For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluores...For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H2SO4 and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.展开更多
Objective: There are no detailed reports of three-dimensional measurement of abutment teeth in mastication, because it is knotty to observe the rotation in chewing directly, and inexact to estimate indirectly. This wo...Objective: There are no detailed reports of three-dimensional measurement of abutment teeth in mastication, because it is knotty to observe the rotation in chewing directly, and inexact to estimate indirectly. This work studies the three-dimensional stability of rigidly fixed bridge under the stresses of distributed loads and concentrated loads by optical method that gives the tip angle and rotation angle calculated directly based on measurement data. Methods: The specimen, taken from a 25-year-old male, was a left mandible without the second premolars and the first molars. As abutments, first premolar and second molar have complete periodontium. The specimen was soaked in formaldehyde solution. The bridge was fixed between two abutment teeth (first premolars and second molars), and the mandible was cemented in a steel box. The load was increased from 0 kg to 23 kg. Laser holographic technique was used to measure the three-dimensional bit shift of the dens, both buccolingual bit shift and mesiodistal bit shift, and determine tip angle and rotation angle. Results: The effects of stress distribution on the rigidly fixed bridge were evaluated, and stabilization of the bridge under the stresses of distributed loads and concentrated loads, respectively, were analyzed. The results showed that the tips of two abutments were very similar, and no distinct difference was observed between the distributed load and the concentrated load. However, the maximum rotation angle for the distributed load was two to four times as large as that for the concentrated load. In the experiment, the tip angle of the abutment teeth was no more than 0.65 degree, and the rotation angle was no more than 0.60 degree. All maximum angles occurred in the second molar. Conclusion: The fixed bridge is considered to be safe. In addition, a method for measuring the rotation angle was provided effectively.展开更多
基金Project supported by the National Natural Science Foundation of China (Nos.20375035 and 20527005)the Natural Science Foundation of Zhejiang Province (No.Z404105), China
文摘For preparing fluorinated quinolone antibiotic medicine locally used in stomatology, simultaneous determination of norfloxacin, ciprofloxacin, and enoxacin was carried out by multiphase ion chromatography with fluorescence detection. Quinolone antibiotics were separated by Dionex OmniPac PAX-500 column with an eluent of 15 mmol/L H2SO4 and 35% methanol (v/v) at a flow-rate of 1.0 ml/min and detected with fluorescence with excitation and emission wave lengths of 347 nm and 420 nm respectively. The detection limits (S/N=3) of norfloxacin, ciprofloxacin and enoxacin were 50, 105 and 80 ng/ml respectively. The relative standard deviations of retention time, peak area and peak height were less than 1.1% and good linear relationship resulted. The developed method was applied to pharmaceutical formulations and biological fluids.
基金the Science Research Foundation of Ministry of Health of China (No. WKJ2006-2-003)the Department of Education of Zhejiang Province (No. 20010505) the Science and Technol-ogy Department of Zhejiang Province (No. 2003C30044), China
文摘Objective: There are no detailed reports of three-dimensional measurement of abutment teeth in mastication, because it is knotty to observe the rotation in chewing directly, and inexact to estimate indirectly. This work studies the three-dimensional stability of rigidly fixed bridge under the stresses of distributed loads and concentrated loads by optical method that gives the tip angle and rotation angle calculated directly based on measurement data. Methods: The specimen, taken from a 25-year-old male, was a left mandible without the second premolars and the first molars. As abutments, first premolar and second molar have complete periodontium. The specimen was soaked in formaldehyde solution. The bridge was fixed between two abutment teeth (first premolars and second molars), and the mandible was cemented in a steel box. The load was increased from 0 kg to 23 kg. Laser holographic technique was used to measure the three-dimensional bit shift of the dens, both buccolingual bit shift and mesiodistal bit shift, and determine tip angle and rotation angle. Results: The effects of stress distribution on the rigidly fixed bridge were evaluated, and stabilization of the bridge under the stresses of distributed loads and concentrated loads, respectively, were analyzed. The results showed that the tips of two abutments were very similar, and no distinct difference was observed between the distributed load and the concentrated load. However, the maximum rotation angle for the distributed load was two to four times as large as that for the concentrated load. In the experiment, the tip angle of the abutment teeth was no more than 0.65 degree, and the rotation angle was no more than 0.60 degree. All maximum angles occurred in the second molar. Conclusion: The fixed bridge is considered to be safe. In addition, a method for measuring the rotation angle was provided effectively.
