To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro ...To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro Methods Production of NO in ECV304 cells was measured by the Griess method and the proli feration of cells was tested by the MTT method The activity of NO synthase was detected spectrophotometrically Results Production of NO in ECV304 cells decreased after treatment with 40?ng/ml IL 1 β and 40?ng/ml TNFα, but increased in the presence of H 2O 2 0 1?mmol/L PS916 significantly enhanced NO production in ECV304 cells in a dose depende nt manner in the TNFα and IL 1β treated groups and decreased it in the H 2O 2 treated group Proliferation of ECV304 cells was inhibited by TNFα and H 2O 2 and no effect was found in the IL 1β treated group PS916 increased the proliferation of cells treated with TNFα and H 2O 2 dose dependently In vitro, PS916 has no effect on the activity of NO synthase Conclusion PS916 has a protective effect on ECV304 cells exposed to IL 1β, TNFα and H 2 O 2展开更多
To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro ...To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro Methods Production of NO in ECV304 cells was measured by the Griess method and the proli feration of cells was tested by the MTT method The activity of NO synthase was detected spectrophotometrically Results Production of NO in ECV304 cells decreased after treatment with 40?ng/ml IL 1 β and 40?ng/ml TNFα, but increased in the presence of H 2O 2 0 1?mmol/L PS916 significantly enhanced NO production in ECV304 cells in a dose depende nt manner in the TNFα and IL 1β treated groups and decreased it in the H 2O 2 treated group Proliferation of ECV304 cells was inhibited by TNFα and H 2O 2 and no effect was found in the IL 1β treated group PS916 increased the proliferation of cells treated with TNFα and H 2O 2 dose dependently In vitro, PS916 has no effect on the activity of NO synthase Conclusion PS916 has a protective effect on ECV304 cells exposed to IL 1β, TNFα and H 2 O 2展开更多
基金theNationalNaturalScienceFoundationofChina (No 3 0 0 1161940 )
文摘To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro Methods Production of NO in ECV304 cells was measured by the Griess method and the proli feration of cells was tested by the MTT method The activity of NO synthase was detected spectrophotometrically Results Production of NO in ECV304 cells decreased after treatment with 40?ng/ml IL 1 β and 40?ng/ml TNFα, but increased in the presence of H 2O 2 0 1?mmol/L PS916 significantly enhanced NO production in ECV304 cells in a dose depende nt manner in the TNFα and IL 1β treated groups and decreased it in the H 2O 2 treated group Proliferation of ECV304 cells was inhibited by TNFα and H 2O 2 and no effect was found in the IL 1β treated group PS916 increased the proliferation of cells treated with TNFα and H 2O 2 dose dependently In vitro, PS916 has no effect on the activity of NO synthase Conclusion PS916 has a protective effect on ECV304 cells exposed to IL 1β, TNFα and H 2 O 2
文摘To investigate the effect of polysaccharide sulfate 916 (PS916) on the productio n of nitric oxide (NO) in ECV304 cells induced by tumor necrosis factor α (TNF α), interleukin 1β (IL 1β) and H 2O 2 in vitro Methods Production of NO in ECV304 cells was measured by the Griess method and the proli feration of cells was tested by the MTT method The activity of NO synthase was detected spectrophotometrically Results Production of NO in ECV304 cells decreased after treatment with 40?ng/ml IL 1 β and 40?ng/ml TNFα, but increased in the presence of H 2O 2 0 1?mmol/L PS916 significantly enhanced NO production in ECV304 cells in a dose depende nt manner in the TNFα and IL 1β treated groups and decreased it in the H 2O 2 treated group Proliferation of ECV304 cells was inhibited by TNFα and H 2O 2 and no effect was found in the IL 1β treated group PS916 increased the proliferation of cells treated with TNFα and H 2O 2 dose dependently In vitro, PS916 has no effect on the activity of NO synthase Conclusion PS916 has a protective effect on ECV304 cells exposed to IL 1β, TNFα and H 2 O 2
