目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UV...目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UVA和H2O2分别处理细胞使其衰老,建立早衰模型。此后进行CPE的慢病毒转染,获得CPE稳定敲除的细胞株。通过qRT-PCR和WB技术,检测CPE和衰老标志物蛋白及mRNA的表达。分别采用cck8和SA-β-gal染色检测CPE敲降后NHSFs的增殖和衰老程度。最后采用qRT-PCR检测CPE敲降后NHSFs促炎症因子的表达水平。结果:N1组CPE蛋白和mRNA表达最高,其次是N2组,N3组最少,早衰细胞模型中CPE蛋白和mRNA的表达减低,CPE敲降后NHSFs蛋白和mRNA的表达均升高、细胞活性降低、细胞衰老程度明显加快和促炎症因子mRNA表达升高。结论:CPE可作为潜在衰老标志物且其高表达可延缓衰老。Objective: To explore the correlation between the expression of CPE and cellular senescence. Methods: Human foreskin fibroblasts (NHSF cells) isolated from healthy human donors (5~20 years old) who underwent surgical defibrillation were selected for passage. Here, we defined “NHSFs of less than 15 generations as group N1, NHSFs of 15~25 generations as group N2, and NHSF of more than 45 generations as group N3”. Then the cells were treated with UVA and H2O2 respectively to make them senescent, and the premature aging model was established. Then lentiviral transfection of CPE was performed to obtain stable CPE knockout cell lines. The expression of CPE and aging marker protein and mRNA were detected by qRT-PCR and WB. The proliferation and senescence of NHSFs after CPE knockdown were detected by cck8 and SA-β-gal staining, respectively. Finally, the expression level of NHSFs proinflammatory factors after CPE knock-down was detected by qRT-PCR. Results: The expressions of CPE protein and mRNA were the highest in N1 group, followed by N2 group and N3 group, and the expressions of CPE protein and mRNA were decreased in the progeria cell model. After CPE knockdown, the expressions of NHSFs protein and mRNA were both increased, the cell activity was decreased, the degree of cell senility was significantly accelerated, and the mRNA expression of proinflammatory factors was increased. Conclusion: CPE can be used as a potential senescence marker and its high expression can delay senescence.展开更多
目的:探讨与退变性腰椎疾病患者经后路腰椎融合手术后发生心脏不良事件可能相关的因素。方法:回顾性分析2018年1月~2023年7月因腰椎退变性疾病在北京安贞医院接受后路腰椎融合手术的96例患者临床资料,其中男性53例,女性44例,平均年龄64....目的:探讨与退变性腰椎疾病患者经后路腰椎融合手术后发生心脏不良事件可能相关的因素。方法:回顾性分析2018年1月~2023年7月因腰椎退变性疾病在北京安贞医院接受后路腰椎融合手术的96例患者临床资料,其中男性53例,女性44例,平均年龄64.1±10.1岁。依据腰椎融合术后1年内是否出现心脏不良事件(心脏骤停、新发或是加重心律失常、急性心肌梗死或是心绞痛及心力衰竭)进行分组,比较两组患者一般情况[查理森共病指数(Charlson comorbidity index,CCI)、美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级、改良心脏危险指数(revised cardiac risk index,RCRI)等]、术前检查(血红蛋白、血浆白蛋白、心脏超声射血分数等)、手术参数(手术有创性Mirza分级、手术时间、术中估计失血量等)以及术中监测指标(基线平均动脉压、术中尿量、自体血回输量等)的差异。结果:腰椎融合术后未发生心脏不良事件组患者CCI、RCRI、超声心动E波最大流速、术中估计失血量、基线平均动脉压以及术中自体血回输均明显小于发生心脏不良事件组(0.9±1.0 vs 1.6±1.5;1.5±0.7 vs 2.0±0.8;70.3±18.2 vs 82.6±36.9;705±560.6 vs 1193±1332.9;103.6±15.9 vs 112.1±12.1;399.5±368.3 vs 637.6±470.5),差异均有统计学意义(P<0.05)。多因素回归分析提示CCI和术中估计失血量与术后发生心脏不良事件存在统计学相关性(OR=1.968,P=0.007;OR=1.001,P=0.023)。结论:退变性腰椎疾病患者经后路腰椎融合术后发生心脏不良事件可能与患者查理森共病指数及手术失血量密切相关。展开更多
目的:主动脉弓离断(interruption of aortic arch,IAA)是一种罕见的先天性心脏疾病。本研究探讨IAA胎儿的超声心动图和病理学超微结构特征,并进一步分析其演变的病理过程。方法:回顾性分析产前诊断并经手术或尸体解剖确诊的IAA胎儿的产...目的:主动脉弓离断(interruption of aortic arch,IAA)是一种罕见的先天性心脏疾病。本研究探讨IAA胎儿的超声心动图和病理学超微结构特征,并进一步分析其演变的病理过程。方法:回顾性分析产前诊断并经手术或尸体解剖确诊的IAA胎儿的产前超声心动图、术后病理检查或尸体解剖结果等资料。采用产前超声心动图追踪观察IAA胎儿主动脉弓各节段内径及Z值,以及狭窄段主动脉弓的变化情况,结合尸体解剖及病理检查结果,探讨胎儿IAA可能的宫内演变过程及其发生的细胞学基础。结果:纳入34例IAA胎儿,分别有3、3、28例产前超声诊断为主动脉弓发育不良(aortic arch dysplasia,AAD)、主动脉缩窄(coarctation of aorta,CoA)、IAA。3例AAD和3例CoA胎儿均在产前超声检查1~2周后选择引产,并经尸体解剖证实为IAA。28例产前超声诊断为IAA的胎儿中6例为CoA进行性加重,在超声心动图追踪复查的过程中发现逐渐演变为A型IAA;其他22例首次产前超声即诊断为IAA。3例胎儿分娩后经手术矫正,27例选择终止妊娠,4例宫内死亡。胎儿IAA的超声心动图主要表现包括:心脏四腔心切面左心室明显小于右心室或差别不明显;三血管切面显示主动脉明显小于肺动脉;三血管-气管切面和主动脉弓切面显示主动脉与降主动脉不能相连;主动脉弓切面显示主动脉弓弯曲度变小、走行僵直,主动脉、动脉导管和降主动脉之间失去正常的“V”字形结构。彩色多普勒超声检查显示主动脉弓中断处无连续血流信号,动脉导管内可见血流反转。对7例IAA胎儿进行透射电镜检测,结果显示主动脉弓近离断处的弹力膜之间存在大量平滑肌细胞,数量较升主动脉近端明显增多且排列紊乱,弹力膜较升主动脉近端增厚、走行扭曲;离断处系带无明显正常的内皮细胞和管腔,只有少量断裂坏死内皮细胞的痕迹,大量粗短且排列紊乱的弹力膜和纵横交错的平滑肌细胞无规律分布。结论:产前超声心动图是诊断胎儿IAA的主要手段,通过术后追踪和尸体解剖发现胎儿IAA的并发症和疾病特征,有利于提高其诊断率;部分胎儿IAA可由AAD或CoA逐步演变而来,其发病机制可能与缺血缺氧和动脉导管收缩成分迁移有关。展开更多
文摘目的:探讨CPE的表达与细胞衰老的相关性。方法:选取接受手术清创的健康人供体(5~20岁)分离的人包皮的成纤维细胞(NHSF细胞)将其进行传代,在此我们定义“小于15代的NHSFs为N1组、15~25代的NHSFs为N2组、大于45代的NHSF为N3组”。随后用UVA和H2O2分别处理细胞使其衰老,建立早衰模型。此后进行CPE的慢病毒转染,获得CPE稳定敲除的细胞株。通过qRT-PCR和WB技术,检测CPE和衰老标志物蛋白及mRNA的表达。分别采用cck8和SA-β-gal染色检测CPE敲降后NHSFs的增殖和衰老程度。最后采用qRT-PCR检测CPE敲降后NHSFs促炎症因子的表达水平。结果:N1组CPE蛋白和mRNA表达最高,其次是N2组,N3组最少,早衰细胞模型中CPE蛋白和mRNA的表达减低,CPE敲降后NHSFs蛋白和mRNA的表达均升高、细胞活性降低、细胞衰老程度明显加快和促炎症因子mRNA表达升高。结论:CPE可作为潜在衰老标志物且其高表达可延缓衰老。Objective: To explore the correlation between the expression of CPE and cellular senescence. Methods: Human foreskin fibroblasts (NHSF cells) isolated from healthy human donors (5~20 years old) who underwent surgical defibrillation were selected for passage. Here, we defined “NHSFs of less than 15 generations as group N1, NHSFs of 15~25 generations as group N2, and NHSF of more than 45 generations as group N3”. Then the cells were treated with UVA and H2O2 respectively to make them senescent, and the premature aging model was established. Then lentiviral transfection of CPE was performed to obtain stable CPE knockout cell lines. The expression of CPE and aging marker protein and mRNA were detected by qRT-PCR and WB. The proliferation and senescence of NHSFs after CPE knockdown were detected by cck8 and SA-β-gal staining, respectively. Finally, the expression level of NHSFs proinflammatory factors after CPE knock-down was detected by qRT-PCR. Results: The expressions of CPE protein and mRNA were the highest in N1 group, followed by N2 group and N3 group, and the expressions of CPE protein and mRNA were decreased in the progeria cell model. After CPE knockdown, the expressions of NHSFs protein and mRNA were both increased, the cell activity was decreased, the degree of cell senility was significantly accelerated, and the mRNA expression of proinflammatory factors was increased. Conclusion: CPE can be used as a potential senescence marker and its high expression can delay senescence.
文摘目的:探讨与退变性腰椎疾病患者经后路腰椎融合手术后发生心脏不良事件可能相关的因素。方法:回顾性分析2018年1月~2023年7月因腰椎退变性疾病在北京安贞医院接受后路腰椎融合手术的96例患者临床资料,其中男性53例,女性44例,平均年龄64.1±10.1岁。依据腰椎融合术后1年内是否出现心脏不良事件(心脏骤停、新发或是加重心律失常、急性心肌梗死或是心绞痛及心力衰竭)进行分组,比较两组患者一般情况[查理森共病指数(Charlson comorbidity index,CCI)、美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级、改良心脏危险指数(revised cardiac risk index,RCRI)等]、术前检查(血红蛋白、血浆白蛋白、心脏超声射血分数等)、手术参数(手术有创性Mirza分级、手术时间、术中估计失血量等)以及术中监测指标(基线平均动脉压、术中尿量、自体血回输量等)的差异。结果:腰椎融合术后未发生心脏不良事件组患者CCI、RCRI、超声心动E波最大流速、术中估计失血量、基线平均动脉压以及术中自体血回输均明显小于发生心脏不良事件组(0.9±1.0 vs 1.6±1.5;1.5±0.7 vs 2.0±0.8;70.3±18.2 vs 82.6±36.9;705±560.6 vs 1193±1332.9;103.6±15.9 vs 112.1±12.1;399.5±368.3 vs 637.6±470.5),差异均有统计学意义(P<0.05)。多因素回归分析提示CCI和术中估计失血量与术后发生心脏不良事件存在统计学相关性(OR=1.968,P=0.007;OR=1.001,P=0.023)。结论:退变性腰椎疾病患者经后路腰椎融合术后发生心脏不良事件可能与患者查理森共病指数及手术失血量密切相关。
文摘目的:主动脉弓离断(interruption of aortic arch,IAA)是一种罕见的先天性心脏疾病。本研究探讨IAA胎儿的超声心动图和病理学超微结构特征,并进一步分析其演变的病理过程。方法:回顾性分析产前诊断并经手术或尸体解剖确诊的IAA胎儿的产前超声心动图、术后病理检查或尸体解剖结果等资料。采用产前超声心动图追踪观察IAA胎儿主动脉弓各节段内径及Z值,以及狭窄段主动脉弓的变化情况,结合尸体解剖及病理检查结果,探讨胎儿IAA可能的宫内演变过程及其发生的细胞学基础。结果:纳入34例IAA胎儿,分别有3、3、28例产前超声诊断为主动脉弓发育不良(aortic arch dysplasia,AAD)、主动脉缩窄(coarctation of aorta,CoA)、IAA。3例AAD和3例CoA胎儿均在产前超声检查1~2周后选择引产,并经尸体解剖证实为IAA。28例产前超声诊断为IAA的胎儿中6例为CoA进行性加重,在超声心动图追踪复查的过程中发现逐渐演变为A型IAA;其他22例首次产前超声即诊断为IAA。3例胎儿分娩后经手术矫正,27例选择终止妊娠,4例宫内死亡。胎儿IAA的超声心动图主要表现包括:心脏四腔心切面左心室明显小于右心室或差别不明显;三血管切面显示主动脉明显小于肺动脉;三血管-气管切面和主动脉弓切面显示主动脉与降主动脉不能相连;主动脉弓切面显示主动脉弓弯曲度变小、走行僵直,主动脉、动脉导管和降主动脉之间失去正常的“V”字形结构。彩色多普勒超声检查显示主动脉弓中断处无连续血流信号,动脉导管内可见血流反转。对7例IAA胎儿进行透射电镜检测,结果显示主动脉弓近离断处的弹力膜之间存在大量平滑肌细胞,数量较升主动脉近端明显增多且排列紊乱,弹力膜较升主动脉近端增厚、走行扭曲;离断处系带无明显正常的内皮细胞和管腔,只有少量断裂坏死内皮细胞的痕迹,大量粗短且排列紊乱的弹力膜和纵横交错的平滑肌细胞无规律分布。结论:产前超声心动图是诊断胎儿IAA的主要手段,通过术后追踪和尸体解剖发现胎儿IAA的并发症和疾病特征,有利于提高其诊断率;部分胎儿IAA可由AAD或CoA逐步演变而来,其发病机制可能与缺血缺氧和动脉导管收缩成分迁移有关。
