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Cloning of a putative extracellular Cu/Zn superoxide dismutase and functional differences of superoxide dismutases in invasive and indigenous whiteflies 被引量:2
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作者 Xian-Long Gao Jun-Min Li +4 位作者 Hong-Xing Xu Gen-Hong Yan Min Jiu Shu-Sheng Liu Xiao-Wei Wang 《Insect Science》 SCIE CAS CSCD 2015年第1期52-64,共13页
Superoxide dismutases (SODs) are a group of important antioxidant defense enzymes. In this study, a putative extracellular Cu/Zn superoxide dismutase (ecCuZnSOD) complementary DNA was cloned and characterized from... Superoxide dismutases (SODs) are a group of important antioxidant defense enzymes. In this study, a putative extracellular Cu/Zn superoxide dismutase (ecCuZnSOD) complementary DNA was cloned and characterized from the whitefly, Bemisia tabaci. Quantitative polymerase chain reaction analysis showed that the expression level of BtecCuZnSOD was more than 10-fold higher in the invasive Middle East Asia Minor 1 (MEAM1) than in the native Asia II 3 species of the B. tabaci species complex. After exposure to low temperature (4 ℃), the expression of Bt-ecCuZnSOD gene was significantly up-regulated in MEAM1 but not in Asia II 3. Furthermore, the expression level ofB. tabaci intracellular CuZnSOD (Bt-icCuZnSOD), Bt-ecCuZnSOD and mitochondrial MnSOD (Bt-mMnSOD) was compared after transferring MEAM1 and Asia II 3 whiteflies from favorable (cotton) to unfavorable host plants (tobacco). On cotton, both CuZnSOD genes were expressed at a higher level in MEAM1 compared with Asia II 3. Interestingly, after transferring onto tobacco, the expression of Bt-ecCuZnSOD was significantly induced in Asia II 3 but not in MEAM1. On the other hand, while Bt-mMnSOD was expressed equally in both species on cotton, Bt-mMnSOD messenger RNA was up-regulated in MEAM 1 on tobacco. Consistently, enzymatic activity assays of CuZnSOD and MnSOD demonstrated that CuZnSOD might play an important protective role against oxidative stress in Asia II 3, whereas MnSOD activation was critical for MEAM1 whiteflies during host adaptation. Taken together, our results suggest that the successful invasion ofMEAM 1 is correlated with its constitutive high activity of CuZnSOD and inducible expression of MnSOD under stress conditions. 展开更多
关键词 Bemisia tabaci extracellular Cu/Zn superoxide dismutase oxidative strees superoxide dismutase WHITEFLY
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Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts
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作者 Dan-Tong Zhu Qiong Rao +3 位作者 Chi Zou Fei-Xue Ban Juan-Juan Zhao Shu-Sheng Liu 《Insect Science》 SCIE CAS CSCD 2022年第2期539-549,共11页
Nutritional mutualism between insects and symbiotic bacteria is widespread.The various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple sec... Nutritional mutualism between insects and symbiotic bacteria is widespread.The various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary symbionts.It is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect hosts.Although effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well understood.We examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the B.tabaci whitefly complex,provisionally named as Asia II 3 and China 1.Genomic sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B vitamins.We then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously reported.The analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were identical.Our findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host whiteflies.Although phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B vitamins.Such metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution. 展开更多
关键词 SYMBIONT B vitamins COMPLEMENT evolution WHITEFLY
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Patterns of host cell inheritance in the bacterial symbiosis of whiteflies
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作者 Xiao-Rui Xu Na-Na Li +2 位作者 Xi-Yu Bao Angela E.Douglas Jun-Bo Luan 《Insect Science》 SCIE CAS CSCD 2020年第5期938-946,共9页
Whiteflies possess bacterial symbionts Candidatus Portiera aleyrodidium that are housed in specialized cells called bacteriocytes and are faithfully transmitted via the ovary to insect offspring.In one whitefly specie... Whiteflies possess bacterial symbionts Candidatus Portiera aleyrodidium that are housed in specialized cells called bacteriocytes and are faithfully transmitted via the ovary to insect offspring.In one whitefly species studied previously,Bemisia tabaci MEAMI,transmission is mediated by somatic inheritance of bacteriocytes,with a single bacteriocyte transferred to each oocyte and persisting through embryogenesis to the next generation.Here,we investigate the mode of bacteriocyte transmission in two whiefly species,B.tabaci MED,the sister species of MEAM1,and the phylogenetically distant species Trialeurodes vaporariorum.Microsatllite analysis supported by microscopical studies demonstrates that B.tabaci MED bacteriocytes are genetically different from other somatic cells and persist through embryogenesis,as for MEAMI,but T.vaporariorum bacteriocytes are genetically identical to other somatic cells of the insect,likely mediated by the degradation of maternal bacteriocytes in the embryo.These two alternative modes of transmission provide a first demonstration among insect symbioses that the cellular processes underlying vertical transmission of bacterial symbionts can diversify among related host species associated with a single lineage of symbiotic bacteria. 展开更多
关键词 bacteriocyte INHERITANCE somatic cells symbiont transmission WHITEFLY
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Contribution to the knowledge of Pentaleyrodes Takahashi(Hemiptera:Aleyrodidae) from China
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作者 王吉锐 赖秋利 +1 位作者 徐志宏 杜予州 《Entomotaxonomia》 CSCD 2017年第1期43-51,共9页
Four species in the genus Pentaleyrodes Takahashi(Hemiptera:Aleyrodidae) from China,P.yasumatsui Takahashi,P.hongkongensis Takahashi,P.cinnamomi(Takahashi) and P.linderae Chou & Yan are reported in this paper.P.ya... Four species in the genus Pentaleyrodes Takahashi(Hemiptera:Aleyrodidae) from China,P.yasumatsui Takahashi,P.hongkongensis Takahashi,P.cinnamomi(Takahashi) and P.linderae Chou & Yan are reported in this paper.P.yasumatsui,a newly recorded species to China,and P.hongkongensis,a newly recorded species to the Chinese Mainland are redescribed,and the identification of P.cinnamomi and P.linderae is discussed.Morphological illustrations and photographs from a scanning electron microscope(SEM)of these four species are provided.An identification key to these Pentaleyrodes species is given. 展开更多
关键词 whiteflies TAXONOMY new record KEY
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Effect of Four Pesticides on Controlling Autumn Cowpea Greenhouse Whitefly
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作者 彭昌家 白体坤 +2 位作者 丁攀 冯礼斌 杨宇衡 《Agricultural Science & Technology》 CAS 2016年第9期2119-2123,共5页
In order to mitigate the occurrence and damage of cowpea whitefly, reduce chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design andstatistical analysi... In order to mitigate the occurrence and damage of cowpea whitefly, reduce chemical pesticide consumption, residue and environmental pollution, the methods of forecasting, randomized block design andstatistical analysis were used in field efficacy trials, to study the control effect of cowpea anthracnose by usingchemical pesticides dinotefuran, acetamiprid, pymetrozine, and bio-pesticide avermectin. The results showed that after applying 1.85% avermectin EC 375 g, 20% dinotefuran SP 600 g, 5% acetamiprid WP 600 g, and 25% pymetrozine WP 600 g (control pesticide) per hm2 once every 5-8 days, and 3 times continuously underserious autumn occurrence of greenhouse whitefly, the control efficacy was 90.9%, 97.0%, 88.0%, 93.9%respectively on the 7th day after the pesticides application; 97.4%, 92.1%, 84.2%, 89.4% respectively on the 14th day after the pesticides application; and 95.6%, 86.7%, 82.2%, 84.5%, respectively on the 20th day afterthe pesticides application. The control effects among avermectin, acetamiprid and pymetrozine were not significantly different, while the effects of avermectin and pymetrozine were significantly higher than that of acetamiprid on the 7th day. The control effects among avermectin, dinotefuran and pymetrozine were not significantly different, while significantly higher than that of acetamiprid on the 14th day. The control effect of avermectin was significantly higher than that of dinotefuran, acetamiprid and pymetrozine on the 20th day. Meanwhile, the control effect of avermectin was slightly lower, higher and much higher than that of dinotefuran on the 7th, 14th, 20th day respectively after the treatment. These results indicated that the four pesticides,especiaLly biopesticide avermectin, were ideal pesticides, which could not only be used for controlling cowpea whitefly, but also realize modern, green, organic and ecological agricultural production. 展开更多
关键词 COWPEA Greenhouse whitefly Bio-pesticide AVERMECTINS Chemical pesticides DINOTEFURAN ACETAMIPRID PYMETROZINE Control effect
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Extracts of Melia azedarach Increases Mulberry Whitefly Mortality without Affecting Silkworm Survivorship 被引量:1
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作者 Antonios E. Tsagkarakis Rodanthi Babili +1 位作者 Paschalis Harizanis Dionyssios Ch. Perdikis 《Advances in Entomology》 2016年第5期293-298,共6页
Melia azedarach L. extracts were studied in comparison with selected synthetic in-secticides against the mulberry whiteflies Pealiusma chili and P. mori under labora-tory conditions. Morus alba (cv. “Early Spring”) ... Melia azedarach L. extracts were studied in comparison with selected synthetic in-secticides against the mulberry whiteflies Pealiusma chili and P. mori under labora-tory conditions. Morus alba (cv. “Early Spring”) leaves infested by mulberry white-flies were exposed to Melia extracts of green fruits, l-cyhalothrin (10% a.i.) and con-trol. Also, white mulberry leaves after treatment with the same insecticides were provided to silkworms for consumption. Results have shown that there were signif-icant differences in the nymphal mortality of the whiteflies among treatments. Melia extracts and l-cyhalothrin were effective in increasing whitefly mortality 24 h after application. Furthermore, Melia extracts didn’t affect silkworm survivorship, which was severely affected by l-cyhalothrin treatment, since all the silkworms fed on treated leaves were found dead. Thus, these extracts might be considered a potential alternative in management of the mulberry whiteflies. 展开更多
关键词 Mulberry whiteflies Control Melia azedarach Extract SILKWORM
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Contribution to the knowledge of Chinese species of Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae)
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作者 Jirui WANG Yuzhou DU Zhihong XU 《Entomotaxonomia》 CSCD 2019年第4期266-272,共7页
Two species in the genus Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae) from China, A. callicarpae Takahashi and A. styraci Takahashi are reported in this paper. The diagnosis, distribution, host plant and morpholo... Two species in the genus Acanthaleyrodes Takahashi(Hemiptera: Aleyrodidae) from China, A. callicarpae Takahashi and A. styraci Takahashi are reported in this paper. The diagnosis, distribution, host plant and morphological illustrations of these two species are provided. An identification key to the puparia of Chinese Acanthaleyrodes species is given. 展开更多
关键词 whiteflies TAXONOMY new record key China
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Species Concepts as Applied to the Whitefly Bemisia tabaci Systematics:How Many Species Are There? 被引量:78
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作者 LIU Shu-sheng John Colvin Paul J De Barro 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期176-186,共11页
The worldwide distribution and extensive genetic diversity of the whitefly,Bemisia tabaci,has long been recognized.However,the levels of separation within B.tabaci and the nomenclature of the various genetic groups ha... The worldwide distribution and extensive genetic diversity of the whitefly,Bemisia tabaci,has long been recognized.However,the levels of separation within B.tabaci and the nomenclature of the various genetic groups have been a subject of debate.Recent phylogenetic analyses indicate that B.tabaci is a complex composed of 28 morphologically indistinguishable species.In this article,we first review the debate and difficulties associated with B.tabaci's taxonomy and systematics,and argue for the need to apply the biological species concept in order to elucidate B.tabaci's systematics.We summarize the accumulated genetic and behavioural data on reproductive incompatibilities evident amongst phylogenetic mtCOI groups of B.tabaci.Crossing studies have been conducted with 14 of the 28 putative species covering 54 reciprocal inter-species pairs,and observations on mating behaviour have been conducted for seven species pairs.Data from both crossing trials and behavioural observations indicate a consistent pattern of reproductive isolation among the putative species.We then discuss the technical and conceptual complexities associated with crossing experiments and behavioural observations designed to reveal reproductive incompatibility.Finally,we elaborate on a strategy for further clarifying the pattern of reproductive isolation between B.tabaci groups and propose future research directions on the systematics of this complex. 展开更多
关键词 whitefly systematics biological species cryptic species phylogenetic species reproductive isolation mating behavior TAXONOMY
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Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis 被引量:15
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作者 GUO Xiao-jun RAO Qiong +3 位作者 ZHANG Fan LUO Chen ZHANG Hong-yu GAO Xi-wu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期206-214,共9页
The whitefly Bemisia tabaci are considered as a taxonomically complex that contained some destructive pests.Two of the most prevalent cryptic species are B.tabaci Middle East-Asia Minor 1(MEAM1)and Mediterranean(ME... The whitefly Bemisia tabaci are considered as a taxonomically complex that contained some destructive pests.Two of the most prevalent cryptic species are B.tabaci Middle East-Asia Minor 1(MEAM1)and Mediterranean(MED).In an extensive field survey of the B.tabaci complex present throughout part of China from 2004 to 2007,we obtained 93 samples of B.tabaci from 22 provinces.We determined that these Chinese haplotypes included 2 invasive species(MEAM1 and MED),and 4 indigenous cryptic species(Asia II 1,Asia II 3,China 3 and Asia II 7)by sequencing mitochondrial cytochrome oxidose one gene(mtCOI).The diversity and genetic differentiation of a subset of 19 populations of B.tabaci were studied using cDNA amplified fragment length polymorphism(AFLP).Prior to 2007,MEAM1 was a dominant species in many provinces in China.By 2007,MED was dominant in 11 provinces.Both invasive and indigenous species were simultaneously found in some regions.Indigenous species of B.tabaci were found in six provinces in southern China.MED and MEAM1 have broad ranges of host plants,and indigenous species appeared to have much narrower host ranges.All Asia II 3 samples were found on cotton except one on aubergine.China 3 has more host plants than Asia II 3.Twelve samples of China 3 were collected from sweet potato,Japanese hop,squash and cotton.A total of 677 reproducible bands amplified with 5 AFLP primer combinations were obtained.The highest proportion of polymorphic bands was 98.7% and the lowest was 91.9%.Unweighted pair-group method analysis indicated that the clustering was independent of the different species.MED showed the lowest degree of similarity than the other species.The data indicate that both MEAM1and MED were rapidly established in China. 展开更多
关键词 Bemisia tabaci MTCOI CDNA-AFLP DIVERSITY WHITEFLY
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Density and Seasonal Dynamics of Bemisia tabaci(Gennadius) Mediterranean on Common Crops and Weeds Around Cotton Fields in Northern China 被引量:10
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作者 ZHANG Xiao-ming YANG Nian-wan +1 位作者 WAN Fang-hao Gabor L Lvei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第10期2211-2220,共10页
The density seasonal dynamics of Bemisia tabaci MED were evaluated over two years in a cotton-growing area in Langfang, Hebei Province, northern China on cotton (Gossypium hirsutum L.) and six other co-occurring com... The density seasonal dynamics of Bemisia tabaci MED were evaluated over two years in a cotton-growing area in Langfang, Hebei Province, northern China on cotton (Gossypium hirsutum L.) and six other co-occurring common plants, common ragweed (Ambrosia artemisiifolia L.), piemarker (Abutilon theophrasti Medicus), sunflower (Helianthus annuus L.), sweet potato (Ipomoea batatas L.), soybean (Glycine max L.), and maize (Zea mays L.). The whitefly species identity was repeatedly tested and confirmed; seasonal dynamics on the various host plants were standardized by the quartile method. B. tabaci MED appeared on weeds (the common ragweed and piemarker) about 10 days earlier than on cotton, or the other cultivated plants. The peak population densities were observed over a span of 2 to 3 weeks on cotton, starting in early (2010) or mid-August (2011). The common ragweed growing adjacent to cotton supported the highest B. tabaci densities (no. on 100 cm2 leaf surface), 12-22 fold higher than on cotton itself. Sunflower supported more B. tabaci than the other plants, and about 1.5-2 fold higher than cotton did, Our results indicate that weeds (esp. the common ragweed) around cotton fields could increase the population density of B. tabaci MED on cotton, while sunflower could act as a trap crop for decreasing pest pressure on cotton. 展开更多
关键词 Bemisia tabaci WHITEFLY cotton SUNFLOWER RAGWEED population dynamics seasonal dynamics quartile method
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Bemisia tabaci Phylogenetic Groups in India and the Relative Transmission Efficacy of Tomato leaf curl Bangalore virus by an Indigenous and an Exotic Population 被引量:8
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作者 R V Chowda-Reddy M Kirankumar +4 位作者 Susan E Seal V Muniyappa Girish B Valand M R Govindappa John Colvin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期235-248,共14页
Bemisia tabaci adults from various host-plant species were collected from 31 regions across India.266 B.tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with di... Bemisia tabaci adults from various host-plant species were collected from 31 regions across India.266 B.tabaci samples were first screened by RAPD-PCR to examine molecular variability and to select individuals with different fingerprints.Host-plant and region of collection were then used to select 25 individuals for PCR amplification and sequencing of their partial mitochondrial cytochrome oxidase subunit one(mtCOI)genes.Pairwise comparisons with mtCOI consensus sequences showed that the majority of these samples had 〈3.5% sequence divergence from groups currently termed Asia I,Asia II-5,Asia II-7,and Asia II-8.The biotype-B B.tabaci from India clustered into the Middle East-Asia Minor 1 group.A new group of B.tabaci from Coimbatore,collected from pumpkin,was related most closely to the Asia I group(6.2% sequence divergence from the consensus Asia I sequence).To increase our understanding of the epidemiology of tomato leaf curl disease(ToLCD)and the key B.tabaci genetic groups involved in virus spread,the indigenous Asia I and the exotic biotype-B population from South India were used to carry out transmission experiments using Tomato leaf curl Bangalore virus(ToLCBV).The acquisition access periods(AAP),inoculation access periods(IAP),latent periods(LP),and ToLCBV transmission efficiencies of the two populations were compared and the biotype-B had the more efficient transmission characteristics.These results are discussed in relation to recent changes in the epidemiology of tomato leaf curl disease in South India. 展开更多
关键词 BEGOMOVIRUSES mitochondrial cytochrome oxidase WHITEFLY Bemisia tabaci virus-vector relationships epidemiology
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Next Generation Transcriptome Sequencing and Quantitative Real-Time PCR Technologies for Characterisation of the Bemisia tabaci Asia 1 mtCOI Phylogenetic Clade 被引量:2
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作者 Susan Seal Mitulkumar V Patel +2 位作者 Carl Collins John Colvin David Bailey 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期281-292,共12页
A programme of functional genomics research is underway at the University of Greenwich,UK,to develop and apply genomics technologies to characterise an economically-important but under-researched Bemisia tabaci(Hemip... A programme of functional genomics research is underway at the University of Greenwich,UK,to develop and apply genomics technologies to characterise an economically-important but under-researched Bemisia tabaci(Hemiptera:Aleyrodidae),the Asia 1 mtCOI phylogenetic group.A comparison of this putative species from India with other important B.tabaci populations and insect species may provide targets for the development of more effective whitefly control strategies.As a first step,next-generation sequencing(NGS)has been used to survey the transcriptome of adult female whitefly,with high quality RNA preparations being used to generate cDNA libraries for NGS using the Roche 454 Titanium DNA sequencing platform.Contig assemblies constructed from the resultant sequences(301 094 reads)using the software program CLC Genomics Workbench generated 3 821 core contigs.Comparison of a selection of these contigs with related sequences from other B.tabaci genetic groups has revealed good alignment for some genes(e.g.,HSP90)but misassemblies in other datasets(e.g.,the vitellogenin gene family),highlighting the need for manual curation as well as collaborative international efforts to obtain accurate assemblies from the existing next generation sequence datasets.Nevertheless,data emerging from the NGS has facilitated the development of accurate and reliable methods for analysing gene expression based on quantitative real-time RT-PCR,illustrating the power of this approach to enable rapid expression analyses in an organism for which a complete genome sequence is currently lacking. 展开更多
关键词 Bemisia tabaci WHITEFLY TRANSCRIPTOME next generation sequencing quantitative real-time (QRT)-PCR Asia 1 mtCOI
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African Cassava Whitefly,Bemisia tabaci,Resistance in African and South American Cassava Genotypes 被引量:1
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作者 Christopher A Omongo Robert Kawuki +5 位作者 Antony C Bellotti Titus Alicai Yona Baguma M N Maruthi Anton Bua John Colvin 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期327-336,共10页
The whitefly,Bemisia tabaci,is a major pest of cassava,particularly in Africa where it is responsible both for the transmission of plant viruses and,increasingly,for direct damage due to feeding by high populations.To... The whitefly,Bemisia tabaci,is a major pest of cassava,particularly in Africa where it is responsible both for the transmission of plant viruses and,increasingly,for direct damage due to feeding by high populations.To date,there have been no practical solutions to combat this emerging problem,due to the inability of the subsistence farmers that grow cassava to afford expensive inputs such as insecticides.A programme of research was carried out linking institutes in Africa,the UK and South America,to identify possible resistance sources in cassava to the whitefly,Bemisia tabaci.The South American genotype MEcu 72 and several Ugandan cassava landraces including Ofumba Chai,Nabwire 1 and Mercury showed good levels of resistance to B.tabaci.Field and screen-house experiments showed that all of the improved,high-yielding cassava mosaic disease(CMD)resistant cassava genotypes assessed were highly susceptible to B.tabaci and supported high populations of all life stages.These data support the hypothesis that the continuing high populations of cassava B.tabaci in Uganda are due,in part,to the widespread adoption of CMD-resistant cassava varieties during the CMD pandemic.They also show that the whitefly,Aleurotrachelus socialis,resistance present in the South American cassava genotypes could have broader applicability in the Old World. 展开更多
关键词 whitefly resistance CASSAVA direct pest Africa South America
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Homopteran Vector Biomarkers for Efficient Circulative Plant Virus Transmission are Conserved in Multiple Aphid Species and the Whitefly Bemisia tabaci 被引量:1
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作者 Michelle Cilia Michael Bereman +2 位作者 Tara Fish Michael J MacCoss stewart Gray 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期249-262,共14页
Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to ... Plant viruses in the families Luteoviridae and Geminiviridae are phloem restricted and are transmitted in a persistent,circulative manner by homopteran insects.Using fluorescence 2-D difference gel electrophoresis to compare the proteomes of F2 genotypes of Schizaphis graminum segregating for virus transmission ability,we recently discovered a panel of protein biomarkers that predict vector competency.Here we used aphid and whitefly nucleotide and expressed sequence tag database mining to test whether these biomarkers are conserved in other homopteran insects.S.graminum gene homologs that shared a high degree of predicted amino acid identity were discovered in two other aphid species and in the whitefly Bemisia tabaci.Selected reaction monitoring mass spectrometry was used to validate the expression of these biomarkers proteins in multiple aphid vector species.The conservation of these proteins in multiple insect taxa that transmit plant viruses along the circulative transmission pathway creates the opportunity to use these biomarkers to rapidly identify insect populations that are the most efficient vectors and allow them to be targeted for control prior to the spread of virus within a crop. 展开更多
关键词 APHID WHITEFLY Schizaphis graminum Bemisia tabaci Acyrthosiphon pisum Toxoptera citricida Myzuspersicae proteomics biomarker circulative transmission BEGOMOVIRUS LUTEOVIRUS GEMINIVIRUS leaflaopper selected reaction monitoring SRM mass spectrometry targeted proteomics Skyline
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Epidemiology of Okra Yellow Vein Mosaic Virus (OYVMV) and Its Management through Tracer, Mycotal and Imidacloprid 被引量:1
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作者 Muhammad Irfan Ali Muhammad Aslam Khan +3 位作者 Abdul Rashid Muhammad Ehetisham-ul-haq Muhammad Talha Javed Muhammad Sajid 《American Journal of Plant Sciences》 2012年第12期1741-1745,共5页
Field trials were conducted to evaluate the epidemiological factors of okra yellow vein mosaic virus (OYVMV). Four varieties of okra were subjected to screening and evaluation of the chemicals. The variety Saloni F1 w... Field trials were conducted to evaluate the epidemiological factors of okra yellow vein mosaic virus (OYVMV). Four varieties of okra were subjected to screening and evaluation of the chemicals. The variety Saloni F1 was highly resistant while Subz Pari was moderately resistant. Diksha was the tolerant variety while Lush Green was moderately susceptible. The chemical Imidacloprid was most effective to control whitefly population and okra yellow vein mosaic virus (OYVMV). Correlation of environmental factors (maximum and minimum temperature, relative humidity and rainfall) with percent plant infection of okra yellow vein mosaic virus (OYVMV) was also determined. There was a significant correlation between environment and disease severity. 展开更多
关键词 EPIDEMIOLOGY IMIDACLOPRID OKRA (Abelmoschus esculentus L. Moench) OKRA YELLOW VEIN Mosaic Virus (OYVMV) and WHITEFLY
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Special Issue Introduction-The Whitefly Bemisia tabaci Species Complex and Begomoviruses:Research Progress and Future Prospects 被引量:1
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作者 Linda L Walling 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第2期171-175,共5页
The whitefly Bemisia tabaci species complex and the begomoviruses they transmit are devastating agricultural pests worldwide.With the rapid and further inva-sions by some members of the whitefly species complex into n... The whitefly Bemisia tabaci species complex and the begomoviruses they transmit are devastating agricultural pests worldwide.With the rapid and further inva-sions by some members of the whitefly species complex into new regions,their impacts on human society are likely to increase in the years to come. 展开更多
关键词 Special Issue Introduction-The Whitefly Bemisia tabaci Species Complex and Begomoviruses
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Resistance to the Whitefly Aleurotrachelus socialis(Hemiptera:Aleyrodidae) and SSR Marker Identification in Advanced Populations of the Hybrid Manihot esculenta subsp.Manihot flabellifolia
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作者 Arturo Carabalí James Montoya-Lerma +2 位作者 Anthony C Belloti Martin Fregene Gerardo Gallego 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第12期2217-2228,共12页
Genes resistant to Aleurotrachelus socialis were transferred to the F 1 from the interspecific hybrid wild species of Manihot flabellifolia to M.esculenta and two advanced generations of backcrosses(BC 1 and BC 2).W... Genes resistant to Aleurotrachelus socialis were transferred to the F 1 from the interspecific hybrid wild species of Manihot flabellifolia to M.esculenta and two advanced generations of backcrosses(BC 1 and BC 2).We characterized the resistance of A.socialis transferred to BC 2 parents(CW67-160,CW67-130,CW67-44),MTAI-8(BC 1),resistant(CMB9B-73) and susceptible(CMB9B-104) genotypes from contrasting pools,and resistant(MEcu-72) and susceptible(CMC-40) genotypes.Whitefly demography and biology were evaluated.SSR molecular markers associated with a phenotypic response of plant resistance were detected in segregating populations(BC 2).Results showed that although female survival time was similar on all hosts,the lowest averages of longevity,fecundity and oviposition rate were observed in the resistant control MEcu- 72,only being significantly similar to the parent CW67-130.When the BC 1 and BC 2 populations were compared,it was found that A.socialis fecundity was eight times lower on CMB9B-73 progeny than on CW67-130,expressing the highest levels of resistance to the whitefly.Ten genotypes of CMB9A and CMB9B family had the best segregation.A total of 486 microsatellite primers were evaluated using bulked segregant analysis(BSA),11 showed polymorphism between the contrasting pools and only one showed significant differences between resistant and susceptible individuals.In conclusion,fecundity was the parameter that impacted most on the intrinsic rate of A.socialis population growth. 展开更多
关键词 CASSAVA wild parents interspecific hybrids whitefly resistance BSA
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Cryptic species composition and genetic diversity within Bemisia tabaci complex in soybean in India revealed by mtCOI DNA sequence
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作者 Prasanna H C Kanakala S +3 位作者 Archana K Jyothsna P Varma R K Malathi V G 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第9期1786-1795,共10页
Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving ... Bemisia tabaci is a cryptic species complex, causing signiifcant loss on many agricultural y important crops worldwide. Knowledge on species composition and diversity within B. tabaci complex is critical for evolving sustainable pest management strategies. Here we investigate the whitelfy species complex in soybean in major soybean growing states of India. The mitochondrial cytochrome oxidase gene subunit-1 (mtCOI) based phylogenetic relationships established using Bayesian methods indicated the existence of three cryptic species namely Asia I, Asia II 1, and Asia II 7. Al the haplotypes detected in the study could be assigned to these three cryptic species fol owing the species demarcation criteria of 3.5%divergence threshold. Of these, Asia II 1 was found to be predominant with wide spread distribution across the surveyed regions from cool temperate zones to hot and humid tropical plains. On the contrary, cryptic species Asia II 7 showed localized distribu-tion. The Asia II 1 exhibited the highest haplotype diversity and Asia I showed high level of nucleotide diversity. There was a signiifcantly high genetic differentiation among these three cryptic species. The MEAM 1, a dreadful invasive species was not detected in the specimens tested in the current study. The diversity and distribution of three cryptic species is discussed in the light of current knowledge on distribution of whitelfy species in India and yel ow mosaic disease observed during sampling survey. 展开更多
关键词 WHITEFLY mitochondrial cytochrome oxidase gene subunit-1 AsiaⅠ AsiaⅡ 1 AsiaⅡ 7 begomovirus and yellow mosaic disease
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Multiple Cassava Viruses’ Co-Infections and Resurgence of Pests Are Leading to Severe Symptoms and Yield Losses on Cassava in the South-Kivu Region, Democratic Republic of Congo
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作者 E. B. Bisimwa D. R. Birindwa +4 位作者 M. O. Yomeni N. Rudahaba K. Byamungu S. Winter C. Bragard 《American Journal of Plant Sciences》 2019年第11期1969-1988,共20页
A survey was completed during the last five years (2014-2018) to update and assess changes in the cassava pests and diseases occurrence as well as to understand how these changes occur regarding the recent new viruses... A survey was completed during the last five years (2014-2018) to update and assess changes in the cassava pests and diseases occurrence as well as to understand how these changes occur regarding the recent new viruses spread under a climate change context in the South-Kivu region, Democratic Republic of Congo. The eight territories of South-Kivu were surveyed during the rainy seasons from 2015 until 2017 except for Uvira and Fizi where data were recorded in 2018 regarding the prevalence of unstable agro ecological factors which may influence pest and diseases dynamic in these two territories. During the data collection, pests and disease incidence and severity were recorded as well as the sampling of cassava leaves for molecular viruses’ detection. The recorded data showed that Cassava mosaic disease (CMD) remains the most encountered disease (75% - 80%) and its vector (the whitefly Bemisia tabaci) the most important pest (10 - 18 adult whiteflies per leaf). The major changes were recorded for Cassava brown streak disease, recently reported in the region, for which a significant increase was recorded, with not reports before 2012 to 70% - 80% incidence in 2018 in the Ruzizi plain area (Uvira and Fizi). Molecular detection revealed a coexistence of both two viruses, the cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) in single and mixed infections. Co-infections cassava mosaic and cassava brown streak diseases were frequently recorded resulting in severe expression leaves’ symptoms and tuber yields’ losses. Common pests and diseases considered as minor have drastically become serious threat to cassava production by increasing both their incidence and severity values. The cassava bacterial blight (20%), cassava green mite (20% - 70%), cassava mealy bugs and cassava grasshoppers were recorded as a great challenge in relation with an occurrence of long dry season resulting in four consecutive dry months without rain. The high occurrence of CBSD on new CMD-resistant varieties has enforced farmers to recover local CMD-susceptible varieties which revealed a relative CBSD-tolerance and moderate yield losses. This need for virus control is really challenging cassava crop in a region where the diseases pressure is high and people consume cassava and its products seven days a week. Research gaps identified in the study are the need for breeding new varieties with tolerance/resistance to both CMD and CBSD viruses as well as the extensive use of rapid multiplex diagnostic tools to ensure early diagnostic and availability of good quality planting material at farmers’ level. 展开更多
关键词 CASSAVA GENOTYPES Distribution Climate Factors South-Kivu Re-Emergence WHITEFLY
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Contribution to the knowledge of Tetraleurodes Cockerell(Hemiptera: Aleyrodidae) in China
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作者 Jirui WANG Yuzhou DU Zhihong XU 《Entomotaxonomia》 CSCD 2022年第1期13-23,共11页
Five species in the genus Tetraleurodes Cockerell(Hemiptera:Aleyrodidae)known to occur in China were studied.Tetraleurodes thenmozhiae Jesudasan and David is recognized as a newly recorded species to China and is rede... Five species in the genus Tetraleurodes Cockerell(Hemiptera:Aleyrodidae)known to occur in China were studied.Tetraleurodes thenmozhiae Jesudasan and David is recognized as a newly recorded species to China and is redescribed with morphological illustrations and photographs taken with a scanning electron microscope(SEM).Comments on the other four species,T.acaciae(Quaintance),T.neemani Bink-Moenen,T.graminis Takahashi and T.oplismeni Takahashi are provided.An identification key to the puparia of Chinese Tetraleurodes species is given.Specimens are deposited in the Insect Collection of Zhejiang Agriculture&Forestry University,Hangzhou,China. 展开更多
关键词 WHITEFLY TAXONOMY KEY
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