华支睾吸虫病(又称肝吸虫病)是一种常见的食源性寄生虫病,由华支睾吸虫感染导致,寄生于人体肝胆管系统,严重危害人体健康,为重要的人兽共患病。本文从病原学、免疫学、影像学及分子生物学四个领域对华支睾吸虫感染的诊断方法及研究进展...华支睾吸虫病(又称肝吸虫病)是一种常见的食源性寄生虫病,由华支睾吸虫感染导致,寄生于人体肝胆管系统,严重危害人体健康,为重要的人兽共患病。本文从病原学、免疫学、影像学及分子生物学四个领域对华支睾吸虫感染的诊断方法及研究进展进行归纳,为疾病的防控与诊断方法研究提供参考。Clonorchis sinensis (also known as liver flukes) is a common foodborne parasitic disease, caused by Clonorchis sinensis infection, parasitic in the human hepatobiliary system, seriously endangering human health, and is an important zoonotic disease. This article summarizes the diagnostic methods and research progress of Clonorchis sinensis infection from four fields: etiology, immunology, imaging and molecular biology, so as to provide reference for the research on disease prevention, control and diagnostic methods.展开更多
马尔尼菲篮状菌是双相型真菌,易感染免疫功能低下或缺陷的人群,临床症状包括突然发病、不规则且长时间高热、体质量减轻、呼吸系统症状、贫血和肝脾肿大等。本文报道1例艾滋病感染马尔尼菲篮状菌病的骨髓细胞学结果分析。Talaromyces ma...马尔尼菲篮状菌是双相型真菌,易感染免疫功能低下或缺陷的人群,临床症状包括突然发病、不规则且长时间高热、体质量减轻、呼吸系统症状、贫血和肝脾肿大等。本文报道1例艾滋病感染马尔尼菲篮状菌病的骨髓细胞学结果分析。Talaromyces marneffei is a biphasic fungus that is susceptible to immunocompromised or deficient people, and its clinical symptoms include sudden onset, irregular and prolonged high fever, weight loss, respiratory symptoms, anemia and hepatosplenomegaly. This article reports the analysis of bone marrow cytology results of 1 case of HIV-infected Talaromyces marneffei.展开更多
目的:探讨体检人群中Hcy水平及其与血脂水平的相关性。方法:分析106,406例体检人群Hcy水平,分别按性别及Hcy水平高低分组,分析Hcy与血脂水平(包括甘油三酯、总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇)的关系。结果:总体人群Hc...目的:探讨体检人群中Hcy水平及其与血脂水平的相关性。方法:分析106,406例体检人群Hcy水平,分别按性别及Hcy水平高低分组,分析Hcy与血脂水平(包括甘油三酯、总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇)的关系。结果:总体人群Hcy水平为13.06 μmol/L。男性组Hcy水平为13.98 μmol/L,女性组Hcy水平为11.14 μmol/L,女性组明显低于男性组(P Objective: To investigate the levels of Homocysteine (Hcy) and their correlation with lipid levels in a population undergoing health checkups. Methods: The study analyzed the Hcy levels of 106,406 individuals and categorized them by gender and Hcy levels. The relationship between Hcy and lipids levels (including triglycerides, total cholesterol, Low-Density Lipoprotein Cholesterol (LDL-C), and High-Density Lipoprotein Cholesterol (HDL-C)) was assessed. Results: The average Hcy level in the overall population was 13.06 μmol/L. The Hcy level for males was 13.98 μmol/L, while for females, it was 11.14 μmol/L, which was significantly lower than that of males (P < 0.05). The low Hcy group had significantly lower levels of Total Cholesterol (TC) and LDL-C compared to the high Hcy group (P < 0.05), while the HDL-C level in the low Hcy group was significantly higher than in the high Hcy group (P < 0.05). Conclusion: There is a gender difference in Hcy levels. In the health checkup population, Hcy levels are positively correlated with LDL-C and TC, and negatively correlated with HDL-C. Monitoring Hcy levels in key populations should be strengthened to better understand the relationship between Hcy and lipid levels, which can provide references for the prevention and treatment of lipid metabolism disorders in clinical practice.展开更多
快速、灵敏和特异性地检测病原微生物,在临床诊断和传染病控制中具有重要意义。早期准确检测是快速控制疫区疫情的有效措施,尤其是在缺乏有效治疗和疫苗的情况下。聚合酶链式反应(PCR)作为常用的核酸检测技术和疾病诊断的“金标准”,有...快速、灵敏和特异性地检测病原微生物,在临床诊断和传染病控制中具有重要意义。早期准确检测是快速控制疫区疫情的有效措施,尤其是在缺乏有效治疗和疫苗的情况下。聚合酶链式反应(PCR)作为常用的核酸检测技术和疾病诊断的“金标准”,有着高灵敏度的优势,但同时也存在以牺牲特异性为代价的现象。酶联免疫吸附试验(ELISA)是一种快速、特异性强的蛋白质和小分子诊断工具。然而,灵敏度低和样品预处理复杂的操作步骤极大地限制了该方法现场检测的应用。因此,快速、灵敏和特异性的检测技术成为了急需解决的要点,随着技术的应用和发展,基于CRISPR-Cas的生物传感系统的优异性能在开发病原微生物诊断技术方面引起了人们的关注。本文综述了CRISPR/Cas系统在病原微生物检测的作用机制及原理,总结了新型检测技术的优缺点并对应用发展前景进行展望。Rapid, sensitive, and specific detection of pathogenic microorganisms is crucial for clinical diagnosis and infectious disease control. Early and accurate detection is an effective measure to quickly control epidemic outbreaks, especially in the absence of effective treatments and vaccines. Polymerase Chain Reaction (PCR) is a commonly used nucleic acid testing technology and the “gold standard” for disease diagnosis with high sensitivity. However, it often sacrifices specificity. Enzyme-Linked Immunosorbent Assay (ELISA) is a rapid and highly specific diagnostic tool for proteins and small molecules. Nevertheless, its low sensitivity and complex sample preprocessing steps greatly limit its application in on-site testing. Therefore, the development of rapid, sensitive, and specific detection technologies has become an urgent need. With the application and advancement of technology, the excellent performance of CRISPR-Cas based biosensing systems has attracted attention in the development of pathogenic microorganism diagnostic techniques. This article reviews the mechanisms and principles of CRISPR/Cas systems in pathogenic microorganism detection, summarizes the advantages and disadvantages of novel detection technologies, and provides an outlook on their future applications.展开更多
目的:回顾性分析本院患儿九项呼吸道病原体感染的分布情况,为临床诊疗提供依据。方法:选取2023年1月~2023年12月至我院门诊就诊或住院治疗的748例急性呼吸道感染患儿作为研究对象,采用间接免疫荧光法对所有患儿的血清标本进行九项呼吸...目的:回顾性分析本院患儿九项呼吸道病原体感染的分布情况,为临床诊疗提供依据。方法:选取2023年1月~2023年12月至我院门诊就诊或住院治疗的748例急性呼吸道感染患儿作为研究对象,采用间接免疫荧光法对所有患儿的血清标本进行九项呼吸道病原体检测及分析。结果:748例患儿血清标本中共检测出阳性例数为281例,总阳性率为37.57%。其中男性患儿的阳性率为19.25%,女性阳患儿的阳性率为18.32%,两者差异无统计学意义(P > 0.05)。在单一呼吸道病原体阳性的患儿中,主要以肺炎支原体最常见,占阳性例数的27.14%,其次为副流感病毒和呼吸道合胞病毒,分别为3.07%和0.94%;混合感染有44例,占感染比例的5.88%。结论:儿童呼吸道感染主要是以肺炎支原体为主,其次为副流感病毒和呼吸道合胞病毒,及时检测出患儿呼吸道感染病原体的类型,为临床对患儿的诊疗具有重要的指导意义。Objective: To retrospectively analyze the distribution of nine respiratory tract pathogen infection in children, so as to provide evidence for diagnosis and treatment. Methods: 748 children with respiratory tract infection admitted into from January 2023 to December 2023 were selected as study subjects. All serum specimens of all children were tested by nine joint inspections of respiratory pathogens and analyzed. Results: Among the 748 patients, 281 were positive, with a positive rate of 37.57%, which that 19.25% were male children and 18.32% were female children and there was no significant difference statistically (P > 0.05). Mycoplasma pneumoniae (MP) was the most common among the children who were positive for single respiratory pathogens, accounting for 27.14% of the positive cases, followed by parainfluenza virus (HPIV) and respiratory syncytial virus (RSV), with a positive rate of 3.07% and 0.94%, respectively. There were 44 cases of mixed infection, accounting for 5.88% in all the positive patients. Conclusion: MP is the main pathogen of respiratory tract infection in children, and followed by the HPIV and RSV, so timely detection of the type of respiratory tract infection pathogens in children has important guiding significance for clinical diagnosis and treatment.展开更多
目的:了解本院产科门诊孕期妇女乙型肝炎病毒感染情况,为围产期进行干预管理,预防母婴传播提供参考依据。方法:回顾性分析2022年8月至2023年12月在本院产科门诊接受初次孕产期保健的孕期妇女进行血清乙型肝炎五项检测的实验结果。结果:...目的:了解本院产科门诊孕期妇女乙型肝炎病毒感染情况,为围产期进行干预管理,预防母婴传播提供参考依据。方法:回顾性分析2022年8月至2023年12月在本院产科门诊接受初次孕产期保健的孕期妇女进行血清乙型肝炎五项检测的实验结果。结果:受检2359人中乙肝HBsAg阳性187人,阳性率7.93%,其中,>40岁组检出阳性11例(18.33%)感染率最高,≤25岁组检出阳性9例(4.13%)感染率最低,26~30岁组检出阳性51例(6.17%),31~35岁组检出阳性71例(8.39%),36~40岁组检出阳性45例(11.00%),通过对不同年龄组HBsAg阳性率比较差异有统计学意义(P 40岁组检出HBsAb阳性37例(61.67%),通过不同年龄组的比较,差异具有统计学意义(P Objective: To understand the infection status of hepatitis B virus in pregnant women in the obstetrics outpatient department of our hospital, and provide reference for intervention and management during the perinatal period to prevent mother to child transmission. Method: A retrospective analysis was conducted on the experimental results of five serum hepatitis B tests conducted on pregnant women who received their first prenatal care at the obstetrics clinic of our hospital from August 2022 to December 2023. Results: Among 2359 people tested, 187 were positive for hepatitis B HBsAg, with a positive rate of 7.93%. Among them, 11 cases (18.33%) were positive in the group over 40 years of age, and 9 cases (4.13%) were positive in the group under 25 years of age. The infection rate was the lowest, 51 cases (6.17%) were positive in the group between 26 and 30 years of age, 71 cases (8.39%) were positive in the group between 31 and 35 years of age, and 45 cases (11.00%) were positive in the group between 36 and 40 years of age. By comparing the positive rates of HBsAg in different age groups, there was a statistically significant difference (P 40 age group. The differences between different age groups were statistically significant (P < 0.05). Conclusion: HBsAg positive is the most important risk factor of neonatal hepatitis B infection. Early determination of pregnant women’s hepatitis B infection can reduce the risk of mother to child transmission of hepatitis B, and ensure maternal and infant health and social stability;A large number of pregnant women are still negative for HBsAb, so it should be recommended to vaccinate hepatitis B vaccine in time.展开更多
目的:统计分析桂林市2010~2019年腹泻儿童轮状病毒(Rotavirus, RV)感染特征。方法:收集我院2010~2019年收治在门诊、住院的5岁以下腹泻儿童大便样本,采用免疫胶体金方法对其进行RV抗原检测。结果:腹泻儿童A群轮状病毒(Rotavirus A, RV-A...目的:统计分析桂林市2010~2019年腹泻儿童轮状病毒(Rotavirus, RV)感染特征。方法:收集我院2010~2019年收治在门诊、住院的5岁以下腹泻儿童大便样本,采用免疫胶体金方法对其进行RV抗原检测。结果:腹泻儿童A群轮状病毒(Rotavirus A, RV-A)总感染率为20.94%,年检出率分别为36.13%、35.35%、24.82%、22.41%、13.85%、10.15%、18.63%、22.91%、21.80%、16.95%;0~【6M、6M~【1Y、1Y~【2Y、2Y~【3Y、3~【4Y、4~≤ 5Y患儿RV-A感染率分别为14.95%、28.09%、39.17%、29.26%、21.24%、13.94%,其中1~【2Y组的检出率最高为(39.17%),与其他组比较分析其差异具有统计学意义(P 【0.05);12、1、2月的检出率分别为30.01%、30.10%、26.13%,与其他月份比较,差异具有统计学意义(P 【0.05)。结论:RV-A是桂林市5岁以下儿童腹泻的重要病原体,其感染与年龄、性别和季节有关。病原体感染高峰期发生在秋、冬季节,如11、12、1、2月,6M到3Y儿童为RV-A感染的高危人群,应做好预防工作。展开更多
文摘华支睾吸虫病(又称肝吸虫病)是一种常见的食源性寄生虫病,由华支睾吸虫感染导致,寄生于人体肝胆管系统,严重危害人体健康,为重要的人兽共患病。本文从病原学、免疫学、影像学及分子生物学四个领域对华支睾吸虫感染的诊断方法及研究进展进行归纳,为疾病的防控与诊断方法研究提供参考。Clonorchis sinensis (also known as liver flukes) is a common foodborne parasitic disease, caused by Clonorchis sinensis infection, parasitic in the human hepatobiliary system, seriously endangering human health, and is an important zoonotic disease. This article summarizes the diagnostic methods and research progress of Clonorchis sinensis infection from four fields: etiology, immunology, imaging and molecular biology, so as to provide reference for the research on disease prevention, control and diagnostic methods.
文摘马尔尼菲篮状菌是双相型真菌,易感染免疫功能低下或缺陷的人群,临床症状包括突然发病、不规则且长时间高热、体质量减轻、呼吸系统症状、贫血和肝脾肿大等。本文报道1例艾滋病感染马尔尼菲篮状菌病的骨髓细胞学结果分析。Talaromyces marneffei is a biphasic fungus that is susceptible to immunocompromised or deficient people, and its clinical symptoms include sudden onset, irregular and prolonged high fever, weight loss, respiratory symptoms, anemia and hepatosplenomegaly. This article reports the analysis of bone marrow cytology results of 1 case of HIV-infected Talaromyces marneffei.
文摘目的:探讨体检人群中Hcy水平及其与血脂水平的相关性。方法:分析106,406例体检人群Hcy水平,分别按性别及Hcy水平高低分组,分析Hcy与血脂水平(包括甘油三酯、总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇)的关系。结果:总体人群Hcy水平为13.06 μmol/L。男性组Hcy水平为13.98 μmol/L,女性组Hcy水平为11.14 μmol/L,女性组明显低于男性组(P Objective: To investigate the levels of Homocysteine (Hcy) and their correlation with lipid levels in a population undergoing health checkups. Methods: The study analyzed the Hcy levels of 106,406 individuals and categorized them by gender and Hcy levels. The relationship between Hcy and lipids levels (including triglycerides, total cholesterol, Low-Density Lipoprotein Cholesterol (LDL-C), and High-Density Lipoprotein Cholesterol (HDL-C)) was assessed. Results: The average Hcy level in the overall population was 13.06 μmol/L. The Hcy level for males was 13.98 μmol/L, while for females, it was 11.14 μmol/L, which was significantly lower than that of males (P < 0.05). The low Hcy group had significantly lower levels of Total Cholesterol (TC) and LDL-C compared to the high Hcy group (P < 0.05), while the HDL-C level in the low Hcy group was significantly higher than in the high Hcy group (P < 0.05). Conclusion: There is a gender difference in Hcy levels. In the health checkup population, Hcy levels are positively correlated with LDL-C and TC, and negatively correlated with HDL-C. Monitoring Hcy levels in key populations should be strengthened to better understand the relationship between Hcy and lipid levels, which can provide references for the prevention and treatment of lipid metabolism disorders in clinical practice.
文摘快速、灵敏和特异性地检测病原微生物,在临床诊断和传染病控制中具有重要意义。早期准确检测是快速控制疫区疫情的有效措施,尤其是在缺乏有效治疗和疫苗的情况下。聚合酶链式反应(PCR)作为常用的核酸检测技术和疾病诊断的“金标准”,有着高灵敏度的优势,但同时也存在以牺牲特异性为代价的现象。酶联免疫吸附试验(ELISA)是一种快速、特异性强的蛋白质和小分子诊断工具。然而,灵敏度低和样品预处理复杂的操作步骤极大地限制了该方法现场检测的应用。因此,快速、灵敏和特异性的检测技术成为了急需解决的要点,随着技术的应用和发展,基于CRISPR-Cas的生物传感系统的优异性能在开发病原微生物诊断技术方面引起了人们的关注。本文综述了CRISPR/Cas系统在病原微生物检测的作用机制及原理,总结了新型检测技术的优缺点并对应用发展前景进行展望。Rapid, sensitive, and specific detection of pathogenic microorganisms is crucial for clinical diagnosis and infectious disease control. Early and accurate detection is an effective measure to quickly control epidemic outbreaks, especially in the absence of effective treatments and vaccines. Polymerase Chain Reaction (PCR) is a commonly used nucleic acid testing technology and the “gold standard” for disease diagnosis with high sensitivity. However, it often sacrifices specificity. Enzyme-Linked Immunosorbent Assay (ELISA) is a rapid and highly specific diagnostic tool for proteins and small molecules. Nevertheless, its low sensitivity and complex sample preprocessing steps greatly limit its application in on-site testing. Therefore, the development of rapid, sensitive, and specific detection technologies has become an urgent need. With the application and advancement of technology, the excellent performance of CRISPR-Cas based biosensing systems has attracted attention in the development of pathogenic microorganism diagnostic techniques. This article reviews the mechanisms and principles of CRISPR/Cas systems in pathogenic microorganism detection, summarizes the advantages and disadvantages of novel detection technologies, and provides an outlook on their future applications.
文摘目的:回顾性分析本院患儿九项呼吸道病原体感染的分布情况,为临床诊疗提供依据。方法:选取2023年1月~2023年12月至我院门诊就诊或住院治疗的748例急性呼吸道感染患儿作为研究对象,采用间接免疫荧光法对所有患儿的血清标本进行九项呼吸道病原体检测及分析。结果:748例患儿血清标本中共检测出阳性例数为281例,总阳性率为37.57%。其中男性患儿的阳性率为19.25%,女性阳患儿的阳性率为18.32%,两者差异无统计学意义(P > 0.05)。在单一呼吸道病原体阳性的患儿中,主要以肺炎支原体最常见,占阳性例数的27.14%,其次为副流感病毒和呼吸道合胞病毒,分别为3.07%和0.94%;混合感染有44例,占感染比例的5.88%。结论:儿童呼吸道感染主要是以肺炎支原体为主,其次为副流感病毒和呼吸道合胞病毒,及时检测出患儿呼吸道感染病原体的类型,为临床对患儿的诊疗具有重要的指导意义。Objective: To retrospectively analyze the distribution of nine respiratory tract pathogen infection in children, so as to provide evidence for diagnosis and treatment. Methods: 748 children with respiratory tract infection admitted into from January 2023 to December 2023 were selected as study subjects. All serum specimens of all children were tested by nine joint inspections of respiratory pathogens and analyzed. Results: Among the 748 patients, 281 were positive, with a positive rate of 37.57%, which that 19.25% were male children and 18.32% were female children and there was no significant difference statistically (P > 0.05). Mycoplasma pneumoniae (MP) was the most common among the children who were positive for single respiratory pathogens, accounting for 27.14% of the positive cases, followed by parainfluenza virus (HPIV) and respiratory syncytial virus (RSV), with a positive rate of 3.07% and 0.94%, respectively. There were 44 cases of mixed infection, accounting for 5.88% in all the positive patients. Conclusion: MP is the main pathogen of respiratory tract infection in children, and followed by the HPIV and RSV, so timely detection of the type of respiratory tract infection pathogens in children has important guiding significance for clinical diagnosis and treatment.
文摘目的:了解本院产科门诊孕期妇女乙型肝炎病毒感染情况,为围产期进行干预管理,预防母婴传播提供参考依据。方法:回顾性分析2022年8月至2023年12月在本院产科门诊接受初次孕产期保健的孕期妇女进行血清乙型肝炎五项检测的实验结果。结果:受检2359人中乙肝HBsAg阳性187人,阳性率7.93%,其中,>40岁组检出阳性11例(18.33%)感染率最高,≤25岁组检出阳性9例(4.13%)感染率最低,26~30岁组检出阳性51例(6.17%),31~35岁组检出阳性71例(8.39%),36~40岁组检出阳性45例(11.00%),通过对不同年龄组HBsAg阳性率比较差异有统计学意义(P 40岁组检出HBsAb阳性37例(61.67%),通过不同年龄组的比较,差异具有统计学意义(P Objective: To understand the infection status of hepatitis B virus in pregnant women in the obstetrics outpatient department of our hospital, and provide reference for intervention and management during the perinatal period to prevent mother to child transmission. Method: A retrospective analysis was conducted on the experimental results of five serum hepatitis B tests conducted on pregnant women who received their first prenatal care at the obstetrics clinic of our hospital from August 2022 to December 2023. Results: Among 2359 people tested, 187 were positive for hepatitis B HBsAg, with a positive rate of 7.93%. Among them, 11 cases (18.33%) were positive in the group over 40 years of age, and 9 cases (4.13%) were positive in the group under 25 years of age. The infection rate was the lowest, 51 cases (6.17%) were positive in the group between 26 and 30 years of age, 71 cases (8.39%) were positive in the group between 31 and 35 years of age, and 45 cases (11.00%) were positive in the group between 36 and 40 years of age. By comparing the positive rates of HBsAg in different age groups, there was a statistically significant difference (P 40 age group. The differences between different age groups were statistically significant (P < 0.05). Conclusion: HBsAg positive is the most important risk factor of neonatal hepatitis B infection. Early determination of pregnant women’s hepatitis B infection can reduce the risk of mother to child transmission of hepatitis B, and ensure maternal and infant health and social stability;A large number of pregnant women are still negative for HBsAb, so it should be recommended to vaccinate hepatitis B vaccine in time.
