Objective Hypertriglyceridemic waist(HW),hypertriglyceridemic waist-to-height ratio(HWHtR),and waist-to-hip ratio(WHR)have been shown to be indicators of cardiometabolic risk factors.However,it is not clear which indi...Objective Hypertriglyceridemic waist(HW),hypertriglyceridemic waist-to-height ratio(HWHtR),and waist-to-hip ratio(WHR)have been shown to be indicators of cardiometabolic risk factors.However,it is not clear which indicator is more suitable for children and adolescents.We aimed to investigate the relationship between HW,HWHtR,WHR,and cardiovascular risk factors clustering to determine the best screening tools for cardiometabolic risk in children and adolescents.Methods This was a national cross-sectional study.Anthropometric and biochemical variables were assessed in approximately 70,000 participants aged 6–18 years from seven provinces in China.Demographics,physical activity,dietary intake,and family history of chronic diseases were obtained through questionnaires.ANOVA,χ2 and logistic regression analysis was conducted.Results A significant sex difference was observed for HWHtR and WHR,but not for HW phenotype.The risk of cardiometabolic health risk factor clustering with HW phenotype or the HWHtR phenotype was significantly higher than that with the non-HW or non-HWHtR phenotypes among children and adolescents(HW:OR=12.22,95%CI:9.54-15.67;HWHtR:OR=9.70,95%CI:6.93-13.58).Compared with the HW and HWHtR phenotypes,the association between risk of cardiometabolic health risk factors(CHRF)clustering and high WHR was much weaker and not significant(WHR:OR=1.14,95%CI:0.97-1.34).Conclusion Compared with HWHtR and WHR,the HW phenotype is a more convenient indicator with higher applicability to screen children and adolescents for cardiovascular risk factors.展开更多
Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from Septemb...Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from September 2016 to September 2018 were divided into chronic persistence group, remission group and acute attack group. Thirty healthy children who underwent physical examination in our hospital were selected and divided into control group. The levels of ECP, 25-(OH) D3 and M2 receptors were analyzed by ELISA, and Pearson correlation analysis was performed.Results: Compared with the control group, the levels of ECP and M2 receptors in chronic persistence group, remission group and acute attack group increased, while the levels of 25-(OH)D3 decreased, with statistical difference (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in chronic persistence group, and the levels of 25-(OH)D3 were lower than those in chronic persistence group (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in remission group, and the levels of 25-(OH)D3 were lower than those in remission group (P<0.05). Compared with mild children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in moderate and severe children (P<0.05). Compared with moderate children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in severe children (P<0.05).There was a negative correlation between ECP and 25-(OH)D3 (r=-0.380, P=0.038);a negative correlation between 25-(OH)D3 and M2 receptor (r=-0.448,P=0.013);and a positive correlation between ECP and M2 receptor (r=0.450,P=0.013).Conclusions:The expression of ECP and M2 receptors increased during the acute attack of bronchial asthma in children, while the expression of 25-hydroxyvitamin D3 decreased during the acute attack of bronchial asthma in children. The correlation among ECP, 25-(OH) D3 and M2 receptors is significant in the clinical diagnosis of acute attack of bronchial asthma in children.展开更多
Objective： To observe the efficacy of electroacupuncture plus TDP for treating functional dyspepsia Methods： Sixty cases of functional dyspepsia were treated with electroacupuncture plus TDP, and another 60 cases we...Objective： To observe the efficacy of electroacupuncture plus TDP for treating functional dyspepsia Methods： Sixty cases of functional dyspepsia were treated with electroacupuncture plus TDP, and another 60 cases were treated with Domperidone as a control. Results： After 10 treatments, the curative rate in treatment group was 33.3%, with a total effective rate of 91.7%. While in control group, the curative rate was 13.3%, with a total effective rate of 66.7%. There were significant differences between the two groups （P〈0.01, P〈0.001）. Conclusion： Electroacupuncture plus TDP had the better therapeutic effect in treating functional dyspepsia.展开更多
Objective To investigate the effect of over-expression of E2 protein GFP-E2,GFP-TAD(N-extremity domain of HPV18 E2)and GFP-DBD(C-extremity domain of HPV18 E2)fusion proteins on the apoptosis of macrophages in uterine ...Objective To investigate the effect of over-expression of E2 protein GFP-E2,GFP-TAD(N-extremity domain of HPV18 E2)and GFP-DBD(C-extremity domain of HPV18 E2)fusion proteins on the apoptosis of macrophages in uterine cervix cancer was studied.Methods TAD or DBD gene was amplified by PCR from pEGFP-C1/HPV18 E2,and cloned into pEGFP-C1 vector.Then the subclone of pEGFP-C1/TAD or pEGFP-C1/DBD was screened respectively.Forty-eight hours after transfection of pEGFP-C1/HPV18 E2,pEGFP-C1/TAD,pEGFP-C1/DBD or pEGFP-C1 into macrophages,the localization or expression of them was analyzed by fluorescent microscope or western blot,respectively.The apoptotic rate of macrophages was detected by flow cytometry.Results The plasmid of pEGFP-C1/TAD or pEGFP-C1/ DBD was constructed respectively.Macrophages transfected with different plasmid,over-expression of GFP-E2 or GFP-TAD fusion protein up-regulated apoptotic rate of macrophages.Furthermore,the effect of GFP -TAD was more powerful than GFP-E2.But the GFP-DBD over-expressed had not the same effects.Conclusion Over-expression of GFP-E2 or GFP-TAD fusion protein could induce apoptosis of macrophages.展开更多
基金supported by the National Natural Science Foundation of China[no.81903336,Yi-de Yang]the Health Research Project of Hunan Provincial Health Commission[no.202112031516,Yi-de Yang]+3 种基金Scientific Research Fund of Hunan Provincial Education Department[no.22B0038,Yi-de Yang]the Research Team for Reproduction Health and Translational Medicine of Hunan Normal University[2023JC101]Key Project of Developmental Biology and Breeding from Hunan Province[no.2022XKQ0205]Open Project for Postgraduates of Hunan Normal University[no.KF2022019,Tianli Xiao].
文摘Objective Hypertriglyceridemic waist(HW),hypertriglyceridemic waist-to-height ratio(HWHtR),and waist-to-hip ratio(WHR)have been shown to be indicators of cardiometabolic risk factors.However,it is not clear which indicator is more suitable for children and adolescents.We aimed to investigate the relationship between HW,HWHtR,WHR,and cardiovascular risk factors clustering to determine the best screening tools for cardiometabolic risk in children and adolescents.Methods This was a national cross-sectional study.Anthropometric and biochemical variables were assessed in approximately 70,000 participants aged 6–18 years from seven provinces in China.Demographics,physical activity,dietary intake,and family history of chronic diseases were obtained through questionnaires.ANOVA,χ2 and logistic regression analysis was conducted.Results A significant sex difference was observed for HWHtR and WHR,but not for HW phenotype.The risk of cardiometabolic health risk factor clustering with HW phenotype or the HWHtR phenotype was significantly higher than that with the non-HW or non-HWHtR phenotypes among children and adolescents(HW:OR=12.22,95%CI:9.54-15.67;HWHtR:OR=9.70,95%CI:6.93-13.58).Compared with the HW and HWHtR phenotypes,the association between risk of cardiometabolic health risk factors(CHRF)clustering and high WHR was much weaker and not significant(WHR:OR=1.14,95%CI:0.97-1.34).Conclusion Compared with HWHtR and WHR,the HW phenotype is a more convenient indicator with higher applicability to screen children and adolescents for cardiovascular risk factors.
基金Key projects funded by the science and technology bureau of Chenzhou city(No.CZ2013065).
文摘Objective:To study the expression and significance of ECP, 25-(OH)D3 and M2 receptors in children with acute attack of asthma.Methods: Seventy children with bronchial asthma who first visited our hospital from September 2016 to September 2018 were divided into chronic persistence group, remission group and acute attack group. Thirty healthy children who underwent physical examination in our hospital were selected and divided into control group. The levels of ECP, 25-(OH) D3 and M2 receptors were analyzed by ELISA, and Pearson correlation analysis was performed.Results: Compared with the control group, the levels of ECP and M2 receptors in chronic persistence group, remission group and acute attack group increased, while the levels of 25-(OH)D3 decreased, with statistical difference (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in chronic persistence group, and the levels of 25-(OH)D3 were lower than those in chronic persistence group (P<0.05). The levels of ECP and M2 receptors in acute attack group were higher than those in remission group, and the levels of 25-(OH)D3 were lower than those in remission group (P<0.05). Compared with mild children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in moderate and severe children (P<0.05). Compared with moderate children, the levels of ECP and M2 receptors increased and 25-(OH)D3 decreased in severe children (P<0.05).There was a negative correlation between ECP and 25-(OH)D3 (r=-0.380, P=0.038);a negative correlation between 25-(OH)D3 and M2 receptor (r=-0.448,P=0.013);and a positive correlation between ECP and M2 receptor (r=0.450,P=0.013).Conclusions:The expression of ECP and M2 receptors increased during the acute attack of bronchial asthma in children, while the expression of 25-hydroxyvitamin D3 decreased during the acute attack of bronchial asthma in children. The correlation among ECP, 25-(OH) D3 and M2 receptors is significant in the clinical diagnosis of acute attack of bronchial asthma in children.
文摘Objective： To observe the efficacy of electroacupuncture plus TDP for treating functional dyspepsia Methods： Sixty cases of functional dyspepsia were treated with electroacupuncture plus TDP, and another 60 cases were treated with Domperidone as a control. Results： After 10 treatments, the curative rate in treatment group was 33.3%, with a total effective rate of 91.7%. While in control group, the curative rate was 13.3%, with a total effective rate of 66.7%. There were significant differences between the two groups （P〈0.01, P〈0.001）. Conclusion： Electroacupuncture plus TDP had the better therapeutic effect in treating functional dyspepsia.
基金supported by the Edueation Committee of Hunan province(No.02C391)
文摘Objective To investigate the effect of over-expression of E2 protein GFP-E2,GFP-TAD(N-extremity domain of HPV18 E2)and GFP-DBD(C-extremity domain of HPV18 E2)fusion proteins on the apoptosis of macrophages in uterine cervix cancer was studied.Methods TAD or DBD gene was amplified by PCR from pEGFP-C1/HPV18 E2,and cloned into pEGFP-C1 vector.Then the subclone of pEGFP-C1/TAD or pEGFP-C1/DBD was screened respectively.Forty-eight hours after transfection of pEGFP-C1/HPV18 E2,pEGFP-C1/TAD,pEGFP-C1/DBD or pEGFP-C1 into macrophages,the localization or expression of them was analyzed by fluorescent microscope or western blot,respectively.The apoptotic rate of macrophages was detected by flow cytometry.Results The plasmid of pEGFP-C1/TAD or pEGFP-C1/ DBD was constructed respectively.Macrophages transfected with different plasmid,over-expression of GFP-E2 or GFP-TAD fusion protein up-regulated apoptotic rate of macrophages.Furthermore,the effect of GFP -TAD was more powerful than GFP-E2.But the GFP-DBD over-expressed had not the same effects.Conclusion Over-expression of GFP-E2 or GFP-TAD fusion protein could induce apoptosis of macrophages.
