目的 :研究雄激素受体 (AR)基因在正常人外周血白细胞中的表达。方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。结果 :用 RT- PCR方法得到了 390 bp的 AR c DNA片段 ;用 Northern印迹方法检测出长...目的 :研究雄激素受体 (AR)基因在正常人外周血白细胞中的表达。方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。结果 :用 RT- PCR方法得到了 390 bp的 AR c DNA片段 ;用 Northern印迹方法检测出长约 9.4kb的 AR m RNA,与文献报道的人前列腺组织 AR m RNA大小相同。结论 :两种方法均证实了 AR在人外周血白细胞中的表达 ;该结果为研究雄激素影响白细胞功能的机制及探讨病理情况下展开更多
The expressed sequence tag of eukaryotic translation initiation factor 5 （eIF5） from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by ...The expressed sequence tag of eukaryotic translation initiation factor 5 （eIF5） from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame （GenBank accession number： AY686501）. The full-length cDNA of SjeIF5 was cloned into a pET-28c^（＋） vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coll. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.展开更多
文摘目的 :研究雄激素受体 (AR)基因在正常人外周血白细胞中的表达。方法 :采用 RT- PCR和 Northern印迹方法检测健康成年人外周血白细胞 AR m RNA的表达。结果 :用 RT- PCR方法得到了 390 bp的 AR c DNA片段 ;用 Northern印迹方法检测出长约 9.4kb的 AR m RNA,与文献报道的人前列腺组织 AR m RNA大小相同。结论 :两种方法均证实了 AR在人外周血白细胞中的表达 ;该结果为研究雄激素影响白细胞功能的机制及探讨病理情况下
文摘The expressed sequence tag of eukaryotic translation initiation factor 5 （eIF5） from the Schistosoma japonicum adult worm cDNA library through subtractive hybridization between male and female worms was analyzed by the bioinformatics method. The overlapping sequences were assembled into one that includes the complete open reading frame （GenBank accession number： AY686501）. The full-length cDNA of SjeIF5 was cloned into a pET-28c^（＋） vector, which generated a prokaryotic expression plasmid, and a fusion protein of 18 kDa was induced in Escherichia coll. The recombinant expression of eIF5 protein of Schistosoma japonicum was purified. The immunoprotection test against schistosomiasis demonstrated that the recombinant protein worked to a certain extent, especially in the reduction of eggs in the liver of the host.
基金supported by the Natural Science Foundation of China (30170224 ,30270310 and 30330180) ,the Chinese Academy of Sciences (KSCX-2-2-04) ,Committee of Science and Technologyin Shanghai (02DJ140567)
