目的:探索硫糖铝对小鼠放射性口腔黏膜炎(radiation-induced oral mucositis;RIOM)的治疗效果及其可能机制。材料和方法:建立小鼠RIOM模型,从小鼠辐照完成后至处死小鼠期间,每日使用硫糖铝局部涂抹小鼠舌背,观察溃疡情况,并行组织病理学...目的:探索硫糖铝对小鼠放射性口腔黏膜炎(radiation-induced oral mucositis;RIOM)的治疗效果及其可能机制。材料和方法:建立小鼠RIOM模型,从小鼠辐照完成后至处死小鼠期间,每日使用硫糖铝局部涂抹小鼠舌背,观察溃疡情况,并行组织病理学和WB检测。结果:18 Gy γ射线大剂量一次性照射头颈部后第9天小鼠舌部出现明显的溃疡。与单纯辐照组相比,硫糖铝处理组小鼠舌背溃疡面积较小,舌背黏膜基底层细胞更多,组织内促凋亡蛋白Bax和Caspase-3水平显著降低,抗凋亡蛋白Bcl-2水平显著升高。结论:使用硫糖铝治疗能改善RIOM小鼠的舌背黏膜情况,推测可能是通过形成局部屏障、促进细胞增殖、抗细胞凋亡、清除自由基等方面作用从而减轻口腔黏膜局部炎症,促进黏膜炎愈合。Purpose: To investigate the therapeutic effects of sucralfate on radiation-induced oral mucositis (RIOM) in mice and explore its potential mechanisms. Materials and Methods: A mouse model of RIOM was established. From the completion of irradiation until euthanasia, sucralfate was topically applied to the dorsal tongue of mice daily. Ulcer conditions were observed, and histopathological and Western blot (WB) analyses were performed. Results: On the 9th day after a single high-dose (18 Gy) γ-ray irradiation of the head and neck, significant ulcers appeared on the tongues of mice. Compared to the irradiation-only group, the sucralfate-treated group exhibited smaller ulcer areas on the dorsal tongue, a greater number of basal layer cells in the dorsal tongue mucosa, significantly reduced levels of pro-apoptotic proteins Bax and Caspase-3, and significantly increased levels of the anti-apoptotic protein Bcl-2. Conclusion: Treatment with sucralfate improved the mucosal condition of the dorsal tongue in RIOM mice. It is hypothesized that sucralfate may alleviate local inflammation and promote mucosal healing through mechanisms such as forming a local barrier, promoting cell proliferation, inhibiting apoptosis, and scavenging free radicals.展开更多
微/纳米马达在生物医学中的应用在近年来引起了广泛的研究,结合囊泡的微/纳米马达在生物医学领域具有极大的应用潜力,由于微/纳米马达的自驱动力以及囊泡本身具有的良好的生物相容性使其在药物递送、光热疗法、解毒等领域均有所应用。...微/纳米马达在生物医学中的应用在近年来引起了广泛的研究,结合囊泡的微/纳米马达在生物医学领域具有极大的应用潜力,由于微/纳米马达的自驱动力以及囊泡本身具有的良好的生物相容性使其在药物递送、光热疗法、解毒等领域均有所应用。本文回顾了结合囊泡的微/纳米马达的制备方式,以及结合不同类型囊泡的微纳米马达的驱动力、结合方式及应用。总而言之,结合囊泡的微/纳米马达有望推进微/纳米马达在生物医学领域的应用,并促进其临床转化。The application of micro/nanomotors in biomedicine has garnered widespread attention in recent years. The integration of vesicles with micro/nanomotors holds significant potential in the biomedical field. Due to the self-propelling nature of micro/nanomotors and the excellent biocompatibility of vesicles, they have been applied in areas such as drug delivery, photothermal therapy, and detoxification. This article reviews the preparation methods of micro/nanomotors combined with vesicles, as well as the driving forces, coupling methods, and applications of micro/nanomotors integrated with different types of vesicles. In summary, micro/nanomotors combined with vesicles are expected to advance the application of micro/nanomotors in biomedicine and promote their clinical translation.展开更多
人体微生物群落的平衡对健康至关重要,其失衡与衰老存在密切关联。衰老过程常伴随微生物群落组成与功能的改变,引发机体慢性炎症,进而影响免疫系统,加速衰老及相关系统性疾病的发生发展。近年来,借助先进的分子生物学技术,该领域研究不...人体微生物群落的平衡对健康至关重要,其失衡与衰老存在密切关联。衰老过程常伴随微生物群落组成与功能的改变,引发机体慢性炎症,进而影响免疫系统,加速衰老及相关系统性疾病的发生发展。近年来,借助先进的分子生物学技术,该领域研究不断深入,益生菌、益生元及粪菌移植等干预措施也展现出一定潜力。然而,在精准干预策略、长期效果评估及安全性保障等方面仍面临挑战,后续需开展更多研究,助力健康老龄化目标的实现。The equilibrium of the human microbiota community plays a pivotal role in maintaining health, and its perturbation exhibits a profound correlation with the aging process. The aging process is often accompanied by changes in the composition and function of the microbiota, triggering chronic inflammation in the body, which in turn affects the immune system and accelerates the occurrence and development of aging and related systemic diseases. In recent years, with the help of advanced molecular biology techniques, research in this field has been continuously deepening, and intervention measures such as probiotics, prebiotics, and fecal microbiota transplantation have also shown certain potential. However, challenges still remain in terms of precise intervention strategies, long-term effect evaluation, and safety assurance. More research needs to be carried out in the future to contribute to the achievement of the goal of healthy aging.展开更多
随着全球人口老龄化趋势的加剧,主要波及老年人群认知障碍的发病率在全球不同地区均呈现上升趋势,这一现象与老年人免疫功能的减退和口腔卫生维护能力的下降密切相关,进而导致口腔功能受损和牙菌斑积累增加。本文阐述了衰老时口腔结构...随着全球人口老龄化趋势的加剧,主要波及老年人群认知障碍的发病率在全球不同地区均呈现上升趋势,这一现象与老年人免疫功能的减退和口腔卫生维护能力的下降密切相关,进而导致口腔功能受损和牙菌斑积累增加。本文阐述了衰老时口腔结构和功能的改变、微生物的变化、口腔疾病的发生发展特点。探讨了口腔健康不良对认知功能影响的途径。旨在强调重视老年人口腔健康对预防和延缓衰老过程中认知障碍发生发展的重要性,为后续进一步研究及干预策略制定提供参考依据。With the intensification of the global population aging trend, the incidence of cognitive impairment mainly affecting the elderly population is increasing in different regions of the world, which is closely related to the decline of immune function and oral health maintenance ability of the elderly, which in turn leads to the impairment of oral function and the increase of plaque accumulation. This article expounds the changes in oral structure and function, the changes in microorganisms, and the occurrence and development characteristics of oral diseases during aging. The pathways of the impact of poor oral health on cognitive function were explored. The purpose of this study is to emphasize the importance of paying attention to the oral health of the elderly to prevent and delay the occurrence and development of cognitive impairment in the aging process, and to provide a reference for further research and the formulation of intervention strategies.展开更多
文摘目的:探索硫糖铝对小鼠放射性口腔黏膜炎(radiation-induced oral mucositis;RIOM)的治疗效果及其可能机制。材料和方法:建立小鼠RIOM模型,从小鼠辐照完成后至处死小鼠期间,每日使用硫糖铝局部涂抹小鼠舌背,观察溃疡情况,并行组织病理学和WB检测。结果:18 Gy γ射线大剂量一次性照射头颈部后第9天小鼠舌部出现明显的溃疡。与单纯辐照组相比,硫糖铝处理组小鼠舌背溃疡面积较小,舌背黏膜基底层细胞更多,组织内促凋亡蛋白Bax和Caspase-3水平显著降低,抗凋亡蛋白Bcl-2水平显著升高。结论:使用硫糖铝治疗能改善RIOM小鼠的舌背黏膜情况,推测可能是通过形成局部屏障、促进细胞增殖、抗细胞凋亡、清除自由基等方面作用从而减轻口腔黏膜局部炎症,促进黏膜炎愈合。Purpose: To investigate the therapeutic effects of sucralfate on radiation-induced oral mucositis (RIOM) in mice and explore its potential mechanisms. Materials and Methods: A mouse model of RIOM was established. From the completion of irradiation until euthanasia, sucralfate was topically applied to the dorsal tongue of mice daily. Ulcer conditions were observed, and histopathological and Western blot (WB) analyses were performed. Results: On the 9th day after a single high-dose (18 Gy) γ-ray irradiation of the head and neck, significant ulcers appeared on the tongues of mice. Compared to the irradiation-only group, the sucralfate-treated group exhibited smaller ulcer areas on the dorsal tongue, a greater number of basal layer cells in the dorsal tongue mucosa, significantly reduced levels of pro-apoptotic proteins Bax and Caspase-3, and significantly increased levels of the anti-apoptotic protein Bcl-2. Conclusion: Treatment with sucralfate improved the mucosal condition of the dorsal tongue in RIOM mice. It is hypothesized that sucralfate may alleviate local inflammation and promote mucosal healing through mechanisms such as forming a local barrier, promoting cell proliferation, inhibiting apoptosis, and scavenging free radicals.
文摘微/纳米马达在生物医学中的应用在近年来引起了广泛的研究,结合囊泡的微/纳米马达在生物医学领域具有极大的应用潜力,由于微/纳米马达的自驱动力以及囊泡本身具有的良好的生物相容性使其在药物递送、光热疗法、解毒等领域均有所应用。本文回顾了结合囊泡的微/纳米马达的制备方式,以及结合不同类型囊泡的微纳米马达的驱动力、结合方式及应用。总而言之,结合囊泡的微/纳米马达有望推进微/纳米马达在生物医学领域的应用,并促进其临床转化。The application of micro/nanomotors in biomedicine has garnered widespread attention in recent years. The integration of vesicles with micro/nanomotors holds significant potential in the biomedical field. Due to the self-propelling nature of micro/nanomotors and the excellent biocompatibility of vesicles, they have been applied in areas such as drug delivery, photothermal therapy, and detoxification. This article reviews the preparation methods of micro/nanomotors combined with vesicles, as well as the driving forces, coupling methods, and applications of micro/nanomotors integrated with different types of vesicles. In summary, micro/nanomotors combined with vesicles are expected to advance the application of micro/nanomotors in biomedicine and promote their clinical translation.
文摘人体微生物群落的平衡对健康至关重要,其失衡与衰老存在密切关联。衰老过程常伴随微生物群落组成与功能的改变,引发机体慢性炎症,进而影响免疫系统,加速衰老及相关系统性疾病的发生发展。近年来,借助先进的分子生物学技术,该领域研究不断深入,益生菌、益生元及粪菌移植等干预措施也展现出一定潜力。然而,在精准干预策略、长期效果评估及安全性保障等方面仍面临挑战,后续需开展更多研究,助力健康老龄化目标的实现。The equilibrium of the human microbiota community plays a pivotal role in maintaining health, and its perturbation exhibits a profound correlation with the aging process. The aging process is often accompanied by changes in the composition and function of the microbiota, triggering chronic inflammation in the body, which in turn affects the immune system and accelerates the occurrence and development of aging and related systemic diseases. In recent years, with the help of advanced molecular biology techniques, research in this field has been continuously deepening, and intervention measures such as probiotics, prebiotics, and fecal microbiota transplantation have also shown certain potential. However, challenges still remain in terms of precise intervention strategies, long-term effect evaluation, and safety assurance. More research needs to be carried out in the future to contribute to the achievement of the goal of healthy aging.
文摘随着全球人口老龄化趋势的加剧,主要波及老年人群认知障碍的发病率在全球不同地区均呈现上升趋势,这一现象与老年人免疫功能的减退和口腔卫生维护能力的下降密切相关,进而导致口腔功能受损和牙菌斑积累增加。本文阐述了衰老时口腔结构和功能的改变、微生物的变化、口腔疾病的发生发展特点。探讨了口腔健康不良对认知功能影响的途径。旨在强调重视老年人口腔健康对预防和延缓衰老过程中认知障碍发生发展的重要性,为后续进一步研究及干预策略制定提供参考依据。With the intensification of the global population aging trend, the incidence of cognitive impairment mainly affecting the elderly population is increasing in different regions of the world, which is closely related to the decline of immune function and oral health maintenance ability of the elderly, which in turn leads to the impairment of oral function and the increase of plaque accumulation. This article expounds the changes in oral structure and function, the changes in microorganisms, and the occurrence and development characteristics of oral diseases during aging. The pathways of the impact of poor oral health on cognitive function were explored. The purpose of this study is to emphasize the importance of paying attention to the oral health of the elderly to prevent and delay the occurrence and development of cognitive impairment in the aging process, and to provide a reference for further research and the formulation of intervention strategies.
