Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and...Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and protecting public health.However,handling complex samples such as human and wastewater can be challenging due to their dynamic and complex composition and the ultralow concentration of target analytes.This review presents a comprehensive overview of the latest breakthroughs in waterborne virus biosensors.It begins by highlighting several promising strategies that enhance the sensing performance of optical and electrochemical biosensors in human samples.These strategies include optimizing bioreceptor selection,transduction elements,signal amplification,and integrated sensing systems.Furthermore,the insights gained from biosensing waterborne viruses in human samples are applied to improve biosensing in wastewater,with a particular focus on sampling and sample pretreatment due to the dispersion characteristics of waterborne viruses in wastewater.This review suggests that implementing a comprehensive system that integrates the entire waterborne virus detection process with high-accuracy analysis could enhance virus monitoring.These findings provide valuable insights for improving the effectiveness of waterborne virus detection,which could have significant implications for public health and environmental management.展开更多
We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relat...We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relationship between biosensors. A parallel study of biosensors (edible cucumber slices) had also been conducted, and we found that the circadian rhythm of gas concentrations emitted from biosensors changes seasonally. The pyramid power and Bio-Entanglement did not change the number of cycles in the periodic approximation curve representing circadian rhythm. Therefore, in this paper we analyzed the influence of the pyramid power and Bio-Entanglement, i.e., their influence on the phase, amplitude, and correlation coefficient of the periodic approximation curve representing the circadian rhythm of emitted gas concentrations. The main results are as follows. 1) The pyramid power shifted the phase of the periodic approximation curve representing the circadian rhythm by 43 minutes. 2) The amplitude of the periodic approximation curve changed with the pyramid power and the Bio-Entanglement. The effect on the lower and upper sections of the biosensors stacked in two layers was different, with a tendency to increase the amplitude of the lower layer and decrease the amplitude of the upper layer. 3) The pyramid power and the Bio-Entanglement affected the correlation coefficient between gas concentration and the periodic approximation curve representing the circadian rhythm of gas concentration. The effect on the lower and upper layers of the biosensors was different, with a tendency for the lower layer correlation coefficient to be larger and the upper layer correlation coefficient to be smaller. Previously we demonstrated that the pyramid power and the Bio-Entanglement affect the ratio of gas concentration, i.e., psi index Ψ. In this paper we demonstrate for the first time that the pyramid power and the Bio-Entanglement affect time, i.e., phase difference.展开更多
Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures...Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures.Rapid,sensitive,efficient and portable detection method is highly essential for fish viral diseases detection.Biosensor strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and selectivity.With the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids fisheries.This paper reviews the current detection development of salmonids viral diseases.The present limitations and challenges of salmonids virus diseases surveillance and early detection are presented.Novel nucleic acid semiconductor biosensors are briefly reviewed.The perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.展开更多
The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the pr...The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.展开更多
With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monit...With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monitoring,and pre-diagnostics.This paper reviews the recent progress in sweat biosensors and sensing systems integrated into textiles for wearable body status monitoring.The mechanisms of biosensors that are commonly adopted for biomarkers analysis are first introduced.The classification,fabrication methods,and applications of textile conductors in different configurations and dimensions are then summarized.Afterward,innovative strategies to achieve efficient sweat collection with textile-based sensing patches are presented,followed by an in-depth discussion on nanoengineering and system integration approaches for the enhancement of sensing performance.Finally,the challenges of textile-based sweat sensing devices associated with the device reusability,washability,stability,and fabrication reproducibility are discussed from the perspective of their practical applications in wearable healthcare.展开更多
Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and ...Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.展开更多
In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosenso...In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosensor.ZnO nanoparticles have been prepared via the green route using olive leaves extract as a reductant.ZnO/Ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole(Py)monomer using ferric chloride(FeCl3)as an oxidizing agent.The produced materials and the composite films were characterized using X-ray diffraction analysis(XRD),scanning electron microscope(SEM),Fourier transform infrared(FTIR)and thermogravimetric analysis(TGA).Glucose oxidase was successfully immobilized on the surface of the prepared film and then ZnO/Ppy/CA/GOx composite was sputtered with platinum electrode for the current determination at different initial concentrations of glucose.Current measurements proved the suitability and the high sensitivity of the constructed biosensor for the detection of glucose levels in different samples.The performance of the prepared biosensor has been assessed by measuring and comparing glucose concentrations up to 800 ppm.The results affirmed the reliability of the developed biosensor towards real samples which suggests the wide-scale application of the proposed biosensor.展开更多
A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical resea...A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical research,for example,the blood glucose biosensor.This review focuses on the current state of biosensor research with respect to efficient,specific and rapid detection of hepatitis B virus(HBV).The biosensors developed based on different techniques,including optical methods(e.g.,surface plasmon resonance),acoustic wave technologies(e.g.,quartz crystal microbalance),electrochemistry(amperometry,voltammetry and impedance) and novel nanotechnology,are also discussed.展开更多
Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this...Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery,biopharmaceutical manufacturing,gene therapy and tissue engineering,etc.In this review,we focus on current technologies of synthetic biosensors for disease detection.We start with the design principle of synthetic biosensors.Then we move towards the characteristics of simple synthetic biosensors,which can respond to a single input signal,and complex synthetic biosensors including Boolean gate biosensors,cascade biosensors,time-delay biosensors,oscillator biosensors and hysteretic biosensors,which can respond to more than two input signals and perform complex tasks.Synthetic biosensor has showed great potential in disease detection,but it is still in its infancy stage.More efforts should be made in identifying and constructing clinically relevant regulation systems.Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor.The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene-and cell-based therapies.展开更多
Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for p...Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for pesticide detection. Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. Artificial Neural Networks, that allow the analysis of pesticide mixtures are also presented. Recent advances in the field of nanomaterials merit special mention. The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides.展开更多
In last few decades there is exponential increase in use of organophosphorus (OP) compounds as pesticides and insecticides leading to adverse effect on human population and live stock. There is a great need to develop...In last few decades there is exponential increase in use of organophosphorus (OP) compounds as pesticides and insecticides leading to adverse effect on human population and live stock. There is a great need to develop portable analytical tools that are amenable for remediation and bioremediation process monitoring, where rapid analysis of large number of samples is essential. Determination of various organophosphorus compounds has been achieved by integrating biocomponents with different transducers. The close integration of the biological events with the generation of a signal offers the potential for fabricating compact and easy-to-use analytical tools of high sensitivity and specificity. With the availability of new materials, associated with new sensing techniques has led to remarkable innovations in the design and construction of organophosphorus biosensors. The present review describes the specifications of most of the electrochemical Organophosphorus biosensors reported till date.展开更多
The results of comparative theoretical analyzes of the behavior of internal low-frequency noises, signal-to-noise ratio and sensitivity to DNA molecules for EIS and ISFET based nanosize biosensors are presented. It is...The results of comparative theoretical analyzes of the behavior of internal low-frequency noises, signal-to-noise ratio and sensitivity to DNA molecules for EIS and ISFET based nanosize biosensors are presented. It is shown that EIS biosensor is more sensitive to the presence of DNA molecules in aqueous solution than ISFET sensor. Internal electrical noises level decreases with the increase of concentration of DNA molecules in aqueous solution. In the frequency range 10−3 - 103 Hz noises level for EIS sensor about in three orders is higher than for ISFET sensor. In the other hand, signal-to-noise ratio for capacitive EIS biosensor is much higher than for ISFET sensor.展开更多
Since October 2007, we have been conducting rigorous scientific experiments to elucidate the so-called “pyramid power”. The experiments use a pyramidal structure (PS). In order to detect the PS effects, a biosensor ...Since October 2007, we have been conducting rigorous scientific experiments to elucidate the so-called “pyramid power”. The experiments use a pyramidal structure (PS). In order to detect the PS effects, a biosensor is made from cucumber fruit sections and the released volatile components are analyzed as gas concentration. We reported the phenomenon of entanglement between biosensors in part IV of the paper series, Potential Power of the Pyramidal Structure. The entanglement affected the gas concentration of the biosensors 8 m away, but not the biosensors at the PS apex. In this paper, we report another characteristic of the entanglement. The results and our conclusion are as follows. Result 1: The periodicity of diurnal variation in gas concentration changed with entanglement. Result 2: As a result of analyzing the gas concentration data separately for the four seasons of winter, spring, summer, and autumn, the seasonal dependence of the periodicity of diurnal variation due to entanglement was clarified. Conclusion: We reaffirmed the existence of the entanglement between biosensors due to the pyramid effects by a phenomenon different from Part IV of the paper series, Potential Power of the Pyramidal Structure. We expect that our research results will be widely accepted in the future and will become the foundation for a new research field in science, with a wide range of applications.展开更多
Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applica...Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applications of advanced nanomaterials in biosensors.Every year there are several advances in sensing techniques that can be attributed to nanomaterials,biorecognition elements,or their related fabrication techniques.The further development of nanotechnology-based sensors provides a wide variety of opportunities to modern research.Advanced nanomaterials can provide remarkable optical,electrical,mechanical,and catalytic properties.For example,transition metals and organic polymers have been used in the fabrication of powerful,sensitive,and precise biosensors.The distinctive properties of advanced nanomaterials have been widely incorporated into biosensors.However,fabrication techniques also play important roles in the development of these devices.Therefore,we present a review of some of the advanced nanomaterials that have been widely used over the last few years and discuss their fabrication techniques.The focus of this review is to provide a directional perspective of recently fabricated advanced nanomaterial-based biosensors in the diagnosis of various diseases.展开更多
Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials ...Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials to optimize biosensor design. The attachment of peptides and proteins to nanocrystalline cellulose and their proven retention of activity provide a route to bioactive conjugates useful in designs for point of care biosensors. Elastase is a biomarker for a number of inflammatory diseases including chronic wounds, and its rapid sensitive detection with a facile approach to sensing is of interest. An increased interest in the use of elastase sensors for point of care diagnosis is resulting in a variety of approaches to elsastase sensors utilizing different detection technologies. Here elastase substrate peptide-celluose conjugates synthesized as colorimetric and fluorescent sensors on cotton cellulose nanocrystals are compared. The structure of the sensor peptide-nanocellulose crystals when modeled with computational crystal structure parameters demonstrates the spatio-stoichiometric features of the nanocrystalline surface that allows ligand to active site protease interacttion. An understanding of the structure/function relations of enzyme and conjugate substrate of the peptides covalently attached to nancellulose has implications for enhancing the biomolecular transducer. The potential applications of both fluorescent and colorimetric detection to markers like elastase using peptide cotton cellulose nanocrystals as a transducer surface to model point of care biosensors for protease detection are discussed.展开更多
Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent ...Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent feed contamination.Traditional detection methods can no longer meet the needs of massive,real-time,simple,and fast mycotoxin monitoring.Rapid detection methods based on advanced material and sensor technology are the future trend.In this review,we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods,especially for simultaneous multiplex mycotoxin determination.Immunoassays,biosensors,and the prominent roles of nanomaterials are introduced.The principles of different types of recognition and signal transduction are explained,and the merits and pitfalls of these methods are compared.Furthermore,limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.展开更多
Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites ...Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites pose a potential risk of allergic reactions,bacterial resistance,and increased cancer incidence.Residual antibiotics and the resulting bacterial antibiotic resistance have been recognized as a global challenge that has attracted increasing attention.Therefore,monitoring antibiotics is a critical way to limit the ecological risks from antibiotic pollution.Accordingly,it is desirable to devise new analytical platforms to achieve efficient antibiotic detection with excellent sensitivity and specificity.Quantum dots(QDs)are regarded as an ideal material for use in the development of antibiotic detection biosensors.In this review,we characterize different types of QDs,such as silicon,chalcogenide,carbon,and other doped QDs,and summarize the trends in QD-based antibiotic detection.QD-based sensing applications are classified according to their recognition strategies,including molecularly imprinted polymers(MIPs),aptamers,and immunosensors.We discuss the advantages of QD-derived antibiotic sensors,including low cost,good sensitivity,excellent stability,and fast response,and illustrate the current challenges in this field.展开更多
Non-enzymatic biosensors based on mixed transition metal oxides are deemed as the most promising devices due to their high sensitivity,selectivity,wide concentration range,low detection limits,and excellent recyclabil...Non-enzymatic biosensors based on mixed transition metal oxides are deemed as the most promising devices due to their high sensitivity,selectivity,wide concentration range,low detection limits,and excellent recyclability.Spinel NiCo2O4 mixed oxides have drawn considerable attention recently due to their outstanding advantages including large specific surface area,high permeability,short electron,and ion diffusion pathways.Because of the rapid development of non-enzyme biosensors,the current state of methods for synthesis of pure and composite/hybrid NiCo2P4 materials and their subsequent electrochemical biosensing applications are systematically and comprehensively reviewed herein.Comparative analysis reveals better electrochemical sensing of bioanalytes by one-dimensional and two-dimensional NiCo2O4 nano-/microstructures than other morphologies.Better biosensing efficiency of NiCo2O4 as compared to corresponding individual metal oxides,viz.NiO and Co3O4,is attributed to the close intrinsic-state redox couples of Ni3+/Ni2+(0.58 V/0.49 V) and Co3+/Co2+(0.53 V/0.51 V).Biosensing performance of NiCo2O4 is also significantly improved by making the composites of NiCo2O4 with conducting carbonaceous materials like graphene,reduced graphene oxide,carbon nanotubes(single and multi-walled),carbon nanofibers;conducting polymers like polypyrrole(PPy),polyaniline(PANI);metal oxides NiO,Co3O4,SnO2,MnO2;and metals like Au,Pd,etc.Various factors affecting the morphologies and biosensing parameters of the nano-/microstructured NiCo2O4 are also highlighted.Finally,some drawbacks and future perspectives related to this promising field are outlined.展开更多
As a new kind of analytical instrument,the principles,types and characters of biosensors were discussed in this paper.A biosensor is usually composed of a recognition element of biological origin and a physicochemical...As a new kind of analytical instrument,the principles,types and characters of biosensors were discussed in this paper.A biosensor is usually composed of a recognition element of biological origin and a physicochemical transducer.The biological element is capable of sensing the presence,activity or concentration of a chemical analyse in solution.The sensing takes place either as a binding event or a biocatalytical event.These interactions produce a measurable change in a solution property,in which the transducer is converted into a quantifiable electrical signal.The principles,types and applications of biosensors in environmental inspection,food production,clinical medicine and military defense were reviewed,and the trends in research were predicated.Furthermore,an attempt had been made to describe the future development directions and prospects.展开更多
In the past two decades,the biological and medical fields have seen great advances in the development of biosensors and bioehips capable of characterizing and quantifying biomolecules.This lecture is meant to discuss ...In the past two decades,the biological and medical fields have seen great advances in the development of biosensors and bioehips capable of characterizing and quantifying biomolecules.This lecture is meant to discuss the development and applications of advanced electroanalysis,biophotonics,nanotechnology,MEMS- based biosensors and biochips for biomedical diagnostics and physical performances of athlete.展开更多
基金supported by the Research Center for Industries of the Future of Westlake University,China(Grant No.:210230006022219/001)the National Natural Science Foundation of China(Grant No.:82104122)+1 种基金Westlake University,China(Grant No.:10318A992001)the Leading Innovative and Entrepreneur Team Introduction Program of Zhejiang,China(Grant No.:2020R01005).
文摘Waterborne viruses that can be harmful to human health pose significant challenges globally,affecting health care systems and the economy.Identifying these waterborne pathogens is essential for preventing diseases and protecting public health.However,handling complex samples such as human and wastewater can be challenging due to their dynamic and complex composition and the ultralow concentration of target analytes.This review presents a comprehensive overview of the latest breakthroughs in waterborne virus biosensors.It begins by highlighting several promising strategies that enhance the sensing performance of optical and electrochemical biosensors in human samples.These strategies include optimizing bioreceptor selection,transduction elements,signal amplification,and integrated sensing systems.Furthermore,the insights gained from biosensing waterborne viruses in human samples are applied to improve biosensing in wastewater,with a particular focus on sampling and sample pretreatment due to the dispersion characteristics of waterborne viruses in wastewater.This review suggests that implementing a comprehensive system that integrates the entire waterborne virus detection process with high-accuracy analysis could enhance virus monitoring.These findings provide valuable insights for improving the effectiveness of waterborne virus detection,which could have significant implications for public health and environmental management.
文摘We have demonstrated the existence of a pyramid power and have revealed its characteristics by strictly scientific experiments using biosensors. We also revealed the existence of a Bio-Entanglement, an entangled relationship between biosensors. A parallel study of biosensors (edible cucumber slices) had also been conducted, and we found that the circadian rhythm of gas concentrations emitted from biosensors changes seasonally. The pyramid power and Bio-Entanglement did not change the number of cycles in the periodic approximation curve representing circadian rhythm. Therefore, in this paper we analyzed the influence of the pyramid power and Bio-Entanglement, i.e., their influence on the phase, amplitude, and correlation coefficient of the periodic approximation curve representing the circadian rhythm of emitted gas concentrations. The main results are as follows. 1) The pyramid power shifted the phase of the periodic approximation curve representing the circadian rhythm by 43 minutes. 2) The amplitude of the periodic approximation curve changed with the pyramid power and the Bio-Entanglement. The effect on the lower and upper sections of the biosensors stacked in two layers was different, with a tendency to increase the amplitude of the lower layer and decrease the amplitude of the upper layer. 3) The pyramid power and the Bio-Entanglement affected the correlation coefficient between gas concentration and the periodic approximation curve representing the circadian rhythm of gas concentration. The effect on the lower and upper layers of the biosensors was different, with a tendency for the lower layer correlation coefficient to be larger and the upper layer correlation coefficient to be smaller. Previously we demonstrated that the pyramid power and the Bio-Entanglement affect the ratio of gas concentration, i.e., psi index Ψ. In this paper we demonstrate for the first time that the pyramid power and the Bio-Entanglement affect time, i.e., phase difference.
基金supported by the National Key Research and Development Program of China(2022YFC2601304)National Key Research and Development Program of China(2022YFC2602100)。
文摘Viral diseases represent one of the major threats for salmonids aquaculture.Early detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control measures.Rapid,sensitive,efficient and portable detection method is highly essential for fish viral diseases detection.Biosensor strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and selectivity.With the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids fisheries.This paper reviews the current detection development of salmonids viral diseases.The present limitations and challenges of salmonids virus diseases surveillance and early detection are presented.Novel nucleic acid semiconductor biosensors are briefly reviewed.The perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.
基金supported by the National Key Research and Development Program of China(2022YFC2602100)supported by National key research and development program(2021YFC2600602)。
文摘The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.
基金supported by the National Natural Science Foundation of China(62201243)Fundamental and Applied Research Grant of Guangdong Province(2021A1515110627)+3 种基金Southern University of Science and Technology(Y01796108,Y01796208)RGC Senior Research Fellow Scheme of Hong Kong(SRFS2122-5S04)the Hong Kong Polytechnic University(1-ZVQM),RI-Wear of PolyU(1-CD44)Shenzhen Science and Technology Innovation Committee(SGDX20210823103403033).
文摘With the rapid technological innovation in materials engineering and device integration,a wide variety of textilebased wearable biosensors have emerged as promising platforms for personalized healthcare,exercise monitoring,and pre-diagnostics.This paper reviews the recent progress in sweat biosensors and sensing systems integrated into textiles for wearable body status monitoring.The mechanisms of biosensors that are commonly adopted for biomarkers analysis are first introduced.The classification,fabrication methods,and applications of textile conductors in different configurations and dimensions are then summarized.Afterward,innovative strategies to achieve efficient sweat collection with textile-based sensing patches are presented,followed by an in-depth discussion on nanoengineering and system integration approaches for the enhancement of sensing performance.Finally,the challenges of textile-based sweat sensing devices associated with the device reusability,washability,stability,and fabrication reproducibility are discussed from the perspective of their practical applications in wearable healthcare.
基金the National Natural Science Foundation of China(Grant No.21705087)Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province(096-1622002)+2 种基金Research Foundation for Distinguished Scholars of Qingdao Agricultural University(663-1117015)the Postgraduate Innovation Program of Qingdao Agricultural University(QNYCX21069)the National Innovation Training Program for College Students(No.202210435030).
文摘Microbial cell surface display technology is a recombinant technology to express target proteins on the cell membrane,which can be used to redesign the cell surface with functional proteins and peptides.Bacterial and yeast surface display systems are the most common cell surface display systems of prokaryotic and eukaryotic proteins,that are widely applied as the core elements in the field of biosensors due to their advantages,including enhanced stability,high yield,good safety,expression of larger and more complex proteins.To further promote the performance of biosensors,the biomineralized microbial surface display technology was proposed.This review summarized the different microbial surface display systems and the biomineralized surface display systems,where the mechanisms of surface display and biomineralization were introduced.Then we described the recent progress of their applications on biosensors for different types of detection targets.Finally,the outlooks and tendencies were discussed and forecasted with the expectation to provide some general functions and enlightenments to this aspect of research.
文摘In this study,green zinc oxide(ZnO)/polypyrrole(Ppy)/cellulose acetate(CA)film has been synthesized via solvent casting.This film was used as supporting material for glucose oxidase(GOx)to sensitize a glucose biosensor.ZnO nanoparticles have been prepared via the green route using olive leaves extract as a reductant.ZnO/Ppy nanocomposite has been synthesized by a simple in-situ chemical oxidative polymerization of pyrrole(Py)monomer using ferric chloride(FeCl3)as an oxidizing agent.The produced materials and the composite films were characterized using X-ray diffraction analysis(XRD),scanning electron microscope(SEM),Fourier transform infrared(FTIR)and thermogravimetric analysis(TGA).Glucose oxidase was successfully immobilized on the surface of the prepared film and then ZnO/Ppy/CA/GOx composite was sputtered with platinum electrode for the current determination at different initial concentrations of glucose.Current measurements proved the suitability and the high sensitivity of the constructed biosensor for the detection of glucose levels in different samples.The performance of the prepared biosensor has been assessed by measuring and comparing glucose concentrations up to 800 ppm.The results affirmed the reliability of the developed biosensor towards real samples which suggests the wide-scale application of the proposed biosensor.
基金Supported by National Natural Science Foundation of China,No.81371885
文摘A biosensor is an analytical device used for the detection of analytes,which combines a biological component with a physicochemical detector.Recently,an increasing number of biosensors have been used in clinical research,for example,the blood glucose biosensor.This review focuses on the current state of biosensor research with respect to efficient,specific and rapid detection of hepatitis B virus(HBV).The biosensors developed based on different techniques,including optical methods(e.g.,surface plasmon resonance),acoustic wave technologies(e.g.,quartz crystal microbalance),electrochemistry(amperometry,voltammetry and impedance) and novel nanotechnology,are also discussed.
基金Fund supported by the National Natural Science Foundation of China (81770491) and the Ministry of Education Innovation TeamDevelopment Program of China (IRT16R57).
文摘Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them.By overexpressing,blocking and rewiring native gene pathways,synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery,biopharmaceutical manufacturing,gene therapy and tissue engineering,etc.In this review,we focus on current technologies of synthetic biosensors for disease detection.We start with the design principle of synthetic biosensors.Then we move towards the characteristics of simple synthetic biosensors,which can respond to a single input signal,and complex synthetic biosensors including Boolean gate biosensors,cascade biosensors,time-delay biosensors,oscillator biosensors and hysteretic biosensors,which can respond to more than two input signals and perform complex tasks.Synthetic biosensor has showed great potential in disease detection,but it is still in its infancy stage.More efforts should be made in identifying and constructing clinically relevant regulation systems.Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor.The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene-and cell-based therapies.
文摘Due to the large amounts of pesticides commonly used and their impact on health, prompt and accurate pesticide analysis is important. This review gives an overview of recent advances and new trends in biosensors for pesticide detection. Optical, electrochemical and piezoelectric biosensors have been reported based on the detection method. In this review biosensors have been classified according to the immobilized biorecognition element: enzymes, cells, antibodies and, more rarely, DNA. The use of tailor-designed biomolecules, such as aptamers and molecularly imprinted polymers, is reviewed. Artificial Neural Networks, that allow the analysis of pesticide mixtures are also presented. Recent advances in the field of nanomaterials merit special mention. The incorporation of nanomaterials provides highly sensitive sensing devices allowing the efficient detection of pesticides.
文摘In last few decades there is exponential increase in use of organophosphorus (OP) compounds as pesticides and insecticides leading to adverse effect on human population and live stock. There is a great need to develop portable analytical tools that are amenable for remediation and bioremediation process monitoring, where rapid analysis of large number of samples is essential. Determination of various organophosphorus compounds has been achieved by integrating biocomponents with different transducers. The close integration of the biological events with the generation of a signal offers the potential for fabricating compact and easy-to-use analytical tools of high sensitivity and specificity. With the availability of new materials, associated with new sensing techniques has led to remarkable innovations in the design and construction of organophosphorus biosensors. The present review describes the specifications of most of the electrochemical Organophosphorus biosensors reported till date.
文摘The results of comparative theoretical analyzes of the behavior of internal low-frequency noises, signal-to-noise ratio and sensitivity to DNA molecules for EIS and ISFET based nanosize biosensors are presented. It is shown that EIS biosensor is more sensitive to the presence of DNA molecules in aqueous solution than ISFET sensor. Internal electrical noises level decreases with the increase of concentration of DNA molecules in aqueous solution. In the frequency range 10−3 - 103 Hz noises level for EIS sensor about in three orders is higher than for ISFET sensor. In the other hand, signal-to-noise ratio for capacitive EIS biosensor is much higher than for ISFET sensor.
文摘Since October 2007, we have been conducting rigorous scientific experiments to elucidate the so-called “pyramid power”. The experiments use a pyramidal structure (PS). In order to detect the PS effects, a biosensor is made from cucumber fruit sections and the released volatile components are analyzed as gas concentration. We reported the phenomenon of entanglement between biosensors in part IV of the paper series, Potential Power of the Pyramidal Structure. The entanglement affected the gas concentration of the biosensors 8 m away, but not the biosensors at the PS apex. In this paper, we report another characteristic of the entanglement. The results and our conclusion are as follows. Result 1: The periodicity of diurnal variation in gas concentration changed with entanglement. Result 2: As a result of analyzing the gas concentration data separately for the four seasons of winter, spring, summer, and autumn, the seasonal dependence of the periodicity of diurnal variation due to entanglement was clarified. Conclusion: We reaffirmed the existence of the entanglement between biosensors due to the pyramid effects by a phenomenon different from Part IV of the paper series, Potential Power of the Pyramidal Structure. We expect that our research results will be widely accepted in the future and will become the foundation for a new research field in science, with a wide range of applications.
基金This work was supported by the Department of Science&Technology(DST)(Grant No.TDP/BDTD/33/2019)the Science and Engineering Research Board(SERB)(Grant Nos.EMR/2016/007564 and YSS/2015/000023)the Biotechnology Industry Research Assistance Council(BIRAC)(Grant No.BT/IIPME0211/02/16).
文摘Biosensors have acquired much importance in drug discovery,medical diagnostics,food safety,defense,security,and monitoring of environmental conditions.Furthermore,there has been great progress in the potential applications of advanced nanomaterials in biosensors.Every year there are several advances in sensing techniques that can be attributed to nanomaterials,biorecognition elements,or their related fabrication techniques.The further development of nanotechnology-based sensors provides a wide variety of opportunities to modern research.Advanced nanomaterials can provide remarkable optical,electrical,mechanical,and catalytic properties.For example,transition metals and organic polymers have been used in the fabrication of powerful,sensitive,and precise biosensors.The distinctive properties of advanced nanomaterials have been widely incorporated into biosensors.However,fabrication techniques also play important roles in the development of these devices.Therefore,we present a review of some of the advanced nanomaterials that have been widely used over the last few years and discuss their fabrication techniques.The focus of this review is to provide a directional perspective of recently fabricated advanced nanomaterial-based biosensors in the diagnosis of various diseases.
文摘Nanocrystalline cellulose is an amphiphilic, high surface area material that can be easily functionalized and is biocompatible and eco-friendly. It has been used singularly and in combination with other nanomaterials to optimize biosensor design. The attachment of peptides and proteins to nanocrystalline cellulose and their proven retention of activity provide a route to bioactive conjugates useful in designs for point of care biosensors. Elastase is a biomarker for a number of inflammatory diseases including chronic wounds, and its rapid sensitive detection with a facile approach to sensing is of interest. An increased interest in the use of elastase sensors for point of care diagnosis is resulting in a variety of approaches to elsastase sensors utilizing different detection technologies. Here elastase substrate peptide-celluose conjugates synthesized as colorimetric and fluorescent sensors on cotton cellulose nanocrystals are compared. The structure of the sensor peptide-nanocellulose crystals when modeled with computational crystal structure parameters demonstrates the spatio-stoichiometric features of the nanocrystalline surface that allows ligand to active site protease interacttion. An understanding of the structure/function relations of enzyme and conjugate substrate of the peptides covalently attached to nancellulose has implications for enhancing the biomolecular transducer. The potential applications of both fluorescent and colorimetric detection to markers like elastase using peptide cotton cellulose nanocrystals as a transducer surface to model point of care biosensors for protease detection are discussed.
基金The financial support from the National Key Research and Development Program of China(2017YFC1600300).
文摘Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent feed contamination.Traditional detection methods can no longer meet the needs of massive,real-time,simple,and fast mycotoxin monitoring.Rapid detection methods based on advanced material and sensor technology are the future trend.In this review,we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods,especially for simultaneous multiplex mycotoxin determination.Immunoassays,biosensors,and the prominent roles of nanomaterials are introduced.The principles of different types of recognition and signal transduction are explained,and the merits and pitfalls of these methods are compared.Furthermore,limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.
基金supported by grants from the National Key Research and Development Project(Project No.:2019YFC1605800)the National Natural Science Foundation of China(Grant Nos.:22006070 and 82103816)+2 种基金the Natural Science Foundation of Jiangsu Province(Grant Nos.:BK20200715,BK20200718,and BK20210538)the Natural Science Foundation of Jiangsu Higher Education Institutions,China(Grant No.:20KJB350010)the Natural Science Fund for Colleges and Universities in Jiangsu Province(Grant No.:19KJB530011).
文摘Antibiotics are a category of chemical compounds used to treat bacterial infections and are widely applied in cultivation,animal husbandry,aquaculture,and pharmacy.Currently,residual antibiotics and their metabolites pose a potential risk of allergic reactions,bacterial resistance,and increased cancer incidence.Residual antibiotics and the resulting bacterial antibiotic resistance have been recognized as a global challenge that has attracted increasing attention.Therefore,monitoring antibiotics is a critical way to limit the ecological risks from antibiotic pollution.Accordingly,it is desirable to devise new analytical platforms to achieve efficient antibiotic detection with excellent sensitivity and specificity.Quantum dots(QDs)are regarded as an ideal material for use in the development of antibiotic detection biosensors.In this review,we characterize different types of QDs,such as silicon,chalcogenide,carbon,and other doped QDs,and summarize the trends in QD-based antibiotic detection.QD-based sensing applications are classified according to their recognition strategies,including molecularly imprinted polymers(MIPs),aptamers,and immunosensors.We discuss the advantages of QD-derived antibiotic sensors,including low cost,good sensitivity,excellent stability,and fast response,and illustrate the current challenges in this field.
文摘Non-enzymatic biosensors based on mixed transition metal oxides are deemed as the most promising devices due to their high sensitivity,selectivity,wide concentration range,low detection limits,and excellent recyclability.Spinel NiCo2O4 mixed oxides have drawn considerable attention recently due to their outstanding advantages including large specific surface area,high permeability,short electron,and ion diffusion pathways.Because of the rapid development of non-enzyme biosensors,the current state of methods for synthesis of pure and composite/hybrid NiCo2P4 materials and their subsequent electrochemical biosensing applications are systematically and comprehensively reviewed herein.Comparative analysis reveals better electrochemical sensing of bioanalytes by one-dimensional and two-dimensional NiCo2O4 nano-/microstructures than other morphologies.Better biosensing efficiency of NiCo2O4 as compared to corresponding individual metal oxides,viz.NiO and Co3O4,is attributed to the close intrinsic-state redox couples of Ni3+/Ni2+(0.58 V/0.49 V) and Co3+/Co2+(0.53 V/0.51 V).Biosensing performance of NiCo2O4 is also significantly improved by making the composites of NiCo2O4 with conducting carbonaceous materials like graphene,reduced graphene oxide,carbon nanotubes(single and multi-walled),carbon nanofibers;conducting polymers like polypyrrole(PPy),polyaniline(PANI);metal oxides NiO,Co3O4,SnO2,MnO2;and metals like Au,Pd,etc.Various factors affecting the morphologies and biosensing parameters of the nano-/microstructured NiCo2O4 are also highlighted.Finally,some drawbacks and future perspectives related to this promising field are outlined.
基金the Open Fund of the Key Laboratory Dairy Science Project, Ministry of Education of China (KLDS2006-08B)
文摘As a new kind of analytical instrument,the principles,types and characters of biosensors were discussed in this paper.A biosensor is usually composed of a recognition element of biological origin and a physicochemical transducer.The biological element is capable of sensing the presence,activity or concentration of a chemical analyse in solution.The sensing takes place either as a binding event or a biocatalytical event.These interactions produce a measurable change in a solution property,in which the transducer is converted into a quantifiable electrical signal.The principles,types and applications of biosensors in environmental inspection,food production,clinical medicine and military defense were reviewed,and the trends in research were predicated.Furthermore,an attempt had been made to describe the future development directions and prospects.
文摘In the past two decades,the biological and medical fields have seen great advances in the development of biosensors and bioehips capable of characterizing and quantifying biomolecules.This lecture is meant to discuss the development and applications of advanced electroanalysis,biophotonics,nanotechnology,MEMS- based biosensors and biochips for biomedical diagnostics and physical performances of athlete.
