目的:探讨寒热中药的成分与TRP家族中TRPV1和TRPM8通道蛋白mRNA表达的相关性。方法:原代培养DRG神经元,在体外观察中药单体对通道蛋白表达的影响,基因的表达量采用荧光定量PCR(real time PCR)法检测,数据分析采用2?ΔΔCT法。结果:热性...目的:探讨寒热中药的成分与TRP家族中TRPV1和TRPM8通道蛋白mRNA表达的相关性。方法:原代培养DRG神经元,在体外观察中药单体对通道蛋白表达的影响,基因的表达量采用荧光定量PCR(real time PCR)法检测,数据分析采用2?ΔΔCT法。结果:热性中药的成分(吴茱萸碱、桂皮醛)上调TRPV1的表达,下调TRPM8的表达,尤以寒负荷后更为明显;寒性中药的成分(黄芩苷、大黄素)上调TRPM8的表达,下调TRPV1的表达,尤以热负荷后更为明显。结论:对TRPV1与TR-PM8的表达调节可能与中药的寒热药性相关,这可能是寒热性中药临床上发挥寒热调节作用的机制之一。展开更多
To observe the effects of phenylallyl compounds on prostaglandin E2（PGE2）release in mouse cerebral microvascular endothelial cells （bEnd.3） stimulated by IL-1β, and to analyze their structure-activity relationshi...To observe the effects of phenylallyl compounds on prostaglandin E2（PGE2）release in mouse cerebral microvascular endothelial cells （bEnd.3） stimulated by IL-1β, and to analyze their structure-activity relationship. Different concentrations of phenylallyl compounds were added separately, and the content of PGE2 induced by IL-1β in the culture media was measured by ELISA assay. The 50% inhibitory concentration （IC 50 ） of PGE2 was calculated. Studies showed that phenylallyl compounds could affect the PGE2 release differently in bEnd.3 cells induced by IL-1β. Close relationships were shown between the inhibitory activities and the location and number of the substituent groups. In conclusion, phenylallyl compounds exhibited inhibitory activities at different extent on PGE2 release in bEnd.3 cells stimulated by IL-1β and presented certain structure-activity relationship.展开更多
文摘目的:探讨寒热中药的成分与TRP家族中TRPV1和TRPM8通道蛋白mRNA表达的相关性。方法:原代培养DRG神经元,在体外观察中药单体对通道蛋白表达的影响,基因的表达量采用荧光定量PCR(real time PCR)法检测,数据分析采用2?ΔΔCT法。结果:热性中药的成分(吴茱萸碱、桂皮醛)上调TRPV1的表达,下调TRPM8的表达,尤以寒负荷后更为明显;寒性中药的成分(黄芩苷、大黄素)上调TRPM8的表达,下调TRPV1的表达,尤以热负荷后更为明显。结论:对TRPV1与TR-PM8的表达调节可能与中药的寒热药性相关,这可能是寒热性中药临床上发挥寒热调节作用的机制之一。
文摘To observe the effects of phenylallyl compounds on prostaglandin E2（PGE2）release in mouse cerebral microvascular endothelial cells （bEnd.3） stimulated by IL-1β, and to analyze their structure-activity relationship. Different concentrations of phenylallyl compounds were added separately, and the content of PGE2 induced by IL-1β in the culture media was measured by ELISA assay. The 50% inhibitory concentration （IC 50 ） of PGE2 was calculated. Studies showed that phenylallyl compounds could affect the PGE2 release differently in bEnd.3 cells induced by IL-1β. Close relationships were shown between the inhibitory activities and the location and number of the substituent groups. In conclusion, phenylallyl compounds exhibited inhibitory activities at different extent on PGE2 release in bEnd.3 cells stimulated by IL-1β and presented certain structure-activity relationship.
