The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles...The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles in hepatocarcinogenesis still need to be elucidated.Many tumor suppressor genes(TSGs)have been identified as being involved in HCC.These TSGs can be classified into two groups depending on the situation with respect to allelic mutation/loss in the tumors:the recessive TSGs with two required mutated alleles and the haploinsufficient TSGs with one required mutated allele.Hepatitis B virus(HBV)is one of the most important risk factors associated with HCC.Although mice cannot be infected with HBV due to the narrow host range of HBV and the lack of a proper receptor,one advantage of mouse models for HBV/HCC research is the numerous and powerfulgenetic tools that help investigate the phenotypic effects of viral proteins and allow the dissection of the dose-dependent action of TSGs.Here,we mainly focus on the application of mouse models in relation to HBV-associated HCC and on TSGs that act either in a recessive or in a haploinsufficient manner.Discoveries obtained using mouse models will have a great impact on HCC translational medicine.展开更多
OBJECTIVE:Tostudy the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell lineA549.METHODS: N-acetyltransferase antibodies were prepared. The human lung cancer A549 ce...OBJECTIVE:Tostudy the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell lineA549.METHODS: N-acetyltransferase antibodies were prepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate,and divided into the control group, and berberine0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups,with 3 wells for each group.24h later,A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse transcription polymerase chain reaction.RESULTS: The N-acetyltransferase content in human lung cancer A549 cells decreased with the increasing of berberine concentration, significantly lower than that in the control group(P<0.05 or P<0.001); and the mRNA expression of N-acetyltransferase also decreased with the increasing of berberine concentration, significantly lower in Huangliansu treatment groups(P<0.001).CONCLUSION: Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent.The inhibited gene expression of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mechanisms for itsantineoplastic effect.展开更多
基金Supported by Research grants from the Ministry of Science and Technology(MOST)in Taiwan,No.NSC99-2628-B-010-001-MY3,MOST 103-2321-B-010-003,MOST 103-2633-H-010-001,MOST 103-2633-B-400-002 and MOST104-3011-B-010-001a grant from the Ministry of Education,Aim for the Top University Plan
文摘The multifactorial and multistage pathogenesis of hepatocellular carcinoma(HCC)has fascinated a wide spectrum of scientists for decades.While a number of major risk factors have been identified,their mechanistic roles in hepatocarcinogenesis still need to be elucidated.Many tumor suppressor genes(TSGs)have been identified as being involved in HCC.These TSGs can be classified into two groups depending on the situation with respect to allelic mutation/loss in the tumors:the recessive TSGs with two required mutated alleles and the haploinsufficient TSGs with one required mutated allele.Hepatitis B virus(HBV)is one of the most important risk factors associated with HCC.Although mice cannot be infected with HBV due to the narrow host range of HBV and the lack of a proper receptor,one advantage of mouse models for HBV/HCC research is the numerous and powerfulgenetic tools that help investigate the phenotypic effects of viral proteins and allow the dissection of the dose-dependent action of TSGs.Here,we mainly focus on the application of mouse models in relation to HBV-associated HCC and on TSGs that act either in a recessive or in a haploinsufficient manner.Discoveries obtained using mouse models will have a great impact on HCC translational medicine.
基金Supported by Xiamen Science and Technology Key Program Plan Grant(No.3502Z20100006)Scientific Research Starting Foundation for New Teacher of Xiamen University(No.ZK1014)
文摘OBJECTIVE:Tostudy the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell lineA549.METHODS: N-acetyltransferase antibodies were prepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate,and divided into the control group, and berberine0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups,with 3 wells for each group.24h later,A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse transcription polymerase chain reaction.RESULTS: The N-acetyltransferase content in human lung cancer A549 cells decreased with the increasing of berberine concentration, significantly lower than that in the control group(P<0.05 or P<0.001); and the mRNA expression of N-acetyltransferase also decreased with the increasing of berberine concentration, significantly lower in Huangliansu treatment groups(P<0.001).CONCLUSION: Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent.The inhibited gene expression of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mechanisms for itsantineoplastic effect.
