[Objective] An HPLC method for the determination of caffeoylquinic acid compounds in sweet potato was developed in the present research. Quantitative analysis was performed on 11 sweet potato genotypes to provide a re...[Objective] An HPLC method for the determination of caffeoylquinic acid compounds in sweet potato was developed in the present research. Quantitative analysis was performed on 11 sweet potato genotypes to provide a reference for the development and utilization of sweet potato, especial y on the aboveground part. [Method] Caffeoylquinic acid compounds were extracted from sweet potato leaves by ultrasonic treatment for 20 min with 200 volumes of methanol. The mobile phase of HPLC was acetonitrile -0.2% aqueous phosphoric acid. Chlorogenic acid, 4, 5-O-coffeocylquinic acid, 3, 5-O-coffeeacylquinic acid and 3, 4-O-coffeeacylquinic acid were separated wel by gradient elution, with recovery rate of 94.74%, 102.63%, 100.01% and 101.05%, respectively. [Result] The contents of caffeoylquinic acids were significantly different between sweet potato genotypes and tissue parts. Among these compounds, the content of bi-caffeoylquinic acids was the highest in the aboveground part, and the content of chlorogenic acid was the highest in the root tubers. The content of caffeoylquinic acid in different parts of the plant was： 9.77-40.86 mg/g in stem apex, 2.68-13.97 mg/g in mature leaves, 0.56-7.90 mg/g in stems and 0.69-4.33 mg/g in root tubers, respectively. [Conclusion] Sweet potato stems and leaves can be used for the extraction of chlorogenic acid in development and utilization, in which the purple sweet potato series is superior to the non-purple sweet potato series. The performance of the two purple sweet potato materials of 11ZY series is particularly prominent.展开更多
基金Supported by Postdoctoral Sustentation Fund of Hunan Province(2014RS4010)the Open Fund for Key Horticulture Discipline Research of Hunan Agricultural University(2013YYX021)~~
文摘[Objective] An HPLC method for the determination of caffeoylquinic acid compounds in sweet potato was developed in the present research. Quantitative analysis was performed on 11 sweet potato genotypes to provide a reference for the development and utilization of sweet potato, especial y on the aboveground part. [Method] Caffeoylquinic acid compounds were extracted from sweet potato leaves by ultrasonic treatment for 20 min with 200 volumes of methanol. The mobile phase of HPLC was acetonitrile -0.2% aqueous phosphoric acid. Chlorogenic acid, 4, 5-O-coffeocylquinic acid, 3, 5-O-coffeeacylquinic acid and 3, 4-O-coffeeacylquinic acid were separated wel by gradient elution, with recovery rate of 94.74%, 102.63%, 100.01% and 101.05%, respectively. [Result] The contents of caffeoylquinic acids were significantly different between sweet potato genotypes and tissue parts. Among these compounds, the content of bi-caffeoylquinic acids was the highest in the aboveground part, and the content of chlorogenic acid was the highest in the root tubers. The content of caffeoylquinic acid in different parts of the plant was： 9.77-40.86 mg/g in stem apex, 2.68-13.97 mg/g in mature leaves, 0.56-7.90 mg/g in stems and 0.69-4.33 mg/g in root tubers, respectively. [Conclusion] Sweet potato stems and leaves can be used for the extraction of chlorogenic acid in development and utilization, in which the purple sweet potato series is superior to the non-purple sweet potato series. The performance of the two purple sweet potato materials of 11ZY series is particularly prominent.
