The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis....The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis.Expanding enzyme catalysis to a larger substrate scope and greater variety of chemical reactions and tuning the microenvironment surrounding enzyme molecules to achieve high enzyme performance are urgently needed.In this account,we focus on our efforts using the de novo approach to synthesis hybrid enzyme catalysts that can address these two challenges and the structure-function relationship is discussed to reveal the principles of designing hybrid enzyme catalysts.We hope that this account will promote further efforts toward fundamental research and wide applications of designed enzyme hybrid catalysts for expanding biocatalysis.展开更多
野大豆(Glycine soja Siebold&Zucc.)是一种重要的粮油兼用作物,富含多种黄酮和黄酮糖苷,具有多种药理活性。糖基化可以将黄酮苷元转化为更稳定、生物活性更强、结构更多样的糖苷。通过基因组分析和酶挖掘,报道了一种来自野大豆的黄...野大豆(Glycine soja Siebold&Zucc.)是一种重要的粮油兼用作物,富含多种黄酮和黄酮糖苷,具有多种药理活性。糖基化可以将黄酮苷元转化为更稳定、生物活性更强、结构更多样的糖苷。通过基因组分析和酶挖掘,报道了一种来自野大豆的黄酮UDP-糖基转移酶GsUGT1,该酶主要作用于甘草素7-OH位置,对6种黄酮受体具有催化活性。此外,在大肠杆菌中成功异源表达GsUGT1并对其纯化,酶学性质表征发现在pH为7.0、温度为35℃时,酶的催化活性达到最大,米氏动力学研究表明GsUGT1的催化效率k_(cat)/K_(m)为6.70×10^(-5)μmol/(L·s)。最后通过分子对接对GsUGT1的催化机制进行了初步探究。首次报道了野大豆来源的UDP-糖基转移酶GsUGT1,该酶有望成为酶法合成黄酮糖苷衍生物的有效工具。展开更多
文摘The two major challenges in industrial enzymatic catalysis are the limited number of chemical reaction types that are catalyzed by enzymes and the instability of enzymes under harsh conditions in industrial catalysis.Expanding enzyme catalysis to a larger substrate scope and greater variety of chemical reactions and tuning the microenvironment surrounding enzyme molecules to achieve high enzyme performance are urgently needed.In this account,we focus on our efforts using the de novo approach to synthesis hybrid enzyme catalysts that can address these two challenges and the structure-function relationship is discussed to reveal the principles of designing hybrid enzyme catalysts.We hope that this account will promote further efforts toward fundamental research and wide applications of designed enzyme hybrid catalysts for expanding biocatalysis.
文摘野大豆(Glycine soja Siebold&Zucc.)是一种重要的粮油兼用作物,富含多种黄酮和黄酮糖苷,具有多种药理活性。糖基化可以将黄酮苷元转化为更稳定、生物活性更强、结构更多样的糖苷。通过基因组分析和酶挖掘,报道了一种来自野大豆的黄酮UDP-糖基转移酶GsUGT1,该酶主要作用于甘草素7-OH位置,对6种黄酮受体具有催化活性。此外,在大肠杆菌中成功异源表达GsUGT1并对其纯化,酶学性质表征发现在pH为7.0、温度为35℃时,酶的催化活性达到最大,米氏动力学研究表明GsUGT1的催化效率k_(cat)/K_(m)为6.70×10^(-5)μmol/(L·s)。最后通过分子对接对GsUGT1的催化机制进行了初步探究。首次报道了野大豆来源的UDP-糖基转移酶GsUGT1,该酶有望成为酶法合成黄酮糖苷衍生物的有效工具。
