During the past few years, atomic force microscope (AFM) is becoming a powerful tool in life science research. With their high-resolution and capability to image under aqueous solution, AFM is applied to investigate t...During the past few years, atomic force microscope (AFM) is becoming a powerful tool in life science research. With their high-resolution and capability to image under aqueous solution, AFM is applied to investigate the structure of biomolecules and biological process in real time. From visualization of DNA and IgG to DNA transcription, the advances in sample preparation methods have made these events come true. In addition, AFM can be used in molecular recognition. This article illustrates these major achievements of AFM in biological studies.展开更多
微藻生长速度快、CO_(2)固定效率高,每生产1 t微藻生物质可固定183 t CO_(2)。同时,微藻还可将固定的CO_(2)转化为油脂、蛋白质、多糖、色素和不饱和脂肪酸等物质,能够实现CO_(2)的高值化利用。因此,微藻生物固碳技术在CO_(2)捕集和利...微藻生长速度快、CO_(2)固定效率高,每生产1 t微藻生物质可固定183 t CO_(2)。同时,微藻还可将固定的CO_(2)转化为油脂、蛋白质、多糖、色素和不饱和脂肪酸等物质,能够实现CO_(2)的高值化利用。因此,微藻生物固碳技术在CO_(2)捕集和利用方面具有极大的发展潜能。本文首先阐述了高效固定CO_(2)藻株的选育、提高微藻生物固定CO_(2)的培养策略、微藻处理烟道气化合物技术、微藻高效培养光生物反应器的开发及新兴技术助力微藻碳减排等内容,再结合现阶段微藻生物固碳技术所面临的挑战,展望了微藻生物固定CO_(2)在“双碳”目标中的应用前景,以期为利用微藻高效固定CO_(2)、高值化利用CO_(2)提供参考,从而加速“双碳”目标的实现。展开更多
规律间隔成簇短回文重复序列及其相关蛋白9(Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术作为一项基因工程领域革新式的技术,为癌症、遗传性疾病及感染...规律间隔成簇短回文重复序列及其相关蛋白9(Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术作为一项基因工程领域革新式的技术,为癌症、遗传性疾病及感染性疾病等多种重大疾病的治疗提供了极大的帮助.但如何在特定细胞和组织中实现时空调控的精准基因编辑,进而避免脱靶效应,依然是该技术在临床转化领域面临的重要挑战.近年来,通过化学分子和反应实现对CRISPR/Cas9活性的调控已经成为提升这项基因编辑技术效率的重要手段之一.本文综合评述了一些最近报道的化学调控CRISPR/Cas9基因编辑的方法,并对其在临床医学领域的应用前景进行了展望.展开更多
基金国家自然科学基金资助 (No .3 9670 690 ) 广东省自然科学基金资助 (No .960 196)
文摘During the past few years, atomic force microscope (AFM) is becoming a powerful tool in life science research. With their high-resolution and capability to image under aqueous solution, AFM is applied to investigate the structure of biomolecules and biological process in real time. From visualization of DNA and IgG to DNA transcription, the advances in sample preparation methods have made these events come true. In addition, AFM can be used in molecular recognition. This article illustrates these major achievements of AFM in biological studies.
文摘微藻生长速度快、CO_(2)固定效率高,每生产1 t微藻生物质可固定183 t CO_(2)。同时,微藻还可将固定的CO_(2)转化为油脂、蛋白质、多糖、色素和不饱和脂肪酸等物质,能够实现CO_(2)的高值化利用。因此,微藻生物固碳技术在CO_(2)捕集和利用方面具有极大的发展潜能。本文首先阐述了高效固定CO_(2)藻株的选育、提高微藻生物固定CO_(2)的培养策略、微藻处理烟道气化合物技术、微藻高效培养光生物反应器的开发及新兴技术助力微藻碳减排等内容,再结合现阶段微藻生物固碳技术所面临的挑战,展望了微藻生物固定CO_(2)在“双碳”目标中的应用前景,以期为利用微藻高效固定CO_(2)、高值化利用CO_(2)提供参考,从而加速“双碳”目标的实现。
文摘规律间隔成簇短回文重复序列及其相关蛋白9(Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9,CRISPR/Cas9)基因编辑技术作为一项基因工程领域革新式的技术,为癌症、遗传性疾病及感染性疾病等多种重大疾病的治疗提供了极大的帮助.但如何在特定细胞和组织中实现时空调控的精准基因编辑,进而避免脱靶效应,依然是该技术在临床转化领域面临的重要挑战.近年来,通过化学分子和反应实现对CRISPR/Cas9活性的调控已经成为提升这项基因编辑技术效率的重要手段之一.本文综合评述了一些最近报道的化学调控CRISPR/Cas9基因编辑的方法,并对其在临床医学领域的应用前景进行了展望.
