Hepatocellular carcinoma(HCC)is the third leading cause of cancer death worldwide.Ginsenoside Rk3,an important and rare saponin in heat-treated ginseng,is generated from Rg1 and has a smaller molecular weight.However,...Hepatocellular carcinoma(HCC)is the third leading cause of cancer death worldwide.Ginsenoside Rk3,an important and rare saponin in heat-treated ginseng,is generated from Rg1 and has a smaller molecular weight.However,the anti-HCC efficacy and mechanisms of ginsenoside Rk3 have not yet been characterized.Here,we investigated the mechanism by which ginsenoside Rk3,a tetracyclic triterpenoid rare ginsenoside,inhibits the growth of HCC.We first explored the possible potential targets of Rk3 through network pharmacology.Both in vitro(HepG2 and HCC-LM3 cells)and in vivo(primary liver cancer mice and HCC-LM3 subcutaneous tumor-bearing mice)studies revealed that Rk3 significantly inhibits the proliferation of HCC.Meanwhile,Rk3 blocked the cell cycle in HCC at the G1 phase and induced autophagy and apoptosis in HCC.Further proteomics and siRNA experiments showed that Rk3 regulates the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway to inhibit HCC growth,which was validated by molecular docking and surface plasmon resonance.In conclusion,we report the discovery that ginsenoside Rk3 binds to PI3K/AKT and promotes autophagy and apoptosis in HCC.Our data strongly support the translation of ginsenoside Rk3 into novel PI3K/AKT-targeting therapeutics for HCC treatment with low toxic side effects.展开更多
Ginsenosides are the main pharmacologically active constituents of ginseng which have been used in East Asian countries for centuries to modulate blood pressure,metabolism and immune function.Following the technologic...Ginsenosides are the main pharmacologically active constituents of ginseng which have been used in East Asian countries for centuries to modulate blood pressure,metabolism and immune function.Following the technological advances in isolation,purification and mass production,their mechanisms of action are gradually elucidated,providing solid basis for clinical applications.Ginseng extracts(total ginsenosides)and ginsenoside Rg3,CK,Rd have been marketed or entered clinical trials as drugs or dietary supplements.Despite the proven safety and efficacy of some ginsenosides,their applications are hindered by inferior pharmacokinetics such as low solubility,poor membrane permeability and metabolic instability.Nanoparticle formulation of drugs and implantable drug depots are effective strategies to improve the pharmacokinetics of therapeutic agents by enhancing solubility,providing protection,facilitating intracellular transport,and enabling sustained and controlled release.This mini-review summarizes the recent advances in systemic delivery of ginsenosides using liposomes,micelles,albumin-based nanoparticles,and inorganic nanoparticles,as well as local delivery of ginsenosides by electronspun fibrous membranes and hydrogels.展开更多
The therapy of bone defects based on advanced biological scaffolds offers the most promising therapeutic strategy for bone reconstruction.It is still challenging to develop scaffolds with the mechanical,osteogenic,ang...The therapy of bone defects based on advanced biological scaffolds offers the most promising therapeutic strategy for bone reconstruction.It is still challenging to develop scaffolds with the mechanical,osteogenic,angiogenic,and antibacterial properties required for bone defect reconstruction.Here,a novel organic/inorganic composite scaffold(zinc-whitlockite(ZnWH)/G/H)was synthesized using gellan gum(GG),human-like collagen(HLC),and ZnWH nanoparticles.The scaffold had excellent mechanical properties,adjustable swelling ratio,and interconnected pore structure.In addition to its excellent biocompatibility,it could promote osteogenic differentiation by releasing ZnWH nanoparticles to stimulate human bone marrow mesenchymal stem cells(hBMSCs)to upregulate the levels of alkaline phosphatase(ALP),osteocalcin(OCN),and osteopontin(OPN).In addition,this study showed that ZnWH nanoparticles could also promote angiogenesis by upregulating the paracrine secretion of vascular endothelial growth factor(VEGF)in hBMSCs.At the same time,the scaffold could inhibit the proliferation of bacteria.After 12 weeks of treatment in the rabbit femoral defect model,the ZnWH/G/H scaffold significantly accelerated the process of bone reconstruction.Therefore,these results demonstrate that the prepared novel nanocomposite scaffold,ZnWH/G/H,offers a promising candidate for bone regeneration.展开更多
Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has crea...Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has created a new problem of superbugs.Herein,we propose a new strategy to obtain pH-responsive antimicrobial P-ZIF(ZIF:zeolitic imidazolate framework)by loading polyhexamethylenebiguanide(PHMB)into the framework of ZIF-8 nanoparticles.This will enable PHMB to be released in the weak acid environment of an infected wound.To address burn infections,P-ZIF nanoparticles were loaded into a hydrogel system made of sodium alginate(SA)and 3-aminophenylboronic acid modified human-like collagen(H-A)through borate ester bonds.The resulting H-A/SA/P-ZIF(HASPZ)hydrogel dressing not only possesses antibacterial and wound healing properties but also has dual pH responsiveness to prevent the overuse of medication while effectively treat deep second-degree burns.Therefore,P-ZIF nanoparticles and the corresponding HASPZ hydrogel dressing are considered of significant importance in antimicrobial,drug delivery,and wound repair.展开更多
Bacterial infection causes wound inflammation and makes angiogenesis difficult.It is urgent to develop effectively antibacterial and pro-vascularizing dressings for wound healing.The hydrogel is developed with pH-resp...Bacterial infection causes wound inflammation and makes angiogenesis difficult.It is urgent to develop effectively antibacterial and pro-vascularizing dressings for wound healing.The hydrogel is developed with pH-responsive drug-releasing microcarriers which were loaded with vascular endothelial growth factor(VEGF)that promotes angiogenesis and actively respond to wound pH for control and prolong VEGF release.The surfaces of the microcarriers were coated with polydopamine which can reduce the silver nanoparticles(AgNPs)in situ,and dynamically crosslink with the polyacrylamide,which forms a stable slow-release system with different release behavior for the VEGF and AgNPs.The hydrogel inhib-ited bacterial formation and accelerated wound healing.With the hydrogel dressing,83.3%±4.29%of the wound heals at day 7,which is 40.9%±8.5%higher than the non-treatment group in defect infected model.The antibacterial properties of hydrogel down-regulate early inflammation-related cytokines,and the release of VEGF in the middle and late phases of wound healing in response to pH changes pro-motes angiogenesis and up-regulate the expression of angiogenesis-associated cytokine.The sequential release of antibacterial agents and pro-vascularizing agents in response to the change in wound microen-vironmental cues facilitate temporally controlled therapy that suites the need of different wound healing phases.Collectively,the hydrogel loaded with multifunctional microcarriers that enable controlled release of AgNPs and VEGF is an effective system for treating infected wounds.展开更多
The repair and treatment of tumor bone defects is a difficult problem to solve urgently in clinical medicine.After tumor resection,patients are not only faced with a large area of bone defect,but also may have the ris...The repair and treatment of tumor bone defects is a difficult problem to solve urgently in clinical medicine.After tumor resection,patients are not only faced with a large area of bone defect,but also may have the risk of tumor recurrence,which can easily cause huge physical and mental harm to patients.In this study,we successfully designed and constructed an organic/inorganic composite microgel bone powder(S-H-M3%Ce/3%Se)based on cerium(Ce)and selenium(Se)elements co-doped mesoporous bioactive glass(M3%Ce/3%Se),sodium alginate(SA),and recombinant human-like collagen(HLC).The obtained S-H-M3%Ce/3%Se could inhibit the growth of osteoma cells and promote the growth of normal cells,and effectively promote the repair of defect bone.The integration of the“treatment and repair”organic/inorganic composite microgel bone powder provided a new strategy for the treatment of cancerous bone defects.展开更多
Chronic diabetic wounds are an important healthcare challenge. High concentration glucose, high level of matrix metalloproteinase-9 (MMP-9), and long-term inflammation constitute the special wound environment of diabe...Chronic diabetic wounds are an important healthcare challenge. High concentration glucose, high level of matrix metalloproteinase-9 (MMP-9), and long-term inflammation constitute the special wound environment of diabetic wounds. Tissue necrosis aggravates the formation of irregular wounds. All the above factors hinder the healing of chronic diabetic wounds. To solve these issues, a glucose and MMP-9 dual-response temperature-sensitive shape self-adaptive hydrogel (CBP/GMs@Cel&INS) was designed and constructed with polyvinyl alcohol (PVA) and chitosan grafted with phenylboric acid (CS-BA) by encapsulating insulin (INS) and gelatin microspheres con-taining celecoxib (GMs@Cel). Temperature-sensitive self-adaptive CBP/GMs@Cel&INS provides a new way to balance the fluid-like mobility (self-adapt to deep wounds quickly, approximately 37 ◦C) and solid-like elasticity (protect wounds against external forces, approximately 25 ◦C) of self-adaptive hydrogels, while simultaneously releasing insulin and celecoxib on-demand in the environment of high-level glucose and MMP-9. Moreover, CBP/ GMs@Cel&INS exhibits remodeling and self-healing properties, enhanced adhesion strength (39.65 ± 6.58 kPa), down-regulates MMP-9, and promotes cell proliferation, migration, and glucose consumption. In diabetic full-thickness skin defect models, CBP/GMs@Cel&INS significantly alleviates inflammation and regulates the local high-level glucose and MMP-9 in the wounds, and promotes wound healing effectively through the synergistic effect of temperature-sensitive shape-adaptive character and the dual-responsive system.展开更多
Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)w...Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing.展开更多
Flexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis.Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts...Flexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis.Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts,but arising problems such as skin irritation,rubefaction,and pain upon device removal have hampered their utility.Here,inspired by the temperature reversibility of hydrogen bonding,a skin-friendly conductive hydrogel with multiple-hydrogen bonds was designed by using biocompatible poly(vinyl alcohol)(PVA),phytic acid(PA),and gelatin(Gel).The obtained PVA/PA/Gel(PPG)hydrogel with temperature-triggered tunable mechanic could reliably adhere to skin and detect electrophysiological signals under a hot compress while be readily removed under a cool compress.Furthermore,the additional advantages of transparency,breathability,and antimicrobial activity of the PPG hydrogel ensure its long-time wearable value on the skin.It is both environmentally friendly and cost saving for the waste PPG hydrogel during production can be recycled based on their reversible physical bonding.The PPG hydrogel sensor is expected to have good application prospects to record electrophysiological signals in human health monitoring.展开更多
Solar dermatitis is an acute or chronic high incidence of skin injury caused by ultraviolet(UV)radiation based on strong sunlight,which seriously endangers people's health.In this study,we designed and demonstrate...Solar dermatitis is an acute or chronic high incidence of skin injury caused by ultraviolet(UV)radiation based on strong sunlight,which seriously endangers people's health.In this study,we designed and demonstrated enzyme-catalyzed semi-inter penetrating polymer network(Semi-IPN)sprayable nanodrug-loaded hydrogels based on gelatin,3-(4-hydroxyphenyl)propionic acid(HPA),polyvinyl alcohol(PVA),glycerol,and dexamethasone sodium phosphate(DEXP)for solar dermatitis.The hydrogels had high water content,excellent biocompatibility,effective encapsulation and sustained release of nanodrugs,antiinflammatory,and strong anti-ultraviolet B(anti-UVB)radiation properties based on glycerol and phenol functional groups,but also controllable spray gelation mode to make them adhere well on the dynamic skin surfaces and achieve continuous transdermal drugs delivery for solar dermatitis.The sprayable nanodrug-loaded hydrogel systems could be used as a highly effective therapeutic method for solar dermatitis,and also provide a good strategy for designing novel nanodrug-loaded hydrogel delivery systems.展开更多
基金supported by the National Key R&D Program of China(Grant No.:2021YFC2101500)the National Natural Science Foundation of China(Grant Nos.:22078264,21978235,22108224,and 21978236)+2 种基金the Natural Science Basic Research Program of Shaanxi,China(Grant Nos.:2023-JC-JQ-17 and 2023-JCQN-0109)the Xi'an Science and Technology Project,China(Project No.:20191422315KYPT014JC016)Key Research and Development Program of Shaanxi,China(Grant No.:2022ZDLSF05-12).
文摘Hepatocellular carcinoma(HCC)is the third leading cause of cancer death worldwide.Ginsenoside Rk3,an important and rare saponin in heat-treated ginseng,is generated from Rg1 and has a smaller molecular weight.However,the anti-HCC efficacy and mechanisms of ginsenoside Rk3 have not yet been characterized.Here,we investigated the mechanism by which ginsenoside Rk3,a tetracyclic triterpenoid rare ginsenoside,inhibits the growth of HCC.We first explored the possible potential targets of Rk3 through network pharmacology.Both in vitro(HepG2 and HCC-LM3 cells)and in vivo(primary liver cancer mice and HCC-LM3 subcutaneous tumor-bearing mice)studies revealed that Rk3 significantly inhibits the proliferation of HCC.Meanwhile,Rk3 blocked the cell cycle in HCC at the G1 phase and induced autophagy and apoptosis in HCC.Further proteomics and siRNA experiments showed that Rk3 regulates the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway to inhibit HCC growth,which was validated by molecular docking and surface plasmon resonance.In conclusion,we report the discovery that ginsenoside Rk3 binds to PI3K/AKT and promotes autophagy and apoptosis in HCC.Our data strongly support the translation of ginsenoside Rk3 into novel PI3K/AKT-targeting therapeutics for HCC treatment with low toxic side effects.
基金This work was financially supported by the National Natural Science Foundation of China(Grant Nos.22078264,21978235,21776227 and 21706211)the Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2019JQ259)Northwest Northwest University Graduate Innovation Project(Grant No.YZZ17128).
文摘Ginsenosides are the main pharmacologically active constituents of ginseng which have been used in East Asian countries for centuries to modulate blood pressure,metabolism and immune function.Following the technological advances in isolation,purification and mass production,their mechanisms of action are gradually elucidated,providing solid basis for clinical applications.Ginseng extracts(total ginsenosides)and ginsenoside Rg3,CK,Rd have been marketed or entered clinical trials as drugs or dietary supplements.Despite the proven safety and efficacy of some ginsenosides,their applications are hindered by inferior pharmacokinetics such as low solubility,poor membrane permeability and metabolic instability.Nanoparticle formulation of drugs and implantable drug depots are effective strategies to improve the pharmacokinetics of therapeutic agents by enhancing solubility,providing protection,facilitating intracellular transport,and enabling sustained and controlled release.This mini-review summarizes the recent advances in systemic delivery of ginsenosides using liposomes,micelles,albumin-based nanoparticles,and inorganic nanoparticles,as well as local delivery of ginsenosides by electronspun fibrous membranes and hydrogels.
基金This work was supported by the National Natural Science Foundation of China(Nos.21908179 and 22008196)the National Key Research and Development Program of China(2021YFC2103900)the Natural Science Foundation of Shaanxi Province,China(No.218JQ2052).
文摘The therapy of bone defects based on advanced biological scaffolds offers the most promising therapeutic strategy for bone reconstruction.It is still challenging to develop scaffolds with the mechanical,osteogenic,angiogenic,and antibacterial properties required for bone defect reconstruction.Here,a novel organic/inorganic composite scaffold(zinc-whitlockite(ZnWH)/G/H)was synthesized using gellan gum(GG),human-like collagen(HLC),and ZnWH nanoparticles.The scaffold had excellent mechanical properties,adjustable swelling ratio,and interconnected pore structure.In addition to its excellent biocompatibility,it could promote osteogenic differentiation by releasing ZnWH nanoparticles to stimulate human bone marrow mesenchymal stem cells(hBMSCs)to upregulate the levels of alkaline phosphatase(ALP),osteocalcin(OCN),and osteopontin(OPN).In addition,this study showed that ZnWH nanoparticles could also promote angiogenesis by upregulating the paracrine secretion of vascular endothelial growth factor(VEGF)in hBMSCs.At the same time,the scaffold could inhibit the proliferation of bacteria.After 12 weeks of treatment in the rabbit femoral defect model,the ZnWH/G/H scaffold significantly accelerated the process of bone reconstruction.Therefore,these results demonstrate that the prepared novel nanocomposite scaffold,ZnWH/G/H,offers a promising candidate for bone regeneration.
基金supported by the National key Research and Development Program of China(Nos.2021YFC2101504,2021YFC2103900,and 2019YFA0905200)the National Natural Science Foundation of China(Nos.22078265 and 21908179)the Natural Science Foundation of Shaanxi Province,China(No.218JQ2052).
文摘Burns are a common medical problem globally,and wound infection is one of the major causes of inducing related complications.Although antibiotics effectively prevent wound infections,the misuse of antibiotics has created a new problem of superbugs.Herein,we propose a new strategy to obtain pH-responsive antimicrobial P-ZIF(ZIF:zeolitic imidazolate framework)by loading polyhexamethylenebiguanide(PHMB)into the framework of ZIF-8 nanoparticles.This will enable PHMB to be released in the weak acid environment of an infected wound.To address burn infections,P-ZIF nanoparticles were loaded into a hydrogel system made of sodium alginate(SA)and 3-aminophenylboronic acid modified human-like collagen(H-A)through borate ester bonds.The resulting H-A/SA/P-ZIF(HASPZ)hydrogel dressing not only possesses antibacterial and wound healing properties but also has dual pH responsiveness to prevent the overuse of medication while effectively treat deep second-degree burns.Therefore,P-ZIF nanoparticles and the corresponding HASPZ hydrogel dressing are considered of significant importance in antimicrobial,drug delivery,and wound repair.
文摘Bacterial infection causes wound inflammation and makes angiogenesis difficult.It is urgent to develop effectively antibacterial and pro-vascularizing dressings for wound healing.The hydrogel is developed with pH-responsive drug-releasing microcarriers which were loaded with vascular endothelial growth factor(VEGF)that promotes angiogenesis and actively respond to wound pH for control and prolong VEGF release.The surfaces of the microcarriers were coated with polydopamine which can reduce the silver nanoparticles(AgNPs)in situ,and dynamically crosslink with the polyacrylamide,which forms a stable slow-release system with different release behavior for the VEGF and AgNPs.The hydrogel inhib-ited bacterial formation and accelerated wound healing.With the hydrogel dressing,83.3%±4.29%of the wound heals at day 7,which is 40.9%±8.5%higher than the non-treatment group in defect infected model.The antibacterial properties of hydrogel down-regulate early inflammation-related cytokines,and the release of VEGF in the middle and late phases of wound healing in response to pH changes pro-motes angiogenesis and up-regulate the expression of angiogenesis-associated cytokine.The sequential release of antibacterial agents and pro-vascularizing agents in response to the change in wound microen-vironmental cues facilitate temporally controlled therapy that suites the need of different wound healing phases.Collectively,the hydrogel loaded with multifunctional microcarriers that enable controlled release of AgNPs and VEGF is an effective system for treating infected wounds.
基金This work was supported by the National Natural Science Foundation of China(Nos.22078265,21908179,and 21838009)the National key Research and development program of China(No.2021YFC2103900)the Shaanxi Provincial Science Foundation(Nos.2017SF-201 and 218JQ2052).
文摘The repair and treatment of tumor bone defects is a difficult problem to solve urgently in clinical medicine.After tumor resection,patients are not only faced with a large area of bone defect,but also may have the risk of tumor recurrence,which can easily cause huge physical and mental harm to patients.In this study,we successfully designed and constructed an organic/inorganic composite microgel bone powder(S-H-M3%Ce/3%Se)based on cerium(Ce)and selenium(Se)elements co-doped mesoporous bioactive glass(M3%Ce/3%Se),sodium alginate(SA),and recombinant human-like collagen(HLC).The obtained S-H-M3%Ce/3%Se could inhibit the growth of osteoma cells and promote the growth of normal cells,and effectively promote the repair of defect bone.The integration of the“treatment and repair”organic/inorganic composite microgel bone powder provided a new strategy for the treatment of cancerous bone defects.
基金This work was supported by the National key Research and devel-opment program of China(2019YFA0905200)the National Natural Science Foundation of China(21878247,21808184)Key Program of the National Natural Science Foundation of China(21838009).
文摘Chronic diabetic wounds are an important healthcare challenge. High concentration glucose, high level of matrix metalloproteinase-9 (MMP-9), and long-term inflammation constitute the special wound environment of diabetic wounds. Tissue necrosis aggravates the formation of irregular wounds. All the above factors hinder the healing of chronic diabetic wounds. To solve these issues, a glucose and MMP-9 dual-response temperature-sensitive shape self-adaptive hydrogel (CBP/GMs@Cel&INS) was designed and constructed with polyvinyl alcohol (PVA) and chitosan grafted with phenylboric acid (CS-BA) by encapsulating insulin (INS) and gelatin microspheres con-taining celecoxib (GMs@Cel). Temperature-sensitive self-adaptive CBP/GMs@Cel&INS provides a new way to balance the fluid-like mobility (self-adapt to deep wounds quickly, approximately 37 ◦C) and solid-like elasticity (protect wounds against external forces, approximately 25 ◦C) of self-adaptive hydrogels, while simultaneously releasing insulin and celecoxib on-demand in the environment of high-level glucose and MMP-9. Moreover, CBP/ GMs@Cel&INS exhibits remodeling and self-healing properties, enhanced adhesion strength (39.65 ± 6.58 kPa), down-regulates MMP-9, and promotes cell proliferation, migration, and glucose consumption. In diabetic full-thickness skin defect models, CBP/GMs@Cel&INS significantly alleviates inflammation and regulates the local high-level glucose and MMP-9 in the wounds, and promotes wound healing effectively through the synergistic effect of temperature-sensitive shape-adaptive character and the dual-responsive system.
基金This work was supported by the National Natural Science Foundation of China(grant numbers 21878247)Key Program of the National Natural Science Foundation of China(grant numbers 21838009)+2 种基金National Key Research and Development Program(2019YFA0905200)and Xi’an Science and Technology Project(20191422315KYPT014JC016)The authors thank Dr.J.C.Li(School of Chemical Engineering,Northwest University,Xi’an,China)for improving the manuscript during revising.
文摘Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing.
基金supported by the National Key R&D Program of China (2021YFB3200302)the National Natural Science Foundation of China (Nos.22078265,21838009,22075139,62101545,and 21908179)by the Shaanxi Provincial Science Foundation (Nos.2017SF-201).
文摘Flexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis.Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts,but arising problems such as skin irritation,rubefaction,and pain upon device removal have hampered their utility.Here,inspired by the temperature reversibility of hydrogen bonding,a skin-friendly conductive hydrogel with multiple-hydrogen bonds was designed by using biocompatible poly(vinyl alcohol)(PVA),phytic acid(PA),and gelatin(Gel).The obtained PVA/PA/Gel(PPG)hydrogel with temperature-triggered tunable mechanic could reliably adhere to skin and detect electrophysiological signals under a hot compress while be readily removed under a cool compress.Furthermore,the additional advantages of transparency,breathability,and antimicrobial activity of the PPG hydrogel ensure its long-time wearable value on the skin.It is both environmentally friendly and cost saving for the waste PPG hydrogel during production can be recycled based on their reversible physical bonding.The PPG hydrogel sensor is expected to have good application prospects to record electrophysiological signals in human health monitoring.
基金supported by the National Natural Science Foundation of China(Nos.22078265,22108225,and 21838009)the Shaanxi Provincial Science Foundation(Nos.2017SF-201 and 2021JQ-434)China Postdoctoral Science Foundation(No.2018M643720).
文摘Solar dermatitis is an acute or chronic high incidence of skin injury caused by ultraviolet(UV)radiation based on strong sunlight,which seriously endangers people's health.In this study,we designed and demonstrated enzyme-catalyzed semi-inter penetrating polymer network(Semi-IPN)sprayable nanodrug-loaded hydrogels based on gelatin,3-(4-hydroxyphenyl)propionic acid(HPA),polyvinyl alcohol(PVA),glycerol,and dexamethasone sodium phosphate(DEXP)for solar dermatitis.The hydrogels had high water content,excellent biocompatibility,effective encapsulation and sustained release of nanodrugs,antiinflammatory,and strong anti-ultraviolet B(anti-UVB)radiation properties based on glycerol and phenol functional groups,but also controllable spray gelation mode to make them adhere well on the dynamic skin surfaces and achieve continuous transdermal drugs delivery for solar dermatitis.The sprayable nanodrug-loaded hydrogel systems could be used as a highly effective therapeutic method for solar dermatitis,and also provide a good strategy for designing novel nanodrug-loaded hydrogel delivery systems.
