Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To pr...Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization , we examined mRNA populations in differently_treated plumules of winter wheat \%(Triticum aestivum \%L. \%cv\% Yanda 1817) using mRNA differential display. One vernalization_ related cDNA clone \%(VRC), VRC54\%, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that \%VRC54\% was a novel vernalization_related clone found in higher plant which not only might play an important role in the floral induction in vernalization_requiring plants but also was different from the cold_acclimatized genes.展开更多
文摘Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization , we examined mRNA populations in differently_treated plumules of winter wheat \%(Triticum aestivum \%L. \%cv\% Yanda 1817) using mRNA differential display. One vernalization_ related cDNA clone \%(VRC), VRC54\%, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that \%VRC54\% was a novel vernalization_related clone found in higher plant which not only might play an important role in the floral induction in vernalization_requiring plants but also was different from the cold_acclimatized genes.
