Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly...Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.展开更多
Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocreba...Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.展开更多
Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性...Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性组织。Williams 是伦敦一家布店店员,后来成为店主。起初该组织是一个俱乐部,拥有12名青年成员,他们提出的口号是:“提高布店和其他行业青年的灵性修养”。这个俱乐部迅速推广到英国各地。1850年传到澳大利亚,1851年传到北美洲,其后在北美洲各地得到空前发展。展开更多
随着信息技术和网络技术的发展,人们对时间同步的精度要求越来越高。网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。介绍了NTP协议的基本原理,Y.1731单端DM原理。通过NTP协议在Y.1731报文中的应用研究和实践,...随着信息技术和网络技术的发展,人们对时间同步的精度要求越来越高。网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。介绍了NTP协议的基本原理,Y.1731单端DM原理。通过NTP协议在Y.1731报文中的应用研究和实践,使传统的网络时间同步精度能达到毫秒级别。实验数据表明,文中所设计的网络时钟同步系统比传统的时钟同步具有更高的时钟同步精度。展开更多
We carried out a general survey of current situations of P.intermedius S.Y.Chen.Results show that P.intermedius S.Y.Chen is distributed in Dongkeng Township,Jingnan Township and Yanxi Township.It covers an area of 262...We carried out a general survey of current situations of P.intermedius S.Y.Chen.Results show that P.intermedius S.Y.Chen is distributed in Dongkeng Township,Jingnan Township and Yanxi Township.It covers an area of 262.51 hm2.The distribution areas are narrow and limited.What's worse,it suffers from serious destruction and has been withered in large areas.Besides,according to current introduction test,it is difficult to survive in other places.In accordance with Exploring the Criteria for Endangered Categories of Bamboo Species from China put forward by Ma Naixun et al from the Research Institute of Subtropical Forestry of Chinese Academy of Forestry(RISFCAF),we propose incorporating P.intermedius S.Y.Chen into vulnerable species for protection.展开更多
Llex cornuta var.fortunei S.Y.Hu is a good ornamental tree species for viewing fruits and leaves,and has a wide range of garden uses.Nutrition bowl cutting seedlings can improve the survival rate of L.cornut a var.for...Llex cornuta var.fortunei S.Y.Hu is a good ornamental tree species for viewing fruits and leaves,and has a wide range of garden uses.Nutrition bowl cutting seedlings can improve the survival rate of L.cornut a var.fortunei S.Y.Hu transplanting and greening,and has the advantages of no time limit for planting and saving management costs.Based on this,this paper introduced the container seedling double-covering cutting technology for L.cornut a var.fortunei,to provide a reference for the propagation of L.cornut a var.fortunei.展开更多
基金This work was funded by Chongqing Municipal Technology Innovation and Application Development Program(cstc2020jscx-gksb0001)Yunnan Academician(Expert)Workstation Project(202105AF150073).
文摘Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.
基金Natural Science Foundation of Hainan Province(No.820RC776)。
文摘Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.
文摘Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性组织。Williams 是伦敦一家布店店员,后来成为店主。起初该组织是一个俱乐部,拥有12名青年成员,他们提出的口号是:“提高布店和其他行业青年的灵性修养”。这个俱乐部迅速推广到英国各地。1850年传到澳大利亚,1851年传到北美洲,其后在北美洲各地得到空前发展。
文摘随着信息技术和网络技术的发展,人们对时间同步的精度要求越来越高。网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。介绍了NTP协议的基本原理,Y.1731单端DM原理。通过NTP协议在Y.1731报文中的应用研究和实践,使传统的网络时间同步精度能达到毫秒级别。实验数据表明,文中所设计的网络时钟同步系统比传统的时钟同步具有更高的时钟同步精度。
文摘We carried out a general survey of current situations of P.intermedius S.Y.Chen.Results show that P.intermedius S.Y.Chen is distributed in Dongkeng Township,Jingnan Township and Yanxi Township.It covers an area of 262.51 hm2.The distribution areas are narrow and limited.What's worse,it suffers from serious destruction and has been withered in large areas.Besides,according to current introduction test,it is difficult to survive in other places.In accordance with Exploring the Criteria for Endangered Categories of Bamboo Species from China put forward by Ma Naixun et al from the Research Institute of Subtropical Forestry of Chinese Academy of Forestry(RISFCAF),we propose incorporating P.intermedius S.Y.Chen into vulnerable species for protection.
文摘Llex cornuta var.fortunei S.Y.Hu is a good ornamental tree species for viewing fruits and leaves,and has a wide range of garden uses.Nutrition bowl cutting seedlings can improve the survival rate of L.cornut a var.fortunei S.Y.Hu transplanting and greening,and has the advantages of no time limit for planting and saving management costs.Based on this,this paper introduced the container seedling double-covering cutting technology for L.cornut a var.fortunei,to provide a reference for the propagation of L.cornut a var.fortunei.
