OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided in...OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided into an experimental group transfected with a recombinant pcDNA3-hNIS plasmid and a control group transfected only with a pcDNA3 plasmid. The recombinant plasmid vector encoding the hNIS gene （pcDNA3-hNIS） was amplified, purified and identified. The hNIS gene was followed by DNA sequencing. A Western blot and an immunohistochemical assay were applied to detect the hNIS protein expression in the transfected human lung A549 cancer cells. RESULTS Restriction enzyme digestion and DNA sequencing results showed the size and direction of the inserted gene in the recombinant pcD- NA3-hNIS plasmid was correct. The Western blot method and immunohistochemical analysis showed a positive NIS protein expression in the experimental group. The NIS protein was detected mainly in the cell membranes showing a positive rate up to 70.6% with no expression of the NIS protein in the control group. There was a significant difference between two groups （P=0.000）. CONCLUSION The hNIS gene was transfected effectively into human lung A549 cancer cells mediated by Lipofectamine 2000, and was expressed with its protein in vitro.展开更多
目的综合评价三类主动脉弓部手术的并发症发生率、死亡率及生存率,为弓部夹层手术提供相关临床依据。方法利用计算机搜索在2004-2014年发表在Pub Med、Web of science、西文医学生物期刊三个数据库中关于主动脉夹层弓部相关手术的文献,...目的综合评价三类主动脉弓部手术的并发症发生率、死亡率及生存率,为弓部夹层手术提供相关临床依据。方法利用计算机搜索在2004-2014年发表在Pub Med、Web of science、西文医学生物期刊三个数据库中关于主动脉夹层弓部相关手术的文献,采用MINORS条目对文献进行质量评价。相关数据通过Stata10.0统计软件进行分析处理。统计时采用并发症的发生率、生存率及95%可信区间(CI)进行结果描述。结果本Meta分析纳入文献共33篇,每篇文献病例数在21～411例之间,所有文献均采用回顾性研究。其中开放手术组7篇,支架组16篇,杂交手术组10篇。开放手术组:总NS并发症发生率为5.4%(95%CI:3.9%～7.0%);二次手术率为7.8%(95%CI:5.8%～9.7%);1年生存率为91.1%(95%CI:86.6%～95.7%);5年生存率为89.5%(95%CI:86.5%～92.6%);支架组:二次手术率为5.0%(95%CI:3.0%～7.0%);术后30d内死亡率为3.2%(95%CI:2.1%～4.2%);支架内漏发生率为64%(95%CI:5.0%～7.8%);1年生存率为91.3%(95%CI:89.4%～93.2%);5年生存率为79.1%(95%CI:76.2%～82.0%);杂交组:二次手术率为7.6%(95%CI:3.9%～11.2%);术后30d内死亡率为7.4%(95%CI:5.2%～9.7%);支架内漏率为6.9%(95%CI:4.7%～9.1%);1年生存率为84.9%(95%CI:81.6%～88.3%);5年生存率为73.9%(95%CI:68.3%～79.5%)。三类手术总的并发症发生率及生存率的合并:总NS并发症发生率为5.5%(95%CI:4.6%～6.5%);术后30d内死亡率为4.2%(95%CI:3.4%～5.0%);支架组和杂交组术后支架内漏发生率为6.5%(95%CI:5.3%-7.7%);总的1年生存率为88.8%(95%CI:86.5%～91.1%);5年生存率为79.4%(95%CI:74.4%～84.5%)。结论开放手术对夹层的治疗效果显著,有较高的远期生存率,适合年龄较轻、基础疾病少、主动脉瓣及弓部病变严重的患者。介入手术创伤小,住院时间短,早期疗效好,尤其适合高龄、基础疾病多,有足够锚定区的弓部夹层患者。杂交手术是对于高龄和多器官功能障碍而不能耐受体外循环或深低温停循环手术患者较为安全的手术方式,适合全身情况较差、锚定区不足的弓部夹层患者。展开更多
Objective： To observe the relationship between non-small cell lung cancer with neuroendrocrine differentiation （NSCLC-NE） and patients＇ postoperative survival. Methods： During April 1997 to April 1999, 98 cases o...Objective： To observe the relationship between non-small cell lung cancer with neuroendrocrine differentiation （NSCLC-NE） and patients＇ postoperative survival. Methods： During April 1997 to April 1999, 98 cases of hlng cancer were surgically treated. The tumor specimens of the patients were stained by NE markers, i.e. neuron specific enolase （NSE） and synaptophysin （SY）. The intensity of NE markers reaction was divided as ＂＋＂. ＂＋＋＂. ＂＋＋＋＂ scale groups. The same specimens were also examined under an electron microscope for the specific neuroendocrine granules. All enrolled patients were followed up for 36 months, and the longest follow-up time was 60 months. The COX proportional hazard model multivariate analysis was applied to observe the relationship between the NSCLE-NE and the patients＇ postoperative survival. Results： In 91 cases of NSCLC, 63.7% （58/91） were positive for NE stain reaction. Among them, 59.3% （54/91） were positive for NSE and 24.1% （22/91） for SY. 48.4% （44/91） were considered as NSCLC-NE by the combination of NE inarker stain reaction and electron microscopic examination. COX proportional hazard model lnnltivariate analysis showed that the NSCLC-NE patients＇ survival was significantly shortened （P=0.048）. The following factors were related to NSCLC-NE patients＇ survival： lung cancer cell differentiation （P=0.006）, clinical lung cancer stage （P=0.001）, the NE markers reaction （P=0.054）. Conclusion： NSCLE-NE is significantly related to the cancer cell differentiation and the patients＇ postoperative survival. The NE markers should be applied clinically as one of prognostic factors to evaluate the postoperative survival of NSCLC patients.展开更多
1病例资料患者男性,63岁,因＂进食不畅感十余天＂于2016年10月29日收入南通大学附属医院胸外科住院。患者无恶心、呕吐,无反酸、嗳气,无胸痛、胸闷,无呼吸困难,无声音嘶哑,无饮水呛咳。既往史及个人史：有酗酒史,饮酒40余年,每日饮黄酒...1病例资料患者男性,63岁,因＂进食不畅感十余天＂于2016年10月29日收入南通大学附属医院胸外科住院。患者无恶心、呕吐,无反酸、嗳气,无胸痛、胸闷,无呼吸困难,无声音嘶哑,无饮水呛咳。既往史及个人史：有酗酒史,饮酒40余年,每日饮黄酒量≥1 000 m L,曾戒酒后出现失眠、语无伦次等精神症状,至当地精神病医院就治后症状消失,出院后复饮.展开更多
文摘OBJECTIVE To examine the possibility of human sodium iodide symporter （hNIS） protein expression in lung cancer cells. METHODS Human lung A549 cancer cells were thawed and cultured in vitro. The cells were divided into an experimental group transfected with a recombinant pcDNA3-hNIS plasmid and a control group transfected only with a pcDNA3 plasmid. The recombinant plasmid vector encoding the hNIS gene （pcDNA3-hNIS） was amplified, purified and identified. The hNIS gene was followed by DNA sequencing. A Western blot and an immunohistochemical assay were applied to detect the hNIS protein expression in the transfected human lung A549 cancer cells. RESULTS Restriction enzyme digestion and DNA sequencing results showed the size and direction of the inserted gene in the recombinant pcD- NA3-hNIS plasmid was correct. The Western blot method and immunohistochemical analysis showed a positive NIS protein expression in the experimental group. The NIS protein was detected mainly in the cell membranes showing a positive rate up to 70.6% with no expression of the NIS protein in the control group. There was a significant difference between two groups （P=0.000）. CONCLUSION The hNIS gene was transfected effectively into human lung A549 cancer cells mediated by Lipofectamine 2000, and was expressed with its protein in vitro.
文摘目的综合评价三类主动脉弓部手术的并发症发生率、死亡率及生存率,为弓部夹层手术提供相关临床依据。方法利用计算机搜索在2004-2014年发表在Pub Med、Web of science、西文医学生物期刊三个数据库中关于主动脉夹层弓部相关手术的文献,采用MINORS条目对文献进行质量评价。相关数据通过Stata10.0统计软件进行分析处理。统计时采用并发症的发生率、生存率及95%可信区间(CI)进行结果描述。结果本Meta分析纳入文献共33篇,每篇文献病例数在21～411例之间,所有文献均采用回顾性研究。其中开放手术组7篇,支架组16篇,杂交手术组10篇。开放手术组:总NS并发症发生率为5.4%(95%CI:3.9%～7.0%);二次手术率为7.8%(95%CI:5.8%～9.7%);1年生存率为91.1%(95%CI:86.6%～95.7%);5年生存率为89.5%(95%CI:86.5%～92.6%);支架组:二次手术率为5.0%(95%CI:3.0%～7.0%);术后30d内死亡率为3.2%(95%CI:2.1%～4.2%);支架内漏发生率为64%(95%CI:5.0%～7.8%);1年生存率为91.3%(95%CI:89.4%～93.2%);5年生存率为79.1%(95%CI:76.2%～82.0%);杂交组:二次手术率为7.6%(95%CI:3.9%～11.2%);术后30d内死亡率为7.4%(95%CI:5.2%～9.7%);支架内漏率为6.9%(95%CI:4.7%～9.1%);1年生存率为84.9%(95%CI:81.6%～88.3%);5年生存率为73.9%(95%CI:68.3%～79.5%)。三类手术总的并发症发生率及生存率的合并:总NS并发症发生率为5.5%(95%CI:4.6%～6.5%);术后30d内死亡率为4.2%(95%CI:3.4%～5.0%);支架组和杂交组术后支架内漏发生率为6.5%(95%CI:5.3%-7.7%);总的1年生存率为88.8%(95%CI:86.5%～91.1%);5年生存率为79.4%(95%CI:74.4%～84.5%)。结论开放手术对夹层的治疗效果显著,有较高的远期生存率,适合年龄较轻、基础疾病少、主动脉瓣及弓部病变严重的患者。介入手术创伤小,住院时间短,早期疗效好,尤其适合高龄、基础疾病多,有足够锚定区的弓部夹层患者。杂交手术是对于高龄和多器官功能障碍而不能耐受体外循环或深低温停循环手术患者较为安全的手术方式,适合全身情况较差、锚定区不足的弓部夹层患者。
文摘Objective： To observe the relationship between non-small cell lung cancer with neuroendrocrine differentiation （NSCLC-NE） and patients＇ postoperative survival. Methods： During April 1997 to April 1999, 98 cases of hlng cancer were surgically treated. The tumor specimens of the patients were stained by NE markers, i.e. neuron specific enolase （NSE） and synaptophysin （SY）. The intensity of NE markers reaction was divided as ＂＋＂. ＂＋＋＂. ＂＋＋＋＂ scale groups. The same specimens were also examined under an electron microscope for the specific neuroendocrine granules. All enrolled patients were followed up for 36 months, and the longest follow-up time was 60 months. The COX proportional hazard model multivariate analysis was applied to observe the relationship between the NSCLE-NE and the patients＇ postoperative survival. Results： In 91 cases of NSCLC, 63.7% （58/91） were positive for NE stain reaction. Among them, 59.3% （54/91） were positive for NSE and 24.1% （22/91） for SY. 48.4% （44/91） were considered as NSCLC-NE by the combination of NE inarker stain reaction and electron microscopic examination. COX proportional hazard model lnnltivariate analysis showed that the NSCLC-NE patients＇ survival was significantly shortened （P=0.048）. The following factors were related to NSCLC-NE patients＇ survival： lung cancer cell differentiation （P=0.006）, clinical lung cancer stage （P=0.001）, the NE markers reaction （P=0.054）. Conclusion： NSCLE-NE is significantly related to the cancer cell differentiation and the patients＇ postoperative survival. The NE markers should be applied clinically as one of prognostic factors to evaluate the postoperative survival of NSCLC patients.
文摘1病例资料患者男性,63岁,因＂进食不畅感十余天＂于2016年10月29日收入南通大学附属医院胸外科住院。患者无恶心、呕吐,无反酸、嗳气,无胸痛、胸闷,无呼吸困难,无声音嘶哑,无饮水呛咳。既往史及个人史：有酗酒史,饮酒40余年,每日饮黄酒量≥1 000 m L,曾戒酒后出现失眠、语无伦次等精神症状,至当地精神病医院就治后症状消失,出院后复饮.
