Objective To study the changes in serum small molecule metabolites after brucella infection in humans using untargeted metabolomics methods,and screening representative biomarkers.Methods A total of 109 serum samples ...Objective To study the changes in serum small molecule metabolites after brucella infection in humans using untargeted metabolomics methods,and screening representative biomarkers.Methods A total of 109 serum samples collected from January 2019 to December 2021 at the Brucellosis Clinic of the Baotou Center for Disease Control and Prevention were divided into acute phase group(n=40),chronic phase group(n=35)of brucellosis,and healthy group(n=34)based on clinical diagnosis.Ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry technology was used to test serum samples and screen for differential metabolites.Receiver operating characteristic curve was used to evaluate the predictive ability of differential metabolites for brucellosis.Enriched pathways were screened using Kyoto Encyclopedia of Genes and Genomes(KECG)pathway toidentifyymetabolic pathways significantly affected.Results A total of 17 differential metabolites were screened between the acute phase group and the healthy group,and 12 differential metabolites were screened between the chronic phase group and the healthy group.展开更多
Objective To observe the effect of acupuncture combined with infantile tuina on intestinal flora and its efficacy in children with tic disorders(TD),and to explore its mechanism.Methods A total of 15 childrenwith TD w...Objective To observe the effect of acupuncture combined with infantile tuina on intestinal flora and its efficacy in children with tic disorders(TD),and to explore its mechanism.Methods A total of 15 childrenwith TD were recruited as an observation group and 10healthy children as a healthy control group.展开更多
Objective To investigate the role of Keapl/Nrf2/HO-1 signaling pathway in liver injury induced by neodymium oxide(Nd_(2)O_(3))in mice.Methods In March 2021,forty-eight SPF grade healthy male C57BL/6J mice were randoml...Objective To investigate the role of Keapl/Nrf2/HO-1 signaling pathway in liver injury induced by neodymium oxide(Nd_(2)O_(3))in mice.Methods In March 2021,forty-eight SPF grade healthy male C57BL/6J mice were randomly divided into control group(0.9%NaCl).展开更多
Background Parkinson＇s disease （PD） is an autosomally inherited neurodegenerative disease in elderly people.The etiology of PD has long been thought to be associated with both genetic and environmental factors.To e...Background Parkinson＇s disease （PD） is an autosomally inherited neurodegenerative disease in elderly people.The etiology of PD has long been thought to be associated with both genetic and environmental factors.To explore potential genetic risk factors for PD in the northern Han Chinese population,we investigated three single nucleotide polymorphisms （SNPs） （rs4538475,rs11107 and rs12564040） in the BST1,PARK15 and PARK9 genes.Methods Genomic DNA from 215 PD patients and 212 matched controls was amplified in two independent PCR systems and subsequently genotyped by digestion with the endonuclease Pstl.Genetic parameter and association studies were carried out with SPSS 13.0 and PLINK 1.07 software.Results We could accurately detect all genotypes in the three loci with the PCR-RFLP or mismatched PCR-RFLP techniques.The observed heterozygosities of the rs4538475 and rs11107 loci in PD and control groups ranged from 0.460-0.481 and 0.410-0.441,in BST1,PARK15 respectively,while we detected no heterozygosity at the rs12564040 locus in PARK9.The similar distributions of genotypic frequency between both groups suggest that the three SNPs investigated in this study are unlikely to play roles as common risk factors or pathogenic mutations for PD in northern Han Chinese.Conclusion The SNPs investigated in the BST1,PARK15 and PARK9 genes associated with PD susceptibility are not associated with PD in the northern Han Chinese population.展开更多
文摘Objective To study the changes in serum small molecule metabolites after brucella infection in humans using untargeted metabolomics methods,and screening representative biomarkers.Methods A total of 109 serum samples collected from January 2019 to December 2021 at the Brucellosis Clinic of the Baotou Center for Disease Control and Prevention were divided into acute phase group(n=40),chronic phase group(n=35)of brucellosis,and healthy group(n=34)based on clinical diagnosis.Ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry technology was used to test serum samples and screen for differential metabolites.Receiver operating characteristic curve was used to evaluate the predictive ability of differential metabolites for brucellosis.Enriched pathways were screened using Kyoto Encyclopedia of Genes and Genomes(KECG)pathway toidentifyymetabolic pathways significantly affected.Results A total of 17 differential metabolites were screened between the acute phase group and the healthy group,and 12 differential metabolites were screened between the chronic phase group and the healthy group.
文摘Objective To observe the effect of acupuncture combined with infantile tuina on intestinal flora and its efficacy in children with tic disorders(TD),and to explore its mechanism.Methods A total of 15 childrenwith TD were recruited as an observation group and 10healthy children as a healthy control group.
文摘Objective To investigate the role of Keapl/Nrf2/HO-1 signaling pathway in liver injury induced by neodymium oxide(Nd_(2)O_(3))in mice.Methods In March 2021,forty-eight SPF grade healthy male C57BL/6J mice were randomly divided into control group(0.9%NaCl).
基金This work was supported by a grant from the National Natural Science Foundation of China （No.30771833）.
文摘Background Parkinson＇s disease （PD） is an autosomally inherited neurodegenerative disease in elderly people.The etiology of PD has long been thought to be associated with both genetic and environmental factors.To explore potential genetic risk factors for PD in the northern Han Chinese population,we investigated three single nucleotide polymorphisms （SNPs） （rs4538475,rs11107 and rs12564040） in the BST1,PARK15 and PARK9 genes.Methods Genomic DNA from 215 PD patients and 212 matched controls was amplified in two independent PCR systems and subsequently genotyped by digestion with the endonuclease Pstl.Genetic parameter and association studies were carried out with SPSS 13.0 and PLINK 1.07 software.Results We could accurately detect all genotypes in the three loci with the PCR-RFLP or mismatched PCR-RFLP techniques.The observed heterozygosities of the rs4538475 and rs11107 loci in PD and control groups ranged from 0.460-0.481 and 0.410-0.441,in BST1,PARK15 respectively,while we detected no heterozygosity at the rs12564040 locus in PARK9.The similar distributions of genotypic frequency between both groups suggest that the three SNPs investigated in this study are unlikely to play roles as common risk factors or pathogenic mutations for PD in northern Han Chinese.Conclusion The SNPs investigated in the BST1,PARK15 and PARK9 genes associated with PD susceptibility are not associated with PD in the northern Han Chinese population.
