多重单细胞蛋白组技术已成为生物医学研究的热点,其中质谱流式成像(imaging mass cytometry,IMC)彻底解决了荧光基团间严重的串色问题,并弥补了单细胞测序技术缺失的组织空间信息。该技术能够在单张组织切片上同时标记数十种靶标,并在...多重单细胞蛋白组技术已成为生物医学研究的热点,其中质谱流式成像(imaging mass cytometry,IMC)彻底解决了荧光基团间严重的串色问题,并弥补了单细胞测序技术缺失的组织空间信息。该技术能够在单张组织切片上同时标记数十种靶标,并在单细胞层面获得它们的表达水平及细胞定位,进行深度的细胞表型原位分析并从时间和空间水平直观描绘出单细胞蛋白组图谱,因其独特的技术优势逐渐成为肿瘤研究领域新兴的强有力工具。本文将详细介绍IMC的技术原理,并通过分析近期应用案例阐述其在细胞表型鉴定、生物标志物检测、肿瘤免疫调节判定、肿瘤异质性区分、临床预后指导及反应预测等肿瘤研究方面的应用进展,推动肿瘤空间组学与现有技术的进一步结合。展开更多
近年来,肿瘤患者在接受化疗后所出现的心肌改变引起了临床和研究者的广泛关注。化疗药物的心脏毒性不仅影响患者的生活质量,还可能影响其肿瘤治疗的效果,因此深入了解其机制和影响至关重要。超声作为一种无创、实时的影像学检查手段,能...近年来,肿瘤患者在接受化疗后所出现的心肌改变引起了临床和研究者的广泛关注。化疗药物的心脏毒性不仅影响患者的生活质量,还可能影响其肿瘤治疗的效果,因此深入了解其机制和影响至关重要。超声作为一种无创、实时的影像学检查手段,能够有效评估心肌功能和结构变化,为监测化疗后心肌改变提供了重要的技术支持。目前的研究显示,超声检测在早期识别心肌损伤、评估心功能以及指导临床管理方面具有重要价值。尽管超声技术在临床应用中取得了一定的进展,仍存在标准化评估方法不足、不同化疗药物引起的心肌改变机制缺乏深入探讨等问题。本文旨在综述肿瘤化疗对心肌的影响、超声检测的应用现状及最新研究成果,以探讨超声在监测化疗后心肌改变中的潜力及其未来发展方向,为临床实践提供参考。In recent years, the myocardial changes observed in cancer patients after chemotherapy have attracted widespread attention from clinicians and researchers. The cardiotoxicity of chemotherapy drugs not only affects the quality of life of patients, but may also impact the effectiveness of their cancer treatment, making it crucial to gain a deeper understanding of its mechanisms and effects. Ultrasound, as a non-invasive and real-time imaging examination method, can effectively assess myocardial function and structural changes, providing important technical support for monitoring myocardial changes after chemotherapy. Current research shows that ultrasound detection has significant value in the early identification of myocardial injury, assessment of cardiac function, and guidance of clinical management. However, despite some progress in the clinical application of ultrasound technology, there are still issues, such as insufficient standardized assessment methods and a lack of in-depth exploration of the mechanisms of myocardial changes caused by different chemotherapy drugs. This article aims to review the impact of cancer chemotherapy on the myocardium, the current application status of ultrasound detection, and the latest research findings, in order to explore the potential of ultrasound in monitoring myocardial changes after chemotherapy and its future development directions, providing a reference for clinical practice.展开更多
文摘多重单细胞蛋白组技术已成为生物医学研究的热点,其中质谱流式成像(imaging mass cytometry,IMC)彻底解决了荧光基团间严重的串色问题,并弥补了单细胞测序技术缺失的组织空间信息。该技术能够在单张组织切片上同时标记数十种靶标,并在单细胞层面获得它们的表达水平及细胞定位,进行深度的细胞表型原位分析并从时间和空间水平直观描绘出单细胞蛋白组图谱,因其独特的技术优势逐渐成为肿瘤研究领域新兴的强有力工具。本文将详细介绍IMC的技术原理,并通过分析近期应用案例阐述其在细胞表型鉴定、生物标志物检测、肿瘤免疫调节判定、肿瘤异质性区分、临床预后指导及反应预测等肿瘤研究方面的应用进展,推动肿瘤空间组学与现有技术的进一步结合。
文摘近年来,肿瘤患者在接受化疗后所出现的心肌改变引起了临床和研究者的广泛关注。化疗药物的心脏毒性不仅影响患者的生活质量,还可能影响其肿瘤治疗的效果,因此深入了解其机制和影响至关重要。超声作为一种无创、实时的影像学检查手段,能够有效评估心肌功能和结构变化,为监测化疗后心肌改变提供了重要的技术支持。目前的研究显示,超声检测在早期识别心肌损伤、评估心功能以及指导临床管理方面具有重要价值。尽管超声技术在临床应用中取得了一定的进展,仍存在标准化评估方法不足、不同化疗药物引起的心肌改变机制缺乏深入探讨等问题。本文旨在综述肿瘤化疗对心肌的影响、超声检测的应用现状及最新研究成果,以探讨超声在监测化疗后心肌改变中的潜力及其未来发展方向,为临床实践提供参考。In recent years, the myocardial changes observed in cancer patients after chemotherapy have attracted widespread attention from clinicians and researchers. The cardiotoxicity of chemotherapy drugs not only affects the quality of life of patients, but may also impact the effectiveness of their cancer treatment, making it crucial to gain a deeper understanding of its mechanisms and effects. Ultrasound, as a non-invasive and real-time imaging examination method, can effectively assess myocardial function and structural changes, providing important technical support for monitoring myocardial changes after chemotherapy. Current research shows that ultrasound detection has significant value in the early identification of myocardial injury, assessment of cardiac function, and guidance of clinical management. However, despite some progress in the clinical application of ultrasound technology, there are still issues, such as insufficient standardized assessment methods and a lack of in-depth exploration of the mechanisms of myocardial changes caused by different chemotherapy drugs. This article aims to review the impact of cancer chemotherapy on the myocardium, the current application status of ultrasound detection, and the latest research findings, in order to explore the potential of ultrasound in monitoring myocardial changes after chemotherapy and its future development directions, providing a reference for clinical practice.
