture healing in rats. Seventy two male SpragueDawley(SD) rats were randomized into 3 groups. Standardized femoral fractures were created in all the...In this study, our objective was to evaluate effects of leptin on fracture healing in rats. Seventy two male SpragueDawley(SD) rats were randomized into 3 groups. Standardized femoral fractures were created in all the rats. Group A was treated with 1 mL normal saline(NS), group B with 0.3μg/kg leptin in 1 mL NS, and group C with 0.5μg/kg leptin in 1 mL NS for 2 weeks intraperitoneally. Each group was divided into three subgroups including 8 rats for evaluation at 2, 4 and 8 weeks. Radiological evaluation showed that callus formation of group B and C was all significantly higher than group A at 8 weeks(P=0.04 and P = 0.013, respectively). There was no statistically significant difference in fracture healing between group B and group C at 8 weeks(P = 0.197). Histological evaluation revealed fracture healing of group B and C was better than group A at 4 weeks(P=0.01 and P= 0.002,respectively) and 8 weeks(P = 0.008 and P= 0.003, respectively). Micro-computed tomography(Micro-CT) analysis demonstrated that greater amounts of bony callus and evidence of bone fusion were observed in group B and C at 4 weeks(P = 0.02 and P = 0.04, respectively) and 8 weeks(P= 0.005 and P = 0.001, respectively) compared to group A. Group C also had better fracture healing than group B at 8 weeks(P = 0.01). In conclusion, leptin has a positive effect on rat femoral fracture healing.展开更多
BACKGROUND In general,atlantoaxial dislocation is rare due to the stability of the C1-C2 complex.Traumatic atlantoaxial dislocations are usually anterior and accompanied by odontoid fractures.Posterior atlantoaxial di...BACKGROUND In general,atlantoaxial dislocation is rare due to the stability of the C1-C2 complex.Traumatic atlantoaxial dislocations are usually anterior and accompanied by odontoid fractures.Posterior atlantoaxial dislocations are rare,and complete posterior dislocation without associated fracture is even more rare.A case of early recurrence of posterior atlantoaxial dislocation without fracture being in therapy of first closed reduction and then open reduction has not been previously reported.CASE SUMMARY A 45-year-old female presented with traumatic posterior atlantoaxial dislocation(TPAD)of C1-C2 without associated fractures,and Frankel Grade B spinal cord function.She was successfully managed by immediate closed reduction under skull traction.Unexpectedly,17 d later,re-dislocation was discovered.On day 28,closed reduction was performed as before but failed.Then,open reduction and posterior internal fixation with autologous iliac bone grafts was performed.By 6 mo after surgery,atlantoaxial joint fusion was achieved,and neurological function had recovered to Frankel Grade E.At 12 mo follow-up,she had lost only 15°of cervical rotation,and atlantoaxial complex instability in joint flexing and extending were no longer observed under fluoroscopy.CONCLUSION Early assessment of transverse ligament is critical for TPAD without fracture avoiding re-dislocation after closed reduction.展开更多
Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wist...Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.展开更多
BACKGROUND: The effects of N-methyl-D-aspartic acid (NMDA) receptor antagonist on neurodegeneration in the immature brain following traumatic brain injury (TBI) are still widely unknown. OBJECTIVE: To study the effect...BACKGROUND: The effects of N-methyl-D-aspartic acid (NMDA) receptor antagonist on neurodegeneration in the immature brain following traumatic brain injury (TBI) are still widely unknown. OBJECTIVE: To study the effects of dizocipine maleate (MK-801),a non-competitive NMDA receptor antagonist,on mitochondrial ultramicrostructure of neurons in the ipsilateral cingulate cortex and hippocampus after TBI in neonatal rats,and to analyze the optimal time interval of MK-801 administration (1 mg/kg). DESIGN: Completely randomized controlled study. SETTING: Shanghai Jiao Tong University. MATERIALS: Eight 7-day-old neonatal SD rats,irrespective of gender,were provided by Experimental Animal Center,Medical College of Fudan University. The experiment was approved by a local ethics committee. MK-801 was provided by Sigma. A CM-120 transmission electron microscope (Philips,Holland) was used for tissue analysis. METHODS: This study was performed at the Departments of Anatomy,Neuromorphology,and Biophysics,Medical College of Shanghai,Jiaotong University,between October 2006 and January 2007. Focal models of contusion and laceration of brain were established by the free-falling impact method. Eight rats were randomly divided into a normal control group (n = 2 ) and a MK-801 group (n = 6). Rats in the normal control group did not receive model establishment and administration,and they were only analyzed by an electron microscope. In the MK-801 group,the cingulate cortex was damaged using a contusion device. MK-801 (1 mg/kg) was intraperitoneally injected 30 minutes before lesion,immediately after lesion,and 30 minutes after lesion (n = 2 for each time point). MAIN OUTCOME MEASURES: The cingulate cortex and hippocampal tissues from the injured side were removed 24 hours after lesion and routinely processed for analysis of neuronal ultramicrostructure using transmission electron microscopy. RESULTS: Differential therapeutic effects of MK-801 (1 mg/kg) at distinct administration time points: thirty minutes before lesion,the shape of cortical and hippocampal neurons was similar to that observed during excitotoxicity-induced cell death. Organelles were enlarged,the nuclear membrane of cortical neurons was complete with gear wheel-like changes,and the nuclear chromatin was irregularly aggregated around the edge. When MK-801 was applied 30 minutes after lesion,the cingulate cortex contained apoptotic neurons in early and late stages. The nuclear membrane of hippocampal neurons displayed incisures. The chromatin shape was not similar to necrosis in an early stage. Immediate administration of MK-801 after lesion slightly altered the neuronal architecture,such that mitochondria were enlarged. The neuronal shape in the control group was normal. Effects of immediate administration of MK-801 on mitochondrial injury following TBI were that the mitochondria in cortical and hippocampal neurons were damaged to a certain degree in the MK-801 group. Mitochondrial injury was reversible,when MK-801 was applied 30 minutes before lesion and immediately after lesion. Application 30 minutes after lesion produced irreversible changes. In addition,mitochondrial injury occurred earlier than other organelle and nuclear changes. CONCLUSION: Mitochondrial injury occurs earlier than other organelle and nuclear changes. Early administration of MK-801 (1 mg/kg) can prevent or reduce necrosis following TBI,decrease the degree of neuronal injury,and protect nerve cells.展开更多
Objective:To explore the effect of depression on fracture healingand forecast its agnostic mechanism.Methods:Rats(Sprague Dawley,female,8-month-old,(242.6±11.2)g)wererandomly divided into Control(group A),Sham Co...Objective:To explore the effect of depression on fracture healingand forecast its agnostic mechanism.Methods:Rats(Sprague Dawley,female,8-month-old,(242.6±11.2)g)wererandomly divided into Control(group A),Sham Control(group B)and Depression(group C)groups.Upper 1/3 transverse tibia fracture model and intramedullaryfix were applied to Group A and C and sham operations(similar surgeries with no fracture and fix)were applied to Group B.The serum calcium(Ca),phosphorus(P)and alkaline phosphatase(ALP)were measured on day 7,14,and 21 after operation;the tibia image(DR)and pathology(HE stain)were examined onday14,28,and 56 after operation;the Biomechanics(three-point bending test)were implemented on day 35 and 56 after operation;and the Micro-CT were performed on day 28 and 56 after operation.Results:The tibia image(DR)showed no significant difference in callus volume between Groups A and C on Day 14 after operation(P>0.05,n=10),but had significant difference on Day 28 and 56 after operation(P<0.05,n=10).HE staining showed significantly worse recovery of the fracture zone in Group C than in Group A on Day 14,28,and 56 after operation.The results from three point bending experiment revealed a significantly worse recovery of all bending indices(stiffness,strength,deflection,load,and movement)of Group C than the control Group A on Day 35 and 56 after operation(P<0.05,n=10).Micro-CTalsoshowed that the osteogenesis indicators such as bone volume fraction,thickness of trabecular bone,and number of trabecular bone were significantly lower in Group C than that in the control group A on Day 28 and 56 after operation(P<0.05,n=10).The volume of interstitial bone trabecular space and area of trabecular bone of Group C was significantly greater than that of control Group A(P<0.05,n=10).The serum P and ALP concentrations of Group C were significantly lower than those of group Bon Day 7(P<0.05,P<0.01,n=10),14(P<0.05,P<0.01,n=10),and 21(P<0.05,P<0.01,n=10)after operation,while the serum Ca concentrations did not show any signifi cant changes(P>0.05,n=10).Conclusion:Depression may delay the primary callus and tibia fracture healing through an effect on the metabolism of phosphorus which caused a down-regulation of serum P and ALP concentrations in rats.These results provide a novel underlying mechanism and also a potential therapeutic strategy for depression caused delay of bone fracture healing.展开更多
Objective To explore the relevant risk factors of tramatic arthritis resulting from the surgery of acetabular fractures. Methods From January 2000 to January 2009,88 patients aged from 20 to 60 years old with acetabul...Objective To explore the relevant risk factors of tramatic arthritis resulting from the surgery of acetabular fractures. Methods From January 2000 to January 2009,88 patients aged from 20 to 60 years old with acetabular fractures展开更多
目的:深入研究全髋关节置换术(total hip arthroplasty,THA)治疗髋臼骨折继发创伤性关节炎的临床疗效。方法:回顾性分析2019年10月至2022年6月,采用THA治疗15例髋臼骨折继发创伤性关节炎患者,男8例,女7例,年龄40~76(59.20±9.46)岁...目的:深入研究全髋关节置换术(total hip arthroplasty,THA)治疗髋臼骨折继发创伤性关节炎的临床疗效。方法:回顾性分析2019年10月至2022年6月,采用THA治疗15例髋臼骨折继发创伤性关节炎患者,男8例,女7例,年龄40~76(59.20±9.46)岁。记录术前及术后假体松动、髋关节脱位、髋关节活动度、神经损伤等情况,采用Harris评分、视觉模拟评分(visual analogue scale,VAS)、影像学检查评价髋关节功能及手术疗效。结果:15例均顺利完成手术,术中无神经、血管损伤,术后切口均Ⅰ期甲级愈合,无感染。随访时间6~39(18.33±9.27)个月。其中1例术后半年发生髋臼侧假体松动,经过翻修手术后恢复良好;1例髋关节脱位,经切开复位处理后治愈,无不良后果。术后末次随访Harris评分(88.60±4.01)分,与术前(47.20±11.77)分相比,差异有统计学意义(P<0.05)。术后末次随访VAS评分1(1)分,与术前8(2)分相比,差异有统计学意义(P<0.05)。末次随访时,本组所有患者疼痛症状减轻或消失,关节功能满意;末次随访影像学资料显示关节假体贴合良好,无异位骨化发生,假体无松动。结论:THA治疗髋臼骨折继发创伤性关节炎疗效可靠,能有效提高患者的生活质量。术前对患者全面评估、骨缺损评估,术中髋臼、股骨、内固定物与骨缺损的处理是手术成功的关键因素。展开更多
文摘Purpose: This study aims to investigate the predictive value of blood calcium in the prognosis of traumatic fracture. Methods: A retrospective experimental design was employed, 112 cases (52 non-fracture and 60 traumatic fracture) were randomly selected. The type of fracture complies with WHO-recommended (2019) diagnostic criteria for osteoporosis combined with fracture. The blood pressure (BP) was measured by OMRON’s HEM-7136 model electronic blood pressure monitor. Blood calcium (Ca<sup>2+</sup>), and blood phosphorus (P) values were measured using Colorimetric Roche kits on a Roche/Hitachi fully automated biochemical analyzer. Data collection and analysis followed. Results: Higher levels of age, systolic and diastolic blood pressures were found in the traumatic fracture group compared to the control group, whereas weight, height, and blood phosphorus did not differ significantly (P adjusting for age, systolic blood pressure, diastolic blood pressure, and blood phosphorus, binary logistic regression analysis revealed that blood calcium was a protective factor against traumatic fracture (β = -26.85, OR = 0.00, 95% CI = 0.00 -0.02, P = 0.022). Conclusion: The findings suggest that high and low blood calcium levels may serve as useful indicators in predicting the prognosis of fractures resulting from trauma.
文摘Objective To investigate gene expression of transforming growth factor-β(TGF-β)in local bony callus in tracumatic brain in jury combined with extremity long bone fracture in rats.Methods Eighty male SD rats were randomized into 2 even
文摘BACKGROUND Stroke is the leading cause of adult lifelong disability worldwide.A stroke is an acute cerebrovascular disease with a variety of causes and corresponding clinical symptoms.Around 75%of surviving stroke patients experience impaired nerve function,and some suffer from traumatic fractures,which can lead to special care needs.AIM To determine the effect of timing theory continuous care,with resistance training,on the rehabilitation and mental health of caregivers and stroke patients with traumatic fractures.METHODS Between January 2017 to March 2021,we selected 100 hospital admissions with post-stroke hemiplegia complicated with a traumatic fracture.Two participant groups were created:(1)Control group:given resistance training;and(2)Observation group:given timing theory continuous care combined with resistance training.The degree of satisfaction and differences in bone and phosphorus metabolism indexes between the two groups were compared.The self-perceived burden scale(SPBS)and caregiver burden questionnaire were used to evaluate the psychological health of patients and caregivers.The Harris hip function score,ability of daily living(ADL)scale,and global quality of life questionnaire(GQOL-74)were used to evaluate hip function,ability of daily living,and quality of life.RESULTS Data were collected prior to and after intervention.Alkaline phosphatase(ALP),osteocalcin,and vitamin D3 in the observation group and control group increased after intervention(P<0.05),and carboxy-terminal peptide of type I collagenβSpecial sequence(β-CTX)decreased(P<0.05).ALP and osteocalcin in the observation group were higher than in the control group(P<0.05).There was no significant difference inβ-CTX and vitamin D3 between the two groups(P>0.05).The SPBS score of the observation group was lower and the ADL score was higher than the control group.The burden score was lower and the Harris hip function and GQOL-74 scores were higher than that of the control group(P<0.05).The observation group’s satisfaction rating was 94.00%,which was higher than the rating from the control group(P<0.05).CONCLUSION Timing theory continuous nursing with resistance training can reduce hip dysfunction in stroke patients with a traumatic fracture and enhance quality of life and mental health of patients and caregivers.
基金supported by Technology Program for Development of Pharmacy of Shandong 2013(No.2013WS0351)
文摘Objective:To observe the effect of nuclear transcription factor- κB(NF- κB) on cerebral edema in rats with traumatic brain injury(TBI).Methods:Male SD rau with fluid percussion injury(FPI) were selected.After separation and culture,rats' astrocytes all suffered FPL The expression of NF-κB and the water content were detected at the animal and cellular levels,while the activity of NOX was evaluated at the cellular level.Results:According to the results,the positive expression of NF-κB and expression of mRNA were significantly increased and the water content was increased for rats after TBI.while NF-κB inhibitor BAY 11-7082 could significantly reduce the effect of TBI.1 and 3h after FPI of astrocytes,the activation of NFkB was incrcased and BAY 11-7082 could significantly improve the injury-induced swelling of astrocytes.After the injury of astrocytes,the activity of NOX was also increased,while BAY11-7082 could reduce the activity of NOX.Conclusions:The results show that the activation of NF- κB in astrocytes is a key factor in the process of cerebral edema after TBI of rats.
基金The Jiangmen Science and Technology Project,No.2017A2018.
文摘BACKGROUND Computed tomography(CT)has become a routine preoperative examination for tibial plateau fractures(TPFs).Assessing the location of the fragment and intercondylar eminence fracture can provide clinicians with valuable information;however,the evaluation of traumatic meniscal lesion(TML)and arthroscopic management are controversial.AIM To predict TML by three-dimensional skeletal anatomy changes in unilateral TPF and bilateral TPF on preoperative thin layer CT.METHODS Acute fracture of tibial plateau patients undergoing arthroscopic surgery between December 2017 and December 2019 were included in this retrospective study.The type,zone,and location of TMLs were diagnosed based on the operation records and/or arthroscopic videos.Measurement of three-dimensional fracture morphology included the following:Frontal fragment width of plateau,sagittal fragment subsiding distance(FSD),sagittal fracture line distance,sagittal posterior tibial slope,and transversal area ratio of fragment area)on preoperative CT three-dimensional plane.The correlation of TML with skeletal values was calculated according to unicondylar TPFs and bicondylar TPFs.RESULTS A total of 67 patients were enrolled in this study,among which 30 patients had TMLs,lateral/medial(23/7).FSD was a particularly positive factor to predict TML,with odds ratio of 2.31(1.26-5.63).On sagittal view of CT,FSD degree of 8 mm and posterior tibial slope exceeding 11.74°implied enhanced risk of TML in bicondylar TPFs.On coronal view,once fragment width of plateau surpassed 3 cm,incidence of TML reached 100%.On transverse view,area ratio of fragment as enhanced risk of 5.5%and FSD>4.3 mm for predicting TML were observed in unicondylar TPFs.CONCLUSION TML can be predicted by different parameters on preoperative CT views according to unicondylar fractures and bicondylar TPFs.
文摘In this study, our objective was to evaluate effects of leptin on fracture healing in rats. Seventy two male SpragueDawley(SD) rats were randomized into 3 groups. Standardized femoral fractures were created in all the rats. Group A was treated with 1 mL normal saline(NS), group B with 0.3μg/kg leptin in 1 mL NS, and group C with 0.5μg/kg leptin in 1 mL NS for 2 weeks intraperitoneally. Each group was divided into three subgroups including 8 rats for evaluation at 2, 4 and 8 weeks. Radiological evaluation showed that callus formation of group B and C was all significantly higher than group A at 8 weeks(P=0.04 and P = 0.013, respectively). There was no statistically significant difference in fracture healing between group B and group C at 8 weeks(P = 0.197). Histological evaluation revealed fracture healing of group B and C was better than group A at 4 weeks(P=0.01 and P= 0.002,respectively) and 8 weeks(P = 0.008 and P= 0.003, respectively). Micro-computed tomography(Micro-CT) analysis demonstrated that greater amounts of bony callus and evidence of bone fusion were observed in group B and C at 4 weeks(P = 0.02 and P = 0.04, respectively) and 8 weeks(P= 0.005 and P = 0.001, respectively) compared to group A. Group C also had better fracture healing than group B at 8 weeks(P = 0.01). In conclusion, leptin has a positive effect on rat femoral fracture healing.
文摘BACKGROUND In general,atlantoaxial dislocation is rare due to the stability of the C1-C2 complex.Traumatic atlantoaxial dislocations are usually anterior and accompanied by odontoid fractures.Posterior atlantoaxial dislocations are rare,and complete posterior dislocation without associated fracture is even more rare.A case of early recurrence of posterior atlantoaxial dislocation without fracture being in therapy of first closed reduction and then open reduction has not been previously reported.CASE SUMMARY A 45-year-old female presented with traumatic posterior atlantoaxial dislocation(TPAD)of C1-C2 without associated fractures,and Frankel Grade B spinal cord function.She was successfully managed by immediate closed reduction under skull traction.Unexpectedly,17 d later,re-dislocation was discovered.On day 28,closed reduction was performed as before but failed.Then,open reduction and posterior internal fixation with autologous iliac bone grafts was performed.By 6 mo after surgery,atlantoaxial joint fusion was achieved,and neurological function had recovered to Frankel Grade E.At 12 mo follow-up,she had lost only 15°of cervical rotation,and atlantoaxial complex instability in joint flexing and extending were no longer observed under fluoroscopy.CONCLUSION Early assessment of transverse ligament is critical for TPAD without fracture avoiding re-dislocation after closed reduction.
基金National Natural Science Foundation of China(No.81904258)。
文摘Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.
基金the Fourth Key Disciplines Foundation of Shanghai Education Commission, No.2004JY04
文摘BACKGROUND: The effects of N-methyl-D-aspartic acid (NMDA) receptor antagonist on neurodegeneration in the immature brain following traumatic brain injury (TBI) are still widely unknown. OBJECTIVE: To study the effects of dizocipine maleate (MK-801),a non-competitive NMDA receptor antagonist,on mitochondrial ultramicrostructure of neurons in the ipsilateral cingulate cortex and hippocampus after TBI in neonatal rats,and to analyze the optimal time interval of MK-801 administration (1 mg/kg). DESIGN: Completely randomized controlled study. SETTING: Shanghai Jiao Tong University. MATERIALS: Eight 7-day-old neonatal SD rats,irrespective of gender,were provided by Experimental Animal Center,Medical College of Fudan University. The experiment was approved by a local ethics committee. MK-801 was provided by Sigma. A CM-120 transmission electron microscope (Philips,Holland) was used for tissue analysis. METHODS: This study was performed at the Departments of Anatomy,Neuromorphology,and Biophysics,Medical College of Shanghai,Jiaotong University,between October 2006 and January 2007. Focal models of contusion and laceration of brain were established by the free-falling impact method. Eight rats were randomly divided into a normal control group (n = 2 ) and a MK-801 group (n = 6). Rats in the normal control group did not receive model establishment and administration,and they were only analyzed by an electron microscope. In the MK-801 group,the cingulate cortex was damaged using a contusion device. MK-801 (1 mg/kg) was intraperitoneally injected 30 minutes before lesion,immediately after lesion,and 30 minutes after lesion (n = 2 for each time point). MAIN OUTCOME MEASURES: The cingulate cortex and hippocampal tissues from the injured side were removed 24 hours after lesion and routinely processed for analysis of neuronal ultramicrostructure using transmission electron microscopy. RESULTS: Differential therapeutic effects of MK-801 (1 mg/kg) at distinct administration time points: thirty minutes before lesion,the shape of cortical and hippocampal neurons was similar to that observed during excitotoxicity-induced cell death. Organelles were enlarged,the nuclear membrane of cortical neurons was complete with gear wheel-like changes,and the nuclear chromatin was irregularly aggregated around the edge. When MK-801 was applied 30 minutes after lesion,the cingulate cortex contained apoptotic neurons in early and late stages. The nuclear membrane of hippocampal neurons displayed incisures. The chromatin shape was not similar to necrosis in an early stage. Immediate administration of MK-801 after lesion slightly altered the neuronal architecture,such that mitochondria were enlarged. The neuronal shape in the control group was normal. Effects of immediate administration of MK-801 on mitochondrial injury following TBI were that the mitochondria in cortical and hippocampal neurons were damaged to a certain degree in the MK-801 group. Mitochondrial injury was reversible,when MK-801 was applied 30 minutes before lesion and immediately after lesion. Application 30 minutes after lesion produced irreversible changes. In addition,mitochondrial injury occurred earlier than other organelle and nuclear changes. CONCLUSION: Mitochondrial injury occurs earlier than other organelle and nuclear changes. Early administration of MK-801 (1 mg/kg) can prevent or reduce necrosis following TBI,decrease the degree of neuronal injury,and protect nerve cells.
文摘Objective:To explore the effect of depression on fracture healingand forecast its agnostic mechanism.Methods:Rats(Sprague Dawley,female,8-month-old,(242.6±11.2)g)wererandomly divided into Control(group A),Sham Control(group B)and Depression(group C)groups.Upper 1/3 transverse tibia fracture model and intramedullaryfix were applied to Group A and C and sham operations(similar surgeries with no fracture and fix)were applied to Group B.The serum calcium(Ca),phosphorus(P)and alkaline phosphatase(ALP)were measured on day 7,14,and 21 after operation;the tibia image(DR)and pathology(HE stain)were examined onday14,28,and 56 after operation;the Biomechanics(three-point bending test)were implemented on day 35 and 56 after operation;and the Micro-CT were performed on day 28 and 56 after operation.Results:The tibia image(DR)showed no significant difference in callus volume between Groups A and C on Day 14 after operation(P>0.05,n=10),but had significant difference on Day 28 and 56 after operation(P<0.05,n=10).HE staining showed significantly worse recovery of the fracture zone in Group C than in Group A on Day 14,28,and 56 after operation.The results from three point bending experiment revealed a significantly worse recovery of all bending indices(stiffness,strength,deflection,load,and movement)of Group C than the control Group A on Day 35 and 56 after operation(P<0.05,n=10).Micro-CTalsoshowed that the osteogenesis indicators such as bone volume fraction,thickness of trabecular bone,and number of trabecular bone were significantly lower in Group C than that in the control group A on Day 28 and 56 after operation(P<0.05,n=10).The volume of interstitial bone trabecular space and area of trabecular bone of Group C was significantly greater than that of control Group A(P<0.05,n=10).The serum P and ALP concentrations of Group C were significantly lower than those of group Bon Day 7(P<0.05,P<0.01,n=10),14(P<0.05,P<0.01,n=10),and 21(P<0.05,P<0.01,n=10)after operation,while the serum Ca concentrations did not show any signifi cant changes(P>0.05,n=10).Conclusion:Depression may delay the primary callus and tibia fracture healing through an effect on the metabolism of phosphorus which caused a down-regulation of serum P and ALP concentrations in rats.These results provide a novel underlying mechanism and also a potential therapeutic strategy for depression caused delay of bone fracture healing.
文摘Objective To explore the relevant risk factors of tramatic arthritis resulting from the surgery of acetabular fractures. Methods From January 2000 to January 2009,88 patients aged from 20 to 60 years old with acetabular fractures
文摘目的:深入研究全髋关节置换术(total hip arthroplasty,THA)治疗髋臼骨折继发创伤性关节炎的临床疗效。方法:回顾性分析2019年10月至2022年6月,采用THA治疗15例髋臼骨折继发创伤性关节炎患者,男8例,女7例,年龄40~76(59.20±9.46)岁。记录术前及术后假体松动、髋关节脱位、髋关节活动度、神经损伤等情况,采用Harris评分、视觉模拟评分(visual analogue scale,VAS)、影像学检查评价髋关节功能及手术疗效。结果:15例均顺利完成手术,术中无神经、血管损伤,术后切口均Ⅰ期甲级愈合,无感染。随访时间6~39(18.33±9.27)个月。其中1例术后半年发生髋臼侧假体松动,经过翻修手术后恢复良好;1例髋关节脱位,经切开复位处理后治愈,无不良后果。术后末次随访Harris评分(88.60±4.01)分,与术前(47.20±11.77)分相比,差异有统计学意义(P<0.05)。术后末次随访VAS评分1(1)分,与术前8(2)分相比,差异有统计学意义(P<0.05)。末次随访时,本组所有患者疼痛症状减轻或消失,关节功能满意;末次随访影像学资料显示关节假体贴合良好,无异位骨化发生,假体无松动。结论:THA治疗髋臼骨折继发创伤性关节炎疗效可靠,能有效提高患者的生活质量。术前对患者全面评估、骨缺损评估,术中髋臼、股骨、内固定物与骨缺损的处理是手术成功的关键因素。
