This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guang...This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guangxiensis Yang Yang, 1988 and Holorusia basiflava Yang Yang, 1993 and female of Pselliophora xanthopimplina Enderlein, 1921 are redescribed and illustrated with new morphological characters. The females of P. guangxiensis and H. basiflava are described and illustrated for the first time. A key for separating known species of Indotipula Edwards, 1931 from China is provided. DNA barcodes of all species in this study are provided and analyzed.展开更多
目的筛选并鉴定软腐白菜根系土壤中分离出的细菌。方法从河南省大白菜田成熟白菜病株根系土壤采集样品,利用模拟原环境的培养基成分和条件,对样品中的细菌进行分离和培养。对菌株进行形态学观察、生理生化特征鉴定以及16S r RNA基因序...目的筛选并鉴定软腐白菜根系土壤中分离出的细菌。方法从河南省大白菜田成熟白菜病株根系土壤采集样品,利用模拟原环境的培养基成分和条件,对样品中的细菌进行分离和培养。对菌株进行形态学观察、生理生化特征鉴定以及16S r RNA基因序列测定、比对分析和系统发育树构建,对菌株进行初步分类鉴定。结果分离到的一株编号为Q2S2-18A2的菌株16Sr DNA与代夫特菌属内的Delftia lacustris 332 T(EU888303)具有最高的相似性,相似值为98.60%。结论模拟原环境培养基,结合经典分类学方法和16S r RNA基因序列比对分析、构建系统发育树,可分离并鉴定软腐白菜根系土壤中的机会致病菌湖生代夫特菌。展开更多
基金supported by the National Natural Science Foundation of China(31300551)the Anhui Outstanding Young Talent Support Program(gxfx2017059)
文摘This paper reports six species of crane flies from Dayaoshan National Nature Reserve, Guangxi Zhuang Autonomous Region, China, including one new species, Indotipula jinxiuensis sp. nov. The males of Pselliophora guangxiensis Yang Yang, 1988 and Holorusia basiflava Yang Yang, 1993 and female of Pselliophora xanthopimplina Enderlein, 1921 are redescribed and illustrated with new morphological characters. The females of P. guangxiensis and H. basiflava are described and illustrated for the first time. A key for separating known species of Indotipula Edwards, 1931 from China is provided. DNA barcodes of all species in this study are provided and analyzed.
文摘目的筛选并鉴定软腐白菜根系土壤中分离出的细菌。方法从河南省大白菜田成熟白菜病株根系土壤采集样品,利用模拟原环境的培养基成分和条件,对样品中的细菌进行分离和培养。对菌株进行形态学观察、生理生化特征鉴定以及16S r RNA基因序列测定、比对分析和系统发育树构建,对菌株进行初步分类鉴定。结果分离到的一株编号为Q2S2-18A2的菌株16Sr DNA与代夫特菌属内的Delftia lacustris 332 T(EU888303)具有最高的相似性,相似值为98.60%。结论模拟原环境培养基,结合经典分类学方法和16S r RNA基因序列比对分析、构建系统发育树,可分离并鉴定软腐白菜根系土壤中的机会致病菌湖生代夫特菌。
