从香蕉园或者其他果园的土壤中分离获得5株对香蕉枯萎病菌(Fusarium oxysporum f. sp.Cubense)具有抑制作用的拮抗菌,并对其抑菌效果和和机制进行了测定.结果表明,测试的5株拮抗菌d4、d5、d6、B3和p与香蕉枯萎病菌对峙培养5d后,在平板...从香蕉园或者其他果园的土壤中分离获得5株对香蕉枯萎病菌(Fusarium oxysporum f. sp.Cubense)具有抑制作用的拮抗菌,并对其抑菌效果和和机制进行了测定.结果表明,测试的5株拮抗菌d4、d5、d6、B3和p与香蕉枯萎病菌对峙培养5d后,在平板上产生明显的抑菌带,抑菌带宽度为16.5～20.5mm;培养10d后,仍然保持稳定的抑菌效果;5株拮抗菌发酵原液对孢子萌发具有显著的抑制作用,抑制率为90.49%～97.18%;拮抗菌对病菌的作用机制表现为对菌丝的消融、菌丝细胞的泡囊化、抑制病菌分生孢子的萌发、孢子芽管的扭曲.展开更多
Nimbya alternantherae is a leaf spot fungus isolated from Alternanthera philoxeroides.It could produce phytotoxin to Alternanthera philoxeroides in several kinds of liquid media,especially in modified Fries.The filtra...Nimbya alternantherae is a leaf spot fungus isolated from Alternanthera philoxeroides.It could produce phytotoxin to Alternanthera philoxeroides in several kinds of liquid media,especially in modified Fries.The filtrate of the pathogen was chromatographed on a DM-130 colophony column eluting with methanol,and evaporation of the solvent gave a brown mush.The crude product was then extracted with ethyl acetate.The toxin could be purified as white needles by re-crystallizing from benzene and acetone（volume ratio is 1∶1） and silica gel chromatograph.The results of elemental analysis,MS,IR and NMR indicate that molecular weight of the toxin was 240,formula was C11H12O6,and its chemical name was 2-acetyl-3,4-dihydroxy-5-methoxyphenyl-acetic acid（Vulculic acid）.展开更多
文摘从香蕉园或者其他果园的土壤中分离获得5株对香蕉枯萎病菌(Fusarium oxysporum f. sp.Cubense)具有抑制作用的拮抗菌,并对其抑菌效果和和机制进行了测定.结果表明,测试的5株拮抗菌d4、d5、d6、B3和p与香蕉枯萎病菌对峙培养5d后,在平板上产生明显的抑菌带,抑菌带宽度为16.5～20.5mm;培养10d后,仍然保持稳定的抑菌效果;5株拮抗菌发酵原液对孢子萌发具有显著的抑制作用,抑制率为90.49%～97.18%;拮抗菌对病菌的作用机制表现为对菌丝的消融、菌丝细胞的泡囊化、抑制病菌分生孢子的萌发、孢子芽管的扭曲.
文摘Nimbya alternantherae is a leaf spot fungus isolated from Alternanthera philoxeroides.It could produce phytotoxin to Alternanthera philoxeroides in several kinds of liquid media,especially in modified Fries.The filtrate of the pathogen was chromatographed on a DM-130 colophony column eluting with methanol,and evaporation of the solvent gave a brown mush.The crude product was then extracted with ethyl acetate.The toxin could be purified as white needles by re-crystallizing from benzene and acetone（volume ratio is 1∶1） and silica gel chromatograph.The results of elemental analysis,MS,IR and NMR indicate that molecular weight of the toxin was 240,formula was C11H12O6,and its chemical name was 2-acetyl-3,4-dihydroxy-5-methoxyphenyl-acetic acid（Vulculic acid）.
