By the Soxhlet extraction, ethanol is used as extraction solvent of lees to determinate the total phenol content of Xiaoqu spirits lees. Folin-phenol method is handled to extract and to determine the reducing ability ...By the Soxhlet extraction, ethanol is used as extraction solvent of lees to determinate the total phenol content of Xiaoqu spirits lees. Folin-phenol method is handled to extract and to determine the reducing ability of extraction. The total phenolic content in the most suitable condition is determinated by the Folin-phenol method and the optimum extraction technological condition of total phenol is determinated by the experiment. The experimental results show that the optimization conditions of the determination methods of total phenolic content are determined by 3.5 mL for the Folin phenol reagent, 1:1 for the 10% Na2CO3 ratio and 30 min for the chromogenic reaction at room temperature. The optimum extraction technological condition of total phenol from lees is as following: solid-liquid ratio 1:12, ethanol concentration 100%, extraction temperature 80℃, extraction time 10 h.展开更多
目的解析粳高粱和糯高粱酿造的川法小曲白酒风味物质差异。方法对发酵过程酒醅理化指标和出酒率进行了检测分析,利用顶空固相微萃取气相色谱质谱联用法(headspace solid phase microextraction coupled with gas chromatography-mass sp...目的解析粳高粱和糯高粱酿造的川法小曲白酒风味物质差异。方法对发酵过程酒醅理化指标和出酒率进行了检测分析,利用顶空固相微萃取气相色谱质谱联用法(headspace solid phase microextraction coupled with gas chromatography-mass spectrometry,HS-SPME-GC-MS)、氢火焰气相色谱法(gas chromatography flame ionization detector,GC-FID)、偏最小二乘判别分析(partial least squares discriminant analysis,PLS-DA)结合感官评定分析了基酒挥发性风味物质。结果糯高粱原料出酒率极显著高于粳高粱(P<0.01);HS-SPME-GC-MS共检测出39种物质,主要为酯类和醇类物质;粳高粱发酵基酒中异丁醇、异戊醇和乙醛含量显著高于糯高粱发酵基酒(P<0.05,P<0.01),糯高粱发酵基酒中乙酸乙酯和乙酸含量显著高于粳高粱发酵基酒(P<0.05,P<0.01)。结论出酒率和主要风味物质的差异都表明糯高粱酿造的小曲酒优于粳高粱。展开更多
文摘By the Soxhlet extraction, ethanol is used as extraction solvent of lees to determinate the total phenol content of Xiaoqu spirits lees. Folin-phenol method is handled to extract and to determine the reducing ability of extraction. The total phenolic content in the most suitable condition is determinated by the Folin-phenol method and the optimum extraction technological condition of total phenol is determinated by the experiment. The experimental results show that the optimization conditions of the determination methods of total phenolic content are determined by 3.5 mL for the Folin phenol reagent, 1:1 for the 10% Na2CO3 ratio and 30 min for the chromogenic reaction at room temperature. The optimum extraction technological condition of total phenol from lees is as following: solid-liquid ratio 1:12, ethanol concentration 100%, extraction temperature 80℃, extraction time 10 h.
