Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaite...Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaited.A series of methods have been explored to make single-cell proteomics available.The design of nanoPOTS and OAD chip-based processes minimizes sample preparation steps and volumes to reduce sample loss[2,3].展开更多
基金supported by the National Key R&D Program of China(2020YFE0202200)the National Natural Science Foundation of China(82070365)the Basic Research General Program of Shenzhen(JCYJ20190807103407873)。
文摘Since a typical mammalian cell contains only 200–300 picograms of proteins and the lack of a proteome amplification strategy[1],a practical and high-throughput approach for single-cell proteomics has long been awaited.A series of methods have been explored to make single-cell proteomics available.The design of nanoPOTS and OAD chip-based processes minimizes sample preparation steps and volumes to reduce sample loss[2,3].
