Introduction: Resistance to antituberculosis drugs and adverse drug reactions remain the leading causes of tuberculosis therapeutic failure globally. Despite the increasing acceptance of medicinal plant use in combina...Introduction: Resistance to antituberculosis drugs and adverse drug reactions remain the leading causes of tuberculosis therapeutic failure globally. Despite the increasing acceptance of medicinal plant use in combination with conventional antituberculosis drugs in treatment of tuberculosis (TB) in Uganda, there is paucity of knowledge on their combination effect. Aim: This research aimed to determine combination activity of standard antituberculosis drugs with extracts of Zanthoxylum leprieurii Guill. & Perr. and Rubia cordifolia L., the two common antituberculosis medicinal plants in Uganda, against pansensitive (H37Rv) and multi-drug resistant (MDR) Mycobacterium tuberculosis strains. Materials and Methods: Two reference MTB strains (H37Rv and MDR strain) were inoculated on Middlebrook 7H11 medium containing a combination of standard antituberculosis drugs and methanol extracts of Z. leprieurii and R. cordifolia at varying concentrations. The number of colonies on the plates was observed and counted weekly for up to 8 weeks. In vitro combination activity was determined using proportion method. Mean percentage inhibition was calculated for the reduction of number of colonies on drug-extract combination medium in relation to drug-extract-free control medium. Results: Drug-extract combinations showed good combination activity against Mycobacterium tuberculosis strains when compared with individual standard anti-TB drugs. This was more exhibited against MDR strain. There was however a reduction in percentage inhibition when extracts were combined with ethambutol and streptomycin against H37Rv strain. Conclusions: Zanthoxylum leprieurii and Rubia cordifolia in combination with standard anti-TB drugs exhibited increased in vitro activity against Mycobacterium tuberculosis, especially MDR-TB strain. This justifies the local use of these plants in traditional treatment of tuberculosis especially in resistant cases in Uganda.展开更多
目的探讨液相芯片技术快速检测呼吸道病原体的可行性,为重大传染病原体核酸快速应急检测平台的建立提供实验依据。方法采用xTAG~?Respiratory Viral Panel Fast v2试剂盒对74例咽拭子临床标本进行检测,结果与实时荧光定量PCR(RT-PCR)进...目的探讨液相芯片技术快速检测呼吸道病原体的可行性,为重大传染病原体核酸快速应急检测平台的建立提供实验依据。方法采用xTAG~?Respiratory Viral Panel Fast v2试剂盒对74例咽拭子临床标本进行检测,结果与实时荧光定量PCR(RT-PCR)进行比较。结果 74例咽拭子临床标本经液相芯片技术与RT-PCR检测,阳性率分别为77.0%和68.9%,差异有统计学意义(P<0.05);其中呼吸道合胞病毒、鼻病毒、肠道病毒、甲型H1N1流感病毒、腺病毒及人博卡病毒检出率差异均有统计学意义(P<0.05)。结论液相芯片技术可以较准确地鉴定呼吸道病原体和检测混合感染的临床标本,具有快速、高通量等优势,可应用于呼吸道病原体的快速应急检测。展开更多
目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最...目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最低检出限、线性范围和线性相关系数。收集来自南部战区疾病预防控制中心生物安全科2016年至2018年的临床样本278例(IAV阳性样本88例,IBV阳性样本87例,阴性样本103例),其中男性164人(59%),女性114人(41%),平均年龄24岁,通过重复检测相同IAV、IBV临床样本评价其分析重现性;将系统检测278例鼻/咽拭子临床样本与传统实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)结果比较评价其效果。结果极速热循环荧光定量PCR系统检测IAV和IBV质粒标准品的最低检出限为1×10^(0)copies/μL;线性检测范围是1×10^(0)~1×10^(5)copies/μL;此系统检测流感临床样本的敏感度为92.57%,特异度均为100%,阴、阳性预测值分别为88.79%和100%,与RT-qPCR结果的符合率为95.32%;IAV与IBV之间以及IAV和IBV与其他多种病原体之间无交叉反应;30 g/dL的血红蛋白、3.2 g/dL的甘油三酯和100μg/mL头孢曲松对检测结果无干扰。结论极速热循环荧光定量PCR系统体积小、重量轻、无需核酸提取步骤、耗时短、检测性能良好,适用于即时检测(point-of-care testing,POCT)。展开更多
Compassion is one of the core fundamental elements of Buddhism. The compassionate virtues are the precious qualities essential in the practice of Buddhism. On the other hand, guanxi plays a dominant role in any succes...Compassion is one of the core fundamental elements of Buddhism. The compassionate virtues are the precious qualities essential in the practice of Buddhism. On the other hand, guanxi plays a dominant role in any successful business venture in Chinese market. This paper develops the understanding of the two ancient bodies of knowledge of Buddhism and Confucianism. The attempt will be made in this paper to understand the deeper meaning of compassion in Buddhism in terms of compassionate virtues such as generosity, discipline, patience, diligence, humility, and wisdom. In guanxi, it will delve into different aspects of guanxi in terms of its background, meaning, significance, different types, intermediary, comparison with networking and ethics and success factors. This paper is based on the literature review on compassion--guanxi. The compassion literature focuses on books on Buddhism and commentaries by great Tibetan Buddhist sages. The guanxi literatures are predominately based on business research papers related to guanxi and business culture in China. The paper provides deeper meaning of compassion in Buddhism. The application of compassion to manage guanxi in China is the topic of this paper. This research is about optimizing the art of guanxi through the application of compassion which will help many non-Chinese business managers to effectively manage it. This paper is considered the first of its kind to study compassion in Buddhism and guanxi practice in China and seeks to provide the necessary framework to conduct the further research on applying compassion to build and manage better guanxi in China.展开更多
This paper report the binding pattern of 12 different kinds of lectin in human breast lesions. Of the 12 kinds of lectins, WGA showed the highest binding activity to the cells of the breast tissue derived; the binding...This paper report the binding pattern of 12 different kinds of lectin in human breast lesions. Of the 12 kinds of lectins, WGA showed the highest binding activity to the cells of the breast tissue derived; the binding of BSL, SBA and DBA were localized to membrane or cytoplasm of cancer cells and to the lumina membrane border of the normal and benign lesions; PNA receptor is related with the differentiation of the breast; cancer; we didn't find any relationship between the lectin receptor and the tendency of metastasis of breast cancer.展开更多
Eight lectins were used to study 100 cases of breast carcinoma and 56 cases of non-carcinoma breast tissues by lectin affinity histochemical method. The results showed that Bandeirasa Simplicifolia (BSL) and Peanut ag...Eight lectins were used to study 100 cases of breast carcinoma and 56 cases of non-carcinoma breast tissues by lectin affinity histochemical method. The results showed that Bandeirasa Simplicifolia (BSL) and Peanut agglutinin (PNA) had higher positive rates in breast carcinoma than both normal breast and benign lesions (P<0.005). The positive deposit in malignant lesions was mainly located in cytoplasm, while in non-malignant lesions, it was almost lined along the lumen of glands and small ducts (P<0.005). The authors think that expression of PNA-receptor in the cytoplasm might be associated with the mechanism that the tumor could escape from immune attack. Comparison analysis on the normal breast indicated that PNA affinity histoche-mistry would be useful to the understanding of the metabolism of β-D-galactosyl-N-acetyl-D-galactosa-mine during the development of normal breast and histological origin of breast carcinoma.展开更多
文摘Introduction: Resistance to antituberculosis drugs and adverse drug reactions remain the leading causes of tuberculosis therapeutic failure globally. Despite the increasing acceptance of medicinal plant use in combination with conventional antituberculosis drugs in treatment of tuberculosis (TB) in Uganda, there is paucity of knowledge on their combination effect. Aim: This research aimed to determine combination activity of standard antituberculosis drugs with extracts of Zanthoxylum leprieurii Guill. & Perr. and Rubia cordifolia L., the two common antituberculosis medicinal plants in Uganda, against pansensitive (H37Rv) and multi-drug resistant (MDR) Mycobacterium tuberculosis strains. Materials and Methods: Two reference MTB strains (H37Rv and MDR strain) were inoculated on Middlebrook 7H11 medium containing a combination of standard antituberculosis drugs and methanol extracts of Z. leprieurii and R. cordifolia at varying concentrations. The number of colonies on the plates was observed and counted weekly for up to 8 weeks. In vitro combination activity was determined using proportion method. Mean percentage inhibition was calculated for the reduction of number of colonies on drug-extract combination medium in relation to drug-extract-free control medium. Results: Drug-extract combinations showed good combination activity against Mycobacterium tuberculosis strains when compared with individual standard anti-TB drugs. This was more exhibited against MDR strain. There was however a reduction in percentage inhibition when extracts were combined with ethambutol and streptomycin against H37Rv strain. Conclusions: Zanthoxylum leprieurii and Rubia cordifolia in combination with standard anti-TB drugs exhibited increased in vitro activity against Mycobacterium tuberculosis, especially MDR-TB strain. This justifies the local use of these plants in traditional treatment of tuberculosis especially in resistant cases in Uganda.
文摘目的探讨极速热循环荧光定量PCR系统在甲型流感病毒(influenza A virus,IAV)和乙型流感病毒(influenza B virus,IBV)检测中的性能。方法构建IAV和IBV的质粒标准品,浓度梯度稀释,每个浓度重复检测20次后,制作浓度标准曲线,评价系统的最低检出限、线性范围和线性相关系数。收集来自南部战区疾病预防控制中心生物安全科2016年至2018年的临床样本278例(IAV阳性样本88例,IBV阳性样本87例,阴性样本103例),其中男性164人(59%),女性114人(41%),平均年龄24岁,通过重复检测相同IAV、IBV临床样本评价其分析重现性;将系统检测278例鼻/咽拭子临床样本与传统实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)结果比较评价其效果。结果极速热循环荧光定量PCR系统检测IAV和IBV质粒标准品的最低检出限为1×10^(0)copies/μL;线性检测范围是1×10^(0)~1×10^(5)copies/μL;此系统检测流感临床样本的敏感度为92.57%,特异度均为100%,阴、阳性预测值分别为88.79%和100%,与RT-qPCR结果的符合率为95.32%;IAV与IBV之间以及IAV和IBV与其他多种病原体之间无交叉反应;30 g/dL的血红蛋白、3.2 g/dL的甘油三酯和100μg/mL头孢曲松对检测结果无干扰。结论极速热循环荧光定量PCR系统体积小、重量轻、无需核酸提取步骤、耗时短、检测性能良好,适用于即时检测(point-of-care testing,POCT)。
文摘Compassion is one of the core fundamental elements of Buddhism. The compassionate virtues are the precious qualities essential in the practice of Buddhism. On the other hand, guanxi plays a dominant role in any successful business venture in Chinese market. This paper develops the understanding of the two ancient bodies of knowledge of Buddhism and Confucianism. The attempt will be made in this paper to understand the deeper meaning of compassion in Buddhism in terms of compassionate virtues such as generosity, discipline, patience, diligence, humility, and wisdom. In guanxi, it will delve into different aspects of guanxi in terms of its background, meaning, significance, different types, intermediary, comparison with networking and ethics and success factors. This paper is based on the literature review on compassion--guanxi. The compassion literature focuses on books on Buddhism and commentaries by great Tibetan Buddhist sages. The guanxi literatures are predominately based on business research papers related to guanxi and business culture in China. The paper provides deeper meaning of compassion in Buddhism. The application of compassion to manage guanxi in China is the topic of this paper. This research is about optimizing the art of guanxi through the application of compassion which will help many non-Chinese business managers to effectively manage it. This paper is considered the first of its kind to study compassion in Buddhism and guanxi practice in China and seeks to provide the necessary framework to conduct the further research on applying compassion to build and manage better guanxi in China.
文摘This paper report the binding pattern of 12 different kinds of lectin in human breast lesions. Of the 12 kinds of lectins, WGA showed the highest binding activity to the cells of the breast tissue derived; the binding of BSL, SBA and DBA were localized to membrane or cytoplasm of cancer cells and to the lumina membrane border of the normal and benign lesions; PNA receptor is related with the differentiation of the breast; cancer; we didn't find any relationship between the lectin receptor and the tendency of metastasis of breast cancer.
文摘Eight lectins were used to study 100 cases of breast carcinoma and 56 cases of non-carcinoma breast tissues by lectin affinity histochemical method. The results showed that Bandeirasa Simplicifolia (BSL) and Peanut agglutinin (PNA) had higher positive rates in breast carcinoma than both normal breast and benign lesions (P<0.005). The positive deposit in malignant lesions was mainly located in cytoplasm, while in non-malignant lesions, it was almost lined along the lumen of glands and small ducts (P<0.005). The authors think that expression of PNA-receptor in the cytoplasm might be associated with the mechanism that the tumor could escape from immune attack. Comparison analysis on the normal breast indicated that PNA affinity histoche-mistry would be useful to the understanding of the metabolism of β-D-galactosyl-N-acetyl-D-galactosa-mine during the development of normal breast and histological origin of breast carcinoma.
