This paper collected and arranged competitive scientific research projects undertaken by Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences in 2003-2014. Through statistica...This paper collected and arranged competitive scientific research projects undertaken by Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences in 2003-2014. Through statistical analysis on quantity of projects,funded amount,age of person responsible,professional title of person responsible,academic degree of person responsible,research object,it discussed relevant characteristics and rules. Finally,it came up with pertinent measures and recommendations,in the hope of providing services for decision-making and scientific and technological management.展开更多
Taking Tropical Crops Genetic Resources Institute( TCGRI) of Chinese Academy of Tropical Agricultural Sciences( CATAS) as an example,this paper discussed current situation of construction of sci-tech platform,analyzed...Taking Tropical Crops Genetic Resources Institute( TCGRI) of Chinese Academy of Tropical Agricultural Sciences( CATAS) as an example,this paper discussed current situation of construction of sci-tech platform,analyzed existing problems,and finally came up with pertinent recommendations.展开更多
The article introduces the main practices and achievements of the Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences in promoting the sharing of large-scale instruments and...The article introduces the main practices and achievements of the Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences in promoting the sharing of large-scale instruments and equipment in recent years,analyzes the existing problems in the management system,management team,assessment incentives and maintenance guarantee,and proposes improvement measures and suggestions from aspects of improving the sharing management system,strengthening management team building,strengthening sharing assessment and incentives,improving maintenance capabilities and expanding external publicity,to further improve the sharing management of large-scale instruments and equipment.展开更多
Podostemaceae(riverweed family),one of the largest aquatic plant families in the world,comprises about 350 species in 51 genera placed in three subfamilies,Podostemoideae,Weddellinoideae,and Tristichoideae(Koi et al.,...Podostemaceae(riverweed family),one of the largest aquatic plant families in the world,comprises about 350 species in 51 genera placed in three subfamilies,Podostemoideae,Weddellinoideae,and Tristichoideae(Koi et al.,2022).Most Podostemaceae genera(~38)are monotypic or oligotypic,with a high level of endemism(Koi et al.,2018,2022).Members of Podostemaceae adhere to rocks in fast-flowing rivers,streams,or waterfalls in the subtropics and tropics(Koi et al.,2018).As an adaptation to fastrunning currents,the morphological structure of river-weeds tends to be extremely reduced,including a loss or reduction of primary shoots or roots(Koi and Kato,2007).The vegetative mass of these plants resembles lichens or bryophytes,and are thus known commonly as“dream bryophytes”(Philbrick and Retana,1998).Podostemaceae plants flower when the water level drops during the dry season.In recent years,anthropogenic factors and climate change have severely affected river weed habitats,decreasing populations of these plants.展开更多
Industrialization of tropical agricultural science and technology is an essential part of tropical agricultural technological innovation.This paper firstly analyzed development mode of industrialization of tropical ag...Industrialization of tropical agricultural science and technology is an essential part of tropical agricultural technological innovation.This paper firstly analyzed development mode of industrialization of tropical agricultural science and technology in Chin and then came up with recommendations for developing industrialization of tropical agricultural science and technology.展开更多
The Agricultural Science and Technology Experiment and Demonstration Park,as a unique tourist scenic spot,is a new model for the development of low-carbon leisure agriculture.In this paper,with Xinglong Tropical Botan...The Agricultural Science and Technology Experiment and Demonstration Park,as a unique tourist scenic spot,is a new model for the development of low-carbon leisure agriculture.In this paper,with Xinglong Tropical Botanical Park as a study case,the practice of developing a model of low-carbon agricultural science and technology tourism in the park is explored.Main measures for developing low-carbon leisure agriculture in Agricultural Science and Technology Experiment and Demonstration Park are summarized,including development of low carbon attractors,construction of low carbon facilities,strengthening low-carbon management,building low-carbon environment and so on,according to analysis on the models for development of low-carbon agricultural science tourism in this park.展开更多
The quality and safety crisis of agricultural products on the one hand is the crisis of harm of agricultural products,and on the other hand is the crisis of information dissemination. This paper analyzed the character...The quality and safety crisis of agricultural products on the one hand is the crisis of harm of agricultural products,and on the other hand is the crisis of information dissemination. This paper analyzed the characteristics of content of the quality and safety crisis information of tropical agricultural products. On the basis of these characteristics,it came up with strategies and recommendations for solving the problems of quality and safety of tropical agricultural products,including establishing the emergency response system for quality and safety crisis of tropical agricultural products,bringing into play the social responsibilities of scientific research institutions,and bringing into play authoritative effect of scientific experts.展开更多
Reforestation or natural forest regeneration is an alternative measure for controlling soil erosion in degraded land on the Chinese Loess Plateau(CLP). However, our understanding of the temporal dynamics and the spati...Reforestation or natural forest regeneration is an alternative measure for controlling soil erosion in degraded land on the Chinese Loess Plateau(CLP). However, our understanding of the temporal dynamics and the spatial patterns of forest regeneration remains inadequate. Two oak forests at different development stages were investigated to determine the spatial patterns of competitions(intraspecies and interspecies) during different successional stages. The intraspecies and interspecies spatial relationships among different tree diameters at breast height were analyzed at multiple scales by Kriging interpolation method and univariate and bivariate O-ring statistics. Our analytical results indicated that self-correlation and competition intensity were relatively high between oak and pine trees in the early development stage of oak forests due to their clumped distributions of heavy seeds. Birch trees had a lower competition in comparison to oak trees although birch was the dominant species. Therefore, asymmetric competition of oak trees was most likely to have led to their edge dispersal and their success in replacing the pioneer species. Asymmetric competition means that larger individuals obtained a disproportionately large share of the resources and suppressed the growth of smaller individuals. Kriging interpolation analysis showed a tendency towards homogenization caused by interspecies competition during the succession of oak forests. Our results demonstrated that the competition was the driving factor in the spatial distribution of oak forests on the CLP.展开更多
The transformation of scientific and technological achievements concerning tropical agriculture has the characteristics of high risk and high return. Based on the characteristics of transformation of scientific and te...The transformation of scientific and technological achievements concerning tropical agriculture has the characteristics of high risk and high return. Based on the characteristics of transformation of scientific and technological achievements concerning tropical agriculture,this paper analyzes the risk factors during the transformation of scientific and technological achievements concerning tropical agriculture. In accordance with the current situation of low transformation rate of scientific and technological achievements concerning tropical agriculture in China,this paper proposes the measures to avoid the risks in the transformation of scientific and technological achievements concerning tropical agriculture and enhance the transformation rate of scientific and technological achievements concerning tropical agriculture.展开更多
HYL1(HYPONASTIC LEAVES 1)in Arabidopsis thaliana encodes a double-stranded RNA-binding protein needed for proper miRNA maturation,and its null mutant hyl1 shows a typical leaf-incurvature phenotype.In Chinese cabbage,...HYL1(HYPONASTIC LEAVES 1)in Arabidopsis thaliana encodes a double-stranded RNA-binding protein needed for proper miRNA maturation,and its null mutant hyl1 shows a typical leaf-incurvature phenotype.In Chinese cabbage,BcpLH(Brassica rapa ssp.pekinensis LEAFY HEADS),a close homolog of HYL1,is differentially expressed in juvenile leaves,which are flat,and in adult leaves,which display extreme incurvature.BcpLH lacks protein–protein interaction domains and is much shorter than HYL1.To test whether BcpLH is associated with defects in microRNA(miRNA)biogenesis and leaf flatness,we enhanced and repressed the activity of BcpLH by transgenics and investigated BcpLH-dependent miRNAs and plant morphology.BcpLH promoted miRNA biogenesis by the proper processing of primary miRNAs.BcpLH downregulation via antisense decreased a specific subset of miRNAs and increased the activities of their target genes,causing upward curvature of rosette leaves and early leaf incurvature,concurrent with the enlargement,earliness,and round-to-oval shape transition of leafy heads.Moreover,BcpLH-dependent miRNAs in Chinese cabbage are not the same as HYL1-dependent miRNAs in Arabidopsis.We suggest that BcpLH controls a specific subset of miRNAs in Chinese cabbage and coordinates the direction,extent,and timing of leaf curvature during head formation in Brassica rapa.展开更多
Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical fruit.Since litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(su...Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical fruit.Since litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(such as hybridization)have limited ability to nurture new litchi cultivars.Here,an efficient and stable Agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in‘Feizixiao’litchi.Transgenic materials were verified using polymerase chain reaction(PCR)analysis,β-glucuronidase(GUS)assay,and green fluorescent protein(GFP)assay.To implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/associated protein 9(CRISPR/Cas9)technology in‘Feizixiao’litchi and verify the validity of these transformation systems,the litchi polyphenol oxidase gene(LcPPO,JF926153)was knocked out.Various categories of mutations,covering base insertions,deletions,and substitutions,were found in transgenic materials via sequence analysis.The transformation system achieved high feasibility and efficiency,and the system of CRISPR/Cas9 was successfully employed to edit genes in‘Feizixiao’litchi.This work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement.展开更多
Mango bacterial canker is caused by Xanthomonas campestris pv. mangiferaeindicae. During 2009 and 2013,leaves,twigs and fruits of mango were collected from commercial and experimental mango fields with typical canker ...Mango bacterial canker is caused by Xanthomonas campestris pv. mangiferaeindicae. During 2009 and 2013,leaves,twigs and fruits of mango were collected from commercial and experimental mango fields with typical canker symptoms in Hainan,Guangxi,Guangdong and Szechwan Provinces of China. The causal agent was identified as X. campestris pv. mangiferaeindicae through KC semi-selective medium isolation,pathogenicity tests,and sequencing of the gyrB gene.展开更多
[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the ba...[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the basis of the introduction of green-stem non-heading Chinese cabbage and dark-green-leaf non-heading Chinese cabbage varieties, the varieties tolerant to heat and humidity were identified by the investigation of agronomic traits and evaluation of heat injury index. [Results] The data analysis results showed that four green-stem non-heading Chinese cabbage varieties including Huaguan Qingcaigeng F_1, Zhongguan Qinggeng, Hanshen No. 1 Qinggengcai F_1 and Huaxiawang Piaocai have the characteristics of strong tolerance to heat and high yield, and four dark-green-leaf non-heading Chinese cabbage varieties including Baimeigui, Heijingang, Xiaxue Naibaicai and Siji Baicai have the characteristics of strong tolerance to heat and high yield. [Conclusions] These eight leaf vegetable varieties are suitable for popularization in summer and autumn in Hainan Province.展开更多
This paper firstly reviewed development process of UPOV Conventions and briefly introduced current situation of protection of new tropical crop varieties in China. Using SWOT approach,it analyzed internal strengths,we...This paper firstly reviewed development process of UPOV Conventions and briefly introduced current situation of protection of new tropical crop varieties in China. Using SWOT approach,it analyzed internal strengths,weaknesses,external opportunities and threats of breeding R&D of tropical crops if implementing UPOV Convention 1991 Act in China. Internal strengths include rich resources,strategic significance of tropical crops,solid crop breeding foundation,and broad market prospect. Internal weaknesses include insufficient reserve of germplasm resources,weak R&D and protection of new varieties,low market level of breeding R&D entities,unreasonable structure,low input in breeding R&D,and weak protection awareness. Opportunities include bringing into play strengths of rich tropical plant resources,stimulating enthusiasm for new variety R&D and awareness for variety protection,promoting structural optimization of breeding input entities,raising technology level of breeding R&D,and promoting international cooperation of new variety protection. Threats include more strict restriction of intellectual property right,impact to weak fields of new variety R&D,and difficulty in implementing limitation of farmer privileges. Finally,it came up with four recommendations for breeding R&D of tropical crops in China in the contest of implementing UPOV Convention 1991 Act.展开更多
At present,there is a contradiction between the rapid growth of the number of patents declared and authorized by agricultural scientific research institutions and the decline of the efficiency of invention patents yea...At present,there is a contradiction between the rapid growth of the number of patents declared and authorized by agricultural scientific research institutions and the decline of the efficiency of invention patents year by year,and the low conversion rate of patents.This paper discusses the patent application quantity,authorization quantity and patent efficiency of the Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,analyzes the present situation of its work and the main problems existing in patent management,and puts forward the relevant suggestions.展开更多
Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has ma...Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has made active exploration in building a modern agricultural system.China has established cooperation with the four largest agricultural research institutes in China to offer technical support for the development of modern agriculture.In this paper,the status quo and existing problems of agricultural development in Jiangmen,Guangdong are briefly introduced.Then,the cooperation status and prospect of local government with the research institutes are discussed.Finally some suggestions are given on the agricultural development pattern in Jiangmen City.展开更多
In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the manage...In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the management of special materials becomes more standardized.In order to do well in management of special materials,the authors made exploration and thinking about further strengthening management of special materials in combination with actual conditions,including establishing regulations and systems,enhancing procurement process,strengthening requisition and safekeeping,and reinforcing general ledger management of special materials.展开更多
Project management of agricultural scientific research institutions is an important section of agricultural scientific research plan management. It is of great significance for sustainable development of scientific re...Project management of agricultural scientific research institutions is an important section of agricultural scientific research plan management. It is of great significance for sustainable development of scientific research work of scientific research institutions. According to a series of opinions and notices about scientific and technological system reform issued by the state,and combining current situations of management of scientific research projects in scientific research institutions,this paper made a preliminary study on management of agricultural scientific research projects in the new trend. Finally,on the basis of the current situations of management of agricultural scientific research projects,it came up with pertinent recommendations,including strengthening communication and cooperation and actively declaring projects,strengthening preliminary planning of projects and establishing project information database,reinforcing project process management,ensuring on-time and high quality completion of projects,and strengthening learning and improving quality of management personnel.展开更多
Taking Environment and Plant Protection Institute in Chinese Academy of Tropical Agricultural Sciences as an example,it summarized major experience and practice in management of scientific research projects from stren...Taking Environment and Plant Protection Institute in Chinese Academy of Tropical Agricultural Sciences as an example,it summarized major experience and practice in management of scientific research projects from strengthening project database,enhancing project planning,reinforcing scientific research management team construction,bringing into play functions of academies and institutes,and improving management system. It analyzed existing problems and came up with recommendations from strengthening project process management,improving management team quality,and improving incentive mechanism,in the hope of providing reference for scientific research project management in agricultural scientific research institutes.展开更多
In recent years,the Internet of Things(IoT)technology has been widely used in the production and sales of tropical fruits,with strong practicability and wide application prospects.The tropical fruit dynamic pricing mo...In recent years,the Internet of Things(IoT)technology has been widely used in the production and sales of tropical fruits,with strong practicability and wide application prospects.The tropical fruit dynamic pricing model based on the IoT technology can promote the healthy development of the tropical fruit industry in Hainan and ensure the income of fruit farmers.Based on IoT technology,the quality grade of tropical fruits in Hainan is obtained.According to the dynamic pricing strategy of revenue management,a dynamic pricing model based on the quality of tropical fruits and a dynamic pricing model based on consumer segmentation are established to study the dynamic pricing problem under the condition of maximum profit for tropical fruit sellers.The research results show that for different fruit quality and consumer groups,different pricing models are required for pricing,in order to get the maximum profit from tropical fruit sales.Sellers must flexibly adopt different dynamic pricing models to price tropical fruits to enhance the competitiveness of the tropical fruit industry.展开更多
基金Supported by Special Project for Reform of Non-profit Scientific Research Institution"Study on Whole Process Management of Agricultural Scientific and Technological Projects"
文摘This paper collected and arranged competitive scientific research projects undertaken by Tropical Crops Genetic Resources Institute of Chinese Academy of Tropical Agricultural Sciences in 2003-2014. Through statistical analysis on quantity of projects,funded amount,age of person responsible,professional title of person responsible,academic degree of person responsible,research object,it discussed relevant characteristics and rules. Finally,it came up with pertinent measures and recommendations,in the hope of providing services for decision-making and scientific and technological management.
基金Supported by Fund on Basic Scientific Research Project of Nonprofit Central Research Institutions(1630032014023)
文摘Taking Tropical Crops Genetic Resources Institute( TCGRI) of Chinese Academy of Tropical Agricultural Sciences( CATAS) as an example,this paper discussed current situation of construction of sci-tech platform,analyzed existing problems,and finally came up with pertinent recommendations.
文摘The article introduces the main practices and achievements of the Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences in promoting the sharing of large-scale instruments and equipment in recent years,analyzes the existing problems in the management system,management team,assessment incentives and maintenance guarantee,and proposes improvement measures and suggestions from aspects of improving the sharing management system,strengthening management team building,strengthening sharing assessment and incentives,improving maintenance capabilities and expanding external publicity,to further improve the sharing management of large-scale instruments and equipment.
基金supported by grants from the National Natural Science Foundation of China(Nos.32100186 and 32300182)the Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection(Guangxi Normal University),Ministry of Education,China(No.ERESEP2022K03).
文摘Podostemaceae(riverweed family),one of the largest aquatic plant families in the world,comprises about 350 species in 51 genera placed in three subfamilies,Podostemoideae,Weddellinoideae,and Tristichoideae(Koi et al.,2022).Most Podostemaceae genera(~38)are monotypic or oligotypic,with a high level of endemism(Koi et al.,2018,2022).Members of Podostemaceae adhere to rocks in fast-flowing rivers,streams,or waterfalls in the subtropics and tropics(Koi et al.,2018).As an adaptation to fastrunning currents,the morphological structure of river-weeds tends to be extremely reduced,including a loss or reduction of primary shoots or roots(Koi and Kato,2007).The vegetative mass of these plants resembles lichens or bryophytes,and are thus known commonly as“dream bryophytes”(Philbrick and Retana,1998).Podostemaceae plants flower when the water level drops during the dry season.In recent years,anthropogenic factors and climate change have severely affected river weed habitats,decreasing populations of these plants.
文摘Industrialization of tropical agricultural science and technology is an essential part of tropical agricultural technological innovation.This paper firstly analyzed development mode of industrialization of tropical agricultural science and technology in Chin and then came up with recommendations for developing industrialization of tropical agricultural science and technology.
基金Supported by Special Project for Spark Industrial Zone of Hainan Province(HNXH201104)
文摘The Agricultural Science and Technology Experiment and Demonstration Park,as a unique tourist scenic spot,is a new model for the development of low-carbon leisure agriculture.In this paper,with Xinglong Tropical Botanical Park as a study case,the practice of developing a model of low-carbon agricultural science and technology tourism in the park is explored.Main measures for developing low-carbon leisure agriculture in Agricultural Science and Technology Experiment and Demonstration Park are summarized,including development of low carbon attractors,construction of low carbon facilities,strengthening low-carbon management,building low-carbon environment and so on,according to analysis on the models for development of low-carbon agricultural science tourism in this park.
基金Supported by Special Project of Fundamental Research Funds for Central Public Welfare Research Institutes in Chinese Academy of Tropical Agricultural Sciences(1630012015021)Project of Natural Science Foundation of Hainan Province(714282)
文摘The quality and safety crisis of agricultural products on the one hand is the crisis of harm of agricultural products,and on the other hand is the crisis of information dissemination. This paper analyzed the characteristics of content of the quality and safety crisis information of tropical agricultural products. On the basis of these characteristics,it came up with strategies and recommendations for solving the problems of quality and safety of tropical agricultural products,including establishing the emergency response system for quality and safety crisis of tropical agricultural products,bringing into play the social responsibilities of scientific research institutions,and bringing into play authoritative effect of scientific experts.
基金funded by the National Natural Science Foundation of China (41301601)the Special Fund for Forest Scientific Research in the Public Interest (201304312)
文摘Reforestation or natural forest regeneration is an alternative measure for controlling soil erosion in degraded land on the Chinese Loess Plateau(CLP). However, our understanding of the temporal dynamics and the spatial patterns of forest regeneration remains inadequate. Two oak forests at different development stages were investigated to determine the spatial patterns of competitions(intraspecies and interspecies) during different successional stages. The intraspecies and interspecies spatial relationships among different tree diameters at breast height were analyzed at multiple scales by Kriging interpolation method and univariate and bivariate O-ring statistics. Our analytical results indicated that self-correlation and competition intensity were relatively high between oak and pine trees in the early development stage of oak forests due to their clumped distributions of heavy seeds. Birch trees had a lower competition in comparison to oak trees although birch was the dominant species. Therefore, asymmetric competition of oak trees was most likely to have led to their edge dispersal and their success in replacing the pioneer species. Asymmetric competition means that larger individuals obtained a disproportionately large share of the resources and suppressed the growth of smaller individuals. Kriging interpolation analysis showed a tendency towards homogenization caused by interspecies competition during the succession of oak forests. Our results demonstrated that the competition was the driving factor in the spatial distribution of oak forests on the CLP.
基金Supported by Fundamental Research Funds for Central Nonprofit Scientific Research Institutions(1630012015011)
文摘The transformation of scientific and technological achievements concerning tropical agriculture has the characteristics of high risk and high return. Based on the characteristics of transformation of scientific and technological achievements concerning tropical agriculture,this paper analyzes the risk factors during the transformation of scientific and technological achievements concerning tropical agriculture. In accordance with the current situation of low transformation rate of scientific and technological achievements concerning tropical agriculture in China,this paper proposes the measures to avoid the risks in the transformation of scientific and technological achievements concerning tropical agriculture and enhance the transformation rate of scientific and technological achievements concerning tropical agriculture.
基金supported by grants from the National Key Research and Development Program of China(Grant No.2016YFD0101900,2016YFD0100500)the Natural Science Foundation of China(Grant Nos.31771442 and 31571261).
文摘HYL1(HYPONASTIC LEAVES 1)in Arabidopsis thaliana encodes a double-stranded RNA-binding protein needed for proper miRNA maturation,and its null mutant hyl1 shows a typical leaf-incurvature phenotype.In Chinese cabbage,BcpLH(Brassica rapa ssp.pekinensis LEAFY HEADS),a close homolog of HYL1,is differentially expressed in juvenile leaves,which are flat,and in adult leaves,which display extreme incurvature.BcpLH lacks protein–protein interaction domains and is much shorter than HYL1.To test whether BcpLH is associated with defects in microRNA(miRNA)biogenesis and leaf flatness,we enhanced and repressed the activity of BcpLH by transgenics and investigated BcpLH-dependent miRNAs and plant morphology.BcpLH promoted miRNA biogenesis by the proper processing of primary miRNAs.BcpLH downregulation via antisense decreased a specific subset of miRNAs and increased the activities of their target genes,causing upward curvature of rosette leaves and early leaf incurvature,concurrent with the enlargement,earliness,and round-to-oval shape transition of leafy heads.Moreover,BcpLH-dependent miRNAs in Chinese cabbage are not the same as HYL1-dependent miRNAs in Arabidopsis.We suggest that BcpLH controls a specific subset of miRNAs in Chinese cabbage and coordinates the direction,extent,and timing of leaf curvature during head formation in Brassica rapa.
基金supported by grants from the National Key R&D Program of China(Grant No.2019YFD1000900)the Hainan Province Science and Technology Special Fund(Grant No.ZDYF2022XDNY253)the earmarked fund for CARS(Grant No.CARS-32-01)。
文摘Litchi(Litchi chinensis Sonn.)is a type of commercially prevalent subtropical and tropical fruit.Since litchi has a highly heterozygous genetic background and a long reproductive cycle,conventional breeding methods(such as hybridization)have limited ability to nurture new litchi cultivars.Here,an efficient and stable Agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in‘Feizixiao’litchi.Transgenic materials were verified using polymerase chain reaction(PCR)analysis,β-glucuronidase(GUS)assay,and green fluorescent protein(GFP)assay.To implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)/associated protein 9(CRISPR/Cas9)technology in‘Feizixiao’litchi and verify the validity of these transformation systems,the litchi polyphenol oxidase gene(LcPPO,JF926153)was knocked out.Various categories of mutations,covering base insertions,deletions,and substitutions,were found in transgenic materials via sequence analysis.The transformation system achieved high feasibility and efficiency,and the system of CRISPR/Cas9 was successfully employed to edit genes in‘Feizixiao’litchi.This work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement.
基金Supported by the Ministry of Science and Technology and the Ministry of Agriculture of China(2014hzs1J007-2)
文摘Mango bacterial canker is caused by Xanthomonas campestris pv. mangiferaeindicae. During 2009 and 2013,leaves,twigs and fruits of mango were collected from commercial and experimental mango fields with typical canker symptoms in Hainan,Guangxi,Guangdong and Szechwan Provinces of China. The causal agent was identified as X. campestris pv. mangiferaeindicae through KC semi-selective medium isolation,pathogenicity tests,and sequencing of the gyrB gene.
基金Supported by Major Science and Technology Project of Hainan Province(ZDKJ2017001)
文摘[Objectives] Introducing and screening leaf vegetable varieties tolerant to heat and humidity has great significance to ensure balanced supply of vegetables in summer and autumn in Hainan Province. [Methods] On the basis of the introduction of green-stem non-heading Chinese cabbage and dark-green-leaf non-heading Chinese cabbage varieties, the varieties tolerant to heat and humidity were identified by the investigation of agronomic traits and evaluation of heat injury index. [Results] The data analysis results showed that four green-stem non-heading Chinese cabbage varieties including Huaguan Qingcaigeng F_1, Zhongguan Qinggeng, Hanshen No. 1 Qinggengcai F_1 and Huaxiawang Piaocai have the characteristics of strong tolerance to heat and high yield, and four dark-green-leaf non-heading Chinese cabbage varieties including Baimeigui, Heijingang, Xiaxue Naibaicai and Siji Baicai have the characteristics of strong tolerance to heat and high yield. [Conclusions] These eight leaf vegetable varieties are suitable for popularization in summer and autumn in Hainan Province.
基金Supported by Special Fund for Basic Scientific Research Project in Chinese Academy of Tropical Agricultural Sciences(1630012014020)
文摘This paper firstly reviewed development process of UPOV Conventions and briefly introduced current situation of protection of new tropical crop varieties in China. Using SWOT approach,it analyzed internal strengths,weaknesses,external opportunities and threats of breeding R&D of tropical crops if implementing UPOV Convention 1991 Act in China. Internal strengths include rich resources,strategic significance of tropical crops,solid crop breeding foundation,and broad market prospect. Internal weaknesses include insufficient reserve of germplasm resources,weak R&D and protection of new varieties,low market level of breeding R&D entities,unreasonable structure,low input in breeding R&D,and weak protection awareness. Opportunities include bringing into play strengths of rich tropical plant resources,stimulating enthusiasm for new variety R&D and awareness for variety protection,promoting structural optimization of breeding input entities,raising technology level of breeding R&D,and promoting international cooperation of new variety protection. Threats include more strict restriction of intellectual property right,impact to weak fields of new variety R&D,and difficulty in implementing limitation of farmer privileges. Finally,it came up with four recommendations for breeding R&D of tropical crops in China in the contest of implementing UPOV Convention 1991 Act.
文摘At present,there is a contradiction between the rapid growth of the number of patents declared and authorized by agricultural scientific research institutions and the decline of the efficiency of invention patents year by year,and the low conversion rate of patents.This paper discusses the patent application quantity,authorization quantity and patent efficiency of the Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,analyzes the present situation of its work and the main problems existing in patent management,and puts forward the relevant suggestions.
基金Supported by the Special Fund for the Construction of Modern Agricultural Technical System(CARS-12-gdqxd)the Special Foundation for the Basic Scientific Research of Central Public Welfare Institute(1630032013033)
文摘Guangdong Province is one of the provinces in China with the best modern agricultural industry and the highest production efficiency.While Jiangmen City,one of the agriculture-based cities in Guangdong Province,has made active exploration in building a modern agricultural system.China has established cooperation with the four largest agricultural research institutes in China to offer technical support for the development of modern agriculture.In this paper,the status quo and existing problems of agricultural development in Jiangmen,Guangdong are briefly introduced.Then,the cooperation status and prospect of local government with the research institutes are discussed.Finally some suggestions are given on the agricultural development pattern in Jiangmen City.
文摘In recent years,the Ministry of Agriculture has increased input in agricultural scientific research projects.As a result,the procurement amount and quantity of special materials are increasing gradually,and the management of special materials becomes more standardized.In order to do well in management of special materials,the authors made exploration and thinking about further strengthening management of special materials in combination with actual conditions,including establishing regulations and systems,enhancing procurement process,strengthening requisition and safekeeping,and reinforcing general ledger management of special materials.
基金Supported by Special Project for Reform of Non-profit Scientific Research Institution"Study on Whole Process Management of Agricultural Scientific and Technological Projects"
文摘Project management of agricultural scientific research institutions is an important section of agricultural scientific research plan management. It is of great significance for sustainable development of scientific research work of scientific research institutions. According to a series of opinions and notices about scientific and technological system reform issued by the state,and combining current situations of management of scientific research projects in scientific research institutions,this paper made a preliminary study on management of agricultural scientific research projects in the new trend. Finally,on the basis of the current situations of management of agricultural scientific research projects,it came up with pertinent recommendations,including strengthening communication and cooperation and actively declaring projects,strengthening preliminary planning of projects and establishing project information database,reinforcing project process management,ensuring on-time and high quality completion of projects,and strengthening learning and improving quality of management personnel.
基金Supported Special Project for Fundamental Research Funds of Environment and Plant Protection Institute of Chinese Academy of Tropical Agricultural Sciences(No.2011hzs1J009)
文摘Taking Environment and Plant Protection Institute in Chinese Academy of Tropical Agricultural Sciences as an example,it summarized major experience and practice in management of scientific research projects from strengthening project database,enhancing project planning,reinforcing scientific research management team construction,bringing into play functions of academies and institutes,and improving management system. It analyzed existing problems and came up with recommendations from strengthening project process management,improving management team quality,and improving incentive mechanism,in the hope of providing reference for scientific research project management in agricultural scientific research institutes.
基金Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630062019003,19CXTD-31)Youth Foundation of Natural Science Foundation of Hainan Province(719QN282).
文摘In recent years,the Internet of Things(IoT)technology has been widely used in the production and sales of tropical fruits,with strong practicability and wide application prospects.The tropical fruit dynamic pricing model based on the IoT technology can promote the healthy development of the tropical fruit industry in Hainan and ensure the income of fruit farmers.Based on IoT technology,the quality grade of tropical fruits in Hainan is obtained.According to the dynamic pricing strategy of revenue management,a dynamic pricing model based on the quality of tropical fruits and a dynamic pricing model based on consumer segmentation are established to study the dynamic pricing problem under the condition of maximum profit for tropical fruit sellers.The research results show that for different fruit quality and consumer groups,different pricing models are required for pricing,in order to get the maximum profit from tropical fruit sales.Sellers must flexibly adopt different dynamic pricing models to price tropical fruits to enhance the competitiveness of the tropical fruit industry.
