BACKGROUND Sessile serrated lesions(SSLs)are considered precancerous colorectal lesions that should be detected and removed to prevent colorectal cancer.Previous studies in Vietnam mainly investigated the adenoma path...BACKGROUND Sessile serrated lesions(SSLs)are considered precancerous colorectal lesions that should be detected and removed to prevent colorectal cancer.Previous studies in Vietnam mainly investigated the adenoma pathway,with limited data on the serrated pathway.AIM To evaluate the prevalence,risk factors,and BRAF mutations of SSLs in the Vietnamese population.METHODS This is a cross-sectional study conducted on patients with lower gastrointestinal symptoms who underwent colonoscopy at a tertiary hospital in Vietnam.SSLs were diagnosed on histopathology according to the 2019 World Health Organi-zation classification.BRAF mutation analysis was performed using the Sanger DNA sequencing method.The multivariate logistic regression model was used to determine SSL-associated factors.RESULTS There were 2489 patients,with a mean age of 52.1±13.1 and a female-to-male ratio of 1:1.1.The prevalence of SSLs was 4.2%[95%confidence interval(CI):3.5-5.1].In the multivariate analysis,factors significantly associated with SSLs were age≥40[odds ratio(OR):3.303;95%CI:1.607-6.790],male sex(OR:2.032;95%CI:1.204-3.429),diabetes mellitus(OR:2.721;95%CI:1.551-4.772),and hypertension(OR:1.650,95%CI:1.045-2.605).The rate of BRAF mutations in SSLs was 35.5%.CONCLUSION The prevalence of SSLs was 4.2%.BRAF mutations were present in one-third of SSLs.Significant risk factors for SSLs included age≥40,male sex,diabetes mellitus,and hypertension.展开更多
Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effect...Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effects of EGCG on eell growth of IMS-M2 cells harboring the NPM1 mutations.Western blot analysis were performed to test the protein expression of NPM1,AKT,those associated with apoplosis.Results:EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1mutations.Moreover,EGCG also suppressed the cell proliferation and induced apoptosis in IMSM2 cells.Conclusions:The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.展开更多
文摘BACKGROUND Sessile serrated lesions(SSLs)are considered precancerous colorectal lesions that should be detected and removed to prevent colorectal cancer.Previous studies in Vietnam mainly investigated the adenoma pathway,with limited data on the serrated pathway.AIM To evaluate the prevalence,risk factors,and BRAF mutations of SSLs in the Vietnamese population.METHODS This is a cross-sectional study conducted on patients with lower gastrointestinal symptoms who underwent colonoscopy at a tertiary hospital in Vietnam.SSLs were diagnosed on histopathology according to the 2019 World Health Organi-zation classification.BRAF mutation analysis was performed using the Sanger DNA sequencing method.The multivariate logistic regression model was used to determine SSL-associated factors.RESULTS There were 2489 patients,with a mean age of 52.1±13.1 and a female-to-male ratio of 1:1.1.The prevalence of SSLs was 4.2%[95%confidence interval(CI):3.5-5.1].In the multivariate analysis,factors significantly associated with SSLs were age≥40[odds ratio(OR):3.303;95%CI:1.607-6.790],male sex(OR:2.032;95%CI:1.204-3.429),diabetes mellitus(OR:2.721;95%CI:1.551-4.772),and hypertension(OR:1.650,95%CI:1.045-2.605).The rate of BRAF mutations in SSLs was 35.5%.CONCLUSION The prevalence of SSLs was 4.2%.BRAF mutations were present in one-third of SSLs.Significant risk factors for SSLs included age≥40,male sex,diabetes mellitus,and hypertension.
基金Supported by the Japan Foundation for Promotion of International Medical Research Co-operation(JF-PIMRC)
文摘Objective:To investigate the inhibited effect of epigallocatechin-3-gallate(EGCG)on the expression of NPM1 in IMS-M2 cells harboring the NPMl mutations.Methods:Cell proliferation assay was performed to test the effects of EGCG on eell growth of IMS-M2 cells harboring the NPM1 mutations.Western blot analysis were performed to test the protein expression of NPM1,AKT,those associated with apoplosis.Results:EGCG can down-regulate the expression of NPM1 in IMS-M2 cells harboring the NPM1mutations.Moreover,EGCG also suppressed the cell proliferation and induced apoptosis in IMSM2 cells.Conclusions:The results suggested that EGCG could be considered as a reagent for treatment of AML patients with NPM1 mutations.
