AIM: To study the influence of frontalis muscle flap suspension on ocular surface by analyzing the clinical features and inflammatory cytokines.METHODS: A prospective, observational case series. Thirty-one eyes of 25 ...AIM: To study the influence of frontalis muscle flap suspension on ocular surface by analyzing the clinical features and inflammatory cytokines.METHODS: A prospective, observational case series. Thirty-one eyes of 25 patients with severe congenital blepharoptosis who underwent frontalis muscle flap suspension surgery with at least 6 mo of follow-up were included in the study. The main outcome measures were margin reflex distance 1(MRD_1), degree of lagophthalmos, ocular surface disease index(OSDI), fluorescein staining(Fl), tear break-up time(BUT), Schirmer I test, and inflammatory cytokine assay.RESULTS: The degrees of lagophthalmos significantly increased after surgery. The OSDI scores significantly increased 1wk postoperatively and then decreased 4wk after operation. The Fl scores reflected corneal epithelial defects in sixteen patients at early stage postoperatively. The BUT and Schirmer I test values remained stable and did not show change compared to those before surgery. The inflammatory cytokines in conjunctival epithelial cells(including IL-1β, IL-6, IL-8, TNF-α, and IL-17A) significantly increased 1wk after the surgery(P<0.001), then returned to the normal level at 24wk postoperatively. The levels of inflammatory cytokine IL-1β, IL-6, IL-8, TNF-α, and IL-17A elevated significantly and were positively correlated with OSDI and Fl scores.CONCLUSION: Frontalis muscle flap suspension surgery results in lagophthalmos in early period of post-operation and relieved after months. The elevation of inflammatory cytokines level may participate in the occurrence of corneal epithelial defects at the early postoperative stage.展开更多
Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spindle ...Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spindle in muscle atrophy induced by simulated microgravity. Methods The immunohistochemical technique (ABC) and image analysis were used to assess the conjugated ubiquitin immunostaining and the cross sectional area of intrafusal and extrafusal fibers of soleus in simulated microgravity rats. Results ①Tail suspension caused a progressive loss of soleus mass. Mean fiber CSA of extrafusal fibers were (7±2)%, (21±4)% and (32±7)% smaller after 3 days, 7 days and 14 days suspension, respectively. While the CAS of intrafusal fibers (bag + chain fibers) were (14±3)% ( P < 0.05 ), (30±7)% ( P < 0.01 ) and (44±10)% ( P < 0.01 ) smaller after 3 days, 7 days and 14 days suspension. ② The immunoreactivity of conjugated ubiquitin both in extrafusal and intrafusal fibers increased after tail suspension. The immunoreactivity of intrafusal fibers increased 1 day after suspension and reached the hightest level at 3 days after tail suspension. The immunoreactivity of extrafusal fibers increased after 3 days suspension and reached the highest level after 7 days tail suspension, which was lower than that in intrafusal fibers after 3 days tail suspension. Conclusion These results suggest that soleus atrophy of intrafusal fibers caused by tail suspension is earlier and more severe than that in extrafusal fibers.展开更多
目的:探讨生物反馈电刺激联合悬吊训练对产后盆底松弛综合征患者盆底肌力、阴道收缩功能及血清松弛素H2水平的影响。方法:选取2020年2月至2022年6月期间我科收治的83例产后盆底松弛综合征患者作为研究对象,按便利抽样法分为悬吊组(n=41...目的:探讨生物反馈电刺激联合悬吊训练对产后盆底松弛综合征患者盆底肌力、阴道收缩功能及血清松弛素H2水平的影响。方法:选取2020年2月至2022年6月期间我科收治的83例产后盆底松弛综合征患者作为研究对象,按便利抽样法分为悬吊组(n=41)和电刺激组(n=42)。悬吊组采用悬吊训练,电刺激组在悬吊组基础上增加生物反馈电刺激,对比两组患者盆底肌力、阴道收缩功能以及激素水平。结果:干预4 w后,电刺激组Ⅰ类、Ⅱ类肌纤维肌力、阴道收缩肌电值、动态压力、收缩持续时间、雌二醇(Estradiol,E2)水平、盆腔器官脱垂-尿失禁性生活量表(Pelvic organ prolapse-urinary incontinence sex scale,PISQ-12)评分明显高于悬吊组(P<0.05);电刺激组血清松弛素H2、松弛素受体(Relaxin receptor,LGR7)mRNA水平明显低于悬吊组。结论:在产后盆底松弛综合征患者中,联合应用生物反馈电刺激联合悬吊训练治疗有助于调节患者激素水平,提升盆底肌肉肌力,增强阴道收缩功能,提高性生活质量。展开更多
基金Supported by the National Natural Science Foundation of China(No.81670823)
文摘AIM: To study the influence of frontalis muscle flap suspension on ocular surface by analyzing the clinical features and inflammatory cytokines.METHODS: A prospective, observational case series. Thirty-one eyes of 25 patients with severe congenital blepharoptosis who underwent frontalis muscle flap suspension surgery with at least 6 mo of follow-up were included in the study. The main outcome measures were margin reflex distance 1(MRD_1), degree of lagophthalmos, ocular surface disease index(OSDI), fluorescein staining(Fl), tear break-up time(BUT), Schirmer I test, and inflammatory cytokine assay.RESULTS: The degrees of lagophthalmos significantly increased after surgery. The OSDI scores significantly increased 1wk postoperatively and then decreased 4wk after operation. The Fl scores reflected corneal epithelial defects in sixteen patients at early stage postoperatively. The BUT and Schirmer I test values remained stable and did not show change compared to those before surgery. The inflammatory cytokines in conjunctival epithelial cells(including IL-1β, IL-6, IL-8, TNF-α, and IL-17A) significantly increased 1wk after the surgery(P<0.001), then returned to the normal level at 24wk postoperatively. The levels of inflammatory cytokine IL-1β, IL-6, IL-8, TNF-α, and IL-17A elevated significantly and were positively correlated with OSDI and Fl scores.CONCLUSION: Frontalis muscle flap suspension surgery results in lagophthalmos in early period of post-operation and relieved after months. The elevation of inflammatory cytokines level may participate in the occurrence of corneal epithelial defects at the early postoperative stage.
文摘Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spindle in muscle atrophy induced by simulated microgravity. Methods The immunohistochemical technique (ABC) and image analysis were used to assess the conjugated ubiquitin immunostaining and the cross sectional area of intrafusal and extrafusal fibers of soleus in simulated microgravity rats. Results ①Tail suspension caused a progressive loss of soleus mass. Mean fiber CSA of extrafusal fibers were (7±2)%, (21±4)% and (32±7)% smaller after 3 days, 7 days and 14 days suspension, respectively. While the CAS of intrafusal fibers (bag + chain fibers) were (14±3)% ( P < 0.05 ), (30±7)% ( P < 0.01 ) and (44±10)% ( P < 0.01 ) smaller after 3 days, 7 days and 14 days suspension. ② The immunoreactivity of conjugated ubiquitin both in extrafusal and intrafusal fibers increased after tail suspension. The immunoreactivity of intrafusal fibers increased 1 day after suspension and reached the hightest level at 3 days after tail suspension. The immunoreactivity of extrafusal fibers increased after 3 days suspension and reached the highest level after 7 days tail suspension, which was lower than that in intrafusal fibers after 3 days tail suspension. Conclusion These results suggest that soleus atrophy of intrafusal fibers caused by tail suspension is earlier and more severe than that in extrafusal fibers.
文摘目的:探讨生物反馈电刺激联合悬吊训练对产后盆底松弛综合征患者盆底肌力、阴道收缩功能及血清松弛素H2水平的影响。方法:选取2020年2月至2022年6月期间我科收治的83例产后盆底松弛综合征患者作为研究对象,按便利抽样法分为悬吊组(n=41)和电刺激组(n=42)。悬吊组采用悬吊训练,电刺激组在悬吊组基础上增加生物反馈电刺激,对比两组患者盆底肌力、阴道收缩功能以及激素水平。结果:干预4 w后,电刺激组Ⅰ类、Ⅱ类肌纤维肌力、阴道收缩肌电值、动态压力、收缩持续时间、雌二醇(Estradiol,E2)水平、盆腔器官脱垂-尿失禁性生活量表(Pelvic organ prolapse-urinary incontinence sex scale,PISQ-12)评分明显高于悬吊组(P<0.05);电刺激组血清松弛素H2、松弛素受体(Relaxin receptor,LGR7)mRNA水平明显低于悬吊组。结论:在产后盆底松弛综合征患者中,联合应用生物反馈电刺激联合悬吊训练治疗有助于调节患者激素水平,提升盆底肌肉肌力,增强阴道收缩功能,提高性生活质量。
