铜死亡是一种特殊的细胞死亡形式。膀胱癌,特别是膀胱尿路上皮癌(BLCA),是全球十大最常见癌症之一。迄今为止,铜死亡在BLCA中的潜在作用尚不明确。在本研究中,我们基于从癌症基因组图谱(TCGA)和基因表达综合(GEO)数据库下载的数据,系统...铜死亡是一种特殊的细胞死亡形式。膀胱癌,特别是膀胱尿路上皮癌(BLCA),是全球十大最常见癌症之一。迄今为止,铜死亡在BLCA中的潜在作用尚不明确。在本研究中,我们基于从癌症基因组图谱(TCGA)和基因表达综合(GEO)数据库下载的数据,系统评估了509个BLCA样本中19个经过验证的与铜中毒相关基因(CRGs)介导的铜中毒模式。使用Kaplan-Meier方法分析不同风险组的总体生存率(OS)。使用基因集变异分析(GSVA)研究不同铜死亡簇(CR簇)之间的差异。使用单样本基因集富集分析(ssGSEA)讨CRG簇与免疫状态之间的潜在关系。我们使用GO (基因本体)和KEGG (京都基因与基因组百科全书)富集分析研究各种细胞生化过程。最后,我们建立了一个预后模型,以预测患者的生存结果,并进一步分析BLCA患者的预测特征与各种治疗反应之间的相关性。在本研究中,我们得出了两个CRG簇和基因簇,并建立了一个模型来量化个体BLCA患者的风险评分,发现其与多种临床特征密切相关,并能够准确预测BLCA患者的预后。我们相信,通过本研究,对单个样本中铜死亡介导模式的定量分析可能有助于提高我们对BLCA多组学特征的理解,并指导未来的治疗方案。Cuproptosis is a special form of cell death. Bladder cancer, especially Bladder Urothelial Carcinoma (BLCA), is one of the ten most common cancer types in the world. So far, the potential role of cuproptosis in BLCA is unclear. In the present study, we systematically evaluated the copper poisoning-mediated patterns of 509 BLCA samples based on 19 validated copper poisoning-related genes (CRGs) using data downloaded from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database. Kaplan Meier method was used to analyze the overall survival rate (OS) of different risk groups. Gene Set Variation Analysis (GSVA) was used to study the functional differences between different cuproptosis clusters (CRG clusters). Single sample gene set enrichment analysis (ssGSEA) was used to explore the potential relationship between CRG clusters and immune status. We used GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis to study various cellular biochemical processes. Finally, we established a prognostic model to predict patients’ survival outcomes and to further analyze the correlation between the predictive characteristics of BLCA patients and various treatment responses. In this study, we derived two CRG clusters and gene clusters and also established a model to quantify the risk score of individual BLCA patients, which was found to be closely associated with various clinical characteristics and could precisely predict the prognosis of BLCA patients. We believe that through our study, quantitative analysis of cuproptosis-mediated patterns in a single sample may help to improve our understanding of the multi-omics characteristics of BLCA and guide future treatment regimens.展开更多
上尿路结石相关性尿脓毒血症是一种危及生命的泌尿系统急症,尤以老年患者及伴有基础疾病者为高危人群。近年来,随着结石处理技术的进步,其发生率和预后有所变化。本文系统综述了该疾病的发病机制、诊断方法及治疗策略,强调早期诊断和及...上尿路结石相关性尿脓毒血症是一种危及生命的泌尿系统急症,尤以老年患者及伴有基础疾病者为高危人群。近年来,随着结石处理技术的进步,其发生率和预后有所变化。本文系统综述了该疾病的发病机制、诊断方法及治疗策略,强调早期诊断和及时干预的重要性,并探讨不同治疗手段对患者结局的影响。在现有研究的基础上,提出优化治疗方案的建议,以期为临床诊疗提供理论依据与实践指导。Upper urinary tract stone-associated urosepsis is a life-threatening urological emergency, particularly prevalent among elderly patients and those with underlying comorbidities. In recent years, advancements in stone management techniques have led to changes in its incidence and prognosis. This review systematically summarizes the pathogenesis, diagnostic methods, and therapeutic strategies of this condition, emphasizing the importance of early diagnosis and timely intervention. Furthermore, it explores the impact of various treatment modalities on patient outcomes. Based on existing studies, recommendations are proposed to optimize treatment protocols, aiming to provide theoretical support and practical guidance for clinical practice.展开更多
上尿路结石相关自发性肾破裂是泌尿外科少见的危急重症,临床表现缺乏特异性,且目前无明确的诊疗规范,临床医生常因为病因判断错误而影响后续治疗。故本文围绕该病的发病机制、临床特征和诊疗现状做一综述,以期为该病的诊疗和研究提供参...上尿路结石相关自发性肾破裂是泌尿外科少见的危急重症,临床表现缺乏特异性,且目前无明确的诊疗规范,临床医生常因为病因判断错误而影响后续治疗。故本文围绕该病的发病机制、临床特征和诊疗现状做一综述,以期为该病的诊疗和研究提供参考。Spontaneous renal rupture linked to upper urinary tract calculi is a rare but critical emergency in the field of urology. The non-specific nature of its clinical manifestations, coupled with the lack of standardized diagnostic and treatment guidelines, frequently leads to misdiagnosis and suboptimal treatment outcomes. This detailed review delves into the pathophysiology, clinical features, and existing diagnostic and treatment strategies for this condition, aiming to enhance clinical practice and inform future research directions.展开更多
文摘铜死亡是一种特殊的细胞死亡形式。膀胱癌,特别是膀胱尿路上皮癌(BLCA),是全球十大最常见癌症之一。迄今为止,铜死亡在BLCA中的潜在作用尚不明确。在本研究中,我们基于从癌症基因组图谱(TCGA)和基因表达综合(GEO)数据库下载的数据,系统评估了509个BLCA样本中19个经过验证的与铜中毒相关基因(CRGs)介导的铜中毒模式。使用Kaplan-Meier方法分析不同风险组的总体生存率(OS)。使用基因集变异分析(GSVA)研究不同铜死亡簇(CR簇)之间的差异。使用单样本基因集富集分析(ssGSEA)讨CRG簇与免疫状态之间的潜在关系。我们使用GO (基因本体)和KEGG (京都基因与基因组百科全书)富集分析研究各种细胞生化过程。最后,我们建立了一个预后模型,以预测患者的生存结果,并进一步分析BLCA患者的预测特征与各种治疗反应之间的相关性。在本研究中,我们得出了两个CRG簇和基因簇,并建立了一个模型来量化个体BLCA患者的风险评分,发现其与多种临床特征密切相关,并能够准确预测BLCA患者的预后。我们相信,通过本研究,对单个样本中铜死亡介导模式的定量分析可能有助于提高我们对BLCA多组学特征的理解,并指导未来的治疗方案。Cuproptosis is a special form of cell death. Bladder cancer, especially Bladder Urothelial Carcinoma (BLCA), is one of the ten most common cancer types in the world. So far, the potential role of cuproptosis in BLCA is unclear. In the present study, we systematically evaluated the copper poisoning-mediated patterns of 509 BLCA samples based on 19 validated copper poisoning-related genes (CRGs) using data downloaded from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database. Kaplan Meier method was used to analyze the overall survival rate (OS) of different risk groups. Gene Set Variation Analysis (GSVA) was used to study the functional differences between different cuproptosis clusters (CRG clusters). Single sample gene set enrichment analysis (ssGSEA) was used to explore the potential relationship between CRG clusters and immune status. We used GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis to study various cellular biochemical processes. Finally, we established a prognostic model to predict patients’ survival outcomes and to further analyze the correlation between the predictive characteristics of BLCA patients and various treatment responses. In this study, we derived two CRG clusters and gene clusters and also established a model to quantify the risk score of individual BLCA patients, which was found to be closely associated with various clinical characteristics and could precisely predict the prognosis of BLCA patients. We believe that through our study, quantitative analysis of cuproptosis-mediated patterns in a single sample may help to improve our understanding of the multi-omics characteristics of BLCA and guide future treatment regimens.
文摘上尿路结石相关性尿脓毒血症是一种危及生命的泌尿系统急症,尤以老年患者及伴有基础疾病者为高危人群。近年来,随着结石处理技术的进步,其发生率和预后有所变化。本文系统综述了该疾病的发病机制、诊断方法及治疗策略,强调早期诊断和及时干预的重要性,并探讨不同治疗手段对患者结局的影响。在现有研究的基础上,提出优化治疗方案的建议,以期为临床诊疗提供理论依据与实践指导。Upper urinary tract stone-associated urosepsis is a life-threatening urological emergency, particularly prevalent among elderly patients and those with underlying comorbidities. In recent years, advancements in stone management techniques have led to changes in its incidence and prognosis. This review systematically summarizes the pathogenesis, diagnostic methods, and therapeutic strategies of this condition, emphasizing the importance of early diagnosis and timely intervention. Furthermore, it explores the impact of various treatment modalities on patient outcomes. Based on existing studies, recommendations are proposed to optimize treatment protocols, aiming to provide theoretical support and practical guidance for clinical practice.
文摘上尿路结石相关自发性肾破裂是泌尿外科少见的危急重症,临床表现缺乏特异性,且目前无明确的诊疗规范,临床医生常因为病因判断错误而影响后续治疗。故本文围绕该病的发病机制、临床特征和诊疗现状做一综述,以期为该病的诊疗和研究提供参考。Spontaneous renal rupture linked to upper urinary tract calculi is a rare but critical emergency in the field of urology. The non-specific nature of its clinical manifestations, coupled with the lack of standardized diagnostic and treatment guidelines, frequently leads to misdiagnosis and suboptimal treatment outcomes. This detailed review delves into the pathophysiology, clinical features, and existing diagnostic and treatment strategies for this condition, aiming to enhance clinical practice and inform future research directions.
