间充质干细胞(MSCs)起源于胚胎的中胚层,具有在体内外分化为骨骼、软骨等多种组织的能力。这些干细胞在修复和再生医学中具有显著的应用潜力,尤其是在心脏、肝脏和皮肤损伤的修复。在骨科领域,MSCs能够促进骨折愈合,但其机制仍不完全明...间充质干细胞(MSCs)起源于胚胎的中胚层,具有在体内外分化为骨骼、软骨等多种组织的能力。这些干细胞在修复和再生医学中具有显著的应用潜力,尤其是在心脏、肝脏和皮肤损伤的修复。在骨科领域,MSCs能够促进骨折愈合,但其机制仍不完全明确。近年的研究表明,MSCs通过调节炎症反应、免疫系统和血管新生,以及促进成骨分化,对骨折愈合的各个阶段产生影响。通过分泌生长因子、外泌体和其他生物活性物质,这些细胞激活多条信号通路,加速骨折的愈合过程,并显示出在骨组织修复中的重要性。Mesenchymal stem cells (MSCs) originate from the mesoderm of the embryo and have the ability to differentiate into various tissues such as bone and cartilage in vitro and in vivo. These stem cells have significant potential for applications in restorative and regenerative medicine, especially in the repair of heart, liver, and skin damage. In orthopedics, MSCs can promote fracture healing, but the mechanism is still not fully understood. Recent studies have shown that MSCs have an impact on all stages of fracture healing by modulating the inflammatory response, immune system and angiogenesis, and promoting osteogenic differentiation. By secreting growth factors, exosomes, and other bioactive substances, these cells activate multiple signaling pathways, accelerate the healing process of fractures, and show importance in bone tissue repair.展开更多
目的观察长期稀土暴露者血清蛋白表达变化情况,在蛋白质水平探讨稀土元素致病理性纤维化的发病机制。方法选取稀土矿区工人及对照组各8人,分别剪取头发1g行稀土元素含量检测,抽静脉血4mL,利用同位素相对标记与绝对定量(isobaric tags fo...目的观察长期稀土暴露者血清蛋白表达变化情况,在蛋白质水平探讨稀土元素致病理性纤维化的发病机制。方法选取稀土矿区工人及对照组各8人,分别剪取头发1g行稀土元素含量检测,抽静脉血4mL,利用同位素相对标记与绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术行血清定量蛋白组学分析,筛选并鉴定差异蛋白,并对差异蛋白进行生物信息学分析。结果矿工组头发稀土含量明显高于对照组;蛋白组学结果显示矿工组29种蛋白表达异常,去除部分低可信度蛋白后剩余22种,其中与纤维化有关的粘多糖和纤维连接蛋白表达升高。结论粘多糖和纤维连接蛋白可能在稀土元素致病理性纤维化发生发展过程中发挥重要作用。展开更多
文摘间充质干细胞(MSCs)起源于胚胎的中胚层,具有在体内外分化为骨骼、软骨等多种组织的能力。这些干细胞在修复和再生医学中具有显著的应用潜力,尤其是在心脏、肝脏和皮肤损伤的修复。在骨科领域,MSCs能够促进骨折愈合,但其机制仍不完全明确。近年的研究表明,MSCs通过调节炎症反应、免疫系统和血管新生,以及促进成骨分化,对骨折愈合的各个阶段产生影响。通过分泌生长因子、外泌体和其他生物活性物质,这些细胞激活多条信号通路,加速骨折的愈合过程,并显示出在骨组织修复中的重要性。Mesenchymal stem cells (MSCs) originate from the mesoderm of the embryo and have the ability to differentiate into various tissues such as bone and cartilage in vitro and in vivo. These stem cells have significant potential for applications in restorative and regenerative medicine, especially in the repair of heart, liver, and skin damage. In orthopedics, MSCs can promote fracture healing, but the mechanism is still not fully understood. Recent studies have shown that MSCs have an impact on all stages of fracture healing by modulating the inflammatory response, immune system and angiogenesis, and promoting osteogenic differentiation. By secreting growth factors, exosomes, and other bioactive substances, these cells activate multiple signaling pathways, accelerate the healing process of fractures, and show importance in bone tissue repair.
文摘目的观察长期稀土暴露者血清蛋白表达变化情况,在蛋白质水平探讨稀土元素致病理性纤维化的发病机制。方法选取稀土矿区工人及对照组各8人,分别剪取头发1g行稀土元素含量检测,抽静脉血4mL,利用同位素相对标记与绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术行血清定量蛋白组学分析,筛选并鉴定差异蛋白,并对差异蛋白进行生物信息学分析。结果矿工组头发稀土含量明显高于对照组;蛋白组学结果显示矿工组29种蛋白表达异常,去除部分低可信度蛋白后剩余22种,其中与纤维化有关的粘多糖和纤维连接蛋白表达升高。结论粘多糖和纤维连接蛋白可能在稀土元素致病理性纤维化发生发展过程中发挥重要作用。
