AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been i...AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-andthrough defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.RESULTS: There were no significant differences between control and SHED-seeded groups in connective tissue(P = 0.248), woven bone(P = 0.248) and compact bone(P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.CONCLUSION: SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.展开更多
BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teet...BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teeth(SHED),which are easy to obtain in a non-invasive manner,show pronounced proliferative and immunomodulatory capacities.AIM To investigate the protective effects of SHED on concanavalin A(ConA)-induced hepatitis in mice,and to elucidate the associated regulatory mechanisms.METHODS We used a ConA-induced acute hepatitis mouse model and an in vitro co-culture system to study the protective effects of SHED on ConA-induced autoimmune hepatitis,as well as the associated underlying mechanisms.RESULTS SHED infusion could prevent aberrant histopathological liver architecture caused by ConA-induced infiltration of CD3+,CD4+,tumor necrosis-alpha+,and interferon-gamma+inflammatory cells.Alanine aminotransferase and aspartate aminotransferase were significantly elevated in hepatitis mice.SHED infusion could therefore block ConA-induced alanine aminotransferase and aspartate aminotransferase elevations.Mechanistically,ConA upregulated tumor necrosisalpha and interferon-gamma expression,which was activated by the nuclear factor-kappa B pathway to induce hepatocyte apoptosis,resulting in acute liver injury.SHED administration protected hepatocytes from ConA-induced apoptosis.CONCLUSION SHED alleviates ConA-induced acute liver injury via inhibition of hepatocyte apoptosis mediated by the nuclear factor-kappa B pathway.Our findings could provide a potential treatment strategy for hepatitis.展开更多
The morphological characteristics of periodontal tissue and tooth tissue in gingival biotype are one of the indicators reflecting the individual differences of periodontal tissue in patients.Gingival biotypes of anter...The morphological characteristics of periodontal tissue and tooth tissue in gingival biotype are one of the indicators reflecting the individual differences of periodontal tissue in patients.Gingival biotypes of anterior teeth are often related to the prognosis of smile aesthetic treatment,which is one of the reference indexes for predicting the success rate of aesthetic treatment such as restoration,implant,periodontal,orthodontic and so on.Gingival biotypes have individual differences,so different gingival biotypes have different responses to different external stimuli.In the current clinical work,the correct evaluation of gingival biotype,especially the accurate measurement of gingival thickness,is the basis of reasonable choice of treatment and prognosis evaluation.展开更多
Objective:To explore the cure rate of bracketless invisible headgear orthodontic for opening deep occlusion of anterior teeth.Methods:10 cases with deep occlusion of anterior teeth were selected.All of the patients we...Objective:To explore the cure rate of bracketless invisible headgear orthodontic for opening deep occlusion of anterior teeth.Methods:10 cases with deep occlusion of anterior teeth were selected.All of the patients were admitted to our hospital from January 2017 to January 2020.All selected patients received bracketless invisible headgear orthodontic treatment.We took CBCT(cone beam CT)before and after orthodontics lateral cranial radiographs and curved tomographic radiographs to analyze the effect of orthodontic treatment in 10 patients.Results:The changes of 10 patients before and after the orthodontic treatment were not significant in Ul-SN,U6-SN,MP-FH(P>0.05);the Ul-PP of the patients all decreased by 3〜5mm.Compared with the effect before orthodontic treatment,the difference is significance(P<0.05);The tooth loosening didn't occur obviously in 10 patients.And 2 patients(20.00%)had mild root resorption in the anterior teeth.Conclusion:The effect of invisible headgear orthodontic treatment without brackets is significant in orthodontic treatment of patients with deep occlusion of anterior teeth.The pressure of the upper anterior teeth can reach as low as 3~5mm.The cure rate of deep occlusion of open anterior teeth is high.The orthodontic method is comfortable,with beautiful appearance,which is easier to be accepted by patients.展开更多
文摘AIM: To investigate the effect of stem cells from human exfoliated deciduous teeth(SHED) transplanted for bone regeneration in the dog mandibular defect.METHODS: In this prospective comparative study, SHEDs had been isolated 5 years ago from human exfoliated deciduous teeth. The undifferentiated stem cells were seeded into mandibular bone through-andthrough defects of 4 dogs. Similar defects in control group were filled with cell-free collagen scaffold. After 12 wk, biopsies were taken and morphometric analysis was performed. The percentage of new bone formation and foreign body reaction were measured in each case. The data were subject to statistical analysis using the Mann-Whitney U and Kruskalwalis statistical tests. Differences at P < 0.05 was considered as significant level.RESULTS: There were no significant differences between control and SHED-seeded groups in connective tissue(P = 0.248), woven bone(P = 0.248) and compact bone(P = 0.082). There were not any side effects in transplanted SHED group such as teratoma or malignancy and abnormalities in this period.CONCLUSION: SHEDs which had been isolated and characterized 5 years ago and stored with cryopreservation banking were capable of proliferation and osteogenesis after 5 years, and no immune response was observed after three months of seeded SHEDs.
基金Supported by The National Natural Science Foundation of China,No.81970940 and No.81600865Beijing Natural Science Foundation,No.7182182and the National Science and Technology Major Project of the Ministry of Science and Technology of China,No.2018ZX10302207.
文摘BACKGROUND Autoimmune hepatitis is a serious autoimmune liver disease that threatens human health worldwide,which emphasizes the urgent need to identify novel treatments.Stem cells from human exfoliated deciduous teeth(SHED),which are easy to obtain in a non-invasive manner,show pronounced proliferative and immunomodulatory capacities.AIM To investigate the protective effects of SHED on concanavalin A(ConA)-induced hepatitis in mice,and to elucidate the associated regulatory mechanisms.METHODS We used a ConA-induced acute hepatitis mouse model and an in vitro co-culture system to study the protective effects of SHED on ConA-induced autoimmune hepatitis,as well as the associated underlying mechanisms.RESULTS SHED infusion could prevent aberrant histopathological liver architecture caused by ConA-induced infiltration of CD3+,CD4+,tumor necrosis-alpha+,and interferon-gamma+inflammatory cells.Alanine aminotransferase and aspartate aminotransferase were significantly elevated in hepatitis mice.SHED infusion could therefore block ConA-induced alanine aminotransferase and aspartate aminotransferase elevations.Mechanistically,ConA upregulated tumor necrosisalpha and interferon-gamma expression,which was activated by the nuclear factor-kappa B pathway to induce hepatocyte apoptosis,resulting in acute liver injury.SHED administration protected hepatocytes from ConA-induced apoptosis.CONCLUSION SHED alleviates ConA-induced acute liver injury via inhibition of hepatocyte apoptosis mediated by the nuclear factor-kappa B pathway.Our findings could provide a potential treatment strategy for hepatitis.
文摘The morphological characteristics of periodontal tissue and tooth tissue in gingival biotype are one of the indicators reflecting the individual differences of periodontal tissue in patients.Gingival biotypes of anterior teeth are often related to the prognosis of smile aesthetic treatment,which is one of the reference indexes for predicting the success rate of aesthetic treatment such as restoration,implant,periodontal,orthodontic and so on.Gingival biotypes have individual differences,so different gingival biotypes have different responses to different external stimuli.In the current clinical work,the correct evaluation of gingival biotype,especially the accurate measurement of gingival thickness,is the basis of reasonable choice of treatment and prognosis evaluation.
文摘Objective:To explore the cure rate of bracketless invisible headgear orthodontic for opening deep occlusion of anterior teeth.Methods:10 cases with deep occlusion of anterior teeth were selected.All of the patients were admitted to our hospital from January 2017 to January 2020.All selected patients received bracketless invisible headgear orthodontic treatment.We took CBCT(cone beam CT)before and after orthodontics lateral cranial radiographs and curved tomographic radiographs to analyze the effect of orthodontic treatment in 10 patients.Results:The changes of 10 patients before and after the orthodontic treatment were not significant in Ul-SN,U6-SN,MP-FH(P>0.05);the Ul-PP of the patients all decreased by 3〜5mm.Compared with the effect before orthodontic treatment,the difference is significance(P<0.05);The tooth loosening didn't occur obviously in 10 patients.And 2 patients(20.00%)had mild root resorption in the anterior teeth.Conclusion:The effect of invisible headgear orthodontic treatment without brackets is significant in orthodontic treatment of patients with deep occlusion of anterior teeth.The pressure of the upper anterior teeth can reach as low as 3~5mm.The cure rate of deep occlusion of open anterior teeth is high.The orthodontic method is comfortable,with beautiful appearance,which is easier to be accepted by patients.
