The inclusion complexes of β-cyclodextrin(β-CD) with vanillin, sebacic acid and cholesterol were prepared. DSC analysis showed that the optimum qualitative ratio of three inclusion complexes was 1∶1, 2∶1, 3∶1, re...The inclusion complexes of β-cyclodextrin(β-CD) with vanillin, sebacic acid and cholesterol were prepared. DSC analysis showed that the optimum qualitative ratio of three inclusion complexes was 1∶1, 2∶1, 3∶1, respectively. XRD results of β-CD and its inclusion complexes indicate that the crystals of three inclusion complexes belong to an obvious channel-type packing structure, and the axial structure period is two times of the height of β-CD torus. The structural models of three inclusion complexes were proposed according to "head-head" alignment of β-CDs, molecular size match and polarity. A specific peak in XRD spectra indication of forming inclusion complex can be identified at 1 2 nm(about 7 degrees).展开更多
In this paper, we have studied the interaction of CuZnSODⅢand Outer Copper, enzyme activity experiments expressed that 0.1mmol·L-1 Cu2+addition reduced the enzyme activity sharply, but this reduced action had no...In this paper, we have studied the interaction of CuZnSODⅢand Outer Copper, enzyme activity experiments expressed that 0.1mmol·L-1 Cu2+addition reduced the enzyme activity sharply, but this reduced action had not been found for the additions of 0.1mmol·L-1 1∶1 Cu2+and Zn2+, and 0.1mmol·L-1 Zn2+, respectively. This was due to the Cu2+exchanged the Zn2+in CuZnSODⅢ,and it was proved by the experiment of determination of metal content. Meanwhile, the static fluorescence quenching mechanism revealed the exist of molecular complex of CuZnSOD with Cu2+. The binding constant was obtained from lineweaver burk and double lg plot. The distance of active site to Trp is about 2.83nm, was calculated according to Frster theory.展开更多
The stability constants of inclusion complexes of some benzene derivations with β cyclodextrin (β CD) in aqueous solution were studied by ultraviolet spectrophotometry at 20℃,30℃,40℃ and 50℃,respectively.The app...The stability constants of inclusion complexes of some benzene derivations with β cyclodextrin (β CD) in aqueous solution were studied by ultraviolet spectrophotometry at 20℃,30℃,40℃ and 50℃,respectively.The apparent thermodynamic parameters were also obtained.The results demonstrated that van der Waals force (Δ H <0,Δ S <0,governed by Δ H ) is the major driving force of inclusion systems of benzene derivations with β CD.On the other hand,in the case of inclusion complex of vanillin,the hydrogen bonding may also play a role in complexation.展开更多
文摘The inclusion complexes of β-cyclodextrin(β-CD) with vanillin, sebacic acid and cholesterol were prepared. DSC analysis showed that the optimum qualitative ratio of three inclusion complexes was 1∶1, 2∶1, 3∶1, respectively. XRD results of β-CD and its inclusion complexes indicate that the crystals of three inclusion complexes belong to an obvious channel-type packing structure, and the axial structure period is two times of the height of β-CD torus. The structural models of three inclusion complexes were proposed according to "head-head" alignment of β-CDs, molecular size match and polarity. A specific peak in XRD spectra indication of forming inclusion complex can be identified at 1 2 nm(about 7 degrees).
文摘In this paper, we have studied the interaction of CuZnSODⅢand Outer Copper, enzyme activity experiments expressed that 0.1mmol·L-1 Cu2+addition reduced the enzyme activity sharply, but this reduced action had not been found for the additions of 0.1mmol·L-1 1∶1 Cu2+and Zn2+, and 0.1mmol·L-1 Zn2+, respectively. This was due to the Cu2+exchanged the Zn2+in CuZnSODⅢ,and it was proved by the experiment of determination of metal content. Meanwhile, the static fluorescence quenching mechanism revealed the exist of molecular complex of CuZnSOD with Cu2+. The binding constant was obtained from lineweaver burk and double lg plot. The distance of active site to Trp is about 2.83nm, was calculated according to Frster theory.
文摘The stability constants of inclusion complexes of some benzene derivations with β cyclodextrin (β CD) in aqueous solution were studied by ultraviolet spectrophotometry at 20℃,30℃,40℃ and 50℃,respectively.The apparent thermodynamic parameters were also obtained.The results demonstrated that van der Waals force (Δ H <0,Δ S <0,governed by Δ H ) is the major driving force of inclusion systems of benzene derivations with β CD.On the other hand,in the case of inclusion complex of vanillin,the hydrogen bonding may also play a role in complexation.
