Objective- To compare the consistency of the results from detecting HIV-1 antibody in the paired urine and serum specimens from drug users by ELISA. Methods: The paired urine and serum specimens from 273 drug users de...Objective- To compare the consistency of the results from detecting HIV-1 antibody in the paired urine and serum specimens from drug users by ELISA. Methods: The paired urine and serum specimens from 273 drug users detained at a detoxification unit were collected, and the HIV-1 antibodies in the specimens of them were screened by urine and serum ELISA kits, respectively. Results: Of 273 serum specimens, 94 ones showed positive reaction and among 94 counterpart urine specimens, 93 ones also appeared positive reaction. Taking the results together,the consistent rate of HIV-1 antibody screened by urine and serum ELISA kits was 99.6%. Conclusion: The urine ELISA kit, which screened HIV-1 antibody of urine showing almost the same results tested by serum ELISA kit, is reliable. It is proposed that urine ELISA be introduced in many fields.展开更多
Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher r...Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher rate of voiding within 5 minutes using this method.We evaluated this method in an Irish hospital providing secondary care.Methods A non-blinded,randomized,controlled trial was carried out.Eligible infants were between 1 and 12 months of age,who required urine sampling as part of clinical care.Participants were randomly allocated to receive the intervention(Quick Wee Method—supra-pubic stimulation with cold saline)or the control(usual care—clean catch with no bladder stimulation)for 5 min.Primary outcome was voiding of urine within 5 min.Results A total of 140 infants were included in this study(73 in intervention group;67 in control group).Baseline characteristics were similar.25%in the intervention group passed urine in the 5-min trial period compared with 18%in the control group[P=0.4,absolute diference 7%(95%confdence interval:−7%to+20%)].Conclusion The Quick Wee method is a simple and inexpensive intervention that did not show a statistically signifcant increase in urine samples obtained in pre-continent infants.展开更多
文摘Objective- To compare the consistency of the results from detecting HIV-1 antibody in the paired urine and serum specimens from drug users by ELISA. Methods: The paired urine and serum specimens from 273 drug users detained at a detoxification unit were collected, and the HIV-1 antibodies in the specimens of them were screened by urine and serum ELISA kits, respectively. Results: Of 273 serum specimens, 94 ones showed positive reaction and among 94 counterpart urine specimens, 93 ones also appeared positive reaction. Taking the results together,the consistent rate of HIV-1 antibody screened by urine and serum ELISA kits was 99.6%. Conclusion: The urine ELISA kit, which screened HIV-1 antibody of urine showing almost the same results tested by serum ELISA kit, is reliable. It is proposed that urine ELISA be introduced in many fields.
文摘Introduction The acquisition of non-contaminated urine samples in pre-continent infants remains a challenge.The Quick Wee method uses bladder stimulation to induce voiding.A previous randomized trial showed a higher rate of voiding within 5 minutes using this method.We evaluated this method in an Irish hospital providing secondary care.Methods A non-blinded,randomized,controlled trial was carried out.Eligible infants were between 1 and 12 months of age,who required urine sampling as part of clinical care.Participants were randomly allocated to receive the intervention(Quick Wee Method—supra-pubic stimulation with cold saline)or the control(usual care—clean catch with no bladder stimulation)for 5 min.Primary outcome was voiding of urine within 5 min.Results A total of 140 infants were included in this study(73 in intervention group;67 in control group).Baseline characteristics were similar.25%in the intervention group passed urine in the 5-min trial period compared with 18%in the control group[P=0.4,absolute diference 7%(95%confdence interval:−7%to+20%)].Conclusion The Quick Wee method is a simple and inexpensive intervention that did not show a statistically signifcant increase in urine samples obtained in pre-continent infants.
