食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的...食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的系统、全面综述。本文系统查阅了Web of Science、PubMed、SciFinder Academic和中国知网等数据库,对近20年有关食用菌多糖提取、分离、纯化和定性、定量分析方法的文献进行总结归纳,并对比分析各种方法的特点,旨在为食用菌多糖的深入研究提供参考。展开更多
Puerariae Radix （Ge-Gen in Chinese） is a popular traditional Chinese herbal medicine derived from the driedroots ofPueraria lobata （Willd.） Ohwi or P. thomsonii Benth. It shows significant pharmacological activiti...Puerariae Radix （Ge-Gen in Chinese） is a popular traditional Chinese herbal medicine derived from the driedroots ofPueraria lobata （Willd.） Ohwi or P. thomsonii Benth. It shows significant pharmacological activities in the treatment ofcardiovascular diseases, hypertension, and diabetes. This review summarizes progress in the chemistry study of Puerariaspecies, mainly covering the classes of flavonoids, puerosides, coumarins, triterpenoids, and triterpene saponins. Qualitative andquantitative chemical analyses are also covered.展开更多
The aim of this study was to isolate the chemical constituents of the aerial parts of Polygala tenuifolia Willd. and to determine their antioxidant activities. Ten flavonoids were isolated and purified by silica gel, ...The aim of this study was to isolate the chemical constituents of the aerial parts of Polygala tenuifolia Willd. and to determine their antioxidant activities. Ten flavonoids were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. Their structures were elucidated by spectroscopic analysis and identified as isorhamnetin-3-O-13-D-glucopyranoside （1）, isorhamnetin-3-O-13-D-galactopyranoside （2）, quercetin-3-O-13-o-glucopyranosyl （1--＊2）-I^-D-galactopyranoside （3）, quercetin-3-O-13-O-glucopyranosyl （1---~2）-13-D-glucopyranoside （4）, linarin （5）, quercetin-3-O- 13-D-glucopyranoside （6）, 5,7-dihydroxy-8-methxoyflavone-7-O-13-o-glucuronoside （7）, isorhamnetin （8）, kaempferol （9） and quercetin （10）. All these compounds were isolated from this plant for the first time, and compounds 1-5 and 7 were isolated from the genus of Polygala for the first time. The antioxidant activities of the isolated compounds were evaluated by DPPH free radical scavenging assay, and compounds 3, 4, 6, 8, 9 and 10 showed potent antioxidant activities.展开更多
A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of...A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.展开更多
Twenty-seven flavonoids, including 10 isoflavones, 9 flavanones, 3 dihydrochalcones, 3 flavonols and 2 flavans, were isolated from the whole plant of Tibetan medicine Oxytropis falcate Bunge. Structure elucidation was...Twenty-seven flavonoids, including 10 isoflavones, 9 flavanones, 3 dihydrochalcones, 3 flavonols and 2 flavans, were isolated from the whole plant of Tibetan medicine Oxytropis falcate Bunge. Structure elucidation was conducted by extensive spectroscopic analysis, including MS, NMR and CD spectra. Twelve compounds were obtained from this plant for the first time, among which 10 compounds were obtained from the genus of Oxytropis for the first time.展开更多
A reversed-phase high performance liquid chromatography （RP-HPLC） method with diode array detector （DAD） has been established to simultaneously determine six bioactive compounds in the roots ofllex pubescens, name...A reversed-phase high performance liquid chromatography （RP-HPLC） method with diode array detector （DAD） has been established to simultaneously determine six bioactive compounds in the roots ofllex pubescens, namely tortoside A, （＋）-syringaresinol, ilexsaponin B3, ilexsaponin A1, ilexsaponin B1, ilexgenin A. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phase consisted of acetonitrile and water containing 0.1% （v/v） phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm. The six marker constituents were separated well with good linearity （r2〉0.9996）, precision, stability and repeatability. The overall recoveries were in the range of 99.00%-104.52%. Cluster analysis was employed to analyze 15 batches of samples. The result indicated this method provide an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of I. pubescens.展开更多
A phytochemical investigation on the stems and rhizomes of Sinomenium acutum led to the isolation of 19 compounds, including three phenanthrenes, six anthraquinones, and ten phenolic derivatives. The structures of the...A phytochemical investigation on the stems and rhizomes of Sinomenium acutum led to the isolation of 19 compounds, including three phenanthrenes, six anthraquinones, and ten phenolic derivatives. The structures of the isolated compounds were elucidated by analysis of the MS and NMR spectroscopic data and comparison with the literature. Compounds 1 and 2 are two new natural products, and their 1H and 13C NMR spectral data were fully assigned for the first time, with the help of2D NMR. All of the isolates were obtained from genus Sinomenium for the first time and the phenanthrene and anthraquinone are the structure type first reported from this genus plants.展开更多
The aim of this study is to perform a phytochemical investigation of the aerial parts of Murraya tetramera Huang. Nine compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative...The aim of this study is to perform a phytochemical investigation of the aerial parts of Murraya tetramera Huang. Nine compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative TLC and HPLC methods. By analysis of the MS and NMR spectroscopic data and comparison with those in literature, these nine compounds were identified as 3,3＇-[oxybis（methylene）]bis（9-methoxy-9H-carbazole）（1）, murrastifoline B（2）, murrayaquinone A（3）, 2,4-dimethoxyphenol（4）, 1,2-dimethoxy-4-nitrobenzene（5）, 3-methylanisole（6）,（–）-syringaresinol-4-O-β-D-glucopyranoside（7）, β-sitosterol（8）, and octadecanyl-3-methoxy-4-hydroxylbenzeneacrylate（9）, respectively. Among them, compounds 4–6 and 9 were described from the genus Murraya for the first time, and compounds 2–9 were isolated from M. tetramera for the first time.展开更多
Ten compounds were isolated from Qianliang tea, a kind of dark tea fully fermented from the leaves of Camellia sinensis （L.） O. Kuntze vat. sinensis, and identified .as N-（2-hydroxyphenyl）-2-pyrrolidinone （1）, p...Ten compounds were isolated from Qianliang tea, a kind of dark tea fully fermented from the leaves of Camellia sinensis （L.） O. Kuntze vat. sinensis, and identified .as N-（2-hydroxyphenyl）-2-pyrrolidinone （1）, p-hydroxyacetophenone （2）, salicifoliol （3）, （-）-3-hydroxy-/q-ionone （4）, p-hydroxy ethyl ciunamate （5）, ethyl 4-（sulfooxy）benzoate （6）, （＋）-matairesinol （7）, （-）-pinoresinol （8）, （＋）-lirioresinol-A （9）, and caffeine （10）, among which compound 1 was isolated as a new natural product, and compound 6 was isolated from Theaceae family for the first time. Compounds 3, 4, 5, 7, 8, and 9 were isolated from this species for the first time.展开更多
文摘食用菌在我国具有悠久的食用和药用传统,其主要功效成分为多糖。多糖化学结构复杂,分析方法繁多,不同方法的特点各不相同。近年来多种多糖分析方法用于食用菌多糖的研究,并取得了很多新的研究进展,但目前鲜有针对食用菌多糖分析方法的系统、全面综述。本文系统查阅了Web of Science、PubMed、SciFinder Academic和中国知网等数据库,对近20年有关食用菌多糖提取、分离、纯化和定性、定量分析方法的文献进行总结归纳,并对比分析各种方法的特点,旨在为食用菌多糖的深入研究提供参考。
基金National Natural Science Foundation of China(Grant No.81222054)the Program for New Century Excellent Talents in University from Chinese Ministry of Education(Grant No.NCET-11-0019)
文摘Puerariae Radix （Ge-Gen in Chinese） is a popular traditional Chinese herbal medicine derived from the driedroots ofPueraria lobata （Willd.） Ohwi or P. thomsonii Benth. It shows significant pharmacological activities in the treatment ofcardiovascular diseases, hypertension, and diabetes. This review summarizes progress in the chemistry study of Puerariaspecies, mainly covering the classes of flavonoids, puerosides, coumarins, triterpenoids, and triterpene saponins. Qualitative andquantitative chemical analyses are also covered.
基金New-Century Talent Program, Ministry of Education of China (Grant No.985-2-102-113)National Science Fund for Excellent Young Scholars (Grant No. 81222051)National Key Technology R&D Program "New Drug Innovation" of China (Grant No. 2012ZX09304-005, 2012ZX09301002-002)
文摘The aim of this study was to isolate the chemical constituents of the aerial parts of Polygala tenuifolia Willd. and to determine their antioxidant activities. Ten flavonoids were isolated and purified by silica gel, Sephadex LH-20, and ODS column chromatography, and semi-preparative HPLC. Their structures were elucidated by spectroscopic analysis and identified as isorhamnetin-3-O-13-D-glucopyranoside （1）, isorhamnetin-3-O-13-D-galactopyranoside （2）, quercetin-3-O-13-o-glucopyranosyl （1--＊2）-I^-D-galactopyranoside （3）, quercetin-3-O-13-O-glucopyranosyl （1---~2）-13-D-glucopyranoside （4）, linarin （5）, quercetin-3-O- 13-D-glucopyranoside （6）, 5,7-dihydroxy-8-methxoyflavone-7-O-13-o-glucuronoside （7）, isorhamnetin （8）, kaempferol （9） and quercetin （10）. All these compounds were isolated from this plant for the first time, and compounds 1-5 and 7 were isolated from the genus of Polygala for the first time. The antioxidant activities of the isolated compounds were evaluated by DPPH free radical scavenging assay, and compounds 3, 4, 6, 8, 9 and 10 showed potent antioxidant activities.
基金National Key Technology R&D Program"New Drug Innovation" of China (Grant No. 2012ZX09304-005,2012ZX09301002-002-002)
文摘A new, simple and reliable method using HPLC-UV-ELSD was developed to generate the fingerprint of Ophiopogonis Radix. Homoisoflavonoids and steroidal saponins were determined simultaneously in a single run. A total of 27 Ophiopogonis Radix samples were analyzed, and 18 reference substances were used for the identification of the common peaks. The fingerprint was further analyzed by chemometrics methods including similarity analysis （SA）, hierarchical clustering analysis （HCA） and principal component analysis （PCA）. The results indicated that the combination of chromatographic fingerprint and chemometrics analysis could be used for the geographical differentiation and quality evaluation of Ophiopognnis Radix.
基金National Natural Science Foundation of China(Grant No.21172008 and 21372015)
文摘Twenty-seven flavonoids, including 10 isoflavones, 9 flavanones, 3 dihydrochalcones, 3 flavonols and 2 flavans, were isolated from the whole plant of Tibetan medicine Oxytropis falcate Bunge. Structure elucidation was conducted by extensive spectroscopic analysis, including MS, NMR and CD spectra. Twelve compounds were obtained from this plant for the first time, among which 10 compounds were obtained from the genus of Oxytropis for the first time.
基金Research Fund for the Doctoral Program of Higher Education of China(Grant No.20110001130003)the National Key Technology R&D Program "New Drug Innovation"of China(Grant No.2012ZX09301002-002-002 and 2012ZX09304-005)
文摘A reversed-phase high performance liquid chromatography （RP-HPLC） method with diode array detector （DAD） has been established to simultaneously determine six bioactive compounds in the roots ofllex pubescens, namely tortoside A, （＋）-syringaresinol, ilexsaponin B3, ilexsaponin A1, ilexsaponin B1, ilexgenin A. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phase consisted of acetonitrile and water containing 0.1% （v/v） phosphoric acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm. The six marker constituents were separated well with good linearity （r2〉0.9996）, precision, stability and repeatability. The overall recoveries were in the range of 99.00%-104.52%. Cluster analysis was employed to analyze 15 batches of samples. The result indicated this method provide an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of I. pubescens.
基金National Natural Sciences Foundation of Chin(NS FCGrant Nos.81222051 and 81473106)National Key Technology R&D Program"New Drug Innovation"of China(Gran No s.2012ZX09301002-002-002 and 2012ZX09304-005)
文摘A phytochemical investigation on the stems and rhizomes of Sinomenium acutum led to the isolation of 19 compounds, including three phenanthrenes, six anthraquinones, and ten phenolic derivatives. The structures of the isolated compounds were elucidated by analysis of the MS and NMR spectroscopic data and comparison with the literature. Compounds 1 and 2 are two new natural products, and their 1H and 13C NMR spectral data were fully assigned for the first time, with the help of2D NMR. All of the isolates were obtained from genus Sinomenium for the first time and the phenanthrene and anthraquinone are the structure type first reported from this genus plants.
基金National Science Fund for Excellent Young Scholars(Grant No.81222051)National Key Technology R&D Program “New Drug Innovation” of China(Grant Nos.2012ZX09301002-002-002 and 2012ZX09304-005)
文摘The aim of this study is to perform a phytochemical investigation of the aerial parts of Murraya tetramera Huang. Nine compounds were isolated by silica gel and Sephadex LH-20 chromatography, together with preparative TLC and HPLC methods. By analysis of the MS and NMR spectroscopic data and comparison with those in literature, these nine compounds were identified as 3,3＇-[oxybis（methylene）]bis（9-methoxy-9H-carbazole）（1）, murrastifoline B（2）, murrayaquinone A（3）, 2,4-dimethoxyphenol（4）, 1,2-dimethoxy-4-nitrobenzene（5）, 3-methylanisole（6）,（–）-syringaresinol-4-O-β-D-glucopyranoside（7）, β-sitosterol（8）, and octadecanyl-3-methoxy-4-hydroxylbenzeneacrylate（9）, respectively. Among them, compounds 4–6 and 9 were described from the genus Murraya for the first time, and compounds 2–9 were isolated from M. tetramera for the first time.
基金National Natural Science Foundation of China(Grant No.81172944)
文摘Ten compounds were isolated from Qianliang tea, a kind of dark tea fully fermented from the leaves of Camellia sinensis （L.） O. Kuntze vat. sinensis, and identified .as N-（2-hydroxyphenyl）-2-pyrrolidinone （1）, p-hydroxyacetophenone （2）, salicifoliol （3）, （-）-3-hydroxy-/q-ionone （4）, p-hydroxy ethyl ciunamate （5）, ethyl 4-（sulfooxy）benzoate （6）, （＋）-matairesinol （7）, （-）-pinoresinol （8）, （＋）-lirioresinol-A （9）, and caffeine （10）, among which compound 1 was isolated as a new natural product, and compound 6 was isolated from Theaceae family for the first time. Compounds 3, 4, 5, 7, 8, and 9 were isolated from this species for the first time.
