Objective Identify the carotenoid degradation products in Undaria pinnatifida and evaluate their anti skin inflammatory activity.Methods Carotenoid degradation products were isolated by multiple chromatography methods...Objective Identify the carotenoid degradation products in Undaria pinnatifida and evaluate their anti skin inflammatory activity.Methods Carotenoid degradation products were isolated by multiple chromatography methods and their structures were identified by spectroscopic methods such as nuclear magnetic resonance(NMR)and ESI-MS.The anti-inflammatory effect of these products was evaluated using lipopolysaccharide(LPS)-stimulated RAW264.7 cell model,LPS-RAW264.7/CM-induced HacaT or HUVEC cell model.Results Five compounds were isolated from U.pinnatifida and identified as 3β-hydroxy-5α,6α-epoxy-β-ionone(1),(-)-loliolide(2),(+)-epiloliolide(3),grasshopper ketone(4),and apo-9"-fucoxanthinone(5),respectively.Compounds 1-5 exerted different anti-inflammatory effects through reducing the levels of NO,TNF-α,and IL-6 in different cell models.Conclusion Five carotenoid degradation compounds from U.pinnatifida inhibited the release of inflammatory cytokines in inflammatory cells,and reduced the damage of inflammatory cytokines to epidermal cells and vascular endothelial cells.展开更多
文摘Objective Identify the carotenoid degradation products in Undaria pinnatifida and evaluate their anti skin inflammatory activity.Methods Carotenoid degradation products were isolated by multiple chromatography methods and their structures were identified by spectroscopic methods such as nuclear magnetic resonance(NMR)and ESI-MS.The anti-inflammatory effect of these products was evaluated using lipopolysaccharide(LPS)-stimulated RAW264.7 cell model,LPS-RAW264.7/CM-induced HacaT or HUVEC cell model.Results Five compounds were isolated from U.pinnatifida and identified as 3β-hydroxy-5α,6α-epoxy-β-ionone(1),(-)-loliolide(2),(+)-epiloliolide(3),grasshopper ketone(4),and apo-9"-fucoxanthinone(5),respectively.Compounds 1-5 exerted different anti-inflammatory effects through reducing the levels of NO,TNF-α,and IL-6 in different cell models.Conclusion Five carotenoid degradation compounds from U.pinnatifida inhibited the release of inflammatory cytokines in inflammatory cells,and reduced the damage of inflammatory cytokines to epidermal cells and vascular endothelial cells.
