A rapid method based on rapid resolution liquid chromatography(RRLC) coupled with a diode array detector(DAD) was developed for the simultaneous determination of six major constituents(magnoflorine,jatrorrhizine,copti...A rapid method based on rapid resolution liquid chromatography(RRLC) coupled with a diode array detector(DAD) was developed for the simultaneous determination of six major constituents(magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine) in traditional Chinese medicine(TCM) Zuo Jin Pill(ZJP).The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column(1.8 μm i.d.,150 mm×4.6 mm) by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity(r2 >0.9998).The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis(HCA) was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.展开更多
基金We thank Drs J Zhao, DS Li, L Xiao （Chinese Academy of Sciences, China）, Drs B Leo and H Wang （Agilent Technologies, USA） for helpful discussions and technical assistance, and Drs HK Mei and Y Qiu （GlaxoSmithKline, UK） for the DAVID analysis. This research was supported by the Ministry of Science and Technology （2005CB522406, 2006CB943900, 2007CB947904, 2007CB947100, 2009CB941100, and 2007CB948000）, National Natural Science Foundation of China （30621091, 30625014, 30623003, and 90713047）, Shanghai Municipal Commission for Science and Technology （07PJ14099, 06ZR14098, and 06DZ22032）, and the Chinese Academy of Sciences （KSCX2-YW-R-56 and 2007KIP204）.
基金Supported by the National Natural Science Foundation of China(No.30725045)the Special Program for New Drug Innovation of the Ministry of Science and Technology,China(Nos.2009ZX09311-001 and 2008ZX09101-Z-029)+2 种基金China Postdoctoral Science Foundation(No.20080441304)Shanghai Leading Academic Discipline Project,China(No.B906)the Scientific Foundation of Shanghai City,China(Nos.07DZ19728,09DZ1975700 and 09DZ1971500)
文摘A rapid method based on rapid resolution liquid chromatography(RRLC) coupled with a diode array detector(DAD) was developed for the simultaneous determination of six major constituents(magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine) in traditional Chinese medicine(TCM) Zuo Jin Pill(ZJP).The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column(1.8 μm i.d.,150 mm×4.6 mm) by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity(r2 >0.9998).The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis(HCA) was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM.
