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Repair of articular cartilage defects in minipigs by microfracture surgery and BMSCs transplantation 被引量:1
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作者 左镇华 杨柳 +1 位作者 段小军 郭林 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第5期288-292,共5页
Objective:To investigate the feasibility of minimal invasive repair of cartilage defect by arthroscope-aided microfracture surgery and autologous transplantation of mesenchymal stem cells. Methods: Bone marrow of mini... Objective:To investigate the feasibility of minimal invasive repair of cartilage defect by arthroscope-aided microfracture surgery and autologous transplantation of mesenchymal stem cells. Methods: Bone marrow of minipigs was taken out and the bone marrow derived mesenchymal stem cells (BMSCs) were isolated and cultured to passage 3. Then 6 minipigs were randomly divided into 2 groups with 6 knees in each group. After the articular cartilage defect was induced in each knee, the left defect received microfracture surgery and was injected with 2.5 ml BMSCs cells at a concentration of 3×107 cells/ml into the articular cavity; while right knee got single microfracture or served as blank control group. The animals were killed at 8 or 16 weeks, and the repair tissue was histologically and immunohistochemically examined for the presence of type Ⅱ collagen and glycosaminoglycans (GAGs) at 8 and 16 weeks. Results: Eight weeks after the surgery, the overlying articular surface of the cartilage defect showed normal color and integrated to adjacent cartilage. And 16 weeks after surgery, hyaline cartilage was observed at the repairing tissues and immunostaining indicated the diffuse presence of this type Ⅱ collagen and GAGs throughout the repair cartilage in the treated defects. Single microfracture group had the repairing of fibrocartilage, while during the treatment, the defects of blank group were covered with fewer fiber tissues, and no blood capillary growth or any immunological rejection was observed. Conclusion: Microfracture technique and BMSCs transplantation to repair cartilage defect is characterized with minimal invasion and easy operation, and it will greatly promote the regeneration repair of articular cartilage defect. 展开更多
关键词 cartilage defect MICROFRACTURE bone marrow derived mesenchymal stem cells cartilage regeneration
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Fabrication and Evaluation of Porous Keratin/chitosan(KCS) Scaffolds for Effectively Accelerating Wound Healing 被引量:2
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作者 TAN Hong Bo WANG Fu You +7 位作者 DING Wei ZHANG Ying DING Jing CAI Di Xin YU Kai Fu YANG Jun YANG Liu XU Yong Qing 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第3期178-189,共12页
Objective To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. Methods The chitosan with different concentrations... Objective To develop a dressing with desired antibacterial activity, good water maintaining ability and mechanical properties for wound healing and skin regeneration. Methods The chitosan with different concentrations were added in keratin solution to form porous keratin/chitosan(KCS) scaffolds. The morphological characteristics, chemical composition, wettability, porosity, swelling ratio and degradation of the scaffolds were evaluated. The antibacterial activity was tested by using S. aureus and E. coli suspension for 2 h. And L929 fibroblast cells culture was used to evaluate the cytotoxicity of the KCS scaffolds. Results The adding of chitosan could increase the hydrophobicity, decrease porosity, swelling ratio and degradation rate of the KCS porous scaffolds. Mechanical properties of KCS scaffolds could be enhanced and well adjusted by chitosan. KCS scaffolds could obviously decrease bacteria number. The proliferation of fibroblast cells in porous KCS patch increased firstly and then decreased with the increase of chitosan concentration. It was appropriate to add 400 μg/m L chitosan to form porous KCS scaffold for achieving best cell attachment and proliferation compared with other samples. Conclusion The porous KCS scaffold may be used as implanted scaffold materials for promoting wound healing and skin regeneration. 展开更多
关键词 Keratin Chitosan Porous scaffold Wound healing Skin regeneration
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Arthroscopically assisted treatment for Schatzker type Ⅰ-Ⅴ tibial plateau fractures 被引量:25
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作者 段小军 杨柳 +2 位作者 郭林 陈光兴 戴刚 《Chinese Journal of Traumatology》 CAS 2008年第5期288-292,共5页
Objective: To report the clinical outcome of arthroscopically assisted treatment for tibial plateau fractures. Methods: A total of 39 patients with tibial plateau fractures were treated by arthroscopic fixation fro... Objective: To report the clinical outcome of arthroscopically assisted treatment for tibial plateau fractures. Methods: A total of 39 patients with tibial plateau fractures were treated by arthroscopic fixation from February 2002 to December 2005, including 11 patients with bony avulsion of the anterior cruciate ligament and 19 with meniscal injury. There were 4 cases of type Ⅰ fracture, 12 type Ⅱ, 9 type Ⅲ, 12 type Ⅳ and 2 type Ⅴ according to Schatzker criteria. Firstly, the combined injuries were treated. Then the plateau fractures with the displacement over 3 mm or more were reduced and fixed. Finally, the internal fixation was observed by X-ray equipment. Postoperative management was early motion and delayed weight bearing. Results: All the fractures healed in 3 or 4 months. All patients were followed up for 1 to 5 years after operation.No case had severe complications, such as poor wound healing, infection, osteofascial compartment syndrome and osteoarthfitis. According to the Rasmussen scoring system, 36 cases obtained excellent or good results and the other 3 cases had moderate clinical results. The average score was 26 ± 3. Conclusions: As an adjuvant treatment of intraarticular fractures such as tibial plateau fracture, arthroscopy has many advantages. It can treat associated intraarticular soft tissue components, visualize the chondral surface reduction, lavage the hematoma and smaller loose fragments, decrease soft tissue dissection, reduce the risk of scarring and promote rapid recovery. 展开更多
关键词 Tibial plateau fracture ARTHROSCOPY TREATMENT Surgical technique
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Hemi-arthroplasty performed in a 109-year-old patient with intertrochanteric fracture: A case report 被引量:5
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作者 Peng-Fei Yang Hao Chen +4 位作者 xiao-Jun Duan Guang-Xing Chen Lin Guo Hua-Quan Fan Liu Yang 《Chinese Journal of Traumatology》 CAS CSCD 2017年第6期352-354,共3页
The mortality of conservative treatment and the risk resulting from operation for elderly patients with femoral intertrochanteric fractures are high. Safety in the perioperative period and quicker recovery should be p... The mortality of conservative treatment and the risk resulting from operation for elderly patients with femoral intertrochanteric fractures are high. Safety in the perioperative period and quicker recovery should be placed at the top priority for elderly patients with hip fractures. We reported a case of 109- year-old female patient with intertrochanteric fracture who has undergone the hemiarthroplasty in our center recently. With sciatic nerve and lateral cutaneous nerve block anesthesia, she was offered the artificial femoral head replacement in the lumbar plexus block after sufficient preoperative preparation. The surgery went well with minimally invasive cut, and the patient's recovery was satisfactory. 展开更多
关键词 Aged Hip fractures HEMIARTHROPLASTY
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Properties of deproteinized bone for reparation of big segmental defect in long bone 被引量:5
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作者 简月奎 田晓滨 +4 位作者 李波 邱冰 周焯家 杨震 李起鸿 《Chinese Journal of Traumatology》 CAS 2008年第3期152-156,共5页
Objective: To explore suitable scaffold material for big segmental long bone defect by studying the properties of the prepared deproteinized bone. Methods: Cancellated bone were made as 30 mm × 3 mm × 3 m... Objective: To explore suitable scaffold material for big segmental long bone defect by studying the properties of the prepared deproteinized bone. Methods: Cancellated bone were made as 30 mm × 3 mm × 3 mm bone blocks from inferior extremity of pig femur along bone trabecula. The deproteinized bone was prepared with an improved method. Their morphological features, components, cell compatibility, mechanical and immunological properties were investigated respectively. Results: Deproteinized bone maintained natural re-ticular pore system. The main organic material is collagen I and inorganic composition is hydroxyapatite. It has good mechanical properties, cell adhesion rate and histocompatibility. Conlusion: This deproteinized bone can be applicable as scaffold for reparation of big segmental defect in long bone. 展开更多
关键词 Deproteinized bone Long bone Bone defect
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Preparation and characterization of methacrylated gelatin/bacterial cellulose composite hydrogels for cartilage tissue engineering 被引量:4
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作者 Liling Gu Tao Li +7 位作者 Xiongbo Song Xianteng Yang Senlei Li Long Chen Pingju Liu Xiaoyuan Gong Cheng Chen Li Sun 《Regenerative Biomaterials》 SCIE EI 2020年第2期195-202,共8页
Methacrylated gelatin(GelMA)/bacterial cellulose(BC)composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking.The morphology of GelMA/BC hydrogel wa... Methacrylated gelatin(GelMA)/bacterial cellulose(BC)composite hydrogels have been successfully prepared by immersing BC particles in GelMA solution followed by photo-crosslinking.The morphology of GelMA/BC hydrogel was examined by scanning electron microscopy and compared with pure GelMA.The hydrogels had very well interconnected porous network structure,and the pore size decreased from 200 to 10 mm with the increase of BC content.The composite hydrogels were also characterized by swelling experiment,X-ray diffraction,thermogravimetric analysis,rheology experiment and compressive test.The composite hydrogels showed significantly improved mechanical properties compared with pure GelMA.In addition,the biocompatility of composite hydrogels were preliminarily evaluated using human articular chondrocytes.The cells encapsulated within the composite hydrogels for 7 days proliferated and maintained the chondrocytic phenotype.Thus,the GelMA/BC composite hydrogels might be useful for cartilage tissue engineering. 展开更多
关键词 methacrylated gelatin bacterial cellulose HYDROGEL cartilage tissue engineering
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Ca_v3.3-mediated endochondral ossification in a three-dimensional bioprinted Gel MA hydrogel
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作者 Zhi Wang Xin Wang +6 位作者 Yang Huang Junjun Yang Zu Wan Zhenlan Fu Xiaoyuan Gong Guangxing Chen Liu Yang 《Bio-Design and Manufacturing》 SCIE EI CAS 2024年第6期983-999,共17页
The growth plate(GP)is a crucial tissue involved in skeleton development via endochondral ossification(EO).The bone organoid is a potential research model capable of simulating the physiological function,spatial struc... The growth plate(GP)is a crucial tissue involved in skeleton development via endochondral ossification(EO).The bone organoid is a potential research model capable of simulating the physiological function,spatial structure,and intercellular communication of native GPs.However,mimicking the EO process remains a key challenge for bone organoid research.To simulate this orderly mineralization process,we designed an in vitro sh Ca_(v)3.3 ATDC5-loaded gelatin methacryloyl(Gel MA)hydrogel model and evaluated its bioprintability for future organoid construction.In this paper,we report the first demonstration that the T-type voltage-dependent calcium channel(T-VDCC)subtype Ca_(v)3.3 is dominantly expressed in chondrocytes and is negatively correlated with the hypertrophic differentiation of chondrocytes during the EO process.Furthermore,Ca_(v)3.3 knockdown chondrocytes loaded with the Gel MA hydrogel successfully captured the EO process and provide a bioink capable of constructing layered and orderly mineralized GP organoids in the future.The results of this study could therefore provide a potential target for regulating the EO process and a novel strategy for simulating it in bone organoids. 展开更多
关键词 Bone organoid Endochondral ossification T-type voltage-dependent calcium channel(T-VDCC) Ca_(v)3.3 3D bioprinting
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Study on the correlation between early three-dimensional gait analysis and clinical efficacy after robot-assisted total knee arthroplasty 被引量:1
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作者 Rui He Ran Xiong +4 位作者 Mao-Lin Sun Jun-Jun Yang Hao Chen Peng-Fei Yang Liu Yang 《Chinese Journal of Traumatology》 CAS CSCD 2023年第2期83-93,共11页
Purpose:Robot-assisted technology is a forefront of surgical innovation that improves the accuracy of total knee arthroplasty (TKA).But whether the accuracy of surgery can improve the clinical efficacy still needs fur... Purpose:Robot-assisted technology is a forefront of surgical innovation that improves the accuracy of total knee arthroplasty (TKA).But whether the accuracy of surgery can improve the clinical efficacy still needs further research.The purpose of this study is to perform three-dimensional (3D) analysis in the early postoperative period of patients who received robot-assisted total knee arthroplasty (RATKA),and to study the trend of changes in gait parameters after RATKA and the correlation with the early clinical efficacy.Methods:Patients who received RATKA in the Center of Joint Surgery,the First Hospital Affiliated to Army Military Medical University from October 2020 to January 2021 were included.The imaging parameters,i.e.,hip-knee-ankle angle,lateral distal femoral angle,medial proximal tibial angle,posterior condylar angle were measured 3 months post-TKA.The 3D gait analysis and clinical efficacy by Western Ontario Mac Master University Index (WOMAC) score were performed pre-TKA,3 and 6 months post-TKA.The differences in spatiotemporal parameters of gait,kinetic parameters,and kinematic parameters of the operated limb and the contralateral limb were compared.The correlation between gait parameters and WOMAC scores was analyzed.Paired samplet-test and Wilcoxon rank-sum test were used to analyze the difference between groups,and Spearman correlation coefficient was used to analyze the correlation.Results:There were 31 patients included in this study,and the imaging indexes showed that all of them returned to normal post-TKA.The WOMAC score at 3 months post-TKA was significantly lower than that pre-TKA,and there was no significant difference between at 3 and 6 months.The 3D gait analysis results showed that the double support time of the operated limb reduced at 3 and 6 months (allp < 0.05),the maximum extension and maximum external rotation of the knee joint increased at stance phase,and the maximum flexion angle,the range of motion and the maximum external rotation increased at swing phase.Compared with the preoperative data,there were significant improvements (allp < 0.05).Compared with the contralateral knee joint,the maximum external rotation of the knee joint at swing phase was smaller than that of the contralateral side,and the maximum flexion and extension moment was greater than that of the contralateral knee.The maximum external rotation moment of the joint was greater than that of the contralateral knee joint (p < 0.05).There was a negative correlation between the single support time pre-TKA and the WOMAC score at 3 months (p = 0.017),and the single support time at 3 months was negatively correlated with the WOMAC score at 6 months (p = 0.043).The cadence at 6 months was negatively correlated with the WOMAC score at 6 months (p = 0.031).The maximum knee extension at stance phase at 6 months was negatively correlated with the WOMAC score at 6 month (p = 0.048).The maximum external rotation at stance phase at 6 months was negatively correlated with the WOMAC score at 6 months (p = 0.024).Conclusion:The 3D gait analysis of RATKA patients is more sensitive than WOMAC score in evaluating the clinical efficacy.Trend of changes in gait parameters shows that the knee joint support,flexion and extension function,range of motion,external rotation and varus deformity moment of the patient were significantly improved at 3 months after surgery,and continued to 6 months after surgery.Compared with the contralateral knee,the gait parameters of the operated limb still has significant gaps in functionality,such as the external rotation and flexion and extension.The single support time,cadence,knee extension,and knee external rotation of the operated limb have a greater correlation with the postoperative WOMAC score.Postoperative rehabilitation exercises should be emphasized,which is of great value for improving the early efficacy of RATKA. 展开更多
关键词 Total knee arthroplasty Robot Gait analysis Knee joint OSTEOARTHRITIS Correlation study
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Arthroscopic treatment of ankle impingement syndrome
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作者 Xin Chen He-Qin Huang Xiao-Jun Duan 《Chinese Journal of Traumatology》 CAS CSCD 2023年第6期311-316,共6页
Arthroscopic treatment of ankle impingement syndrome(AIS)is a minimally invasive surgical procedure used to address symptoms caused by impingement in the ankle joint.This syndrome occurs when there is abnormal contact... Arthroscopic treatment of ankle impingement syndrome(AIS)is a minimally invasive surgical procedure used to address symptoms caused by impingement in the ankle joint.This syndrome occurs when there is abnormal contact between certain bones or soft tissues in the ankle,leading to pain,swelling,or limited range of motion.Traditionally,open surgery was the standard approach for treating AIS.However,with advancements in technology and surgical techniques,arthroscopic treatment has become a preferred method for many patients and surgeons.With improved visualization and precise treatment of the arthroscopy,patients can experience reduced pain and improved functionality,allowing them to return to their daily activities sooner.In this paper,we reviewed the application and clinical efficacy the of arthroscopic approach for treating AIS,hoping to provide a reference for its future promotion. 展开更多
关键词 Ankle impingement syndrome ARTHROSCOPY JOINT SURGERY Clinical treatment
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Study of biological safety of scaffold material with heterologous deproteinized bone 被引量:12
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作者 刘雷 唐康来 +2 位作者 杨柳 简月奎 李起鸿 《Chinese Journal of Traumatology》 CAS 2006年第4期234-237,共4页
To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods : Deproteinized bone ( 10 mm) and leaching liquor were made... To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods : Deproteinized bone ( 10 mm) and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test. Results : No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramusclar implantation and cytotoxity were observed. Conclusions: The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering. 展开更多
关键词 Transplantation heterogeneous Tissue engineering Biological safety
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A follow-up study of arthroscopic combined reconstruction of anterior and posterior cruciate ligaments with allograft patellar tendon 被引量:5
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作者 谢峰 杨柳 +2 位作者 郭林 代灿 韩雪松 《Chinese Journal of Traumatology》 CAS 2007年第6期334-338,共5页
Objective:To evaluate the therapeutic effect of combined reconstruction of anterior cruciate ligament (ACL)and posterior cruciate ligament(PCL) simultaneously by using allograft patellar tendon under arthroscopy. Meth... Objective:To evaluate the therapeutic effect of combined reconstruction of anterior cruciate ligament (ACL)and posterior cruciate ligament(PCL) simultaneously by using allograft patellar tendon under arthroscopy. Methods:From May 2003 to November 2005,10 cases of ruptured ACL and PCL were fixated with compressed screws and reconstructed under arthroscopy with allograft patellar tendon simultaneously.The clinical results were evaluated according to IKDC,Lysholm,and Tegner clinical rating scales. Results:All patients were followed up for 12-30 months(mean:18 months).At the last follow-up,there was no knee extension limitation and knee flexion was between 120°and 135°,with an average of 128.38°.The Lysholm score of the 10 cases was 66.5±5.6 before operation and 89.8±3.4 at last follow up.The difference was statistically significant(P<0.01).The average Tegner activity score decreased from 6.9±1.7(range.4-9)before injury to 5.5±1.6(rang:2-9)at the follow-up (P=0.53).At the end of follow-up,IKDC score was graded as A in 4 cases(40.0%),B in 5(50.0%),and C in 1(10.0%).Of the 10 patients,8 returned to the same sports level as before injury and 2 were under the level. Conclusion:Arthroscopic combined reconstruction of ACL and PCL with allograft patellar tendon has the advantages of minimal trauma in surgery and reliable satisfactory outcome. 展开更多
关键词 ARTHROSCOPY Anterior cruciate ligament Posterior cruciate ligament TRANSPLANTATION
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Exosomes:key players in cancer and potential therapeutic strategy 被引量:41
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作者 Jie Dai Yangzhou Su +7 位作者 Suye Zhong Li Cong Bang Liu Junjun Yang Yongguang Tao Zuping He Chao Chen Yiqun Jiang 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2020年第1期1199-1208,共10页
Exosomes are extracellular vesicles secreted by most eukaryotic cells and participate in intercellular communication.The components of exosomes,including proteins,DNA,mRNA,microRNA,long noncoding RNA,circular RNA,etc.... Exosomes are extracellular vesicles secreted by most eukaryotic cells and participate in intercellular communication.The components of exosomes,including proteins,DNA,mRNA,microRNA,long noncoding RNA,circular RNA,etc.,which play a crucial role in regulating tumor growth,metastasis,and angiogenesis in the process of cancer development,and can be used as a prognostic marker and/or grading basis for tumor patients.Hereby,we mainly summarized as followed:the role of exosome contents in cancer,focusing on proteins and noncoding RNA;the interaction between exosomes and tumor microenvironment;the mechanisms that epithelial-mesenchymal transition,invasion and migration of tumor affected by exosomes;and tumor suppression strategies based on exosomes.Finally,the application potential of exosomes in clinical tumor diagnosis and therapy is prospected,which providing theoretical supports for using exosomes to serve precise tumor treatment in the clinic. 展开更多
关键词 EXOSOMES INVASION DIAGNOSIS
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Biomechanical researches on tissue engineering bone constructed by deproteinated bone 被引量:3
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作者 简月奎 田晓滨 +5 位作者 李起鸿 李波 彭智 赵伟峰 王远政 杨震 《Chinese Journal of Traumatology》 CAS 2010年第1期32-36,共5页
Objective: To study biomechanical changes of newly formed bones 24 weeks after repairing large defects of long bones of goats using heterogeneous deproteinated bone (DPB) prepared by modified methods as an engineer... Objective: To study biomechanical changes of newly formed bones 24 weeks after repairing large defects of long bones of goats using heterogeneous deproteinated bone (DPB) prepared by modified methods as an engineering scaffold. Methods: According to a fully randomized design, 18 goats were evenly divided into three groups: normal bone control group (Group A), autologous bone group (Group B) and experimental group (Group C). Each goat in Groups B and C were subjected to the periosteum and bone defect at middle-lower part of the right tibia (20% of the whole tibia in length), followed by autologous bone or DPB plus autolognus MSCs + rhBMP2 implantation, respectively and semi- ring slot fixation; while goats in Group A did not perform osteotomy. At 24 weeks after surgery, biomechanical tests were carried out on the tibias. Results: At 24 weeks after surgery, the results of anticompression test on tibias in three groups were recorded by a functional recorder presented as linear pressure-deformation curve. The shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values were 10.74 MPa±1.23 MPa, 10. 11 MPa±1.35 MPa and 10.22 MPa±1.32 MPa and fracture compression rates were 26.82%±0.87%, 27.17%±0.75% and 28.22%±1.12% in Groups A, B and C, respectively. Comparisons of anti-compression ultimate pressures and fracture compression rates among three groups demonstrated no significant difference (PAB=0.415, PBC=0.494). Three-point antibend test on tibias was recorded as load-deformation curves, and the shapes of the curves and their change tendency were similar among three groups. The ultimate pressure values of the anti-bend test were 481.52 N±12.45 N, 478.34 N±14.68 N and 475.62 N±13.41 N and the fracture bend rates were 2.62 mm±0.12 mm, 2.61 mm±0.15 mm and 2.81 mm±0.13 mm in Groups A, B and C, respectively. There was no significant difference between groups (PAB=0.7, PBc=0.448). The ultimate anti-torsion torque values were 6.55 N.mi-0.25 N.m, 6.34 N'm^0.18 N'm and 6.42 N'm^0.21 N'm and fracture torsion rates were 29.51°±1.64°, 28.88±1.46° and 28.81°±1.33° in Groups A, B and C, respectively. There was no significant difference between groups (PAB=0.123, PBc=0.346). Conclusions: The biomechanical characteristics of newly formed bones from heterogeneous DPB for repairing large segmental long bone defect are comparable to those of normal bones and autologous bones. DPB has the potential for clinical usage as bone graft material. 展开更多
关键词 BIOMECHANICS Tissue engineering Deproteinated bone
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Immunological study on the transplantation of an improved deproteinized heterogeneous bone scaffold material in tissue engineering 被引量:2
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作者 刘雷 裴福兴 +2 位作者 屠重棋 周宗科 李起鸿 《Chinese Journal of Traumatology》 CAS 2008年第3期141-147,共7页
Objective: To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods: The fresh pig bone and deprote... Objective: To observe the immune response after the transplantation of a deproteinized heterogeneous bone scaffold and provides the theoretic reference for clinical practice. Methods: The fresh pig bone and deproteinized bone were transplanted respectively to establish BABL/C thigh muscle pouches model of male mice and take the samples for detection at 1, 2, 4, 6 weeks after operation. Lymphocyte stimulation index, subset analysis, serum specific antibody IgG, cytokine detection and topographic histologic reaction after implantation were investigated. Results: After the transplantation of deproteinized bone, lymphocyte stimulation index, CD4^+ and CD8^+ T-lymphocyte subsets, serum specific antibody IgG and cytokines in deproteinized bone group were significantly lower than those in fresh pig bone group at each time point (P〈0.05). The histological examination found that in fresh bone group at each time point, a large quantity of inflammatory cells infiltrated in the surrounding of bone graft, and they were mainly lymphocytes, including macrophages and monocytes. In deproteinized bone group, there were few inflammatory cells infiltration around bone graft one week after operation. The lymphocytes were decreased as time went by. At 6 weeks, fibroblasts and fibrous tissue grew into the graft, and osteoclasts and osteoprogenitor cells appeared on the verge. Conclusions: The established heterogeneous deproteinized bone has low immunogenicity and is a potentially ideal scaffold material for bone tissue engineering. 展开更多
关键词 Heterogeneous bone DEPROTEINIZATION Tissue engineering TRANSPLANTATION Immune reaction
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Preparation and physicochemical properties of scaffold materials of heterogeneous deproteinized bone 被引量:2
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作者 刘雷 李起鸿 +2 位作者 唐康来 杨柳 简月奎 《Chinese Journal of Traumatology》 CAS 2007年第1期59-62,共4页
Objective : To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone. Methods: Deproteinized bone was made through a series of physicochemica... Objective : To prepare and observe the physicochemical properties of scaffold materials of heterogeneous deproteinized tissue-engineered bone. Methods: Deproteinized bone was made through a series of physicochemical treatments in pig ribs and analyzed with histological observation, scanning electron microscopy, infrared spectrum, X-ray diffraction and energy dispersive analysis, Kjeidahl determination and mechanics analysis. Results: Interstitial collagen fiber was positive and mucin was negative in deproteinized bone, but, both were positive in fresh bone. Deproteinized bone maintained natural pore network. Its pore size was 472.51μm ± 7.02μm and the porosity was 78.15 % ± 6.45 %. The results of infrared spectrum showed that collagen was present in deproteinized bone. Both fresh and deproteinized bone had curve of hydroxyapatite. The Ca/P ratios were 1.71 ± 0. 95 and 1.68 ± 0. 76 ( P 〉 0. 05 ), and the protein contents were26.6% ± 2.23% and 19.1% ± 2.14% (P 〈 0.05) in fresh and deproteinized bone, respectively. There was no significant difference of destruction load under compression and maximal destruction load between fresh and deproteinized bone (P 〉 0. 05). The elastic modulus was higher in deproteinized bone than that in fresh bone (P 〈 0.05). Conclusions : Physicochemical properties and mechanic strength of deproteiulzed tissue-engineered bone meet the demands of ideal scaffold materials. But, its immunogenicity should be observed through further experiments for its clinical applications. 展开更多
关键词 Tissue engineering HYDROXYAPATITES Heterogeneous bone
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Tropoelastin improves adhesion and migration of intra-articular injected infrapatellar fat pad MSCs and reduces osteoarthritis progression 被引量:6
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作者 Junjun Yang Xin Wang +13 位作者 Yahan Fan Xiongbo Song Jiangyi Wu Zhenlan Fu Tao Li Yang Huang ZheXiong Tang Shuo Meng Na Liu Jiajia Chen Pingju Liu Liu Yang Xiaoyuan Gong Cheng Chen 《Bioactive Materials》 SCIE 2022年第4期443-459,共17页
Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in... Intra-articular injection of mesenchymal stem cells(MSCs)is a promising strategy for osteoarthritis(OA)treatment.However,more and more studies reveal that the injected MSCs have poor adhesion,migration,and survival in the joint cavity.A recent study shows that tropoelastin(TE)regulates adhesion,proliferation and phenotypic maintenance of MSCs as a soluble additive,indicating that TE could promote MSCs-homing in regenerative medicine.In this study,we used TE as injection medium,and compared it with classic media in MSCs intra-articular injection such as normal saline(NS),hyaluronic acid(HA),and platelet-rich plasma(PRP).We found that TE could effectively improve adhesion,migration,chondrogenic differentiation of infrapatellar fat pad MSCs(IPFP-MSCs)and enhance matrix synthesis of osteoarthritic chondrocytes(OACs)in indirect-coculture system.Moreover,TE could significantly enhance IPFP-MSCs adhesion via activation of integrin β1,ERK1/2 and vinculin(VCL)in vitro.In addition,intra-articular injection of TE-IPFP MSCs suspension resulted in a short-term increase in survival rate of IPFP-MSCs and better histology scores of rat joint tissues.Inhibition of integrin β1 or ERK1/2 attenuated the protective effect of TE-IPFP MSCs suspension in vivo.In conclusion,TE promotes performance of IPFP-MSCs and protects knee cartilage from damage in OA through enhancement of cell adhesion and activation of integrin β1/ERK/VCL pathway.Our findings may provide new insights in MSCs intra-articular injection for OA treatment. 展开更多
关键词 TROPOELASTIN OSTEOARTHRITIS Infrapatellar fat pad MSCs
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Cellular compatibility of improved scaffold material with deproteinized heterogeneous bone 被引量:1
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作者 刘雷 裴福兴 +1 位作者 周宗科 李起鸿 《Chinese Journal of Traumatology》 CAS 2007年第4期233-236,共4页
Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone m... Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone marrow stromal cells(BMSC)were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope,scanning electron microscope,MTT assay,flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth,adhesion in the material, cell cycle and cell viability. Results:The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation,differentiation and secretion function of BMSCs. Conclusions:The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering. 展开更多
关键词 Bone marrow cells HISTOCOMPATIBILITY SCAFFOLD
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Acute arterial occlusion in the midpiece of femoral artery following total knee arthroplasty: Report of one case 被引量:2
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作者 Rui He Liu Yang 《Chinese Journal of Traumatology》 CAS CSCD 2016年第2期116-118,共3页
Acute arterial occlusion is a rare complication following total knee arthroplasty (TKA). The incidence as reported previously is from 0.03% to 0.17%; however, the sequelae can be disastrous because of its potential ... Acute arterial occlusion is a rare complication following total knee arthroplasty (TKA). The incidence as reported previously is from 0.03% to 0.17%; however, the sequelae can be disastrous because of its potential threat to limb loss. We report a case of acute arterial occlusion in the midpiece of femoral artery following TKA occurred 40 rain postoperatively. The occlusion site existed at the midpiece of femoral artery is uncommon. Arterial circulation of the lower limb could not be restored by the thrombolysis and thrombectomy treatments performed within 11 h after TKA. In the end, amputation had to be carried out. In the treatment of acute arterial occlusion following TKA with a tourniquet, it is important to fully consider that arteriosclerosis may induce atheromatous plaque disruption, which might be the reason for acute arterial occlusion. 展开更多
关键词 Knee arthroplastyArterial occlusionThrombosis
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Laser Powder Bed Fusion-built Ti6Al4V Bone Scaffolds Composed of Sheet and Strut-based Porous Structures: Morphology, Mechanical Properties, and Biocompatibility 被引量:1
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作者 Shuai Ma Qian Tang +6 位作者 Changbao Zhu Fuyou Wang Qixiang Feng Jun Song Rossitza Setchi Chenglong Ma Ran Tao 《Chinese Journal of Mechanical Engineering(Additive Manufacturing Frontiers)》 2022年第4期87-99,共13页
Laser powder bed fusion(L-PBF)-built triply periodic minimal surface(TPMS)structures are designed by implicit functions and are endowed with superior characteristics,such as adjustable mechanical properties and light-... Laser powder bed fusion(L-PBF)-built triply periodic minimal surface(TPMS)structures are designed by implicit functions and are endowed with superior characteristics,such as adjustable mechanical properties and light-weight features for bone repairing;thus,they are considered as potential candidates for bone scaffolds.Unfortunately,previous studies have mainly focused on different TPMS structures.The fundamental understanding of the differences between strut and sheet-based structures remains exclusive,where both were designed by one formula.This consequently hinders their practical applications.Herein,we compared the morphology,mechanical properties,and biocompatibility of sheet and strut-based structures.In particular,the different properties and in vivo bone repair effects of the two structures are uncovered.First,the morphology characteristics demonstrate that the manufacturing errors of sheet-based structures with diverse porosities are comparable,and semi-melting powders as well as the ball phenomenon are observed;in comparison,strut-based samples exhibit cracks and thickness shrinking.Second,the mechanical properties indicate that the sheet-based structures have a greater elastic modulus,energy absorption,and better repeatability compared to strut-based structures.Furthermore,layer-by-layer fracturing and diagonal shear failure modes are observed in strut-based and sheet-based structures,respectively.The in vivo experiment demonstrates enhanced bone tissues in the strut-based scaffold.This study significantly enriches our understanding of TPMS structures and provides significant insights in the design of bone scaffolds under various bone damaging conditions. 展开更多
关键词 Triply periodic minimal surface Bone repair Tissue engineering Mechanical properties Laser powder bed fusion
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Robotic arm-assisted arthroplasty:The latest developments
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作者 Xin Chen Shu Deng +1 位作者 Mao-Lin Sun Rui He 《Chinese Journal of Traumatology》 CAS CSCD 2022年第3期125-131,共7页
Joint arthroplasty is an effective method for treating end-stage joint lesions and damages.Robotic arm-assisted arthroplasty,a rapidly developing technology that combines navigation technology,minimally invasive techn... Joint arthroplasty is an effective method for treating end-stage joint lesions and damages.Robotic arm-assisted arthroplasty,a rapidly developing technology that combines navigation technology,minimally invasive technology,and precise control technology of the robotic arm,can achieve accurate preoperative planning,optimal selection of implants,minimally invasive surgery,precise osteotomy,and accurate placement of the artificial joint.It has the characteristics of high accuracy and stability,and thus is more and more widely used in the field of joint surgery.In this paper,we systematically reviewed the application and clinical efficacy of robotic arm-assisted technology in hip and knee arthroplasty to provide reference for its future promotion. 展开更多
关键词 Robotic arm ARTHROPLASTY HIP KNEE Clinical efficacy
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