A bioactive compound from Paecilomyces tenuipes (BCPT) has an inhibitory effect on monoamine oxidase A (MAO-A) in vitro. Researchers have thought that BCPT may be a potential antidepressant. The MAO-A suppressor moclo...A bioactive compound from Paecilomyces tenuipes (BCPT) has an inhibitory effect on monoamine oxidase A (MAO-A) in vitro. Researchers have thought that BCPT may be a potential antidepressant. The MAO-A suppressor moclobemide served as a control, and this study investigated the mechanisms of BCPT as an antidepressant. Results demonstrated that BCPT induced significantly increased sucrose intake in chronic unpredictable stressed rats, shortened immobility time in forced swimming mice, improved the scores of blepharoptosis and akinesia in reserpine-treated mice, increased the number of 5-hydroxy tryptophan-induced head-twitches, remarkably enhanced the expression of hippocampus mineralcorticoid receptor and glucocorticoid receptor mRNA, decreased the ratio of mineralcorticoid receptor to glucocorticoid receptor and raised the levels of dopamine, norepinephrine and 5-hydroxytryptamine, while decreasing hydroxyindole acetic acid levels or dihydroxy-phenyl acetic acid in chronic unpredictable stressed rats. Behavioral test results suggested that BCPT potentially had antidepressant-like activity. Meanwhile, BCPT increased the levels of neurotransmitters, and mineralcorticoid receptor and glucocorticoid receptor mRNA in the hippocampus, which may be an important mechanism of its antidepressant effect.展开更多
Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effe...Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-κB and tumor necrosis factor-α and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction (200 mg/kg) exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction (100 mg/L) exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis.展开更多
基金the Grant of Anhui Education Department, No. 2004KJ194ZDthe Grant of Anhui Science & Technology Department, No. 04023048
文摘A bioactive compound from Paecilomyces tenuipes (BCPT) has an inhibitory effect on monoamine oxidase A (MAO-A) in vitro. Researchers have thought that BCPT may be a potential antidepressant. The MAO-A suppressor moclobemide served as a control, and this study investigated the mechanisms of BCPT as an antidepressant. Results demonstrated that BCPT induced significantly increased sucrose intake in chronic unpredictable stressed rats, shortened immobility time in forced swimming mice, improved the scores of blepharoptosis and akinesia in reserpine-treated mice, increased the number of 5-hydroxy tryptophan-induced head-twitches, remarkably enhanced the expression of hippocampus mineralcorticoid receptor and glucocorticoid receptor mRNA, decreased the ratio of mineralcorticoid receptor to glucocorticoid receptor and raised the levels of dopamine, norepinephrine and 5-hydroxytryptamine, while decreasing hydroxyindole acetic acid levels or dihydroxy-phenyl acetic acid in chronic unpredictable stressed rats. Behavioral test results suggested that BCPT potentially had antidepressant-like activity. Meanwhile, BCPT increased the levels of neurotransmitters, and mineralcorticoid receptor and glucocorticoid receptor mRNA in the hippocampus, which may be an important mechanism of its antidepressant effect.
基金supported by a grant from the Major Programs of Anhui Science and Technology Special Funds,No.08010302099the Doctor Funds of Anhui Medical University,No.XJ200813
文摘Buyang Huanwu Decoction fraction extracted from Buyang Huanwu Decoction contains saponins of Astragalus, total paeony glycoside and safflower flavones. The aim of this study was to demonstrate the neuroprotective effect and mechanism of Buyang Huanwu Decoction fraction on ischemic injury both in vivo and in vitro. In vivo experiments showed that 50-200 mg/kg Buyang Huanwu Decoction fraction reduced infarct volume and pathological injury in ischemia/reperfusion rats, markedly inhibited expression of nuclear factor-κB and tumor necrosis factor-α and promoted nestin protein expression in brain tissue. Buyang Huanwu Decoction fraction (200 mg/kg) exhibited significant effects, which were similar to those of 100 mg/kg Ginkgo biloba extract. In vitro experimental results demonstrated that 10-100 mg/L Buyang Huanwu Decoction fraction significantly improved cell viability, decreased the release of lactate dehydrogenase and malondialdehyde levels, and inhibited the rate of apoptosis in HT22 cells following oxygen-glucose deprivation. Buyang Huanwu Decoction fraction (100 mg/L) exhibited significant effects, which were similar to those of 100 mg/L Ginkgo biloba extract. These findings suggest that Buyang Huanwu Decoction fraction may represent a novel, protective strategy against cerebral ischemia/reperfusion injury in rats and oxygen-glucose deprivation-induced damage in HT22 cells in vitro by attenuating the inflammatory response and cellular apoptosis.
