To investigate the neuroprotective action of astragalosideⅣ(AS-Ⅳ)on spatial learning and memory impairment induced by amyloid-beta 1-42(Aβ1-42)in rats and elucidate its underlying molecular mechanisms.Methods Adult...To investigate the neuroprotective action of astragalosideⅣ(AS-Ⅳ)on spatial learning and memory impairment induced by amyloid-beta 1-42(Aβ1-42)in rats and elucidate its underlying molecular mechanisms.Methods Adult-male Sprague-Dawley rats(230-250 g)were divided into six groups randomly:control,Aβ1-42,AS-Ⅳ,Aβ1-42 plus 5 mg/kg·d AS-Ⅳ,Aβ1-42 plus 25 mg/kg·d AS-Ⅳ,and Aβ1-42 plus 50 mg/kg·d AS-Ⅳgroups.Aβ1-42 were delivered by intracerebroventricular injection under the guidance of a brain stereotaxic apparatus.The Morris water maze test(hidden platform test,probe trials,visible platform test)was performed one week after Aβ1-42 injection to obtain the ability of rat spatial learning and memory.AS-Ⅳ(5,25 and 50 mg/kg·d)was administrated intraperitoneally once per day from the 8th day after Aβ1-42 injection for 5 consecutive days.Average escape latencies,distances for searching for the platform under water and the percentage of total time elapsed and distance swam in the right quadrant after removing platform were determined by behavior software system.展开更多
基金Supported by the Natural Science Foundation of Shaanxi Province of China(2015JQ8299)National Science Foundation of China(81703842)Traditional Chinese Medicine Scientific Research Projects of Shaanxi Province of China(JCMS032)
文摘To investigate the neuroprotective action of astragalosideⅣ(AS-Ⅳ)on spatial learning and memory impairment induced by amyloid-beta 1-42(Aβ1-42)in rats and elucidate its underlying molecular mechanisms.Methods Adult-male Sprague-Dawley rats(230-250 g)were divided into six groups randomly:control,Aβ1-42,AS-Ⅳ,Aβ1-42 plus 5 mg/kg·d AS-Ⅳ,Aβ1-42 plus 25 mg/kg·d AS-Ⅳ,and Aβ1-42 plus 50 mg/kg·d AS-Ⅳgroups.Aβ1-42 were delivered by intracerebroventricular injection under the guidance of a brain stereotaxic apparatus.The Morris water maze test(hidden platform test,probe trials,visible platform test)was performed one week after Aβ1-42 injection to obtain the ability of rat spatial learning and memory.AS-Ⅳ(5,25 and 50 mg/kg·d)was administrated intraperitoneally once per day from the 8th day after Aβ1-42 injection for 5 consecutive days.Average escape latencies,distances for searching for the platform under water and the percentage of total time elapsed and distance swam in the right quadrant after removing platform were determined by behavior software system.
