Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacter...Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacterial composition of the supragingival plaques from the above-mentioned groups.Methods: Participants completed a questionnaire and were clinically examined for dental caries using decayed, missing and filled teeth index according to the criteria laid down by the World Health Organization. Supragingival plaque samples were collected from 50 caries-free adults(CF) and 50 caries-active adults(CA). Standard procedures of culture and identification of aerobic and anaerobic bacteria were used. Data were analyzed using Chi-square test.Results: A total of 117 bacterial strains were isolated from supragingival plaques in CF group subjects, 76(64.96%) of them belonged to 9 aerobic genera, and 41(35.04%) to 9anaerobic genera(P < 0.05). While in the second group, 199 strains were isolated, 119(59.80%) of the strains belonged to 10 aerobic genera and 80(40.20%) to 10 anaerobic bacteria(P < 0.05). Streptococcus mutans, Enterococcus faecium, Aerococcus viridans,Actinomyces meyeri, Lactobacillus acidophilus and Eubacterium limosum showed a significantly higher prevalence in the CA group(P < 0.05). The findings revealed that CA group had a high sugar intake(80%). A significantly higher frequency of tooth brushing(P < 0.000) and a significantly less self-reported oral malodor(P < 0.000) and tooth pain(P < 0.000) were found in CF group, while there was no association of socioeconomic levels and intake of meal snacks with dental caries.Conclusions: This study confirms the association of some aciduric bacteria with caries formation, and a direct association of sugar intake and cultural level with dental caries.Furthermore, oral hygiene practices minimize the prevalence of tooth decay.展开更多
Background: Nowadays, though more attention is being paid to oral and dental hygiene all over the world, dental caries and periodontal diseases are increasing daily. We decided to study the effectiveness of natural to...Background: Nowadays, though more attention is being paid to oral and dental hygiene all over the world, dental caries and periodontal diseases are increasing daily. We decided to study the effectiveness of natural toothbrush in prevention of dental caries and plaque formation. Methods: This analytic, semi-experimental clinical trial included 390 individuals. Subjects were divided into 3 groups: using natural toothbrush, using artificial toothbrush and using both natural and artificial toothbrush. The plaque index and DMFT was assessed at the beginning of the study until 1 year. ANOVA, Kruskal Wallis, LSD, Paired t-test, chi square, Fisher exact and Mc-Nemar tests were used for data analysis. Results: The plaque index in all three groups showed a mean reduction of 0.71. The mean DMFT in the samples before intervention was 8.38 ± 4.14 and increased to 9.1 ± 4.56. At the end of the study, the lowest plaque index was observed in the group using both the natural and artificial methods. During one year follow-up, the plaque index decreased 1.16, 0.83, and 0.21 degrees in the first, second and third groups. Conclusion: The increase in DMFT in the group that used artificial toothbrush was more than other groups. Thus, use of natural toothbrush leads to a decrease in growth rate of DMFT. Therefore natural toothbrush can be used for the cleaning of teeth and prevention of dental caries.展开更多
Destruction of calcified tissue was caused by acids which are by product of carbohydrate metabolism of acidogenic bacteria consequent to dental caries. The purpose of this study was to assess the existence of acidogen...Destruction of calcified tissue was caused by acids which are by product of carbohydrate metabolism of acidogenic bacteria consequent to dental caries. The purpose of this study was to assess the existence of acidogens potentially causing the dental caries and comparatively evaluation of efficacy of different toothpastes. The dental plaques of fifty persons belong to three age groups (1 - 20, 21 - 40, 41 - 60 year and above) were examined to identify microorganisms by the culture method. Thirty nine bacteria were isolated by spread plate method on BSMY I minimal media. Thirteen out of thirty nine, acidogens colonized in the dental plaques. Seven potentially acidogens CD17, CD26, CD27, CD28, CD29, CD34 and CD35 were treated with five different toothpastes. Inhibition effect of Triclosan and Fluoride containing tooth pastes were found more efficient. The results of the present study revealed that bacteria that commonly cause dental caries colonized in dental plaques of children and alcoholic person. Therefore, dental plaques must be considered a specific reservoir of colonization and subsequent dental caries. To reduce the dental problem triclosan and fluoride containing product should be recommended.展开更多
To obtain the caries experience and,plaque accumulation severity and pit and fissure morphology in first permanent molars in 7-8 children in Wuhan,as a reasonable prediction of caries risk and preventive attention in ...To obtain the caries experience and,plaque accumulation severity and pit and fissure morphology in first permanent molars in 7-8 children in Wuhan,as a reasonable prediction of caries risk and preventive attention in the future,a convenient sample of five primary schools in the vicinity of the Wuhan University School and Hospital of Stomatology was drawn.Two calibrated examiners orally examined all present grade 2 children in the classroom,using standard caries plaque and tooth morphology criteria.Dental caries was scored at enamel(D 2) and dentine(D 3) for tooth and surface level.Independent variables were age,gender and school.Data analysis used analysis of variance and t-test.The sample comprised 1 043 7-and 8-year-olds.The prevalence of dental caries in permanent dentition was 8.7% and in primary dentition,68.7%.Mean Decayed,Missing,Filled Teeth/S(DMFT/S) scores were 0.11 and 0.14,respectively.Mean dmft/s scores were 2.8 and 5.0.The d-component constituted 75% of the d 3 mft index,while enamel carious lesions constituted 36% of the total number of carious lesions(d 2,3-component).Prevalence of medium and deep pits and fissures was 84.6%.Prevalence of medium and severe plaque accumulation was 67.4%.Prevalence of dental caries in the deciduous and permanent dentitions of 7-to 8-year-old children was high.Deep pits and fissures in high caries risk children should be sealed.展开更多
Lactobacilli have been consistently associated with dental caries for decades;however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit iden...Lactobacilli have been consistently associated with dental caries for decades;however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit identification of oral Lactobacillus species is possible, despite their taxonomic complexity. Here we describe a combined approach involving both cultivation and genetic methods to ascertain and characterize the diversity and abundance of the Lactobacillus population in the oral cavities of children with severe early childhood caries (S-ECC). Eighty 3- to 6-year-old children (40 S-ECC and 40 caries free) who were seeking dental care at the Pediatric Dental Clinic of Bellevue Hospital in New York City were invited to participate in this study. Clinical data on sociodemographic information and oral health behavior were obtained from the primary caregiver. The data included a detailed dental examination, children’s medical history, and a questionnaire survey. Combined non-stimulated saliva and supragingival plaque samples were collected from each child and cultivated on selective media for quantitative measures of lactobacilli levels. The procedure for Lactobacillus species screening will include the random selection of 50 colonies per plate, ex- traction of DNA from each colony, and genotyping by arbitrarily primed polymerase chain reaction (AP-PCR). Each unique Lactobacillus AP-PCR genotype will be selected for taxonomic assessment by 16S rRNA gene sequencing analysis. Lactobacillus species will be identified by comparing the 16S rRNA sequences with the Ribosomal Database and the Human Oral Microbiome Database. Meanwhile, the same set of clinical samples will be independently subjected to genomic DNA isolation, 16S rRNA amplification with Lactobacillus genus-specific primers, sequencing, and taxonomic identification, both at genus and species levels with a customized pipeline. The distribution and phylogenetic differences of these Lactobacillus species will be compared between children with or without S-ECC. One of the main objectives of this study is to establish a study protocol for the identification and characterization of lactobacilli in the oral cavity. Future caries risk assessments can include lactobacilli counts (quantitative) and the presence/absence of specific cario- genic genetic signatures of a Lactobacillus species (qualitative) associated with S-ECC.展开更多
目的从微生物角度探究重度低龄儿童龋(severe early childhood caries,S⁃ECC)患儿一次完成龋病治疗前、治疗后3个月内菌斑微生物群落构成和多样性的变化及治疗对于远期无龋状态维持的作用。方法S⁃ECC患儿在全身麻醉下一次完成龋病治疗,...目的从微生物角度探究重度低龄儿童龋(severe early childhood caries,S⁃ECC)患儿一次完成龋病治疗前、治疗后3个月内菌斑微生物群落构成和多样性的变化及治疗对于远期无龋状态维持的作用。方法S⁃ECC患儿在全身麻醉下一次完成龋病治疗,采集治疗前(C)、术后7 d(C⁃7D)、1个月(C⁃1M)、3个月(C⁃3M)无龋牙面集合菌斑,并纳入无龋儿童(CF)为对照组,分析治疗前后菌斑微生物群落短期内的动态改建过程。结果S⁃ECC组和CF组菌斑群落组成高度相似;组间α多样性指数差异无统计学意义(P>0.05);从相对丰度值分析,纤毛菌属、聚集杆菌属等在治疗后较术前下降(P<0.05),C⁃7D组血链球菌较C组上升并在3个月内逐渐下降;治疗前韦荣菌属、放线菌属、拟普雷沃菌属、二氧化碳嗜纤维菌属、变异链球菌在C组和CF组间存在显著差异(P<0.05),其中变异链球菌经治疗后的C⁃7D、C⁃1M组与CF组未存在显著差异,而C⁃3M组较CF组出现上升(P<0.01)。结论S⁃ECC患儿在接受治疗后菌群结构的迅速改变,并在治疗后1~3个月逐渐开始建立新的无龋状态下的平衡。核心微生物群的存在使得菌斑群落保持稳定,韦荣菌属、拟普雷沃菌属、变异链球菌等具备作为龋病菌斑微生物标记物潜能。展开更多
基金Supported by the National Committee of Research Program and Assessment(Cnepru-code I02020130110)
文摘Objective: To compare oral hygiene practices, education and social background, food intake and oral malodor of Algerian adults suffering from dental caries with normal controls, and to determine and compare the bacterial composition of the supragingival plaques from the above-mentioned groups.Methods: Participants completed a questionnaire and were clinically examined for dental caries using decayed, missing and filled teeth index according to the criteria laid down by the World Health Organization. Supragingival plaque samples were collected from 50 caries-free adults(CF) and 50 caries-active adults(CA). Standard procedures of culture and identification of aerobic and anaerobic bacteria were used. Data were analyzed using Chi-square test.Results: A total of 117 bacterial strains were isolated from supragingival plaques in CF group subjects, 76(64.96%) of them belonged to 9 aerobic genera, and 41(35.04%) to 9anaerobic genera(P < 0.05). While in the second group, 199 strains were isolated, 119(59.80%) of the strains belonged to 10 aerobic genera and 80(40.20%) to 10 anaerobic bacteria(P < 0.05). Streptococcus mutans, Enterococcus faecium, Aerococcus viridans,Actinomyces meyeri, Lactobacillus acidophilus and Eubacterium limosum showed a significantly higher prevalence in the CA group(P < 0.05). The findings revealed that CA group had a high sugar intake(80%). A significantly higher frequency of tooth brushing(P < 0.000) and a significantly less self-reported oral malodor(P < 0.000) and tooth pain(P < 0.000) were found in CF group, while there was no association of socioeconomic levels and intake of meal snacks with dental caries.Conclusions: This study confirms the association of some aciduric bacteria with caries formation, and a direct association of sugar intake and cultural level with dental caries.Furthermore, oral hygiene practices minimize the prevalence of tooth decay.
文摘Background: Nowadays, though more attention is being paid to oral and dental hygiene all over the world, dental caries and periodontal diseases are increasing daily. We decided to study the effectiveness of natural toothbrush in prevention of dental caries and plaque formation. Methods: This analytic, semi-experimental clinical trial included 390 individuals. Subjects were divided into 3 groups: using natural toothbrush, using artificial toothbrush and using both natural and artificial toothbrush. The plaque index and DMFT was assessed at the beginning of the study until 1 year. ANOVA, Kruskal Wallis, LSD, Paired t-test, chi square, Fisher exact and Mc-Nemar tests were used for data analysis. Results: The plaque index in all three groups showed a mean reduction of 0.71. The mean DMFT in the samples before intervention was 8.38 ± 4.14 and increased to 9.1 ± 4.56. At the end of the study, the lowest plaque index was observed in the group using both the natural and artificial methods. During one year follow-up, the plaque index decreased 1.16, 0.83, and 0.21 degrees in the first, second and third groups. Conclusion: The increase in DMFT in the group that used artificial toothbrush was more than other groups. Thus, use of natural toothbrush leads to a decrease in growth rate of DMFT. Therefore natural toothbrush can be used for the cleaning of teeth and prevention of dental caries.
文摘Destruction of calcified tissue was caused by acids which are by product of carbohydrate metabolism of acidogenic bacteria consequent to dental caries. The purpose of this study was to assess the existence of acidogens potentially causing the dental caries and comparatively evaluation of efficacy of different toothpastes. The dental plaques of fifty persons belong to three age groups (1 - 20, 21 - 40, 41 - 60 year and above) were examined to identify microorganisms by the culture method. Thirty nine bacteria were isolated by spread plate method on BSMY I minimal media. Thirteen out of thirty nine, acidogens colonized in the dental plaques. Seven potentially acidogens CD17, CD26, CD27, CD28, CD29, CD34 and CD35 were treated with five different toothpastes. Inhibition effect of Triclosan and Fluoride containing tooth pastes were found more efficient. The results of the present study revealed that bacteria that commonly cause dental caries colonized in dental plaques of children and alcoholic person. Therefore, dental plaques must be considered a specific reservoir of colonization and subsequent dental caries. To reduce the dental problem triclosan and fluoride containing product should be recommended.
文摘To obtain the caries experience and,plaque accumulation severity and pit and fissure morphology in first permanent molars in 7-8 children in Wuhan,as a reasonable prediction of caries risk and preventive attention in the future,a convenient sample of five primary schools in the vicinity of the Wuhan University School and Hospital of Stomatology was drawn.Two calibrated examiners orally examined all present grade 2 children in the classroom,using standard caries plaque and tooth morphology criteria.Dental caries was scored at enamel(D 2) and dentine(D 3) for tooth and surface level.Independent variables were age,gender and school.Data analysis used analysis of variance and t-test.The sample comprised 1 043 7-and 8-year-olds.The prevalence of dental caries in permanent dentition was 8.7% and in primary dentition,68.7%.Mean Decayed,Missing,Filled Teeth/S(DMFT/S) scores were 0.11 and 0.14,respectively.Mean dmft/s scores were 2.8 and 5.0.The d-component constituted 75% of the d 3 mft index,while enamel carious lesions constituted 36% of the total number of carious lesions(d 2,3-component).Prevalence of medium and deep pits and fissures was 84.6%.Prevalence of medium and severe plaque accumulation was 67.4%.Prevalence of dental caries in the deciduous and permanent dentitions of 7-to 8-year-old children was high.Deep pits and fissures in high caries risk children should be sealed.
基金supported by the National Institute of Dental and Craniofacial Research(R01DE019455).
文摘Lactobacilli have been consistently associated with dental caries for decades;however, knowledge of this group of bacteria in the etiology of the disease is limited to quantitative elucidation. Nowadays, explicit identification of oral Lactobacillus species is possible, despite their taxonomic complexity. Here we describe a combined approach involving both cultivation and genetic methods to ascertain and characterize the diversity and abundance of the Lactobacillus population in the oral cavities of children with severe early childhood caries (S-ECC). Eighty 3- to 6-year-old children (40 S-ECC and 40 caries free) who were seeking dental care at the Pediatric Dental Clinic of Bellevue Hospital in New York City were invited to participate in this study. Clinical data on sociodemographic information and oral health behavior were obtained from the primary caregiver. The data included a detailed dental examination, children’s medical history, and a questionnaire survey. Combined non-stimulated saliva and supragingival plaque samples were collected from each child and cultivated on selective media for quantitative measures of lactobacilli levels. The procedure for Lactobacillus species screening will include the random selection of 50 colonies per plate, ex- traction of DNA from each colony, and genotyping by arbitrarily primed polymerase chain reaction (AP-PCR). Each unique Lactobacillus AP-PCR genotype will be selected for taxonomic assessment by 16S rRNA gene sequencing analysis. Lactobacillus species will be identified by comparing the 16S rRNA sequences with the Ribosomal Database and the Human Oral Microbiome Database. Meanwhile, the same set of clinical samples will be independently subjected to genomic DNA isolation, 16S rRNA amplification with Lactobacillus genus-specific primers, sequencing, and taxonomic identification, both at genus and species levels with a customized pipeline. The distribution and phylogenetic differences of these Lactobacillus species will be compared between children with or without S-ECC. One of the main objectives of this study is to establish a study protocol for the identification and characterization of lactobacilli in the oral cavity. Future caries risk assessments can include lactobacilli counts (quantitative) and the presence/absence of specific cario- genic genetic signatures of a Lactobacillus species (qualitative) associated with S-ECC.
文摘目的从微生物角度探究重度低龄儿童龋(severe early childhood caries,S⁃ECC)患儿一次完成龋病治疗前、治疗后3个月内菌斑微生物群落构成和多样性的变化及治疗对于远期无龋状态维持的作用。方法S⁃ECC患儿在全身麻醉下一次完成龋病治疗,采集治疗前(C)、术后7 d(C⁃7D)、1个月(C⁃1M)、3个月(C⁃3M)无龋牙面集合菌斑,并纳入无龋儿童(CF)为对照组,分析治疗前后菌斑微生物群落短期内的动态改建过程。结果S⁃ECC组和CF组菌斑群落组成高度相似;组间α多样性指数差异无统计学意义(P>0.05);从相对丰度值分析,纤毛菌属、聚集杆菌属等在治疗后较术前下降(P<0.05),C⁃7D组血链球菌较C组上升并在3个月内逐渐下降;治疗前韦荣菌属、放线菌属、拟普雷沃菌属、二氧化碳嗜纤维菌属、变异链球菌在C组和CF组间存在显著差异(P<0.05),其中变异链球菌经治疗后的C⁃7D、C⁃1M组与CF组未存在显著差异,而C⁃3M组较CF组出现上升(P<0.01)。结论S⁃ECC患儿在接受治疗后菌群结构的迅速改变,并在治疗后1~3个月逐渐开始建立新的无龋状态下的平衡。核心微生物群的存在使得菌斑群落保持稳定,韦荣菌属、拟普雷沃菌属、变异链球菌等具备作为龋病菌斑微生物标记物潜能。
