Background:Exploring the efficacy,potential components,and mechanism of the combination of ginger essential oil and gingerols in the treatment of head wind disease based on network pharmacology technology with content...Background:Exploring the efficacy,potential components,and mechanism of the combination of ginger essential oil and gingerols in the treatment of head wind disease based on network pharmacology technology with content weight.Methods:The experimental groups were divided into:0:10,1:4,1:2,1:1,2:1,4:1,10:0.The relative content(Ri)of the chemical constituents of ginger's volatile oil was determined using gas chromatography-mass spectrometry(GC-MS).Additionally,the physicochemical and biological property parameters(LogP,MDCK,PPB,MW)of the components were considered.To assess the quantitative effect of the components,a grading score was performed,and the quantitative effect index(Ki)was calculated.Subsequently,the target effect index(Ti)was calculated by combining the component-target matching score(Fit score).Using these calculations,the target effect score A was determined under the influence of multiple components targeting different targets.Key targets with A≥1000 were identified.To predict the targets related to head wind disease,the Comparative Toxicogenomics Database(https://ctdbase.org/),Gene Cards(https://www.genecards.org/),and Disgenet database(https://www.disgenet.org/)were utilized.The key targets,obtained from different proportions of ginger's volatile oil and gingerol,were intersected with the predicted targets.This facilitated network pharmacological analysis and verification of the efficacy.Results:The content of volatile oil in ginger demonstrated an impact on key targets associated with the volatile oil group.Each specific combination of volatile oil consistently activated distinct pathways,with variations stemming from changes in content.Experimental testing revealed that different combinations of ginger's volatile oil and gingerol effectively alleviated migraine symptoms in rats.Conclusion:Through the application of content-weighted network pharmacology technology and pharmacodynamic verification,it was determined that altering the ratio between ginger's volatile oil and gingerol leads to variations in potential targets and pathways,consequently impacting its efficacy.展开更多
BZ26-6 Oilfield is a kind of deep metamorphic rock buried-hill volatile oilfield in Bohai Sea, China. Its early development plan is restricted due to the simultaneous production of oil and gas in large sections of res...BZ26-6 Oilfield is a kind of deep metamorphic rock buried-hill volatile oilfield in Bohai Sea, China. Its early development plan is restricted due to the simultaneous production of oil and gas in large sections of reservoirs, unclear understanding of formation fluid properties and uncertainty of gas-oil interface. Through theoretical research on phase recovery and experimental analysis of crude oil phase characteristics in the original formation, characteristic parameters of the equilibrium condensate gas fluid are restored and calculated. Through the superimposed phase diagram of volatile oil and condensate gas, BZ26-6 Oilfield is determined to be a volatile oil reservoir with a condensate gas cap, with formation pressure and saturation pressure of 36.1 MPa, respectively. Based on the research results of oil-gas phase behavior characteristics, the thermodynamic equations and equation of state are jointly used to solve the problem, and the content change curves of each component at different depths are drawn. Combined with the sensitivity analysis of numerical simulation, the gas-oil interface is determined to be -3726 m above sea level. The fluid phase analysis software, Fluidmodeler, is used to simulate volatile oil degassing and condensate gas separation experiments. In combination with oil and gas production data obtained through the production test, the specific oil recovery index and the specific gas recovery index are determined to be 0.408 m<sup>3</sup>/(MPa·d·m) and 1195 m<sup>3</sup>/(MPa·d·m), respectively. And the reasonable production capacity prediction is conducted on the early development of BZ26-6 Oilfield. The research results can provide a theoretical basis for the efficient development of similar complex oil and gas reservoirs.展开更多
This study was designed to investigate the anti-inflammatory effects of volatile oil of Platycladus orientalis(L.)Franco leaves(VOPF)and the underlying molecular mechanisms by using the non-infectious inflammation rat...This study was designed to investigate the anti-inflammatory effects of volatile oil of Platycladus orientalis(L.)Franco leaves(VOPF)and the underlying molecular mechanisms by using the non-infectious inflammation rat models and infectious inflammation mouse models.Ear swelling and intraperitoneal capillary permeability in mice,and carrageenan-induced toe swelling and cotton ball-induced granuloma in rats were used to reveal anti-inflammatory effects of VOPF.Moreover,the lipopolysaccharide(LPS)-induced mouse model of acute lung injury was used to explore the anti-inflammatory mechanism of VOPF.The results showed that VOPF could significantly inhibit auricular swelling,intraperitoneal capillary permeability in mice,and reduce granuloma swelling and paw swelling in rats.Furthermore,it significantly alleviated the pathological damage of the lung tissue.In addition,VOPF could reduce the contents of IL-1β and TNF-αand increase the content of IL-10 in the serum.It had little effect on the expression of p65 but reduced the phosphorylation level of p65 and IκB in NF-κB pathway.In conclusion,VOPF has anti-inflammatory effects and the mechanisms involve the down-regulation of the phosphorylation levels of p65 and IκB and blockage of the NF-κB signaling pathway.展开更多
[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-M...[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-MS method combined with retention index and peak area normalization method was used for qualitative and quantitative analysis.[Results]The volatile oils of mango leaves in southern provinces contained high content of terpenes,such asα-gurjunene(18.39%-34.13%),(+)-viridiflorene(8.95%-16.30%)and(E)-β-ocimene(3.49%-7.63%).The volatile oils components of mango leaves were significantly different in different provinces,for example,mango leaves produced in Hainan Province contained a large amount ofβ-selinene(23.00%),but not in other provinces.[Conclusions]The origin had a great influence on the composition and contents of volatile oils in mango leaves.展开更多
Rosa x alba is cultivated almost exclusively for the production of aromatic water and fruits widely used as ingredients in some traditional food preparations sold commercially.Results of the proximate and elemental an...Rosa x alba is cultivated almost exclusively for the production of aromatic water and fruits widely used as ingredients in some traditional food preparations sold commercially.Results of the proximate and elemental analyses revealed that flowers exhibited higher moisture content while the dry matter content was higher in leaves.Total fat and ash was higher in fruits when compared with leaves and flowers.All studied organs were found to be rich in essential mineral such as K,Ca,P and Mg.Qualitative and quantitative analysis of the volatile oils revealed different composition patterns between organs.Linalool and geraniol were found as major constituents of the leaf oil whereas 2-phenethyl alcohol and eugenol were the major constituents of the floral oil.In contrast,fruits showed very distinct composition and alkanes/alkenes were found to have the major contribution.The present composition could justify the traditional use of Rosa x alba which could be considered as a potential source of essential minerals and volatile constituents.展开更多
[Objectives] To increase the reserves distribution and planting area of Ferula resources,protect wild Ferula resources,make comparative analysis on wild and cultivated varieties of Ferula medicinal materials,and provi...[Objectives] To increase the reserves distribution and planting area of Ferula resources,protect wild Ferula resources,make comparative analysis on wild and cultivated varieties of Ferula medicinal materials,and provide references for quality evaluation of Ferula herbs collected from cultivation environment. [Methods] Volatile oil determination and high performance liquid chromatography were used to measure volatile oil and ferulic acid in different parts of wild and cultivated varieties of Ferula. [Results] In the volatile oil measured from artificially pressed Ferula lipid,wild varieties in different places conformed to the pharmacopoeia standard( volatile oil content ≥10%); in cultivated varieties,F. fukanensis K. M. Shen and F. sinkiangensis K. M. shen reached the pharmacopoeia standard. According to measurement results of ferulic acid,the ferulic acid content of roots and leaves of Altay,Fukang,and Yining cultivars were higher than that of wild varieties. [Conclusions]The ferulic medicinal herbs collected from artificial cultivation environment have considerable prospect,and many parts of Ferula herbs can be used for raw materials for preparation of extracts.展开更多
[Objectives] The aim was to determine the optimum process for the extraction of volatile oil from Alpinia zerumbet fruit.[Methods]Steam distillation was used to extract volatile oil from A. zerumbet fruit. Based on th...[Objectives] The aim was to determine the optimum process for the extraction of volatile oil from Alpinia zerumbet fruit.[Methods]Steam distillation was used to extract volatile oil from A. zerumbet fruit. Based on the single factor tests,an orthogonal test was designed to explore the effects of solid-liquid ratio,soaking time,extraction time and grinding degree of material on the extraction rate. The composition of volatile oil from A. zerumbet fruit was analyzed using gas chromatography-mass spectrometry( GC-MS),and the relative mass fraction of each component was determined by peak area normalization. [Results] With volatile oil yield as the index,the optimum extraction process was determined: solid to liquid ratio of 1∶ 10,soaking time of 0. 5 h,grinding degree of passing through 24-mesh sieve and extraction time of 5 h. A total of 29 compounds were isolated. Among them,the contents of α-terpinene( 24. 894%),1,8-terpadiene( 15. 527%) andα-pinene( 6. 982%) were relatively high. [Conclusions]The optimized extraction process for volatile oil from A. zerumbet fruit is stable and reasonable. Under the optimum extraction process,the extraction effect of volatile oil from A. zerumbet fruit was the best. The chemical components of volatile oil from A. zerumbet fruit were determined by GC-MS as α-terpinene( 24. 894%),1,8-terpadiene( 15. 527%) and α-pinene( 6. 982%).展开更多
[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jat...[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jatamansi resource. [Methods]Volatile oil components from different sites of N. jatamansi were identified and isolated by GC-MS. [Results] There were 56 kinds of volatile oil components from different sites of N. jatamansi,in which 39 components from herb of N. jatamansi,39 components from root and rhizome of N. jatamansi,and there were 22 common components. But 1 component in higher concentration had obvious difference. [Conclusions] The herb of N. jatamansi could not completely replace " root and rhizome" of N. jatamansi as the medicine,which was consistent with prior detection result of each physicochemical index from different sites of N. jatamansi. The research could provide the reference for making quality standard of N. jatamansi medicine and rational basis for its further research and development.展开更多
[Objectives]To study the volatile components and antibacterial effects of 11 kinds of Aurantii Fructus Immaturus processed products.[Methods]11 kinds of Aurantii Fructus Immaturus processed products were obtained acco...[Objectives]To study the volatile components and antibacterial effects of 11 kinds of Aurantii Fructus Immaturus processed products.[Methods]11 kinds of Aurantii Fructus Immaturus processed products were obtained according to the traditional processing method,the volatile oil was extracted by steam distillation,and the composition of volatile oil in the 11 kinds of processed products was analyzed by gas chromatography-mass spectrometry(GC-MS).the relative percentage content of each component in these 11 kinds of processed products was determined using the peak area normalization.The drug sensitivity activity of the volatile oil of these 11 kinds of processed products was tested using the K-B paper diffusion method and the minimum inhibition volume fraction of volatile oil of these 11 kinds of processed products was tested using the microdilution method.[Results]The highest yield of volatile oil of 11 kinds of these processed products was baking(5.193%),and the lowest was stir-bake to scorch(1.998%).A total of 36 chemical components were identified from the volatile oils of these 11 kinds of processed products.The components with the most volatile oil were stir-bake to scorch(24 kinds),and the components with the least volatile oil were the method of processing with rice-washed water(15 kinds).They contain 8 kinds of common chemical components,such as limonene,linalool,myrcene,α-pinene.The highest content of limonene came from processing with honey(60.93%),the lowest came from processing with rice-washed water(55.25%);the highest content of linalool came from processing with rice-washed water(7.139%),the lowest came from processing with honey(5.436%);the highest content of myrcene came from processing with honey(1.899%),the lowest came from stir-bake to scorch(1.632%);the highest content ofα-pinene came from raw Aurantii Fructus Immaturus(2.355%),and the lowest came from stir-bake to scorch(1.618%).The volatile oil of these 11 kinds of Aurantii Fructus Immaturus processed products has good antibacterial effect on Escherichia coli and Staphylococcus aureus.[Conclusions]The oil yields of volatile oils of 11 kinds of Aurantii Fructus Immaturus processed products are different,the content of limonene is significantly different,and the changes of other chemical components and their contents are not significantly different.The volatile oil of 11 kinds of Aurantii Fructus Immaturus processed products has certain antibacterial effect.展开更多
The chemical constituents of the essential oil of Euonymus fortune from different areas were compared and analyzed by GC-MS.The results showed that 25 chromatographic peaks were separated and 13 chemical constituents ...The chemical constituents of the essential oil of Euonymus fortune from different areas were compared and analyzed by GC-MS.The results showed that 25 chromatographic peaks were separated and 13 chemical constituents were identified in Binyang County,Nanning City,accounting for 97.25%of the total volatile oil.Twenty nine chromatographic peaks were separated from Rong’an County,Liuzhou City,and 14 chemical constituents were identified,accounting for 89.91%of the total volatile oil.Thirty two chromatographic peaks were separated from Mengshan County,Wuzhou City,and 12 chemical constituents were identified,accounting for 92.16%of the total volatile oil.Twenty eight chromatographic peaks were separated from Rong County,Yulin City,and 11 chemical constituents were identified,accounting for 84.79%of the total volatile oil.Five of the chemical constituents of the volatile oil from the three places of origin are common to the four.They are phytol,palmitic acid,n-nonane,cumene,hexahydrofarnesyl acetone.It can be seen that the main components of volatile oil from different producing areas are different.展开更多
[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manu...[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manufacturers,and further improve the quality control methods.[Methods]The volatile oil in samples was extracted and tested by gas chromatography to collect chromatograms,which were analyze and evaluated by the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine.[Results]Nineteen common peaks were calibrated in the characteristic chromatograms;and the characteristic chromatograms of samples produced by different manufacturers were obviously different.[Conclusions]Controlling the volatile components in Xinyi Biyan Pills by the established characteristic chromatograms of GC is accurate and feasible,and can be used as a quality control method for Xinyi Biyan Pills.展开更多
[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,a...[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.展开更多
Litsea lancilimba Merr.is a Cinnamomum plant of Lauraceae.Phytochemical investigations of the Litsea lancilimba Merr.have resulted in the isolation of Volatile oil,flavonoid glycosides,tannins,alkaloids,lignans,organi...Litsea lancilimba Merr.is a Cinnamomum plant of Lauraceae.Phytochemical investigations of the Litsea lancilimba Merr.have resulted in the isolation of Volatile oil,flavonoid glycosides,tannins,alkaloids,lignans,organic acids and so on.Studies have showed that volatile oil is the main chemical component.Recent trials have demonstrated the efficacy of Litsea lancilimba Merr.in strengthening the function of stomach.The fruits have also been demonstrated to possess anti-inflammatory and bacteriostatic activities.This review deals mainly with the chemical structure and biological activities of Litsea lancilimba Merr.oil.展开更多
Volatile oil(VO)is the main chemical component of common plants in Chrysanthemum genus,and it possesses several beneficial pharmacological properties,including bacteriostatic,antioxidant,anti-tumor,anti-inflammatory,a...Volatile oil(VO)is the main chemical component of common plants in Chrysanthemum genus,and it possesses several beneficial pharmacological properties,including bacteriostatic,antioxidant,anti-tumor,anti-inflammatory,antipyretic,analgesic,antiosteoporotic,antihypertensive,sedative,and hypnotic effects.To date,research on the effective components of Chrysanthemum extract has mainly focused on flavonoids,whereas limited data are available on the chemical constituents and underlying mechanisms of action of the VO components.In this review,the pharmacological activities and mechanisms of VO are comprehensively reviewed with the aim of providing a foundation for further development for medicinal,aromatherapy,and diet therapy applications.展开更多
With a great difference in therapeutic effects of Mahuang(MH, the stems of Ephedra sinica) and Mahuanggen(MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the prese...With a great difference in therapeutic effects of Mahuang(MH, the stems of Ephedra sinica) and Mahuanggen(MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the present study, gas chromatography-mass spectrometry(GC-MS)-based plant metabolomics was employed to compare volatile oil profiles of MH and MHG. The antioxidant activities of volatile oils from MH and MHG were also compared. 32 differential chemical markers were identified according to the variable importance in the projection(VIP) value of orthogonal partial least squares discriminant analysis(OPLS-DA) and P value of Mann-Whitney test. Among them, chemical markers of tetramethylpyrazine(TMP) and α-terpineol were quantified. Their contents were much higher in most MH samples compared with MHG. The antioxidant assay demonstrated that MH had significantly higher free radical-scavenging activity than MHG. Although MH and MHG derived from the same medicinal plant, there was much difference in their volatile oil profiles. MH samples had significantly higher content of two reported pharmacologically important chemical markers of TMP and α-terpineol, which may account for their different antioxidant activities.展开更多
Objective:The objective of this study is to study the best inclusion technology of Lavender-and fennel-mixed volatile oil by beta?cyclodextrin(β-CD) and characterize the final product thereafter.Methods:Using the sat...Objective:The objective of this study is to study the best inclusion technology of Lavender-and fennel-mixed volatile oil by beta?cyclodextrin(β-CD) and characterize the final product thereafter.Methods:Using the saturated water solution method,the volatile β-CD inclusion complex was produced.The effect of volatile oil weight ratio,inclusion temperature and inclusion time on the inclusive quality was studied by measuring the yield of inclusion and inclusion rate of volatile oil as evaluation indexes.The preparation method of inclusion complex was then optimized by the Box-Behnken response surface method.The inclusion complex was characterized by ultraviolet spectrophotometry,thin-layer chromatography,thermogravimetry and differential thermal analysis,and the microscopic imaging method.Results:The optimized conditions were the weight ratio of β-CD to volatile oil was 8.13:1(g/ml).The inclusion temperature was 44°C.The inclusion time was 1 h.Conclusion:We were able to produce an inclusion complex with high inclusion rate of volatile oil and high yield of inclusion using the preparation method mentioned above.Furthermore,the method can also improve the stability of volatile oil in abnormal savda munziq.This study can provide a good reference for the development of new preparations.展开更多
Volatile oil was isolated from the seed of Treculia africana (Moraceae) by solvent extraction and the chemical constituents were identified by means of GC and GC-MS. A total of twelve (12) components were characterize...Volatile oil was isolated from the seed of Treculia africana (Moraceae) by solvent extraction and the chemical constituents were identified by means of GC and GC-MS. A total of twelve (12) components were characterized in the volatile oil. Constituents found inherent were methane;2H-pyran-2-one, 6-[2-E-(3-107491000159-82-745nyl)ethenyl]-4-methoxy-;S-indacen-1(2H)-one, 3,5,6,7-tetrahydro-3,3,4,5,5,8-hexamethyl;7H-Dinaphtho[2,3-b:2.3’-h]carbazole;1,2-Bis[1-(2-hydroxye- thyl)-3,6-diazahomoadamantanty dene-9]hydrazine;3-Nor-3,7-secodichotine,2,7-didehydro-2-deoxy-14-hydroxy-11-methoxy 25-oxo-, (14.alpha.)-;tirucallol;beta-sitosterol;6-Acetyl-3-chloro-2,4-decanobenzoquinoline;Adenosine, N-(3-methyl-3-butenyl)-2’,3’,5’-tris-O-(trime-thylsilyl);Zirconium,dichloro-[dimethylbis-methyl-3H-benz[e]indenyl)silane];Chromone, 7-(3-chloropropoxy)-3-phenyl.展开更多
The eastern main sub-sag(E-MSS)of the Baiyun Sag was the main zone for gas exploration in the deep-water area of the Zhujiang River(Pearl River)Mouth Basin at its early exploration stage,but the main goal of searching...The eastern main sub-sag(E-MSS)of the Baiyun Sag was the main zone for gas exploration in the deep-water area of the Zhujiang River(Pearl River)Mouth Basin at its early exploration stage,but the main goal of searching gas in this area was broken through by the successful exploration of the W3-2 and H34B volatile oil reservoirs,which provides a new insight for exploration of the Paleogene oil reservoirs in the E-MSS.Nevertheless,it is not clear on the distribution of“gas accumulated in the upper layer,oil accumulated in the lower layer”(Gas_(upper)-Oil_(lower))under the high heat flow,different source-rock beds,multi-stages of oil and gas charge,and multi-fluid phases,and not yet a definite understanding of the genetic relationship and formation mechanism among volatile oil,light oil and condensate gas reservoirs,and the migration and sequential charge model of oil and gas.These puzzles directly lead to the lack of a clear direction for oil exploration and drilling zone in this area.In this work,the PVT fluid phase,the origin of crude oil and condensate,the secondary alteration of oil and gas reservoirs,the evolution sequence of oil and gas formation,the phase state of oil and gas migration,and the configuration of fault activity were analyzed,which established the migration and accumulation model of Gas_(upper)-Oil_(lower)cocontrolled by source and heat,and fractionation controlled by facies in the E-MSS.Meanwhile,the fractionation evolution model among common black reservoirs,volatile reservoirs,condensate reservoirs and gas reservoirs is discussed,which proposed that the distribution pattern of Gas_(upper)-Oil_(lower)in the E-MSS is controlled by the generation attribute of oil and gas from source rocks,the difference of thermal evolution,and the fractionation controlled by phases after mixing the oil and gas.Overall,we suggest that residual oil reservoirs should be found in the lower strata of the discovered gas reservoirs in the oil-source fault and diapir-developed areas,while volatile oil reservoirs should be found in the deeper strata near the sag with no oil-source fault area.展开更多
The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistill...The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.展开更多
[Objectives]This study aimed to analyze the volatile oil components of the leaves and bark of Picrasma quassioides.[Methods]The volatile oils in the leaves and bark of P.quassioides were extracted using steam distilla...[Objectives]This study aimed to analyze the volatile oil components of the leaves and bark of Picrasma quassioides.[Methods]The volatile oils in the leaves and bark of P.quassioides were extracted using steam distillation(SD)method and identified with gas chromatography-mass spectrometry(GC-MS).Based on the mass spectrum database(NiST08),combined with artificial spectrum analysis,referring to relevant literature,the base peak and relative abundance were compared to identify the volatile components of the leaves and bark.The relative content of each component was determined by peak area normalization method.[Results]A total of 24 and 23 chemical components were identified in the leaves and bark of P.quassioides,respectively,including 11 common components.[Conclusions]Leaf and bark are different tissues.They contain different volatile chemical components,of which the contents vary greatly.They should be treated differently in the further research and development of P.quassioides resources.展开更多
基金Chinese Medicine Pharmaceutical Key Discipline of Shaanxi province(303061107)National key Research and Development plan(2018-YFC1706904)+2 种基金Discipline Innovation team Project of Shaanxi University of Chinese Medicine(2019-YL11)Shaanxi Province Key subject of pharmacy engineering of Shaanxi Provincial Traditional Chinese Medicine administration(2017001)Key R&D Plan of Shaanxi Province,Development of Nasal Formulations of Ginger Medicinal Components Based on"Component Traditional Chinese Medicine"(2020SF-316).
文摘Background:Exploring the efficacy,potential components,and mechanism of the combination of ginger essential oil and gingerols in the treatment of head wind disease based on network pharmacology technology with content weight.Methods:The experimental groups were divided into:0:10,1:4,1:2,1:1,2:1,4:1,10:0.The relative content(Ri)of the chemical constituents of ginger's volatile oil was determined using gas chromatography-mass spectrometry(GC-MS).Additionally,the physicochemical and biological property parameters(LogP,MDCK,PPB,MW)of the components were considered.To assess the quantitative effect of the components,a grading score was performed,and the quantitative effect index(Ki)was calculated.Subsequently,the target effect index(Ti)was calculated by combining the component-target matching score(Fit score).Using these calculations,the target effect score A was determined under the influence of multiple components targeting different targets.Key targets with A≥1000 were identified.To predict the targets related to head wind disease,the Comparative Toxicogenomics Database(https://ctdbase.org/),Gene Cards(https://www.genecards.org/),and Disgenet database(https://www.disgenet.org/)were utilized.The key targets,obtained from different proportions of ginger's volatile oil and gingerol,were intersected with the predicted targets.This facilitated network pharmacological analysis and verification of the efficacy.Results:The content of volatile oil in ginger demonstrated an impact on key targets associated with the volatile oil group.Each specific combination of volatile oil consistently activated distinct pathways,with variations stemming from changes in content.Experimental testing revealed that different combinations of ginger's volatile oil and gingerol effectively alleviated migraine symptoms in rats.Conclusion:Through the application of content-weighted network pharmacology technology and pharmacodynamic verification,it was determined that altering the ratio between ginger's volatile oil and gingerol leads to variations in potential targets and pathways,consequently impacting its efficacy.
文摘BZ26-6 Oilfield is a kind of deep metamorphic rock buried-hill volatile oilfield in Bohai Sea, China. Its early development plan is restricted due to the simultaneous production of oil and gas in large sections of reservoirs, unclear understanding of formation fluid properties and uncertainty of gas-oil interface. Through theoretical research on phase recovery and experimental analysis of crude oil phase characteristics in the original formation, characteristic parameters of the equilibrium condensate gas fluid are restored and calculated. Through the superimposed phase diagram of volatile oil and condensate gas, BZ26-6 Oilfield is determined to be a volatile oil reservoir with a condensate gas cap, with formation pressure and saturation pressure of 36.1 MPa, respectively. Based on the research results of oil-gas phase behavior characteristics, the thermodynamic equations and equation of state are jointly used to solve the problem, and the content change curves of each component at different depths are drawn. Combined with the sensitivity analysis of numerical simulation, the gas-oil interface is determined to be -3726 m above sea level. The fluid phase analysis software, Fluidmodeler, is used to simulate volatile oil degassing and condensate gas separation experiments. In combination with oil and gas production data obtained through the production test, the specific oil recovery index and the specific gas recovery index are determined to be 0.408 m<sup>3</sup>/(MPa·d·m) and 1195 m<sup>3</sup>/(MPa·d·m), respectively. And the reasonable production capacity prediction is conducted on the early development of BZ26-6 Oilfield. The research results can provide a theoretical basis for the efficient development of similar complex oil and gas reservoirs.
基金the National Natural Science Foundation of China(No.31200264)the Fundamental Research Funds for Central Universities(South-Central University for NationalitiesNo.CZY19028,No.CZY20048).
文摘This study was designed to investigate the anti-inflammatory effects of volatile oil of Platycladus orientalis(L.)Franco leaves(VOPF)and the underlying molecular mechanisms by using the non-infectious inflammation rat models and infectious inflammation mouse models.Ear swelling and intraperitoneal capillary permeability in mice,and carrageenan-induced toe swelling and cotton ball-induced granuloma in rats were used to reveal anti-inflammatory effects of VOPF.Moreover,the lipopolysaccharide(LPS)-induced mouse model of acute lung injury was used to explore the anti-inflammatory mechanism of VOPF.The results showed that VOPF could significantly inhibit auricular swelling,intraperitoneal capillary permeability in mice,and reduce granuloma swelling and paw swelling in rats.Furthermore,it significantly alleviated the pathological damage of the lung tissue.In addition,VOPF could reduce the contents of IL-1β and TNF-αand increase the content of IL-10 in the serum.It had little effect on the expression of p65 but reduced the phosphorylation level of p65 and IκB in NF-κB pathway.In conclusion,VOPF has anti-inflammatory effects and the mechanisms involve the down-regulation of the phosphorylation levels of p65 and IκB and blockage of the NF-κB signaling pathway.
文摘[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-MS method combined with retention index and peak area normalization method was used for qualitative and quantitative analysis.[Results]The volatile oils of mango leaves in southern provinces contained high content of terpenes,such asα-gurjunene(18.39%-34.13%),(+)-viridiflorene(8.95%-16.30%)and(E)-β-ocimene(3.49%-7.63%).The volatile oils components of mango leaves were significantly different in different provinces,for example,mango leaves produced in Hainan Province contained a large amount ofβ-selinene(23.00%),but not in other provinces.[Conclusions]The origin had a great influence on the composition and contents of volatile oils in mango leaves.
文摘Rosa x alba is cultivated almost exclusively for the production of aromatic water and fruits widely used as ingredients in some traditional food preparations sold commercially.Results of the proximate and elemental analyses revealed that flowers exhibited higher moisture content while the dry matter content was higher in leaves.Total fat and ash was higher in fruits when compared with leaves and flowers.All studied organs were found to be rich in essential mineral such as K,Ca,P and Mg.Qualitative and quantitative analysis of the volatile oils revealed different composition patterns between organs.Linalool and geraniol were found as major constituents of the leaf oil whereas 2-phenethyl alcohol and eugenol were the major constituents of the floral oil.In contrast,fruits showed very distinct composition and alkanes/alkenes were found to have the major contribution.The present composition could justify the traditional use of Rosa x alba which could be considered as a potential source of essential minerals and volatile constituents.
基金Supported by Traditional Chinese Medicine Project of Ministry of Industry and Information Technology"Wild Tending and Artificial Cultivation Base Construction of Endangered Medicinal Material Ferula"(2015)Yili Botanical Garden Planning and Design and Pre-construction Project(20130122-1)
文摘[Objectives] To increase the reserves distribution and planting area of Ferula resources,protect wild Ferula resources,make comparative analysis on wild and cultivated varieties of Ferula medicinal materials,and provide references for quality evaluation of Ferula herbs collected from cultivation environment. [Methods] Volatile oil determination and high performance liquid chromatography were used to measure volatile oil and ferulic acid in different parts of wild and cultivated varieties of Ferula. [Results] In the volatile oil measured from artificially pressed Ferula lipid,wild varieties in different places conformed to the pharmacopoeia standard( volatile oil content ≥10%); in cultivated varieties,F. fukanensis K. M. Shen and F. sinkiangensis K. M. shen reached the pharmacopoeia standard. According to measurement results of ferulic acid,the ferulic acid content of roots and leaves of Altay,Fukang,and Yining cultivars were higher than that of wild varieties. [Conclusions]The ferulic medicinal herbs collected from artificial cultivation environment have considerable prospect,and many parts of Ferula herbs can be used for raw materials for preparation of extracts.
基金Supported by Guizhou Provincial Project for Modernization and Industrialization of Traditional Chinese Medicine(QKHZY[2012]5001-5)
文摘[Objectives] The aim was to determine the optimum process for the extraction of volatile oil from Alpinia zerumbet fruit.[Methods]Steam distillation was used to extract volatile oil from A. zerumbet fruit. Based on the single factor tests,an orthogonal test was designed to explore the effects of solid-liquid ratio,soaking time,extraction time and grinding degree of material on the extraction rate. The composition of volatile oil from A. zerumbet fruit was analyzed using gas chromatography-mass spectrometry( GC-MS),and the relative mass fraction of each component was determined by peak area normalization. [Results] With volatile oil yield as the index,the optimum extraction process was determined: solid to liquid ratio of 1∶ 10,soaking time of 0. 5 h,grinding degree of passing through 24-mesh sieve and extraction time of 5 h. A total of 29 compounds were isolated. Among them,the contents of α-terpinene( 24. 894%),1,8-terpadiene( 15. 527%) andα-pinene( 6. 982%) were relatively high. [Conclusions]The optimized extraction process for volatile oil from A. zerumbet fruit is stable and reasonable. Under the optimum extraction process,the extraction effect of volatile oil from A. zerumbet fruit was the best. The chemical components of volatile oil from A. zerumbet fruit were determined by GC-MS as α-terpinene( 24. 894%),1,8-terpadiene( 15. 527%) and α-pinene( 6. 982%).
基金Supported by the Applied Basic Research Project in Sichuan(2018)National Science and Technology Support Program(2015BAC05B02)Innovative Research Projects of Graduate Students in Southwest Minzu University in 2017(CX2017SZ085)
文摘[Objectives] Volatile oil components of traditional drug use site " root and rhizome" of N. jatamansi and herb were identified and contrasted,which aimed to provide the basis for rationally using wild N. jatamansi resource. [Methods]Volatile oil components from different sites of N. jatamansi were identified and isolated by GC-MS. [Results] There were 56 kinds of volatile oil components from different sites of N. jatamansi,in which 39 components from herb of N. jatamansi,39 components from root and rhizome of N. jatamansi,and there were 22 common components. But 1 component in higher concentration had obvious difference. [Conclusions] The herb of N. jatamansi could not completely replace " root and rhizome" of N. jatamansi as the medicine,which was consistent with prior detection result of each physicochemical index from different sites of N. jatamansi. The research could provide the reference for making quality standard of N. jatamansi medicine and rational basis for its further research and development.
基金the First Phase of Teaching Ability Training Program for Young Backbone Teachers of Guangxi University of Chinese Medicine(04B1805815)Program of Key Laboratory for Extraction and Purification and Quality Analysis of TCM in 2014(Gui Jiao Ke Yan[2014]No.6)。
文摘[Objectives]To study the volatile components and antibacterial effects of 11 kinds of Aurantii Fructus Immaturus processed products.[Methods]11 kinds of Aurantii Fructus Immaturus processed products were obtained according to the traditional processing method,the volatile oil was extracted by steam distillation,and the composition of volatile oil in the 11 kinds of processed products was analyzed by gas chromatography-mass spectrometry(GC-MS).the relative percentage content of each component in these 11 kinds of processed products was determined using the peak area normalization.The drug sensitivity activity of the volatile oil of these 11 kinds of processed products was tested using the K-B paper diffusion method and the minimum inhibition volume fraction of volatile oil of these 11 kinds of processed products was tested using the microdilution method.[Results]The highest yield of volatile oil of 11 kinds of these processed products was baking(5.193%),and the lowest was stir-bake to scorch(1.998%).A total of 36 chemical components were identified from the volatile oils of these 11 kinds of processed products.The components with the most volatile oil were stir-bake to scorch(24 kinds),and the components with the least volatile oil were the method of processing with rice-washed water(15 kinds).They contain 8 kinds of common chemical components,such as limonene,linalool,myrcene,α-pinene.The highest content of limonene came from processing with honey(60.93%),the lowest came from processing with rice-washed water(55.25%);the highest content of linalool came from processing with rice-washed water(7.139%),the lowest came from processing with honey(5.436%);the highest content of myrcene came from processing with honey(1.899%),the lowest came from stir-bake to scorch(1.632%);the highest content ofα-pinene came from raw Aurantii Fructus Immaturus(2.355%),and the lowest came from stir-bake to scorch(1.618%).The volatile oil of these 11 kinds of Aurantii Fructus Immaturus processed products has good antibacterial effect on Escherichia coli and Staphylococcus aureus.[Conclusions]The oil yields of volatile oils of 11 kinds of Aurantii Fructus Immaturus processed products are different,the content of limonene is significantly different,and the changes of other chemical components and their contents are not significantly different.The volatile oil of 11 kinds of Aurantii Fructus Immaturus processed products has certain antibacterial effect.
基金the Yao Medicine Quality Standard Project of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicinethe Basic Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Universities(P18037)General Project of Guangxi University of Chinese Medicine(2017MS024)。
文摘The chemical constituents of the essential oil of Euonymus fortune from different areas were compared and analyzed by GC-MS.The results showed that 25 chromatographic peaks were separated and 13 chemical constituents were identified in Binyang County,Nanning City,accounting for 97.25%of the total volatile oil.Twenty nine chromatographic peaks were separated from Rong’an County,Liuzhou City,and 14 chemical constituents were identified,accounting for 89.91%of the total volatile oil.Thirty two chromatographic peaks were separated from Mengshan County,Wuzhou City,and 12 chemical constituents were identified,accounting for 92.16%of the total volatile oil.Twenty eight chromatographic peaks were separated from Rong County,Yulin City,and 11 chemical constituents were identified,accounting for 84.79%of the total volatile oil.Five of the chemical constituents of the volatile oil from the three places of origin are common to the four.They are phytol,palmitic acid,n-nonane,cumene,hexahydrofarnesyl acetone.It can be seen that the main components of volatile oil from different producing areas are different.
基金Guangxi Key R&D Program Project (GK AB19110027).
文摘[Objectives]This study was conducted to establish characteristic chromatograms of of the volatile oil of Xinyi Biyan Pills by gas chromatography,discover possible problems in the production processes of different manufacturers,and further improve the quality control methods.[Methods]The volatile oil in samples was extracted and tested by gas chromatography to collect chromatograms,which were analyze and evaluated by the similarity evaluation software of chromatographic fingerprints of traditional Chinese medicine.[Results]Nineteen common peaks were calibrated in the characteristic chromatograms;and the characteristic chromatograms of samples produced by different manufacturers were obviously different.[Conclusions]Controlling the volatile components in Xinyi Biyan Pills by the established characteristic chromatograms of GC is accurate and feasible,and can be used as a quality control method for Xinyi Biyan Pills.
基金Supported by Undergraduate Innovation and Entrepreneurship Project of Department of Education of Guangxi Zhuang Autonomous Region(No:201813643063).
文摘[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.
文摘Litsea lancilimba Merr.is a Cinnamomum plant of Lauraceae.Phytochemical investigations of the Litsea lancilimba Merr.have resulted in the isolation of Volatile oil,flavonoid glycosides,tannins,alkaloids,lignans,organic acids and so on.Studies have showed that volatile oil is the main chemical component.Recent trials have demonstrated the efficacy of Litsea lancilimba Merr.in strengthening the function of stomach.The fruits have also been demonstrated to possess anti-inflammatory and bacteriostatic activities.This review deals mainly with the chemical structure and biological activities of Litsea lancilimba Merr.oil.
基金funded by the National Natural Science Foundation of China(82260695)the Jiangxi Provincial Natural Science Foundation(20232ACB206062,20212ACB206004)+2 种基金Young Jinggang Scholar of Jiangxi Province and New Century Talents Project of Jiangxi Province(2017082,2020028)the Science and Technology Innovation Team of Jiangxi University of Chinese Medicine(CXTD22001,CXTD22006)Project of College Students’Innovation and Entrepreneurship Training Program of Jiangxi University of Chinese Medicine.
文摘Volatile oil(VO)is the main chemical component of common plants in Chrysanthemum genus,and it possesses several beneficial pharmacological properties,including bacteriostatic,antioxidant,anti-tumor,anti-inflammatory,antipyretic,analgesic,antiosteoporotic,antihypertensive,sedative,and hypnotic effects.To date,research on the effective components of Chrysanthemum extract has mainly focused on flavonoids,whereas limited data are available on the chemical constituents and underlying mechanisms of action of the VO components.In this review,the pharmacological activities and mechanisms of VO are comprehensively reviewed with the aim of providing a foundation for further development for medicinal,aromatherapy,and diet therapy applications.
基金financially supported by the National Science Foundation of China(No.81302733)the research project of Chinese Ministry of education(No.113036A)+3 种基金the Program for Jiangsu province Innovative Research Team,the Program for New Century Excellent Talents in University(No.NCET-13-1036)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Fundamental Research Funds for the Central Universities(No.JKZD2013004)the Open Project Program of State Key Laboratory of Natural Medicines,China Pharmaceutical University(Nos.SKLNMZZYQ 201303 and SKLNMKF201220)
文摘With a great difference in therapeutic effects of Mahuang(MH, the stems of Ephedra sinica) and Mahuanggen(MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the present study, gas chromatography-mass spectrometry(GC-MS)-based plant metabolomics was employed to compare volatile oil profiles of MH and MHG. The antioxidant activities of volatile oils from MH and MHG were also compared. 32 differential chemical markers were identified according to the variable importance in the projection(VIP) value of orthogonal partial least squares discriminant analysis(OPLS-DA) and P value of Mann-Whitney test. Among them, chemical markers of tetramethylpyrazine(TMP) and α-terpineol were quantified. Their contents were much higher in most MH samples compared with MHG. The antioxidant assay demonstrated that MH had significantly higher free radical-scavenging activity than MHG. Although MH and MHG derived from the same medicinal plant, there was much difference in their volatile oil profiles. MH samples had significantly higher content of two reported pharmacologically important chemical markers of TMP and α-terpineol, which may account for their different antioxidant activities.
基金supported by the National Natural Science Foundation of China(no:81660667)
文摘Objective:The objective of this study is to study the best inclusion technology of Lavender-and fennel-mixed volatile oil by beta?cyclodextrin(β-CD) and characterize the final product thereafter.Methods:Using the saturated water solution method,the volatile β-CD inclusion complex was produced.The effect of volatile oil weight ratio,inclusion temperature and inclusion time on the inclusive quality was studied by measuring the yield of inclusion and inclusion rate of volatile oil as evaluation indexes.The preparation method of inclusion complex was then optimized by the Box-Behnken response surface method.The inclusion complex was characterized by ultraviolet spectrophotometry,thin-layer chromatography,thermogravimetry and differential thermal analysis,and the microscopic imaging method.Results:The optimized conditions were the weight ratio of β-CD to volatile oil was 8.13:1(g/ml).The inclusion temperature was 44°C.The inclusion time was 1 h.Conclusion:We were able to produce an inclusion complex with high inclusion rate of volatile oil and high yield of inclusion using the preparation method mentioned above.Furthermore,the method can also improve the stability of volatile oil in abnormal savda munziq.This study can provide a good reference for the development of new preparations.
文摘Volatile oil was isolated from the seed of Treculia africana (Moraceae) by solvent extraction and the chemical constituents were identified by means of GC and GC-MS. A total of twelve (12) components were characterized in the volatile oil. Constituents found inherent were methane;2H-pyran-2-one, 6-[2-E-(3-107491000159-82-745nyl)ethenyl]-4-methoxy-;S-indacen-1(2H)-one, 3,5,6,7-tetrahydro-3,3,4,5,5,8-hexamethyl;7H-Dinaphtho[2,3-b:2.3’-h]carbazole;1,2-Bis[1-(2-hydroxye- thyl)-3,6-diazahomoadamantanty dene-9]hydrazine;3-Nor-3,7-secodichotine,2,7-didehydro-2-deoxy-14-hydroxy-11-methoxy 25-oxo-, (14.alpha.)-;tirucallol;beta-sitosterol;6-Acetyl-3-chloro-2,4-decanobenzoquinoline;Adenosine, N-(3-methyl-3-butenyl)-2’,3’,5’-tris-O-(trime-thylsilyl);Zirconium,dichloro-[dimethylbis-methyl-3H-benz[e]indenyl)silane];Chromone, 7-(3-chloropropoxy)-3-phenyl.
基金The Major Science and Technology Project of China National Offshore Oil Corporation during the“14th Five-Year Plan”under contact No.KJGG2022-0103-03。
文摘The eastern main sub-sag(E-MSS)of the Baiyun Sag was the main zone for gas exploration in the deep-water area of the Zhujiang River(Pearl River)Mouth Basin at its early exploration stage,but the main goal of searching gas in this area was broken through by the successful exploration of the W3-2 and H34B volatile oil reservoirs,which provides a new insight for exploration of the Paleogene oil reservoirs in the E-MSS.Nevertheless,it is not clear on the distribution of“gas accumulated in the upper layer,oil accumulated in the lower layer”(Gas_(upper)-Oil_(lower))under the high heat flow,different source-rock beds,multi-stages of oil and gas charge,and multi-fluid phases,and not yet a definite understanding of the genetic relationship and formation mechanism among volatile oil,light oil and condensate gas reservoirs,and the migration and sequential charge model of oil and gas.These puzzles directly lead to the lack of a clear direction for oil exploration and drilling zone in this area.In this work,the PVT fluid phase,the origin of crude oil and condensate,the secondary alteration of oil and gas reservoirs,the evolution sequence of oil and gas formation,the phase state of oil and gas migration,and the configuration of fault activity were analyzed,which established the migration and accumulation model of Gas_(upper)-Oil_(lower)cocontrolled by source and heat,and fractionation controlled by facies in the E-MSS.Meanwhile,the fractionation evolution model among common black reservoirs,volatile reservoirs,condensate reservoirs and gas reservoirs is discussed,which proposed that the distribution pattern of Gas_(upper)-Oil_(lower)in the E-MSS is controlled by the generation attribute of oil and gas from source rocks,the difference of thermal evolution,and the fractionation controlled by phases after mixing the oil and gas.Overall,we suggest that residual oil reservoirs should be found in the lower strata of the discovered gas reservoirs in the oil-source fault and diapir-developed areas,while volatile oil reservoirs should be found in the deeper strata near the sag with no oil-source fault area.
基金The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)the Coordenacao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES)and the Fundacao de Amparoà Pesquisa do Estado de Minas Gerais(FAPEMIG)for financial support,a scholarship and a PVNS fellowship(D.L.Nelson).
文摘The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.
基金Supported by Open Project of Development and Research Center for China-ASEAN Traditional Medicine(0501801708)Guangxi First-class Discipline:Chinese Materia Medica(Scientific Research of Guangxi Education Department[2018]No.12)Guangxi Collaborative Innovation Center for Scientific Achievements Transformation and Application on Traditional Chinese Medicine(05020058).
文摘[Objectives]This study aimed to analyze the volatile oil components of the leaves and bark of Picrasma quassioides.[Methods]The volatile oils in the leaves and bark of P.quassioides were extracted using steam distillation(SD)method and identified with gas chromatography-mass spectrometry(GC-MS).Based on the mass spectrum database(NiST08),combined with artificial spectrum analysis,referring to relevant literature,the base peak and relative abundance were compared to identify the volatile components of the leaves and bark.The relative content of each component was determined by peak area normalization method.[Results]A total of 24 and 23 chemical components were identified in the leaves and bark of P.quassioides,respectively,including 11 common components.[Conclusions]Leaf and bark are different tissues.They contain different volatile chemical components,of which the contents vary greatly.They should be treated differently in the further research and development of P.quassioides resources.
