目的:制定护理实习生共情沟通培训课程方案,并评价其实施效果。方法:便利抽取在河南省某三甲医院实习的护理本科生133名为研究对象,对其实施以共情机会–反应模式为框架的共情沟通课程培训,并评价其培训应用效果。结果:培训后,89.5%的...目的:制定护理实习生共情沟通培训课程方案,并评价其实施效果。方法:便利抽取在河南省某三甲医院实习的护理本科生133名为研究对象,对其实施以共情机会–反应模式为框架的共情沟通课程培训,并评价其培训应用效果。结果:培训后,89.5%的实习生对培训课程整体设置满意,92.5%的学生对培训效果和课程收获满意;实习生对共情相关理论培训满意度为78.9%,对情景模拟教学及标准化病人教学满意度分别为91.0%、94.7%。培训前后比较,护理实习生临床沟通能力(t = −7.804, P t = −5.744, P Objective: To develop a training program of empathic communication for nursing interns and evaluate its implementation effect. Methods: A total of 133 undergraduate nursing students were selected from a top three hospital in Henan Province, and the empathic communication course was trained with the framework of empathic opportunity-response model, and the effect of the training was evaluated. Results: After the training, 89.5% of the trainees were satisfied with the training course, and 92.5% of the students were satisfied with the training effect and the course harvest. The trainees’ satisfaction with empathy-related theoretical training was 78.9%, and the trainees’ satisfaction with situational simulation teaching and standardized patient teaching was 91.0% and 94.7%, respectively. Before and after training, there were significant differences in clinical communication ability (t = −7.804, P t = −5.744, P < 0.01). Conclusion: Empathy communication training can improve the clinical communication and empathy ability of nursing interns, and can be applied to the clinical training stage of nursing interns.展开更多
目的:探讨槐定碱对神经胶质瘤U87细胞增殖和侵袭的抑制作用以及U87细胞DNA拓扑异构酶I(DNA TOP I)、EGFR-酪氨酸激酶(EGFR-TK)、基质金属蛋白酶2(MMP2)和氨肽酶N(APN)活性的影响。方法:将不同浓度槐定碱加入到神经胶质瘤U87细胞株中,利...目的:探讨槐定碱对神经胶质瘤U87细胞增殖和侵袭的抑制作用以及U87细胞DNA拓扑异构酶I(DNA TOP I)、EGFR-酪氨酸激酶(EGFR-TK)、基质金属蛋白酶2(MMP2)和氨肽酶N(APN)活性的影响。方法:将不同浓度槐定碱加入到神经胶质瘤U87细胞株中,利用MTT法测定槐定碱对U87细胞和人脑正常星型胶质HEB细胞生长的抑制作用,酶标仪法检测槐定碱对细胞凋亡蛋白caspase-3活性,采用Transwell小室法分析U87细胞的侵袭能力;采用非放射性NF-κB EMSA试剂盒测定U87细胞中NF-κB表达。结果:随着槐定碱浓度的增加(5、10、25、50、100μmol/L),神经胶质瘤U87细胞生长抑制率不断的增加,但HEB细胞的生长并未受到明显的抑制[(11.23±1.18)%vs(2.43±0.29)%、(22.48±3.21)%vs(3.65±0.42)%、(43.21±4.09)%vs(4.03±0.55)%、(57.31±5.09)%vs(5.21±0.43)%、(77.98±6.98)%vs(7.22±0.78)%,均P<0.05];与空白组比较,槐定碱存在下的U87细胞侵袭能力明显降低[(87.43±7.33)%、(65.12±6.16)%、(50.63±4.56)%、(35.32±4.04)%、(23.46±2.32)%vs(120.32±9.32),均P<0.05]。槐定碱对DNA TOP I、EGFR-TK、APN和MMP-2的半抑制率IC_(50)分别为(22.43±2.21)、(31.25±3.09)、(6.32±0.32)和(8.23±0.63)μmol/L,但U87细胞中凋亡蛋白caspase-3活性呈增强趋势;槐定碱能够下调NF-κB信号的表达。结论:低毒性的槐定碱可能通过降低DNA TOP I、EGFR-TK、APN和MMP-2活性,并下调NF-κB信号通路和激活凋亡caspase-3酶联反应的方式,对神经胶质瘤U87细胞的侵袭、增殖和信号通路产生抑制作用。展开更多
文摘目的:制定护理实习生共情沟通培训课程方案,并评价其实施效果。方法:便利抽取在河南省某三甲医院实习的护理本科生133名为研究对象,对其实施以共情机会–反应模式为框架的共情沟通课程培训,并评价其培训应用效果。结果:培训后,89.5%的实习生对培训课程整体设置满意,92.5%的学生对培训效果和课程收获满意;实习生对共情相关理论培训满意度为78.9%,对情景模拟教学及标准化病人教学满意度分别为91.0%、94.7%。培训前后比较,护理实习生临床沟通能力(t = −7.804, P t = −5.744, P Objective: To develop a training program of empathic communication for nursing interns and evaluate its implementation effect. Methods: A total of 133 undergraduate nursing students were selected from a top three hospital in Henan Province, and the empathic communication course was trained with the framework of empathic opportunity-response model, and the effect of the training was evaluated. Results: After the training, 89.5% of the trainees were satisfied with the training course, and 92.5% of the students were satisfied with the training effect and the course harvest. The trainees’ satisfaction with empathy-related theoretical training was 78.9%, and the trainees’ satisfaction with situational simulation teaching and standardized patient teaching was 91.0% and 94.7%, respectively. Before and after training, there were significant differences in clinical communication ability (t = −7.804, P t = −5.744, P < 0.01). Conclusion: Empathy communication training can improve the clinical communication and empathy ability of nursing interns, and can be applied to the clinical training stage of nursing interns.
文摘目的:探讨槐定碱对神经胶质瘤U87细胞增殖和侵袭的抑制作用以及U87细胞DNA拓扑异构酶I(DNA TOP I)、EGFR-酪氨酸激酶(EGFR-TK)、基质金属蛋白酶2(MMP2)和氨肽酶N(APN)活性的影响。方法:将不同浓度槐定碱加入到神经胶质瘤U87细胞株中,利用MTT法测定槐定碱对U87细胞和人脑正常星型胶质HEB细胞生长的抑制作用,酶标仪法检测槐定碱对细胞凋亡蛋白caspase-3活性,采用Transwell小室法分析U87细胞的侵袭能力;采用非放射性NF-κB EMSA试剂盒测定U87细胞中NF-κB表达。结果:随着槐定碱浓度的增加(5、10、25、50、100μmol/L),神经胶质瘤U87细胞生长抑制率不断的增加,但HEB细胞的生长并未受到明显的抑制[(11.23±1.18)%vs(2.43±0.29)%、(22.48±3.21)%vs(3.65±0.42)%、(43.21±4.09)%vs(4.03±0.55)%、(57.31±5.09)%vs(5.21±0.43)%、(77.98±6.98)%vs(7.22±0.78)%,均P<0.05];与空白组比较,槐定碱存在下的U87细胞侵袭能力明显降低[(87.43±7.33)%、(65.12±6.16)%、(50.63±4.56)%、(35.32±4.04)%、(23.46±2.32)%vs(120.32±9.32),均P<0.05]。槐定碱对DNA TOP I、EGFR-TK、APN和MMP-2的半抑制率IC_(50)分别为(22.43±2.21)、(31.25±3.09)、(6.32±0.32)和(8.23±0.63)μmol/L,但U87细胞中凋亡蛋白caspase-3活性呈增强趋势;槐定碱能够下调NF-κB信号的表达。结论:低毒性的槐定碱可能通过降低DNA TOP I、EGFR-TK、APN和MMP-2活性,并下调NF-κB信号通路和激活凋亡caspase-3酶联反应的方式,对神经胶质瘤U87细胞的侵袭、增殖和信号通路产生抑制作用。
