Objective： TO investigate the anti-angiogenic effects of Pien Tze Huang （片仔癀, PZH） in vivo and in vitro. Me.otis： Human umbilical vein endothelial cells （HUVECs） were treated with 0 mg/mL, 0.25 mg/mL, 0.5 mg/...Objective： TO investigate the anti-angiogenic effects of Pien Tze Huang （片仔癀, PZH） in vivo and in vitro. Me.otis： Human umbilical vein endothelial cells （HUVECs） were treated with 0 mg/mL, 0.25 mg/mL, 0.5 mg/mL, and 1 mg/mL of PZH for 24 h, 48 h and 72 h, respectively. Chicken embryo chorioallantoic membrane （CAM） model was used to evaluate in vivo angiogenesis. An ECMatrix gel system was used to evaluate in vitro angiogenesis by examining the tube formation of HUVECs. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay was performed to determine HUVEC viability. Cell density of HUVECs was observed by phase- contrast microscopy. HUVEC migration was determined by wound healing method. The mRNA and protein expression of vascular endothelial growth factor A （VEGF-A） and basic fibroblast growth factor （bFGF） in both HUVEC and human colon adenocaminoma cells （HT-29） was examined by reverse transcription polymerase chain reaction （RT-PCR） and enzyme linked immune sorbent assay （ELISA）, respectively. Results： PZH treatment significantly reduced the total number of blood vessels compared with the untreated control in the chicken embryos and resulted in a significant decrease in capillary tube formation and cell density of HUVECs （P〈0.05）. In addition, treatment with 0.25-1 mg/mL of PZH for 24 h, 48 h, and 72 h respectively reduced cell viability by 9%-52%, 24%-87% or 25%-87%, compared with the untreated control cells （P〈0.05）. Moreover, PZH treatment decreased the migration of HUVECs. Furthermore, PZH close-dependently suppressed the expression of VEGF-A and bFGF on both mRNA and protein levels （P〈0.05）. Conclusion： PZH could inhibit angiogenesis in vivo in CAM model and in vitro on HUVECs, suggesting that inhibiting tumor angiogenesis might be one of the mechanisms by which PZH treats cancer.展开更多
Objective： To investigate the molecular mechanisms by which Qianliening Capsule （前列宁胶囊,QC） treats benign prostatic hyperplasia （BPH）. Methods： Human prostate stromal cell line WPMY1 was treated with 0, 1, 3...Objective： To investigate the molecular mechanisms by which Qianliening Capsule （前列宁胶囊,QC） treats benign prostatic hyperplasia （BPH）. Methods： Human prostate stromal cell line WPMY1 was treated with 0, 1, 3 and 5 mg/mL of QC for 24, 48 and 72 h, respectively, in the presence of 10 ng/mL basic fibroblast growth factor （bFGF）. The viability of WPMY1 cells was determined by 3（4,5Dimethylthiazol2yl）2,5diphenyltetrazolium bromide （M＇lr） assay. Cell morphology was observed by phasecontrast microscopy. 4＇,6diamidino2phenylindole （DAPI） staining and fluorescence activated cell sorting （FACS） analysis with AnnexinV/propidium iodide （PI） staining were performed to determine cell apoptosis. The loss of mitochondrial membrane potential was examined by FACS analysis with 5,5＇,6,6＇tetrachloro1 ,l＇,3,3＇tetraethylbenzimidazolylcarbocyadne iodide （JC1） staining. Activation of caspase3 and 9 was evaluated by colorimetric assay. The mRNA and protein expression levels of Bcl2 and Bax were measured by reverse transcription polymerase chain reaction （RTPCR） and Western blotting, respectively. Results： Upon bFGF stimulation, the viability of WPMY1 cells was increased to 122%118% compared with the control cells （P〈0.05）. However, treatment with 15 mg/mL of QC for 24, 48 and 72 h decreased the viability of bFGFsUmulated cells to 80%92%, 59%82%, 36%62% compared with the untreated cells （P〈0.05）. In addition, QC treatment reduced WPMY1 cell density in a dosedependent manner. Moreover, QC treatment dosedependently induced the loss of plasma membrane asymmetry, the nuclear condensation and fragmentation, collapse of mitochondrial membrane potential, activation of caspase9 and caspase3, and increase of proapoptotic Bax/Bcl2 ratio. Conclusion： Promoting mitochondriondependent apoptosis of prostate stromal cells might be one of the mechanisms by which QC treats BPH.展开更多
Objective：To study the chemical composition,anticancer,anti-neuroinflammatory,and antioxidant activities of the essential oil of Patrinia scabiosaefolia（EO-PS）.Methods：Patrinia scabiosaefolia was analyzed by gas c...Objective：To study the chemical composition,anticancer,anti-neuroinflammatory,and antioxidant activities of the essential oil of Patrinia scabiosaefolia（EO-PS）.Methods：Patrinia scabiosaefolia was analyzed by gas chromatography-mass spectrometry.Eight human carcinoma cell lines,including SGC-7901,AGS,Hep G2,HT-29,HCT-8,5-FU/HCT-8,He La,and MDA-MB-231,were assessed by methylthiazolyldiphenyltetrazolium bromide（MTT） assay.Anti-neuroinflammatory activity was assessed by production of interleukin（IL）-1β and IL-6 induced by lipopolysaccharide in BV-2 cells（microglia from mice）.The antioxidant activity was evaluated with a 2,2-diphenyl-1-picrylhydrazyl（DPPH） scavenging assay.Results：Forty-four components,representing 83.919% of the total oil,were identified in the EO-PS.The major constituents were caryophyllene oxide（12.802%）,caryophyllene（6.909%）,α-caryophyllene（2.927%）,β-damascenone（3.435%）,calarene（5.621%）,and phenol（3.044%）.The MTT assay showed that the EO-PS exhibited significant dose-dependent growth inhibition in the 50–200 μg/m L dilution range.The EO-PS exhibited a dose-dependent scavenging activity against the DPPH radical,with an half of maximal inhibitory concentration 1.455 mg/m L.Conclusion：The EO-PS possesses a wide range of antitumor,anti-neuroinflammatory and antioxidant activities,suggesting that it may be a good candidate for further investigations of new bioactive substances.展开更多
基金Surpported by the National Natural Science Foundation of China(No.81 073097)the Developmental Fund of Chen Ke-ji Integrative Medicine(No.CKJ 2011001)the Natural Science Foundation of Fujian Province of China(No.2010J01195)
文摘Objective： TO investigate the anti-angiogenic effects of Pien Tze Huang （片仔癀, PZH） in vivo and in vitro. Me.otis： Human umbilical vein endothelial cells （HUVECs） were treated with 0 mg/mL, 0.25 mg/mL, 0.5 mg/mL, and 1 mg/mL of PZH for 24 h, 48 h and 72 h, respectively. Chicken embryo chorioallantoic membrane （CAM） model was used to evaluate in vivo angiogenesis. An ECMatrix gel system was used to evaluate in vitro angiogenesis by examining the tube formation of HUVECs. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay was performed to determine HUVEC viability. Cell density of HUVECs was observed by phase- contrast microscopy. HUVEC migration was determined by wound healing method. The mRNA and protein expression of vascular endothelial growth factor A （VEGF-A） and basic fibroblast growth factor （bFGF） in both HUVEC and human colon adenocaminoma cells （HT-29） was examined by reverse transcription polymerase chain reaction （RT-PCR） and enzyme linked immune sorbent assay （ELISA）, respectively. Results： PZH treatment significantly reduced the total number of blood vessels compared with the untreated control in the chicken embryos and resulted in a significant decrease in capillary tube formation and cell density of HUVECs （P〈0.05）. In addition, treatment with 0.25-1 mg/mL of PZH for 24 h, 48 h, and 72 h respectively reduced cell viability by 9%-52%, 24%-87% or 25%-87%, compared with the untreated control cells （P〈0.05）. Moreover, PZH treatment decreased the migration of HUVECs. Furthermore, PZH close-dependently suppressed the expression of VEGF-A and bFGF on both mRNA and protein levels （P〈0.05）. Conclusion： PZH could inhibit angiogenesis in vivo in CAM model and in vitro on HUVECs, suggesting that inhibiting tumor angiogenesis might be one of the mechanisms by which PZH treats cancer.
基金Supported by the National Natural Science Foundation of China(No.81072927 and No.81173433)the Natural Science Foundation of Fujian Province of China(No.2010J01199 and No.2009J01169)
文摘Objective： To investigate the molecular mechanisms by which Qianliening Capsule （前列宁胶囊,QC） treats benign prostatic hyperplasia （BPH）. Methods： Human prostate stromal cell line WPMY1 was treated with 0, 1, 3 and 5 mg/mL of QC for 24, 48 and 72 h, respectively, in the presence of 10 ng/mL basic fibroblast growth factor （bFGF）. The viability of WPMY1 cells was determined by 3（4,5Dimethylthiazol2yl）2,5diphenyltetrazolium bromide （M＇lr） assay. Cell morphology was observed by phasecontrast microscopy. 4＇,6diamidino2phenylindole （DAPI） staining and fluorescence activated cell sorting （FACS） analysis with AnnexinV/propidium iodide （PI） staining were performed to determine cell apoptosis. The loss of mitochondrial membrane potential was examined by FACS analysis with 5,5＇,6,6＇tetrachloro1 ,l＇,3,3＇tetraethylbenzimidazolylcarbocyadne iodide （JC1） staining. Activation of caspase3 and 9 was evaluated by colorimetric assay. The mRNA and protein expression levels of Bcl2 and Bax were measured by reverse transcription polymerase chain reaction （RTPCR） and Western blotting, respectively. Results： Upon bFGF stimulation, the viability of WPMY1 cells was increased to 122%118% compared with the control cells （P〈0.05）. However, treatment with 15 mg/mL of QC for 24, 48 and 72 h decreased the viability of bFGFsUmulated cells to 80%92%, 59%82%, 36%62% compared with the untreated cells （P〈0.05）. In addition, QC treatment reduced WPMY1 cell density in a dosedependent manner. Moreover, QC treatment dosedependently induced the loss of plasma membrane asymmetry, the nuclear condensation and fragmentation, collapse of mitochondrial membrane potential, activation of caspase9 and caspase3, and increase of proapoptotic Bax/Bcl2 ratio. Conclusion： Promoting mitochondriondependent apoptosis of prostate stromal cells might be one of the mechanisms by which QC treats BPH.
基金Supported by the National Natural Science Foundation of China(No.81403390)the Provincial Natural Science Foundation of Fujian(No.2014J01352 and No.2014J01360)+1 种基金Provincial Health Department Foundation of Fujian(No.2013-2-55)Fujian University of Traditional Chinese Medicine Key Discipline Construction Foundation(Nos.X2012012,X2013014,X2013015,X2014132 and X2015020)
文摘Objective：To study the chemical composition,anticancer,anti-neuroinflammatory,and antioxidant activities of the essential oil of Patrinia scabiosaefolia（EO-PS）.Methods：Patrinia scabiosaefolia was analyzed by gas chromatography-mass spectrometry.Eight human carcinoma cell lines,including SGC-7901,AGS,Hep G2,HT-29,HCT-8,5-FU/HCT-8,He La,and MDA-MB-231,were assessed by methylthiazolyldiphenyltetrazolium bromide（MTT） assay.Anti-neuroinflammatory activity was assessed by production of interleukin（IL）-1β and IL-6 induced by lipopolysaccharide in BV-2 cells（microglia from mice）.The antioxidant activity was evaluated with a 2,2-diphenyl-1-picrylhydrazyl（DPPH） scavenging assay.Results：Forty-four components,representing 83.919% of the total oil,were identified in the EO-PS.The major constituents were caryophyllene oxide（12.802%）,caryophyllene（6.909%）,α-caryophyllene（2.927%）,β-damascenone（3.435%）,calarene（5.621%）,and phenol（3.044%）.The MTT assay showed that the EO-PS exhibited significant dose-dependent growth inhibition in the 50–200 μg/m L dilution range.The EO-PS exhibited a dose-dependent scavenging activity against the DPPH radical,with an half of maximal inhibitory concentration 1.455 mg/m L.Conclusion：The EO-PS possesses a wide range of antitumor,anti-neuroinflammatory and antioxidant activities,suggesting that it may be a good candidate for further investigations of new bioactive substances.
