宽频声导抗(Wideband Acoustic Immittance, WAI)是一种新兴的、非侵入性的通过测量中耳对声波的吸收和反射来评估中耳功能的听力检测技术,其覆盖226~8000 Hz频率范围。相比于传统的226 Hz声导抗检查,WAI能提供中耳更全面、精准的信息。...宽频声导抗(Wideband Acoustic Immittance, WAI)是一种新兴的、非侵入性的通过测量中耳对声波的吸收和反射来评估中耳功能的听力检测技术,其覆盖226~8000 Hz频率范围。相比于传统的226 Hz声导抗检查,WAI能提供中耳更全面、精准的信息。WAI在诊断中耳炎、耳硬化症等疾病中显示出了高敏感性和高特异性,同时WAI测试结果仍受到多种因素的影响。本文对宽频声导抗在耳科疾病中的应用进展进行了综述。Wideband Acoustic Immittance (WAI) is an emerging, non-invasive hearing test technology that assesses middle ear function by measuring the absorption and reflection of sound waves in the middle ear, covering a frequency range of 226~8000 Hz. Compared to traditional 226 Hz tympanometry, WAI provides more comprehensive and accurate information about middle ear. High sensitivity and specificity of WAI have been shown in diagnosing diseases such as otitis media and otosclerosis. However, WAI test results may be influenced by various factors. This article is a review of the progress in the application of wideband acoustic immittance in diagnosis of ear diseases.展开更多
目的观察鼻息肉患者磷酸化信号转导与转录激活因子3(phospho-signal transducers and activators of transcription,p-STAT3)、SOCS3(suppressor of cytokine signaling,SOCS3)及Th17相关因子的水平,初步探讨STAT3及SOCS3对鼻息肉患者T...目的观察鼻息肉患者磷酸化信号转导与转录激活因子3(phospho-signal transducers and activators of transcription,p-STAT3)、SOCS3(suppressor of cytokine signaling,SOCS3)及Th17相关因子的水平,初步探讨STAT3及SOCS3对鼻息肉患者Th17细胞功能的作用。方法选取40例鼻息肉患者标本及15例对照组标本,采用免疫组化和蛋白免疫印迹法检测p-STAT3及SOCS3在鼻息肉组织中的分布及表达,运用ELISA法检测IL-6和IL-17A的表达情况,采用RT-PCR检测RORγt的表达水平,并对数据进行统计学分析。结果鼻息肉组的p-STAT3、IL-6、IL-17A以及RORγt表达明显高于对照组(P<0.01),SOCS3在鼻息肉组中的表达则低于对照组(P<0.01)。相关性分析显示p-STAT3、RORγt及IL-17A与SOCS3呈负相关(p-STAT3/SOCS3:r=-0.545,P<0.01;RORγt/SOCS3:r=-0.627,P<0.05;IL-17A/SOCS3:r=-0.631,P<0.01),而p-STAT3与RORγt、IL-6、IL-17A呈正相关(p-STAT3/RORγt:r=0.594,P<0.001;p-STAT3/IL-6:r=0.632,P<0.001;p-STAT3/IL-17A:r=0.647,P<0.01)。结论 STAT3异常活化,以及SOCS3低表达所致抑制作用缺失可能是造成鼻息肉中Th17细胞过度活化的重要原因。展开更多
文摘宽频声导抗(Wideband Acoustic Immittance, WAI)是一种新兴的、非侵入性的通过测量中耳对声波的吸收和反射来评估中耳功能的听力检测技术,其覆盖226~8000 Hz频率范围。相比于传统的226 Hz声导抗检查,WAI能提供中耳更全面、精准的信息。WAI在诊断中耳炎、耳硬化症等疾病中显示出了高敏感性和高特异性,同时WAI测试结果仍受到多种因素的影响。本文对宽频声导抗在耳科疾病中的应用进展进行了综述。Wideband Acoustic Immittance (WAI) is an emerging, non-invasive hearing test technology that assesses middle ear function by measuring the absorption and reflection of sound waves in the middle ear, covering a frequency range of 226~8000 Hz. Compared to traditional 226 Hz tympanometry, WAI provides more comprehensive and accurate information about middle ear. High sensitivity and specificity of WAI have been shown in diagnosing diseases such as otitis media and otosclerosis. However, WAI test results may be influenced by various factors. This article is a review of the progress in the application of wideband acoustic immittance in diagnosis of ear diseases.
文摘目的观察鼻息肉患者磷酸化信号转导与转录激活因子3(phospho-signal transducers and activators of transcription,p-STAT3)、SOCS3(suppressor of cytokine signaling,SOCS3)及Th17相关因子的水平,初步探讨STAT3及SOCS3对鼻息肉患者Th17细胞功能的作用。方法选取40例鼻息肉患者标本及15例对照组标本,采用免疫组化和蛋白免疫印迹法检测p-STAT3及SOCS3在鼻息肉组织中的分布及表达,运用ELISA法检测IL-6和IL-17A的表达情况,采用RT-PCR检测RORγt的表达水平,并对数据进行统计学分析。结果鼻息肉组的p-STAT3、IL-6、IL-17A以及RORγt表达明显高于对照组(P<0.01),SOCS3在鼻息肉组中的表达则低于对照组(P<0.01)。相关性分析显示p-STAT3、RORγt及IL-17A与SOCS3呈负相关(p-STAT3/SOCS3:r=-0.545,P<0.01;RORγt/SOCS3:r=-0.627,P<0.05;IL-17A/SOCS3:r=-0.631,P<0.01),而p-STAT3与RORγt、IL-6、IL-17A呈正相关(p-STAT3/RORγt:r=0.594,P<0.001;p-STAT3/IL-6:r=0.632,P<0.001;p-STAT3/IL-17A:r=0.647,P<0.01)。结论 STAT3异常活化,以及SOCS3低表达所致抑制作用缺失可能是造成鼻息肉中Th17细胞过度活化的重要原因。
