目的:对比研究肾上腺髓质素(Adrenomedullin ADM)对自发性高血压大鼠(spontaneously hypertensive rat SHR)心脏重构的影响及与ERK1/2活化的关系。方法:4周龄雄性SHR随机分为SHR组和SHR-T组,同周龄WKY大鼠作为正常对照。SHR-T组皮下注射...目的:对比研究肾上腺髓质素(Adrenomedullin ADM)对自发性高血压大鼠(spontaneously hypertensive rat SHR)心脏重构的影响及与ERK1/2活化的关系。方法:4周龄雄性SHR随机分为SHR组和SHR-T组,同周龄WKY大鼠作为正常对照。SHR-T组皮下注射ADM(每周5天,1.0nmol/(kg.d)),SHR组皮下注射等体积生理盐水,WKY组不作处理。在第4、8、16和24周龄时间点分别处死动物(每组4只),提取心脏做H.E染色和P-ERK1/2免疫组化染色。结果:SHR组和SHR-T组从8周龄开始血压显著升高,明显高于WKY组(P<0.01),ADM治疗至24周龄时,血压明显低于SHR组;随着大鼠周龄增加,SHR组和SHR-T组大鼠心脏/体重比值逐渐增大。16周龄后,SHR组和SHR-T组的心脏/体重比值大于WKY组(P<0.05),24周龄SHR-T组大鼠的心脏/体重比值小于SHR组(P<0.05);随着大鼠周龄增加,SHR和SHR-T组大鼠相对左心室壁厚度呈增加趋势,24周龄SHR组大鼠相对左心室壁厚度大于同周龄WKY组(P<0.05)。SHR-T组大鼠相对左心室壁厚度在16周龄后增幅放缓,24周龄时小于SHR组(P<0.05);随着大鼠周龄的增加p-ERK1/2在大鼠心肌中的阳性表达逐渐增加,24周龄SHR组大鼠心脏p-ERK1/2的表达率显著高于WKY组(P<0.01),24周龄SHR-T组大鼠心脏p-ERK1/2的表达率显著低于SHR组(P<0.05)。结论:ADM可一定程度减缓自发性高血压大鼠血压的升高和心脏重构,p-ERK1/2的表达量的降低可能参与了ADM发挥其保护作用。展开更多
目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞机化后基因表达的差异。方法:40只C57小鼠随机分为对照组(CNC组12只)及癫痫组PTZ (CPTZ组(戊四氮pentylenetetrazole,PTZ)组28只),CNC组给予生理盐水,CPTZ组给予戊四氮,隔日腹腔注射,共28 ...目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞机化后基因表达的差异。方法:40只C57小鼠随机分为对照组(CNC组12只)及癫痫组PTZ (CPTZ组(戊四氮pentylenetetrazole,PTZ)组28只),CNC组给予生理盐水,CPTZ组给予戊四氮,隔日腹腔注射,共28 d。诱导癫痫过程中,观察小鼠行为,癫痫发作级别、持续时间及结局,实验结束后取材,进行相应转录组测序分析。样本的MethylationEPIC BeadChip实验及数据分析由欧易生物技术有限公司(中国上海)进行。结果:转录组相关测序显示癫痫发作改变小鼠脑组织海马区小胶质细胞M2型的基因表达,其中表达上调基因245个,表达下调基因88个。结论:癫痫发作改变小鼠海马区小胶质细胞的基因异常表达。Objective: To investigate the effects of epilepsy on the mechanized gene expression of microglia in hippocampus of C57 mouse. Methods: 40 C57 mice were randomly divided into control group (12 in CNC group) and PTZ group (28 in CPTZ group). The CNC group was given normal saline, and the CPTZ group was given pentatetrazole by intraperitoneal injection every other day for a total of 28 days. During the process of inducing epilepsy, the behavior, seizure level, duration and outcome of the mice were observed. After the experiment, the samples were collected and analyzed by transcriptome sequencing. Sample MethylationEPIC BeadChip experiments and data analysis were performed by OYI Biotechnology Co., Ltd. (Shanghai, China). Results: Transcriptome-related sequencing showed that epileptic seizure changed the expression of M2 type of microglia in the hippocampus of mouse brain tissue, including 245 up-regulated genes and 88 down-regulated genes. Conclusion: Epileptic seizure changes the abnormal gene expression of microglia in hippocampus of mouse.展开更多
目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞极化后基因的富集分析。方法:将40只C57小鼠利用随机分组的方法随机分为对照组(CNC组12只)和癫痫组(OPTZ组28只)。CNC组每隔一天腹腔注射生理盐水,OPTZ组每隔一天腹腔注射戊四唑,共28天。...目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞极化后基因的富集分析。方法:将40只C57小鼠利用随机分组的方法随机分为对照组(CNC组12只)和癫痫组(OPTZ组28只)。CNC组每隔一天腹腔注射生理盐水,OPTZ组每隔一天腹腔注射戊四唑,共28天。在诱导癫痫的实验过程中,观察小鼠的日常行为、癫痫发作程度、癫痫持续时间和癫痫发作后的结局。实验结束后采集样本,进行转录组测序分析。OE生物技术有限公司(上海,中国)负责样品Methylation EPIC Bead Chip实验结果和数据分析的进行。结果:转录组相关测序显示癫痫发作改变小鼠脑组织海马区小胶质细胞M2型的基因表达,其中表达上调基因245个,表达下调基因88个,GO富集分析显示上调基因富集于影响小胶质细胞生长和增殖的通路。结论:癫痫发作改变小鼠海马区小胶质细胞的基因表达且富集于抑制小胶质细胞生长发育通路。Objective: To study the gene enrichment of microglia in hippocampal region of C57 mouse brain after polarization induced by epilepsy. Methods: 40 C57 mice were randomly divided into control group (12 in CNC group) and epilepsy group (28 in OPTZ group). The CNC group was intraperitoneally injected with normal saline every other day, and the OPTZ group was intraperitoneally injected with pentatetrazole every other day for 28 days. In the course of the experiment to induce epilepsy, the daily behavior of the mouse, the degree of seizure, the duration of seizure and the outcome after seizure were observed. After the experiment, the samples were collected and analyzed by transcriptome sequencing. OE Biotechnology Co. Ltd. (Shanghai, China) was responsible for the experimental results and data analysis of the sample, Methylation EPIC Bead Chip. Results: Transcriptome sequencing showed that seizures changed the M2 gene expression of microglia in the hippocampus of mouse brain tissue, including 245 up-regulated genes and 88 down-regulated genes. GO enrichment analysis showed that up-regulated genes were enriched in the pathway affecting the growth and proliferation of microglia. Conclusion: Epileptic seizure changes the gene expression of microglia in the hippocampus of mouse and is concentrated in the pathway that inhibits the growth and development of microglia.展开更多
文摘目的:对比研究肾上腺髓质素(Adrenomedullin ADM)对自发性高血压大鼠(spontaneously hypertensive rat SHR)心脏重构的影响及与ERK1/2活化的关系。方法:4周龄雄性SHR随机分为SHR组和SHR-T组,同周龄WKY大鼠作为正常对照。SHR-T组皮下注射ADM(每周5天,1.0nmol/(kg.d)),SHR组皮下注射等体积生理盐水,WKY组不作处理。在第4、8、16和24周龄时间点分别处死动物(每组4只),提取心脏做H.E染色和P-ERK1/2免疫组化染色。结果:SHR组和SHR-T组从8周龄开始血压显著升高,明显高于WKY组(P<0.01),ADM治疗至24周龄时,血压明显低于SHR组;随着大鼠周龄增加,SHR组和SHR-T组大鼠心脏/体重比值逐渐增大。16周龄后,SHR组和SHR-T组的心脏/体重比值大于WKY组(P<0.05),24周龄SHR-T组大鼠的心脏/体重比值小于SHR组(P<0.05);随着大鼠周龄增加,SHR和SHR-T组大鼠相对左心室壁厚度呈增加趋势,24周龄SHR组大鼠相对左心室壁厚度大于同周龄WKY组(P<0.05)。SHR-T组大鼠相对左心室壁厚度在16周龄后增幅放缓,24周龄时小于SHR组(P<0.05);随着大鼠周龄的增加p-ERK1/2在大鼠心肌中的阳性表达逐渐增加,24周龄SHR组大鼠心脏p-ERK1/2的表达率显著高于WKY组(P<0.01),24周龄SHR-T组大鼠心脏p-ERK1/2的表达率显著低于SHR组(P<0.05)。结论:ADM可一定程度减缓自发性高血压大鼠血压的升高和心脏重构,p-ERK1/2的表达量的降低可能参与了ADM发挥其保护作用。
文摘目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞机化后基因表达的差异。方法:40只C57小鼠随机分为对照组(CNC组12只)及癫痫组PTZ (CPTZ组(戊四氮pentylenetetrazole,PTZ)组28只),CNC组给予生理盐水,CPTZ组给予戊四氮,隔日腹腔注射,共28 d。诱导癫痫过程中,观察小鼠行为,癫痫发作级别、持续时间及结局,实验结束后取材,进行相应转录组测序分析。样本的MethylationEPIC BeadChip实验及数据分析由欧易生物技术有限公司(中国上海)进行。结果:转录组相关测序显示癫痫发作改变小鼠脑组织海马区小胶质细胞M2型的基因表达,其中表达上调基因245个,表达下调基因88个。结论:癫痫发作改变小鼠海马区小胶质细胞的基因异常表达。Objective: To investigate the effects of epilepsy on the mechanized gene expression of microglia in hippocampus of C57 mouse. Methods: 40 C57 mice were randomly divided into control group (12 in CNC group) and PTZ group (28 in CPTZ group). The CNC group was given normal saline, and the CPTZ group was given pentatetrazole by intraperitoneal injection every other day for a total of 28 days. During the process of inducing epilepsy, the behavior, seizure level, duration and outcome of the mice were observed. After the experiment, the samples were collected and analyzed by transcriptome sequencing. Sample MethylationEPIC BeadChip experiments and data analysis were performed by OYI Biotechnology Co., Ltd. (Shanghai, China). Results: Transcriptome-related sequencing showed that epileptic seizure changed the expression of M2 type of microglia in the hippocampus of mouse brain tissue, including 245 up-regulated genes and 88 down-regulated genes. Conclusion: Epileptic seizure changes the abnormal gene expression of microglia in hippocampus of mouse.
文摘目的:研究癫痫对C57小鼠脑组织海马区小胶质细胞极化后基因的富集分析。方法:将40只C57小鼠利用随机分组的方法随机分为对照组(CNC组12只)和癫痫组(OPTZ组28只)。CNC组每隔一天腹腔注射生理盐水,OPTZ组每隔一天腹腔注射戊四唑,共28天。在诱导癫痫的实验过程中,观察小鼠的日常行为、癫痫发作程度、癫痫持续时间和癫痫发作后的结局。实验结束后采集样本,进行转录组测序分析。OE生物技术有限公司(上海,中国)负责样品Methylation EPIC Bead Chip实验结果和数据分析的进行。结果:转录组相关测序显示癫痫发作改变小鼠脑组织海马区小胶质细胞M2型的基因表达,其中表达上调基因245个,表达下调基因88个,GO富集分析显示上调基因富集于影响小胶质细胞生长和增殖的通路。结论:癫痫发作改变小鼠海马区小胶质细胞的基因表达且富集于抑制小胶质细胞生长发育通路。Objective: To study the gene enrichment of microglia in hippocampal region of C57 mouse brain after polarization induced by epilepsy. Methods: 40 C57 mice were randomly divided into control group (12 in CNC group) and epilepsy group (28 in OPTZ group). The CNC group was intraperitoneally injected with normal saline every other day, and the OPTZ group was intraperitoneally injected with pentatetrazole every other day for 28 days. In the course of the experiment to induce epilepsy, the daily behavior of the mouse, the degree of seizure, the duration of seizure and the outcome after seizure were observed. After the experiment, the samples were collected and analyzed by transcriptome sequencing. OE Biotechnology Co. Ltd. (Shanghai, China) was responsible for the experimental results and data analysis of the sample, Methylation EPIC Bead Chip. Results: Transcriptome sequencing showed that seizures changed the M2 gene expression of microglia in the hippocampus of mouse brain tissue, including 245 up-regulated genes and 88 down-regulated genes. GO enrichment analysis showed that up-regulated genes were enriched in the pathway affecting the growth and proliferation of microglia. Conclusion: Epileptic seizure changes the gene expression of microglia in the hippocampus of mouse and is concentrated in the pathway that inhibits the growth and development of microglia.
