目的运用复杂网络方法探析针灸治疗癫痫的核心腧穴及配伍规律。方法检索中国知网、维普、万方、Web of Science、EMBASE及Pubmed数据库,依据纳入与排除标准筛选文献并建立处方数据库。运用SPSS Modeler软件分析腧穴频次与关联性,运用Gep...目的运用复杂网络方法探析针灸治疗癫痫的核心腧穴及配伍规律。方法检索中国知网、维普、万方、Web of Science、EMBASE及Pubmed数据库,依据纳入与排除标准筛选文献并建立处方数据库。运用SPSS Modeler软件分析腧穴频次与关联性,运用Gephi0.10.1软件建立复杂网络模型,探析针灸治疗癫痫处方核心腧穴与选穴规律。结果最终纳入有效文献144篇,提取199个处方,涉及102个腧穴。百会穴使用频次最高,特定穴以五腧穴、八脉交会穴、背俞穴为主,经络选择上督脉选择的腧穴最多。关联规则分析显示,百会-太冲支持度及置信度最高。复杂网络拓扑结构分析表明,百会、大椎、腰奇、丰隆等36个腧穴为针灸治疗癫痫的核心腧穴。腧穴社团分析显示,督脉循经治痫群、四肢头部远近配穴群及脏腑津液辨证群为针灸治疗癫痫的3大腧穴群。结论针灸治疗癫痫的腧穴配伍要以督脉为主结合脏腑津液辨证选穴,要注重远近配穴的选穴思路。展开更多
Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a...Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a FSN group(40 cases)and a conventional acupuncture group(40 cases)according to random number table.Within 1 week of treatment,FSN was used once every two days in the FSN group.The needle tip of Fu’s subcutaneous needle was inserted toward the affected muscle,about 5 mm in depth,at the angle of 15 to 25°.After the needle body lifted slightly,the needle went forward subcutaneously for 25 to 35 mm in depth and was swiped side to side horizontally with even exertion for about 1 min.According to the distribution of affected muscle,the reperfusion approach was repeated for 3 times consecutively.In the conventional acupuncture group conventional acupuncture was given,once a day,for 6 times totally.The scores of Western Ontario and McMaster universities osteoarthritis index(WOMAC)and Lysholm knee scoring scale(Lysholm)were compared between the two groups before and after 1-week treatment.The clinical effect of the two groups was analyzed statistically and evaluated.Results:After 1-week treatment with FSN,in the FSN group,the score of WOMACpain was reduced to be7.7±1.9 from 18.5±3.2,the score of WOMACstiffnessto be 1.5±0.7 from 4.5±1.8,WOMACfunction to be22.7±3.9 from 45.7±2.7 and the score of WOMACoverall to be 31.9±5.3 from 69.7±6.5.The differences were significant in comparison before and after treatment(all P<0.05).In the conventional acupuncture group,after 1-week treatment with conventional acupuncture,the score of WOMACpain was reduced to be11.3±2.8 from 18.2±3.0,the score of WOMACstiffness to be 3.0±1.6 from 5.6±1.7,WOMACfunction to be29.8±5.1 from 44.3±2.9 and the score of WOMACoverall to be 44.1±7.8 from 69.1±7.3.The differences were significant in comparison before and after treatment(all P<0.05).WOMAC score of every item in the FSN group was lower obviously than that in the conventional acupuncture group(P<0.01).After 1-week treatment,Lysholm score was increased to be 78.52±18.4 from 59.64±18.3 in the FSN group and it was to be 69.27±11.9 from 58.17±12.5 in the conventional acupuncture group.The differences were significant in comparison before and after treatment in either group(both P<0.05).Lysholm score in the FSN group was higher than that in the conventional acupuncture group(P<0.05).The total effective rate was 92.5%in the FSN group,higher than 77.5%in the conventional acupuncture group(P<0.05).Conclusion:Fu’s subcutaneous needling therapy effectively relieves the clinical symptoms of KOA and improves knee joint function.The therapeutic effect is better than that of the regular acupuncture.展开更多
文摘目的运用复杂网络方法探析针灸治疗癫痫的核心腧穴及配伍规律。方法检索中国知网、维普、万方、Web of Science、EMBASE及Pubmed数据库,依据纳入与排除标准筛选文献并建立处方数据库。运用SPSS Modeler软件分析腧穴频次与关联性,运用Gephi0.10.1软件建立复杂网络模型,探析针灸治疗癫痫处方核心腧穴与选穴规律。结果最终纳入有效文献144篇,提取199个处方,涉及102个腧穴。百会穴使用频次最高,特定穴以五腧穴、八脉交会穴、背俞穴为主,经络选择上督脉选择的腧穴最多。关联规则分析显示,百会-太冲支持度及置信度最高。复杂网络拓扑结构分析表明,百会、大椎、腰奇、丰隆等36个腧穴为针灸治疗癫痫的核心腧穴。腧穴社团分析显示,督脉循经治痫群、四肢头部远近配穴群及脏腑津液辨证群为针灸治疗癫痫的3大腧穴群。结论针灸治疗癫痫的腧穴配伍要以督脉为主结合脏腑津液辨证选穴,要注重远近配穴的选穴思路。
文摘目的观察穴位埋线对癫痫大鼠认知及PI3K-AKT信号通路的影响。方法80只SD雄鼠随机分为空白组、模型组、西药组及埋线组,每组20只。模型制造成功后分别进行相应干预共28 d,预定时间行水迷宫实验及处死取海马行TUNNEL、Western Blot法检测PI3K-AKT信号通路相关因子PI3K及Akt的蛋白表达的情况。结果Morris水迷宫实验:模型组大鼠潜伏期明显增多,空间探索实验中模型组的穿越平台数明显少于空白组(P<0.05);西药组及埋线组大鼠的潜伏期时间较模型组均明显缩短,穿越平台数均明显增多(P<0.05)。TUNNEL染色:模型组大鼠海马TUNNEL染色阳性细胞数目明显增多(P<0.05);西药组及穴位埋线组海马TUNNEL染色阳性细胞数目均较模型组明显减少(P<0.05)。Western Blot PI3K、Akt蛋白表达:大鼠癫痫发作后模型组、埋线组和西药组的PI3K及Akt蛋白表达均快速上升,经西药及穴位埋线干预后PI3K、Akt蛋白表达均有不同程度的下降,其中以埋线组蛋白表达下降最多。结论穴位埋线可以通过激活癫痫大鼠PI3K-AKT信号通路来抗神经元细胞凋亡,减少海马神经元损伤及改善认知。
基金Supported by Outstanding Innovation Talent Support Project of Heilongjiang University of Traditional Chinese Medicine:2018RCD10:the ForthTerm National Chinese Medicine(Clinical and Basic Research)Outstanding Talent Advanced Training Program,State Administration of Traditional Chinese Medicine:National TCM Education[2017]24.
文摘Objective:To observe and compare the clinical effect on knee osteoarthritis(KOA)between Fu’s subcutaneous needling therapy(FSN)and convention acupuncture.Methods:A total of 80 outpatients with KOA were divided into a FSN group(40 cases)and a conventional acupuncture group(40 cases)according to random number table.Within 1 week of treatment,FSN was used once every two days in the FSN group.The needle tip of Fu’s subcutaneous needle was inserted toward the affected muscle,about 5 mm in depth,at the angle of 15 to 25°.After the needle body lifted slightly,the needle went forward subcutaneously for 25 to 35 mm in depth and was swiped side to side horizontally with even exertion for about 1 min.According to the distribution of affected muscle,the reperfusion approach was repeated for 3 times consecutively.In the conventional acupuncture group conventional acupuncture was given,once a day,for 6 times totally.The scores of Western Ontario and McMaster universities osteoarthritis index(WOMAC)and Lysholm knee scoring scale(Lysholm)were compared between the two groups before and after 1-week treatment.The clinical effect of the two groups was analyzed statistically and evaluated.Results:After 1-week treatment with FSN,in the FSN group,the score of WOMACpain was reduced to be7.7±1.9 from 18.5±3.2,the score of WOMACstiffnessto be 1.5±0.7 from 4.5±1.8,WOMACfunction to be22.7±3.9 from 45.7±2.7 and the score of WOMACoverall to be 31.9±5.3 from 69.7±6.5.The differences were significant in comparison before and after treatment(all P<0.05).In the conventional acupuncture group,after 1-week treatment with conventional acupuncture,the score of WOMACpain was reduced to be11.3±2.8 from 18.2±3.0,the score of WOMACstiffness to be 3.0±1.6 from 5.6±1.7,WOMACfunction to be29.8±5.1 from 44.3±2.9 and the score of WOMACoverall to be 44.1±7.8 from 69.1±7.3.The differences were significant in comparison before and after treatment(all P<0.05).WOMAC score of every item in the FSN group was lower obviously than that in the conventional acupuncture group(P<0.01).After 1-week treatment,Lysholm score was increased to be 78.52±18.4 from 59.64±18.3 in the FSN group and it was to be 69.27±11.9 from 58.17±12.5 in the conventional acupuncture group.The differences were significant in comparison before and after treatment in either group(both P<0.05).Lysholm score in the FSN group was higher than that in the conventional acupuncture group(P<0.05).The total effective rate was 92.5%in the FSN group,higher than 77.5%in the conventional acupuncture group(P<0.05).Conclusion:Fu’s subcutaneous needling therapy effectively relieves the clinical symptoms of KOA and improves knee joint function.The therapeutic effect is better than that of the regular acupuncture.
