培养具有创新实践能力和解决实际问题的应用型人才,是当今高等院校人才培养的核心目标。随着信息技术的快速发展,人工智能给教育教学注入了新能量,为更好发挥微生物学实验课程在应用型人才培养中的重要作用,探讨了微生物实验课程新型教...培养具有创新实践能力和解决实际问题的应用型人才,是当今高等院校人才培养的核心目标。随着信息技术的快速发展,人工智能给教育教学注入了新能量,为更好发挥微生物学实验课程在应用型人才培养中的重要作用,探讨了微生物实验课程新型教学思路,通过构建优化实验教学体系,改进实验教学方式,建立开放实验室,完善课程考核制度,打造“学生为主体”课堂;注重过程管理等改革,切实提高了学生学习兴趣和创新实践能力,有效提升教学质量。Cultivating applied talents with innovative practical abilities and problem-solving skills is the core goal of talent cultivation in today’s higher education institutions. With the rapid development of information technology, artificial intelligence has injected new energy into education and teaching. In order to better leverage the important role of microbiology laboratory courses in the cultivation of applied talents, new teaching ideas for environmental microbiology experiment courses were explored. We have constructed and optimized an experimental teaching system, improved experimental teaching methods, established open laboratories, improved course assessment systems, created a “student-centered” classroom and emphasized experimental process management, etc. Through these reforms, students’ interest in learning and innovative practical abilities were effectively improved. The teaching quality has been greatly improved.展开更多
采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。...采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。结果表明,获得1株产β-葡萄糖苷酶能力较强的假丝酵母Candida apicola kj312。该菌株所产的β-葡萄糖苷酶具有较宽的底物特异性,最适底物为对硝基苯基-β-D-吡喃葡萄糖苷;最适反应温度为60℃,在25~65℃范围内,相对酶活力>50%;最适p H值为4.5,在p H值为4.0~7.0酸性范围内,相对酶活力>80%;1 mmol/L的Ca2+、Mn2+、Zn2+和Fe2+及100 mmol/L的Ca2+、Zn2+和Fe2+能显著提高酶活力。展开更多
文摘培养具有创新实践能力和解决实际问题的应用型人才,是当今高等院校人才培养的核心目标。随着信息技术的快速发展,人工智能给教育教学注入了新能量,为更好发挥微生物学实验课程在应用型人才培养中的重要作用,探讨了微生物实验课程新型教学思路,通过构建优化实验教学体系,改进实验教学方式,建立开放实验室,完善课程考核制度,打造“学生为主体”课堂;注重过程管理等改革,切实提高了学生学习兴趣和创新实践能力,有效提升教学质量。Cultivating applied talents with innovative practical abilities and problem-solving skills is the core goal of talent cultivation in today’s higher education institutions. With the rapid development of information technology, artificial intelligence has injected new energy into education and teaching. In order to better leverage the important role of microbiology laboratory courses in the cultivation of applied talents, new teaching ideas for environmental microbiology experiment courses were explored. We have constructed and optimized an experimental teaching system, improved experimental teaching methods, established open laboratories, improved course assessment systems, created a “student-centered” classroom and emphasized experimental process management, etc. Through these reforms, students’ interest in learning and innovative practical abilities were effectively improved. The teaching quality has been greatly improved.
文摘采用七叶苷分离培养基初筛、4-硝基苯-β-D-吡喃葡萄糖苷(p-NPGal)显色法复筛的方法从广东客家娘酒发酵过程中的酒糟中筛选产β-葡萄糖苷酶能力较强的酵母菌,通过26S r DNA D1/D2区基因序列分析对其进行鉴定,并对其酶学性质进行分析。结果表明,获得1株产β-葡萄糖苷酶能力较强的假丝酵母Candida apicola kj312。该菌株所产的β-葡萄糖苷酶具有较宽的底物特异性,最适底物为对硝基苯基-β-D-吡喃葡萄糖苷;最适反应温度为60℃,在25~65℃范围内,相对酶活力>50%;最适p H值为4.5,在p H值为4.0~7.0酸性范围内,相对酶活力>80%;1 mmol/L的Ca2+、Mn2+、Zn2+和Fe2+及100 mmol/L的Ca2+、Zn2+和Fe2+能显著提高酶活力。
