目的:基于生物信息学方法探讨弱精子症相关的microRNAs(miRNAs)和基因,为弱精子症的诊疗提供新的思路。方法:通过pubmed和web of science筛选文献确定与弱精子症相关的miRNAs,然后通过TargetScan数据库预测其靶基因。在基因表达综合数据...目的:基于生物信息学方法探讨弱精子症相关的microRNAs(miRNAs)和基因,为弱精子症的诊疗提供新的思路。方法:通过pubmed和web of science筛选文献确定与弱精子症相关的miRNAs,然后通过TargetScan数据库预测其靶基因。在基因表达综合数据库(GEO)中选择与弱精子症相关的数据集GSE92578,通过GEO_(2)R软件分析获取差异表达基因(DEGs)。对上述miRNAs的靶基因和分析获得的DEGs取交集,获取最终的DEGs。利用DAVID数据库对上述DEGs进行基因本体分析(GO)和京都基因与基因组百科全书(KEGG)信号通路富集分析。然后,基因STRING数据库进行蛋白互作(PPI)网络分析获得关键基因,再通过cytoscape软件及其插件cytoHubba获取节点基因。结果:通过文献共筛选出7个差异表达的miRNAs和84个差异表达基因。GO富集分析结果,差异基因参与的生物过程(BP)主要包括同源蛋白绑定,蛋白激酶绑定和蛋白质丝氨酸/苏氨酸激酶活动;细胞组成(CC)主要包括细胞质、细胞膜、溶酶体膜和顶体等;分子功能(MF)主要包括RNA聚合酶Ⅱ启动子的转录进行正向调节、蛋白质转移、细胞外基质组织和磷酸化作用的正向调节等。KEGG相关通路涉及PI3K-Akt信号通路、人类乳头瘤病毒感染通路、血小板激活和化学致癌-受体激活等通路。并通过cytoscape获得10个节点基因分别为AKT1、MAPK3、BRD4、DNMT3A、FURIN、LMNB2、COL5A2、COL5A3、COL11A1、COL27A1。结论:本研究获得的miRNAs、hub基因和相关通路,在弱精子症病理程中可能发挥着重要的作用,可为后续机制研究提供参考靶点。展开更多
糖尿病是一种严重威胁人类健康的疾病,其并发症的研究也越来越受到关注,其中糖尿病认知功能障碍(Diabetes cognitive impairment, DCI)是糖尿病非常重要的并发症之一。认知功能障碍的发病机制复杂,且尚未完全阐明,可能与胰岛素抵抗与缺...糖尿病是一种严重威胁人类健康的疾病,其并发症的研究也越来越受到关注,其中糖尿病认知功能障碍(Diabetes cognitive impairment, DCI)是糖尿病非常重要的并发症之一。认知功能障碍的发病机制复杂,且尚未完全阐明,可能与胰岛素抵抗与缺乏、血糖控制不佳、血管疾病、炎症和心理因素等相关。这些因素可导致认知速度减慢、反应时间延长、神经退行性变、脑老化和痴呆等。Diabetes is a serious threat to human health, and the study of its complications is also getting more and more attention. Among them, diabetes cognitive impairment (DCI) is one of the most important complications of diabetes. The pathogenesis of cognitive impairment is complex and not fully understood, and may be related to insulin resistance and deficiency, poor blood sugar control, vascular diseases, inflammation, and psychological factors. These factors can lead to cognitive slowdown, prolonged reaction time, neurodegeneration, brain aging, and dementia.展开更多
糖尿病是一种慢性疾病,通过改变身体使用糖的方式,引起机体一系列的代谢变化。糖尿病是全球性的疾病。据最新报道显示,糖尿病患病人群总数仍在逐年增加,当前全球糖尿病患病率为6.1%,其会导致残疾甚至死亡的恶果。糖尿病心血管并发症是...糖尿病是一种慢性疾病,通过改变身体使用糖的方式,引起机体一系列的代谢变化。糖尿病是全球性的疾病。据最新报道显示,糖尿病患病人群总数仍在逐年增加,当前全球糖尿病患病率为6.1%,其会导致残疾甚至死亡的恶果。糖尿病心血管并发症是糖尿病最严重的慢性并发症之一,发病率高,致残、致死率高,预后差。其确切的发病机制尚且没有明确的定论,仍是需要探讨的问题。目前有研究表明,糖尿病心肌性疾病出现前期,患者的心脏相关功能已经出现异常。临床上通常将超声心动图技术作为检测左心室功能的重要手段。本文对国内外近年来有关国内外糖尿病趋势以及糖尿病心肌病左心室收缩功能障碍的发生背景、发病机制以及辅助检查手段的研究进展予以综述。Diabetes is a chronic disease that causes a series of metabolic changes by changing the way the body uses sugar. Diabetes is a global disease. According to the latest report, the total number of people with diabetes is still increasing year by year, and the current global prevalence of diabetes is 6.1%, which can lead to disability and even death. Cardiovascular complications of diabetes mellitus are one of the most serious chronic complications of diabetes mellitus, with high incidence, high disability, high mortality and poor prognosis. The exact pathogenesis of the disease has not been clearly determined, and it is still a problem that needs to be discussed. At present, studies have shown that in the early stage of diabetic myocardial disease, patients have abnormal heart-related functions. Echocardiography is usually used as an important means to detect left ventricular function in clinic. In this paper, the trend of diabetes at home and abroad in recent years, the background of left ventricular systolic dysfunction in diabetic cardiomyopathy, the pathogenesis and the research progress of auxiliary examination are reviewed.展开更多
术后胃肠功能障碍是继发于手术、创伤的一种胃肠道急性病理生理改变,以肠道粘膜受损、屏障功能破坏及胃肠道运动障碍为主要特征。近年来,加速康复外科在临床中不断应用,加快患者术后胃肠功能恢复也是加速康复外科的重要环节。术后胃肠...术后胃肠功能障碍是继发于手术、创伤的一种胃肠道急性病理生理改变,以肠道粘膜受损、屏障功能破坏及胃肠道运动障碍为主要特征。近年来,加速康复外科在临床中不断应用,加快患者术后胃肠功能恢复也是加速康复外科的重要环节。术后胃肠动力早期恢复将促进患者的全面康复,缩短患者住院时间,减少住院费用,提高医疗资源利用率,减少社会经济负担。Postoperative gastrointestinal dysfunction is a kind of acute pathophysiological changes of the gastrointestinal tract secondary to surgery and trauma, which is characterized by intestinal mucosal damage, barrier function destruction and gastrointestinal motility disorders. In recent years, enhanced recovery after surgery (ERAS) has been applied in clinical practice, and accelerating the recovery of gastrointestinal function after surgery is also an important part of ERAS. Early recovery of gastrointestinal motility after surgery will promote the comprehensive rehabilitation of patients, shorten the length of hospital stay, reduce hospitalization expenses, improve the utilization of medical resources, and reduce the social and economic burden.展开更多
文摘目的:基于生物信息学方法探讨弱精子症相关的microRNAs(miRNAs)和基因,为弱精子症的诊疗提供新的思路。方法:通过pubmed和web of science筛选文献确定与弱精子症相关的miRNAs,然后通过TargetScan数据库预测其靶基因。在基因表达综合数据库(GEO)中选择与弱精子症相关的数据集GSE92578,通过GEO_(2)R软件分析获取差异表达基因(DEGs)。对上述miRNAs的靶基因和分析获得的DEGs取交集,获取最终的DEGs。利用DAVID数据库对上述DEGs进行基因本体分析(GO)和京都基因与基因组百科全书(KEGG)信号通路富集分析。然后,基因STRING数据库进行蛋白互作(PPI)网络分析获得关键基因,再通过cytoscape软件及其插件cytoHubba获取节点基因。结果:通过文献共筛选出7个差异表达的miRNAs和84个差异表达基因。GO富集分析结果,差异基因参与的生物过程(BP)主要包括同源蛋白绑定,蛋白激酶绑定和蛋白质丝氨酸/苏氨酸激酶活动;细胞组成(CC)主要包括细胞质、细胞膜、溶酶体膜和顶体等;分子功能(MF)主要包括RNA聚合酶Ⅱ启动子的转录进行正向调节、蛋白质转移、细胞外基质组织和磷酸化作用的正向调节等。KEGG相关通路涉及PI3K-Akt信号通路、人类乳头瘤病毒感染通路、血小板激活和化学致癌-受体激活等通路。并通过cytoscape获得10个节点基因分别为AKT1、MAPK3、BRD4、DNMT3A、FURIN、LMNB2、COL5A2、COL5A3、COL11A1、COL27A1。结论:本研究获得的miRNAs、hub基因和相关通路,在弱精子症病理程中可能发挥着重要的作用,可为后续机制研究提供参考靶点。
文摘糖尿病是一种严重威胁人类健康的疾病,其并发症的研究也越来越受到关注,其中糖尿病认知功能障碍(Diabetes cognitive impairment, DCI)是糖尿病非常重要的并发症之一。认知功能障碍的发病机制复杂,且尚未完全阐明,可能与胰岛素抵抗与缺乏、血糖控制不佳、血管疾病、炎症和心理因素等相关。这些因素可导致认知速度减慢、反应时间延长、神经退行性变、脑老化和痴呆等。Diabetes is a serious threat to human health, and the study of its complications is also getting more and more attention. Among them, diabetes cognitive impairment (DCI) is one of the most important complications of diabetes. The pathogenesis of cognitive impairment is complex and not fully understood, and may be related to insulin resistance and deficiency, poor blood sugar control, vascular diseases, inflammation, and psychological factors. These factors can lead to cognitive slowdown, prolonged reaction time, neurodegeneration, brain aging, and dementia.
文摘糖尿病是一种慢性疾病,通过改变身体使用糖的方式,引起机体一系列的代谢变化。糖尿病是全球性的疾病。据最新报道显示,糖尿病患病人群总数仍在逐年增加,当前全球糖尿病患病率为6.1%,其会导致残疾甚至死亡的恶果。糖尿病心血管并发症是糖尿病最严重的慢性并发症之一,发病率高,致残、致死率高,预后差。其确切的发病机制尚且没有明确的定论,仍是需要探讨的问题。目前有研究表明,糖尿病心肌性疾病出现前期,患者的心脏相关功能已经出现异常。临床上通常将超声心动图技术作为检测左心室功能的重要手段。本文对国内外近年来有关国内外糖尿病趋势以及糖尿病心肌病左心室收缩功能障碍的发生背景、发病机制以及辅助检查手段的研究进展予以综述。Diabetes is a chronic disease that causes a series of metabolic changes by changing the way the body uses sugar. Diabetes is a global disease. According to the latest report, the total number of people with diabetes is still increasing year by year, and the current global prevalence of diabetes is 6.1%, which can lead to disability and even death. Cardiovascular complications of diabetes mellitus are one of the most serious chronic complications of diabetes mellitus, with high incidence, high disability, high mortality and poor prognosis. The exact pathogenesis of the disease has not been clearly determined, and it is still a problem that needs to be discussed. At present, studies have shown that in the early stage of diabetic myocardial disease, patients have abnormal heart-related functions. Echocardiography is usually used as an important means to detect left ventricular function in clinic. In this paper, the trend of diabetes at home and abroad in recent years, the background of left ventricular systolic dysfunction in diabetic cardiomyopathy, the pathogenesis and the research progress of auxiliary examination are reviewed.
文摘术后胃肠功能障碍是继发于手术、创伤的一种胃肠道急性病理生理改变,以肠道粘膜受损、屏障功能破坏及胃肠道运动障碍为主要特征。近年来,加速康复外科在临床中不断应用,加快患者术后胃肠功能恢复也是加速康复外科的重要环节。术后胃肠动力早期恢复将促进患者的全面康复,缩短患者住院时间,减少住院费用,提高医疗资源利用率,减少社会经济负担。Postoperative gastrointestinal dysfunction is a kind of acute pathophysiological changes of the gastrointestinal tract secondary to surgery and trauma, which is characterized by intestinal mucosal damage, barrier function destruction and gastrointestinal motility disorders. In recent years, enhanced recovery after surgery (ERAS) has been applied in clinical practice, and accelerating the recovery of gastrointestinal function after surgery is also an important part of ERAS. Early recovery of gastrointestinal motility after surgery will promote the comprehensive rehabilitation of patients, shorten the length of hospital stay, reduce hospitalization expenses, improve the utilization of medical resources, and reduce the social and economic burden.
