BACKGROUND: The indocyanine green (ICG) clearance test (clearance rate (K) and retention rate at 15 minutes (R(15))) is a sensitive indicator to evaluate liver function. The model for end-stage liver disease (MELD) sc...BACKGROUND: The indocyanine green (ICG) clearance test (clearance rate (K) and retention rate at 15 minutes (R(15))) is a sensitive indicator to evaluate liver function. The model for end-stage liver disease (MELD) score has emerged as a useful tool for estimating the mortality of patients awaiting liver transplantation and has recently been validated on patients with liver diseases of various etiologies and severity. In this study, we investigated the correlation between the ICG clearance test and MELD score of patients with liver cirrhosis. METHODS: From June 2007 to March 2008, 52 patients with liver cirrhosis admitted to our center were classified into Child-Pugh class A (8 patients), B (14) and C (30). The ICG clearance test (K value and R(15)) was performed by ICG pulse spectrophotometry (DDG-3300K), and the MELD scores of patients were calculated. RESULTS: As the Child-Pugh classification of liver function gradually deteriorated, the K value decreased, while R(15) and MELD score increased. There were significant statistical differences in K value, R(15) and MELD score in patients with different Child-Pugh classifications. Significant correlations were found between the parameters of the ICG clearance test (K value and R(15)) and MELD score. A negative correlation was observed between K value and MELD score (r=-0.892, P < 0.05), while a positive correlation was observed between R(15) and MELD score (r=0.804, P < 0.05). CONCLUSIONS: The ICG clearance test and MELD score are good parameters for evaluating liver function. Moreover, K value and R(15) have significant correlations with MELD score, especially the K value, which may be a convenient and appropriate indicator to evaluate liver function of patients with liver cirrhosis.展开更多
AIM:To study characteristics of collateral circulation of gastric varices (GVs) with 64-row multidetector computer tomography portal venography (MDCTPV).METHODS:64-row MDCTPV with a slice thickness of 0.625 mm and a s...AIM:To study characteristics of collateral circulation of gastric varices (GVs) with 64-row multidetector computer tomography portal venography (MDCTPV).METHODS:64-row MDCTPV with a slice thickness of 0.625 mm and a scanning field from 2 cm above the tracheal bifurcation to the lower edge of the kidney was performed in 86 patients with GVS diagnosed by endoscopy. The computed tomography protocol included unenhanced,arterial and portal vein phases. The MDCTPV was performed on an AW4.3 workstation. GVs were classified into three types according to Sarin's Classification. The afferent and efferent veins of each type of GV were observed.RESULTS:The afferent venous drainage originated mostly from the left gastric vein alone (LGV) (28/86,32.59%),or the LGV more than the posterior gastric vein/short gastric vein [LGV > posterior gastric vein/short gastric vein (PGV/SGV)] (22/86,25.58%),as seen by MDCTPV. The most common efferent venousdrainage was via the azygos vein to the superior vena cava (53/86,61.63%),or via the gastric/splenorenal shunt (37/86,43.02%) or inferior phrenic vein (8/86,9.30%) to the inferior vena cava. In patients with gastroesophageal varices type 1,the afferent venous drainage of GV mainly originated from the LGV or LGV > PGV/SGV (43/48,89.58%),and the efferent venous drainage was mainly via the azygos vein to the super vena cava (43/48,89.58%),as well as via the gastric/splenorenal shunt (8/48,16.67%) or inferior phrenic vein (3/48,6.25%) to the inferior vena cava. In patients with gastroesophageal varices type 2,the afferent venous drainage of the GV mostly came from the PGV/SGV more than the LGV (PGV/SGV > LGV) (8/16,50%),and the efferent venous drainage was via the azygos vein (10/16,62.50%) and gastric/splenorenal shunt (9/16,56.25%). In patients with isolated gastric varices,the main afferent venous drainage was via the PGV/SGV alone (16/22,72.73%),and the efferent venous drainage was mainly via the gastric/splenorenal shunt (20/22,90.91%),as well as the inferior phrenic vein (3/23) to the inferior vena cava. CONCLUSION:MDCTPV can clearly display the afferent and efferent veins of all types of GV,and it could provide useful reference information for the clinical management of GV bleeding.展开更多
BACKGROUND: Autoimmune pancreatitis (All?) is a unique chronic inflammation of the pancreas in which autoimmune mechanisms are involved in the pathogenesis. It is characterized by clinical, histopathological, radiogra...BACKGROUND: Autoimmune pancreatitis (All?) is a unique chronic inflammation of the pancreas in which autoimmune mechanisms are involved in the pathogenesis. It is characterized by clinical, histopathological, radiographic, serologic and therapeutic features. Since it was first described in 1995, increasing numbers of AIP patients have been diagnosed. METHODS: In this study, the data from 2 patients with AIP who had elevation of serum immunoglobulin G, positive autoantibody, swelling of the pancreas and narrowing of the main pancreatic duct on imaging were analyzed retrospectively. RESULTS: With the initial diagnosis of AIP, both patients underwent regular prednisone therapy, which was initiated at a dose of 40 mg per day with a tapering schedule of 5 mg every 2 weeks. The patients responded very well to treatment with prednisone. CONCLUSIONS: AIP is a relatively new disease entity. Although it is diagnosed by distinct characteristics of the clinical, radiologic, histopathologic, and serologic findings, many questions require further clarification, including its relationship to other autoimmune diseases and misdiagnosis as pancreaticobiliary malignancies.展开更多
BACKGROUND: Triptolide (TPT) is a diterpenoid triepoxide extracted from the Chinese herb Tripterygium wilfordii Hook. F. It exhibits potent immunosuppressive and anti-inflammatory properties. This study was undertaken...BACKGROUND: Triptolide (TPT) is a diterpenoid triepoxide extracted from the Chinese herb Tripterygium wilfordii Hook. F. It exhibits potent immunosuppressive and anti-inflammatory properties. This study was undertaken to investigate its effects on prolongation of islet allograft survival in rodents. Additionally, we investigated whether TPT would be toxic to islet function in vivo. METHODS: We transplanted BALB/c islets to either chemically induced diabetic C57BL/6 mice or spontaneously diabetic non-obese diabetic (NOD) mice. TPT was injected within 2 weeks or continuously, until rejection, in the two combinations. Then, we evaluated the toxicity of TPT on islet function by daily injection to naive BALB/c or diabetic BALB/c that was cured by syngeneic islet transplantation under the kidney capsule. Mice injected with cyclosporine A (CsA) or vehicle served as controls. Intraperitoneal glucose tolerance tests (IPGTTs) performed at 4 and 8 weeks in the naive BALB/c group, and at 2, 4, 6, and 8 weeks in the syngeneic transplanted group. RESULTS: The medium survival time of islets allograft from TPT treated C57BL/6 and NOD recipients were 28.5 days (range 24-30 days, n=10) and 33.0 days (range 15-47 days, n=6), respectively, and they were significantly different from those of the vehicle treated controls, which were 14.0 days (range 13-16 days, n=6) and 5.0 days (range 4-10 days, n=6), respectively (all P<0.0001). The IPGTT demonstrated that there was no difference between the TPT treated and vehicle treated groups, either in the normal or syngeneic transplanted islet BALB/c mice. However, CsA injection impaired islet function in both normal and syngeneic transplanted mice as early as 4 weeks. CONCLUSION: TPT prolonged islets allograft survival in a chemically induced diabetic or an autoimmune diabetic murine model without impairment of islet function. (Hepatobiliary Pancreat Dis Int 2010; 9: 312-318)展开更多
Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus. However, the effects of senegenin on mesencephalon-deriv...Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus. However, the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood. Cells from a ventral mesencephalon neural progenitor cell line （ReNcell VM） were utilized as models for pharmaceutical screening. The effects of various senegenin concentrations on cell proliferation were analyzed, demonstrating that high senegenin concentrations （5, 10, 50, and 100 μmol/L）, particularly 50 μmol/L, significantly promoted proliferation of ReNcell VM cells. In the mitogen-activated protein kinase signal transduction pathway, senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases. Moreover, cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors. Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase.展开更多
BACKGROUND: Interleukin-24 (IL-24) is a novel candidate tumor suppressor that induces tumor cell apoptosis experimentally in a variety of human malignant cells including liver cancer cells. The present study was condu...BACKGROUND: Interleukin-24 (IL-24) is a novel candidate tumor suppressor that induces tumor cell apoptosis experimentally in a variety of human malignant cells including liver cancer cells. The present study was conducted to investigate the potential effect of recombinant adeno-associated virus (rAAV)-mediated IL-24 gene therapy on tumor recurrence and metastasis by inducing tumor cell apoptosis in a hepatocellular carcinoma (HCC) model in nude mice. METHODS: We established a recurrent and metastatic HCC model in nude mice and constructed an rAAV vector carrying alpha-fetoprotein (AFP) promoter for expressing the IL-24 gene (rAVV/AFP/IL-24). The vector was administered by regional injection (liver incisal margin). AFP was detected by radiation immunoassay. Histological evaluation of tumor recurrence and metastasis was performed for the liver and lung. The effect of tumor cell apoptosis was confirmed by TUNEL analysis. RESULTS: IL-24 gene therapy prevented tumor recurrence and metastasis, as evidenced by marked decreases in the number of metastatic tumor nodules and tumor volume in the liver and lung. At the same time, serum AFP concentration decreased markedly in the IL-24 group compared with the control or rAAV groups (P<0.05). IL-24 gene therapy inhibited tumor recurrence and metastasis as evidenced by the induction of tumor cell apoptosis. CONCLUSION: The results demonstrated that targeted IL-24 gene therapy was effective in the prevention of postoperative recurrence and metastasis in an HCC nude mice model by induction of tumor cells apoptosis with potential minimum tumor burden.展开更多
BACKGROUND: The ischemic-type biliary lesion (ITBL) is one of the most serious biliary complications of liver transplantation. This study aimed to investigate the effects of autologous bone marrow mononuclear cell (BM...BACKGROUND: The ischemic-type biliary lesion (ITBL) is one of the most serious biliary complications of liver transplantation. This study aimed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation on neovascularization and the prevention of intrahepatic ITBL in a rabbit model. METHODS: The rabbits were divided into control, experimental model, and cell implantation groups, with 10 in each group. The model of intrahepatic ITBL was established by clamping the hepatic artery and common bile duct. Autologous BM-MNCs were isolated from the tibial plateau by density gradient centrifugation and were implanted through the common hepatic artery. Changes in such biochemical markers as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyltranspeptidase, total bilirubin and direct bilirubin were measured. Four weeks after operation, cholangiography, histopathological manifestations, differentiation of BM-MNCs, microvessel density and the expression of vascular endothelial growth factor were assessed. RESULTS: Compared with the experimental model group, the BM-MNC implantation group showed superiority in the time to recover normal biochemistry. The microvessel density and vascular endothelial growth factor expression of the implantation group were significantly higher than those of the control and experimental model groups. The ITBL in the experimental model group was more severe than that in the implantation group and fewer new capillary blood vessels occurred around it. CONCLUSIONS: Implanted autologous BM-MNCs can differentiate into vascular endothelial cells, promote neovascularization and improve the blood supply to the ischemic bile duct, and this provides a new way to diminish or prevent intrahepatic ITBL after liver transplantation. (Hepatobiliary Pancreat Dis Int 2010; 9:593-599)展开更多
Superwarfarins are a class of rodenticides. Gastrointestinal hemorrhage is a fatal complication of superwarfarin poisoning, requiring immediate treatment. Here, we report a 55-year-old woman with tardive upper gastroi...Superwarfarins are a class of rodenticides. Gastrointestinal hemorrhage is a fatal complication of superwarfarin poisoning, requiring immediate treatment. Here, we report a 55-year-old woman with tardive upper gastrointestinal hemorrhage caused by superwarfarin poisoning after endoscopic cold mucosal biopsy.展开更多
AIM:To investigate the eff icacy of acellular dermal matrix(ADM) for intestinal elongation in animal models.METHODS:Japanese white big-ear rabbits(n = 9) and Wuzhishan miniature pigs(n = 5) were used in the study.Home...AIM:To investigate the eff icacy of acellular dermal matrix(ADM) for intestinal elongation in animal models.METHODS:Japanese white big-ear rabbits(n = 9) and Wuzhishan miniature pigs(n = 5) were used in the study.Home-made and commercial ADM materials were used as grafts,respectively.A 3-cm long graft was interposed in continuity with the small bowel and a sideto-side anastomosis,distal to the graft about 3-4 cm,was performed.The animals were sacrificed at 2 wk,4 wk,8 wk and 3 mo after surgery and the histological changes were evaluated under light microscope and electron microscope.RESULTS:The animals survived after the operation with no evidence of peritonitis and sepsis.Severe ad-hesions were found between the graft and surrounding intestine.The grafts were completely absorbed within postoper ative two or three months except one.Histological observ ation showed inflammation in the grafts with fibrinoid necroses,infiltration of a large amount of neutrophils and leukomonocytes,and the degree varied in different stages.The neointestine with wellformed structures was not observed in the study.CONCLUSION:It is not suitable to use acellular dermal matrix alone as a scaffold for the intestinal elongation in animal models.展开更多
Objective To analyze the disease characteristics of aspiration pneumonia and its risk factors.Methods In thisretrospective case-control study,analysis was conducted on data from 92 patients aged≥60 years admitted to ...Objective To analyze the disease characteristics of aspiration pneumonia and its risk factors.Methods In thisretrospective case-control study,analysis was conducted on data from 92 patients aged≥60 years admitted to Beijing Jishuitan Hospital,Capital Medical University between June 1,2018 and July 31,2022,with aspiration pneumonia(AsP)as the primary diagnosis at the time of hospital discharge and from non-AsP patients admitted during the same period.The number of participants was matched at a 1:1 ratio.Results The average age of the AsP group was(80.88±9.41)years and 57(62.0%)were men.The average age of the control group was(77.74±10.98)years and 52(56.5%)were men.There was no statistically significant difference in age and sex ratio(age:t=1.973,P=0.060;sex ratio:X^(2)=0.661,P=0.416).Univariate analysis showed that,at admission,body mass index(BMI)and activities of daily living(ADL)scores of the AsP group were lower than those of the non-AsP group.展开更多
Objective To examine the clinical subtypes of patients with multisystem atrophy(MSA)that may indicate the prognosis of patients.Additionally,we aim to compare the ability to perform daily activities among patients of ...Objective To examine the clinical subtypes of patients with multisystem atrophy(MSA)that may indicate the prognosis of patients.Additionally,we aim to compare the ability to perform daily activities among patients of each subtype using cluster analysis.Methods The retrospective analysis included demographic data,clinical symptoms and signs,scale scores,and ancillary examinations of 94 patients diagnosed with multisystem atrophyatXuanwu Hospital of Capital Medical University.The study aimed to analyze the clinical characteristicssofeachhsubtype obtained through clustering.Additionally,acomparison was'made between patients with traditional motor subtypes and those with new subtypes in terms of activities of daily living.The study consisted of 94 MSA patients,with an average age of 61 years and a female representation of 51.1%.Using the data collected on the continuum,a full linkage hierarchical cluster analysis was performed to classify MSA patients into four clinical subtypes:gait disorder(17 cases,18.1%),malignant tonic hyperkinetic with premature haircut(25 cases,26.6%),intermediate(43 cases,45.7%)。展开更多
Objective To investigate the association between body mass index (BMI) and waist circumference (WC)with all-cause mortality in middle-aged and elderly patients receiving maintenance hemodialysis (MHD).Methods The clin...Objective To investigate the association between body mass index (BMI) and waist circumference (WC)with all-cause mortality in middle-aged and elderly patients receiving maintenance hemodialysis (MHD).Methods The clinical data of MHD patients aged≥50years old from eleven hemodialysis centers from April to June 2017 in Beijing were analyzed.展开更多
Objective To analyze the diagnostic efficacy of urinary lipoarabinomannan (LAM) antigen detection method in tuberculosis patients,and to provide an experimental basis for the clinical application of urinary LAM kit in...Objective To analyze the diagnostic efficacy of urinary lipoarabinomannan (LAM) antigen detection method in tuberculosis patients,and to provide an experimental basis for the clinical application of urinary LAM kit in China.Methods From March to May 2023,228 patients with lung diseases [134 male,94 female,age 20-82 (44.8±16.7) years] were prospectively collected in Beijing Chest Hospital,Capital Medical University,including 143 pulmonary tuberculosis patients and 85 non-tuberculosis patients.展开更多
Objective To investigate the in-hospital incidence,associated outcomes,and predictors of UGIB after STEMI.Methods In this retrospective study,we analyzed the records of 2 791 patients with acute STEMI admitted to Beij...Objective To investigate the in-hospital incidence,associated outcomes,and predictors of UGIB after STEMI.Methods In this retrospective study,we analyzed the records of 2 791 patients with acute STEMI admitted to Beijing Anzhen Hospital Affiliated to Capital Medical University between January 2018 and January 2022.展开更多
Objective To monitor hemodynamic changes during serial balloon pulmonary angioplasty (BPA) for chronic thromboembolic pulmonary hypertension (CTEPH).Methods General clinical data of CTEPH patients diagnosed from Octob...Objective To monitor hemodynamic changes during serial balloon pulmonary angioplasty (BPA) for chronic thromboembolic pulmonary hypertension (CTEPH).Methods General clinical data of CTEPH patients diagnosed from October 2017 to January 2022 in Beijing Chaoyang Hospital were collected,and 83 patients who underwent at least 1 BPA treatment were included to analyze their 6 min walking distance,WHO functional class,N-terminal B-type natriuretic peptide precursor(NT-proBNP),troponin I (cTnI) and haemodynamic indices.展开更多
Objective To explore the relationship between pathogenic gene,mutation and phenotype of left ventricular noncompaction (LVNC) patients and their family members.Methods The subjects were the proband with LVNC and her f...Objective To explore the relationship between pathogenic gene,mutation and phenotype of left ventricular noncompaction (LVNC) patients and their family members.Methods The subjects were the proband with LVNC and her family members.The medical history including electrocardiogram,echocardiography and cardiac magnetic resonance examination of the proband and family members was collected.展开更多
Medulloepithelioma,a rare tumor,arises from the epithelium of the medullary tube.In this article,we present a 3-year-old.boy who suffered from secondary glaucoma,initially presumed the primary disease was endophthalmi...Medulloepithelioma,a rare tumor,arises from the epithelium of the medullary tube.In this article,we present a 3-year-old.boy who suffered from secondary glaucoma,initially presumed the primary disease was endophthalmitis.Subconjunctival mass was later found,pathologically proved to be medulloepithelioma.We discuss the patient management with emphasis on the early signs of examination and the role of ultrabiomicroscopy （UBM） in evaluating pediatric secondary glaucoma and in influencing the management of patients with medulloepithelioma.展开更多
Background Multiple apical muscular ventricular septal defects （VSDs） remain a challenge for surgeons because of their anatomical features. We used single patch with intermediate fixations to repair multiple apical ...Background Multiple apical muscular ventricular septal defects （VSDs） remain a challenge for surgeons because of their anatomical features. We used single patch with intermediate fixations to repair multiple apical muscular VSDs through right ventricuiotomy. Methods We analysed the data of 16 children （median age 8 months, range 2 months to 144 months） with multiple apical muscular VSDs who underwent a single patch technique via apical right ventdcu^otomy. Perioperative data were collected and analysed, and the patients were followed up for three months to 66 months （median, 46 months） to investigate the outcomes. Results All patients recovered from cardiopulmonary bypass easily with median of cardiopulmonary bypass time 87 minutes and of aortic crossclamp time 53 minutes. No surgically related death occurred and no patient required reoperation. One patient died of pseudomonas pyocyanea infection on day 11 postoperatively. Residual shunt happened in one patient with a diameter of 2 mm and spontaneously closed in 12 months after operation. Two patients presented slightly reduced right ventricular volume and apical hypokinesia postoperatively and recovered 24 and 36 months later respectively. No other complication was found during the three months to 66 months follow-up. Conclusion Our experiences using a single patch technique with intermediate fixations via right ventriculotomy in the management of multiple muscular VSDs had favourable outcomes.展开更多
Objective To evaluate the clinical efficacy and safety of integrative medical program based on blood cooling and detoxification recipe(BCDR)in treating patients with hepatitis B virus related acute-on-chronic liver fa...Objective To evaluate the clinical efficacy and safety of integrative medical program based on blood cooling and detoxification recipe(BCDR)in treating patients with hepatitis B virus related acute-on-chronic liver failure(HBV-ACLF)of heat-toxicity accumulation syndrome(HTAS).Methods Adopting randomized展开更多
文摘BACKGROUND: The indocyanine green (ICG) clearance test (clearance rate (K) and retention rate at 15 minutes (R(15))) is a sensitive indicator to evaluate liver function. The model for end-stage liver disease (MELD) score has emerged as a useful tool for estimating the mortality of patients awaiting liver transplantation and has recently been validated on patients with liver diseases of various etiologies and severity. In this study, we investigated the correlation between the ICG clearance test and MELD score of patients with liver cirrhosis. METHODS: From June 2007 to March 2008, 52 patients with liver cirrhosis admitted to our center were classified into Child-Pugh class A (8 patients), B (14) and C (30). The ICG clearance test (K value and R(15)) was performed by ICG pulse spectrophotometry (DDG-3300K), and the MELD scores of patients were calculated. RESULTS: As the Child-Pugh classification of liver function gradually deteriorated, the K value decreased, while R(15) and MELD score increased. There were significant statistical differences in K value, R(15) and MELD score in patients with different Child-Pugh classifications. Significant correlations were found between the parameters of the ICG clearance test (K value and R(15)) and MELD score. A negative correlation was observed between K value and MELD score (r=-0.892, P < 0.05), while a positive correlation was observed between R(15) and MELD score (r=0.804, P < 0.05). CONCLUSIONS: The ICG clearance test and MELD score are good parameters for evaluating liver function. Moreover, K value and R(15) have significant correlations with MELD score, especially the K value, which may be a convenient and appropriate indicator to evaluate liver function of patients with liver cirrhosis.
基金Supported by The Science Technology Program of Beijing Education Committee, No. KM200810025002
文摘AIM:To study characteristics of collateral circulation of gastric varices (GVs) with 64-row multidetector computer tomography portal venography (MDCTPV).METHODS:64-row MDCTPV with a slice thickness of 0.625 mm and a scanning field from 2 cm above the tracheal bifurcation to the lower edge of the kidney was performed in 86 patients with GVS diagnosed by endoscopy. The computed tomography protocol included unenhanced,arterial and portal vein phases. The MDCTPV was performed on an AW4.3 workstation. GVs were classified into three types according to Sarin's Classification. The afferent and efferent veins of each type of GV were observed.RESULTS:The afferent venous drainage originated mostly from the left gastric vein alone (LGV) (28/86,32.59%),or the LGV more than the posterior gastric vein/short gastric vein [LGV > posterior gastric vein/short gastric vein (PGV/SGV)] (22/86,25.58%),as seen by MDCTPV. The most common efferent venousdrainage was via the azygos vein to the superior vena cava (53/86,61.63%),or via the gastric/splenorenal shunt (37/86,43.02%) or inferior phrenic vein (8/86,9.30%) to the inferior vena cava. In patients with gastroesophageal varices type 1,the afferent venous drainage of GV mainly originated from the LGV or LGV > PGV/SGV (43/48,89.58%),and the efferent venous drainage was mainly via the azygos vein to the super vena cava (43/48,89.58%),as well as via the gastric/splenorenal shunt (8/48,16.67%) or inferior phrenic vein (3/48,6.25%) to the inferior vena cava. In patients with gastroesophageal varices type 2,the afferent venous drainage of the GV mostly came from the PGV/SGV more than the LGV (PGV/SGV > LGV) (8/16,50%),and the efferent venous drainage was via the azygos vein (10/16,62.50%) and gastric/splenorenal shunt (9/16,56.25%). In patients with isolated gastric varices,the main afferent venous drainage was via the PGV/SGV alone (16/22,72.73%),and the efferent venous drainage was mainly via the gastric/splenorenal shunt (20/22,90.91%),as well as the inferior phrenic vein (3/23) to the inferior vena cava. CONCLUSION:MDCTPV can clearly display the afferent and efferent veins of all types of GV,and it could provide useful reference information for the clinical management of GV bleeding.
文摘BACKGROUND: Autoimmune pancreatitis (All?) is a unique chronic inflammation of the pancreas in which autoimmune mechanisms are involved in the pathogenesis. It is characterized by clinical, histopathological, radiographic, serologic and therapeutic features. Since it was first described in 1995, increasing numbers of AIP patients have been diagnosed. METHODS: In this study, the data from 2 patients with AIP who had elevation of serum immunoglobulin G, positive autoantibody, swelling of the pancreas and narrowing of the main pancreatic duct on imaging were analyzed retrospectively. RESULTS: With the initial diagnosis of AIP, both patients underwent regular prednisone therapy, which was initiated at a dose of 40 mg per day with a tapering schedule of 5 mg every 2 weeks. The patients responded very well to treatment with prednisone. CONCLUSIONS: AIP is a relatively new disease entity. Although it is diagnosed by distinct characteristics of the clinical, radiologic, histopathologic, and serologic findings, many questions require further clarification, including its relationship to other autoimmune diseases and misdiagnosis as pancreaticobiliary malignancies.
文摘BACKGROUND: Triptolide (TPT) is a diterpenoid triepoxide extracted from the Chinese herb Tripterygium wilfordii Hook. F. It exhibits potent immunosuppressive and anti-inflammatory properties. This study was undertaken to investigate its effects on prolongation of islet allograft survival in rodents. Additionally, we investigated whether TPT would be toxic to islet function in vivo. METHODS: We transplanted BALB/c islets to either chemically induced diabetic C57BL/6 mice or spontaneously diabetic non-obese diabetic (NOD) mice. TPT was injected within 2 weeks or continuously, until rejection, in the two combinations. Then, we evaluated the toxicity of TPT on islet function by daily injection to naive BALB/c or diabetic BALB/c that was cured by syngeneic islet transplantation under the kidney capsule. Mice injected with cyclosporine A (CsA) or vehicle served as controls. Intraperitoneal glucose tolerance tests (IPGTTs) performed at 4 and 8 weeks in the naive BALB/c group, and at 2, 4, 6, and 8 weeks in the syngeneic transplanted group. RESULTS: The medium survival time of islets allograft from TPT treated C57BL/6 and NOD recipients were 28.5 days (range 24-30 days, n=10) and 33.0 days (range 15-47 days, n=6), respectively, and they were significantly different from those of the vehicle treated controls, which were 14.0 days (range 13-16 days, n=6) and 5.0 days (range 4-10 days, n=6), respectively (all P<0.0001). The IPGTT demonstrated that there was no difference between the TPT treated and vehicle treated groups, either in the normal or syngeneic transplanted islet BALB/c mice. However, CsA injection impaired islet function in both normal and syngeneic transplanted mice as early as 4 weeks. CONCLUSION: TPT prolonged islets allograft survival in a chemically induced diabetic or an autoimmune diabetic murine model without impairment of islet function. (Hepatobiliary Pancreat Dis Int 2010; 9: 312-318)
基金the National Basic Research Program of China-973 Project,No. 2006CB500700the National Natural Science Foundation of China,No. 30500255the Natural Science Foundation of Beijing,No. 5102010
文摘Senegenin, an effective component of Polygala tenuifolia root extract, promotes proliferation and differentiation of neural progenitor cells in the hippocampus. However, the effects of senegenin on mesencephalon-derived neural progenitor cells remain poorly understood. Cells from a ventral mesencephalon neural progenitor cell line （ReNcell VM） were utilized as models for pharmaceutical screening. The effects of various senegenin concentrations on cell proliferation were analyzed, demonstrating that high senegenin concentrations （5, 10, 50, and 100 μmol/L）, particularly 50 μmol/L, significantly promoted proliferation of ReNcell VM cells. In the mitogen-activated protein kinase signal transduction pathway, senegenin significantly increased phosphorylation levels of extracellular signal-regulated kinases. Moreover, cell proliferation was suppressed by extracellular signal-regulated kinase inhibitors. Results suggested that senegenin contributed to in vitro proliferation of human neural progenitor cells by upregulating phosphorylation of extracellular signal-regulated kinase.
文摘BACKGROUND: Interleukin-24 (IL-24) is a novel candidate tumor suppressor that induces tumor cell apoptosis experimentally in a variety of human malignant cells including liver cancer cells. The present study was conducted to investigate the potential effect of recombinant adeno-associated virus (rAAV)-mediated IL-24 gene therapy on tumor recurrence and metastasis by inducing tumor cell apoptosis in a hepatocellular carcinoma (HCC) model in nude mice. METHODS: We established a recurrent and metastatic HCC model in nude mice and constructed an rAAV vector carrying alpha-fetoprotein (AFP) promoter for expressing the IL-24 gene (rAVV/AFP/IL-24). The vector was administered by regional injection (liver incisal margin). AFP was detected by radiation immunoassay. Histological evaluation of tumor recurrence and metastasis was performed for the liver and lung. The effect of tumor cell apoptosis was confirmed by TUNEL analysis. RESULTS: IL-24 gene therapy prevented tumor recurrence and metastasis, as evidenced by marked decreases in the number of metastatic tumor nodules and tumor volume in the liver and lung. At the same time, serum AFP concentration decreased markedly in the IL-24 group compared with the control or rAAV groups (P<0.05). IL-24 gene therapy inhibited tumor recurrence and metastasis as evidenced by the induction of tumor cell apoptosis. CONCLUSION: The results demonstrated that targeted IL-24 gene therapy was effective in the prevention of postoperative recurrence and metastasis in an HCC nude mice model by induction of tumor cells apoptosis with potential minimum tumor burden.
文摘BACKGROUND: The ischemic-type biliary lesion (ITBL) is one of the most serious biliary complications of liver transplantation. This study aimed to investigate the effects of autologous bone marrow mononuclear cell (BM-MNC) implantation on neovascularization and the prevention of intrahepatic ITBL in a rabbit model. METHODS: The rabbits were divided into control, experimental model, and cell implantation groups, with 10 in each group. The model of intrahepatic ITBL was established by clamping the hepatic artery and common bile duct. Autologous BM-MNCs were isolated from the tibial plateau by density gradient centrifugation and were implanted through the common hepatic artery. Changes in such biochemical markers as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma-glutamyltranspeptidase, total bilirubin and direct bilirubin were measured. Four weeks after operation, cholangiography, histopathological manifestations, differentiation of BM-MNCs, microvessel density and the expression of vascular endothelial growth factor were assessed. RESULTS: Compared with the experimental model group, the BM-MNC implantation group showed superiority in the time to recover normal biochemistry. The microvessel density and vascular endothelial growth factor expression of the implantation group were significantly higher than those of the control and experimental model groups. The ITBL in the experimental model group was more severe than that in the implantation group and fewer new capillary blood vessels occurred around it. CONCLUSIONS: Implanted autologous BM-MNCs can differentiate into vascular endothelial cells, promote neovascularization and improve the blood supply to the ischemic bile duct, and this provides a new way to diminish or prevent intrahepatic ITBL after liver transplantation. (Hepatobiliary Pancreat Dis Int 2010; 9:593-599)
文摘Superwarfarins are a class of rodenticides. Gastrointestinal hemorrhage is a fatal complication of superwarfarin poisoning, requiring immediate treatment. Here, we report a 55-year-old woman with tardive upper gastrointestinal hemorrhage caused by superwarfarin poisoning after endoscopic cold mucosal biopsy.
基金Supported by Program for Outstanding Medical Field Leaders,Beijing,No. 2009-1-03National Natural Science Foundation of China,No. 30772123+1 种基金New Century Educational Talents Plan of Ministry of Education,China,No. NCET-05-0200Youth Foundation of Beijing Chaoyang Hospital
文摘AIM:To investigate the eff icacy of acellular dermal matrix(ADM) for intestinal elongation in animal models.METHODS:Japanese white big-ear rabbits(n = 9) and Wuzhishan miniature pigs(n = 5) were used in the study.Home-made and commercial ADM materials were used as grafts,respectively.A 3-cm long graft was interposed in continuity with the small bowel and a sideto-side anastomosis,distal to the graft about 3-4 cm,was performed.The animals were sacrificed at 2 wk,4 wk,8 wk and 3 mo after surgery and the histological changes were evaluated under light microscope and electron microscope.RESULTS:The animals survived after the operation with no evidence of peritonitis and sepsis.Severe ad-hesions were found between the graft and surrounding intestine.The grafts were completely absorbed within postoper ative two or three months except one.Histological observ ation showed inflammation in the grafts with fibrinoid necroses,infiltration of a large amount of neutrophils and leukomonocytes,and the degree varied in different stages.The neointestine with wellformed structures was not observed in the study.CONCLUSION:It is not suitable to use acellular dermal matrix alone as a scaffold for the intestinal elongation in animal models.
文摘Objective To analyze the disease characteristics of aspiration pneumonia and its risk factors.Methods In thisretrospective case-control study,analysis was conducted on data from 92 patients aged≥60 years admitted to Beijing Jishuitan Hospital,Capital Medical University between June 1,2018 and July 31,2022,with aspiration pneumonia(AsP)as the primary diagnosis at the time of hospital discharge and from non-AsP patients admitted during the same period.The number of participants was matched at a 1:1 ratio.Results The average age of the AsP group was(80.88±9.41)years and 57(62.0%)were men.The average age of the control group was(77.74±10.98)years and 52(56.5%)were men.There was no statistically significant difference in age and sex ratio(age:t=1.973,P=0.060;sex ratio:X^(2)=0.661,P=0.416).Univariate analysis showed that,at admission,body mass index(BMI)and activities of daily living(ADL)scores of the AsP group were lower than those of the non-AsP group.
文摘Objective To examine the clinical subtypes of patients with multisystem atrophy(MSA)that may indicate the prognosis of patients.Additionally,we aim to compare the ability to perform daily activities among patients of each subtype using cluster analysis.Methods The retrospective analysis included demographic data,clinical symptoms and signs,scale scores,and ancillary examinations of 94 patients diagnosed with multisystem atrophyatXuanwu Hospital of Capital Medical University.The study aimed to analyze the clinical characteristicssofeachhsubtype obtained through clustering.Additionally,acomparison was'made between patients with traditional motor subtypes and those with new subtypes in terms of activities of daily living.The study consisted of 94 MSA patients,with an average age of 61 years and a female representation of 51.1%.Using the data collected on the continuum,a full linkage hierarchical cluster analysis was performed to classify MSA patients into four clinical subtypes:gait disorder(17 cases,18.1%),malignant tonic hyperkinetic with premature haircut(25 cases,26.6%),intermediate(43 cases,45.7%)。
文摘Objective To investigate the association between body mass index (BMI) and waist circumference (WC)with all-cause mortality in middle-aged and elderly patients receiving maintenance hemodialysis (MHD).Methods The clinical data of MHD patients aged≥50years old from eleven hemodialysis centers from April to June 2017 in Beijing were analyzed.
文摘Objective To analyze the diagnostic efficacy of urinary lipoarabinomannan (LAM) antigen detection method in tuberculosis patients,and to provide an experimental basis for the clinical application of urinary LAM kit in China.Methods From March to May 2023,228 patients with lung diseases [134 male,94 female,age 20-82 (44.8±16.7) years] were prospectively collected in Beijing Chest Hospital,Capital Medical University,including 143 pulmonary tuberculosis patients and 85 non-tuberculosis patients.
文摘Objective To investigate the in-hospital incidence,associated outcomes,and predictors of UGIB after STEMI.Methods In this retrospective study,we analyzed the records of 2 791 patients with acute STEMI admitted to Beijing Anzhen Hospital Affiliated to Capital Medical University between January 2018 and January 2022.
文摘Objective To monitor hemodynamic changes during serial balloon pulmonary angioplasty (BPA) for chronic thromboembolic pulmonary hypertension (CTEPH).Methods General clinical data of CTEPH patients diagnosed from October 2017 to January 2022 in Beijing Chaoyang Hospital were collected,and 83 patients who underwent at least 1 BPA treatment were included to analyze their 6 min walking distance,WHO functional class,N-terminal B-type natriuretic peptide precursor(NT-proBNP),troponin I (cTnI) and haemodynamic indices.
文摘Objective To explore the relationship between pathogenic gene,mutation and phenotype of left ventricular noncompaction (LVNC) patients and their family members.Methods The subjects were the proband with LVNC and her family members.The medical history including electrocardiogram,echocardiography and cardiac magnetic resonance examination of the proband and family members was collected.
文摘Medulloepithelioma,a rare tumor,arises from the epithelium of the medullary tube.In this article,we present a 3-year-old.boy who suffered from secondary glaucoma,initially presumed the primary disease was endophthalmitis.Subconjunctival mass was later found,pathologically proved to be medulloepithelioma.We discuss the patient management with emphasis on the early signs of examination and the role of ultrabiomicroscopy （UBM） in evaluating pediatric secondary glaucoma and in influencing the management of patients with medulloepithelioma.
文摘Background Multiple apical muscular ventricular septal defects （VSDs） remain a challenge for surgeons because of their anatomical features. We used single patch with intermediate fixations to repair multiple apical muscular VSDs through right ventricuiotomy. Methods We analysed the data of 16 children （median age 8 months, range 2 months to 144 months） with multiple apical muscular VSDs who underwent a single patch technique via apical right ventdcu^otomy. Perioperative data were collected and analysed, and the patients were followed up for three months to 66 months （median, 46 months） to investigate the outcomes. Results All patients recovered from cardiopulmonary bypass easily with median of cardiopulmonary bypass time 87 minutes and of aortic crossclamp time 53 minutes. No surgically related death occurred and no patient required reoperation. One patient died of pseudomonas pyocyanea infection on day 11 postoperatively. Residual shunt happened in one patient with a diameter of 2 mm and spontaneously closed in 12 months after operation. Two patients presented slightly reduced right ventricular volume and apical hypokinesia postoperatively and recovered 24 and 36 months later respectively. No other complication was found during the three months to 66 months follow-up. Conclusion Our experiences using a single patch technique with intermediate fixations via right ventriculotomy in the management of multiple muscular VSDs had favourable outcomes.
文摘Objective To evaluate the clinical efficacy and safety of integrative medical program based on blood cooling and detoxification recipe(BCDR)in treating patients with hepatitis B virus related acute-on-chronic liver failure(HBV-ACLF)of heat-toxicity accumulation syndrome(HTAS).Methods Adopting randomized
